A. Lacey Samuels, Thomas H. Giddings, L. Andrew Staehelin
Abstract. Cell plate formation in tobacco root tips and synchronized dividing suspension cultured tobacco BY-2 cells was examined using cryofixation and immu-nocytochemical methods. Due to the much...
-Catenin is a Nek2 substrate involved in centrosome separation (2008)
Bahmanyar, Shirin, Kaplan, Daniel D., DeLuca, Jennifer G., Giddings, Thomas H., OToole, Eileen T., Winey, Mark, ...
β-Catenin plays important roles in cell adhesion and gene transcription, and has been shown recently to be essential for the establishment of a bipolar mitotic spindle. Here we show that β-catenin...
BELIAKOVA-BETHELL, NADEJDA, BECKHAM, CARLA, GIDDINGS, THOMAS H., WINEY, MARK, PARKER, ROY, SANDMEYER, SUZANNE
Retroviruses and retrotransposons assemble intracellular immature core particles around a RNA genome, and nascent particles collect in association with membranes or as intracellular clusters. How and...
Subversion of Cellular Autophagosomal Machinery by RNA Viruses (2005)
William T. Jackson, Thomas H. Giddings, Matthew P. Taylor, Sara Mulinyawe, Marlene Rabinovitch, Ron R. Kopito, ...
Poliovirus is a well-studied positive-strand RNA virus, and here it is shown to subvert the machinery of the cellular autophagy pathway at several stages in the viral life cycle.
Murata, Takashi, Karahara, Ichirou, Kozuka, Toshiaki, Giddings, Thomas H., Staehelin, L. Andrew, Mineyuki, Yoshinobu
We have optimized the conditions for visualizing microfilaments, microtubules, and coated pits in the cortical cytoplasm of high‐pressure frozen and freeze‐substituted plant cells. In...
Mark Winey, Defne Yarar, Thomas H. Giddings, David N. Mastronarde
number of nuclear pore complexes (NPCs) in individual nuclei of the yeast Saccharomyces cerevisiae was determined by computer-aided reconstruction of entire nuclei from electron micrographs of...
Straight, Paul D., Giddings, Thomas H., Winey, Mark
Sporulation in yeast requires that a modified form of chromosome segregation be coupled to the development of a specialized cell type, a process akin to gametogenesis. Mps1p is a dual-specificity...
Jones, Michele H., Bachant, Jeffrey B., Castillo, Andrea R., Giddings, Thomas H., Winey, Mark
We have identified a mutant allele of the DAM1 gene in a screen for mutations that are lethal in combination with the mps1-1 mutation. MPS1 encodes an essential protein kinase that is required for...
Winey, Mark, Yarar, Defne, Giddings, Thomas H., Mastronarde, David N.
The number of nuclear pore complexes (NPCs) in individual nuclei of the yeast Saccharomyces cerevisiae was determined by computer-aided reconstruction of entire nuclei from electron micrographs of...
Yeast Dam1p has a role at the kinetochore in assembly of the mitotic spindle
Jones, Michele H., He, Xiangwei, Giddings, Thomas H., Winey, Mark
During mitosis, replicated chromosomes are separated to daughter cells by the microtubule-based mitotic spindle. Chromosomes attach to the mitotic spindle through specialized DNA/protein structures...
Saccharomyces cerevisiae Mob1p Is Required for Cytokinesis and Mitotic Exit
Luca, Francis C., Mody, Manali, Kurischko, Cornelia, Roof, David M., Giddings, Thomas H., Winey, Mark
The Saccharomyces cerevisiae mitotic exit network (MEN) is a conserved set of genes that mediate the transition from mitosis to G1 by regulating mitotic cyclin degradation and the inactivation of...
Suhy, David A., Giddings, Thomas H., Kirkegaard, Karla
All positive-strand RNA viruses of eukaryotes studied assemble RNA replication complexes on the surfaces of cytoplasmic membranes. Infection of mammalian cells with poliovirus and other...
Dodd, Dana A., Giddings, Thomas H., Kirkegaard, Karla
During viral infections, the host secretory pathway is crucial for both innate and acquired immune responses. For example, the export of most proinflammatory and antiviral cytokines, which recruit...
O'Toole, Eileen T., Giddings, Thomas H., McIntosh, J. Richard, Dutcher, Susan K.
Improved methods of specimen preparation and dual-axis electron tomography have been used to study the structure and organization of basal bodies in the unicellular alga Chlamydomonas reinhardtii....
Giddings, Thomas H., Cantino, Edward C.
A d-glucosamine 6-phosphate N-acetyltransferase from Blastocladiella emersonii zoospores was partially purified to a specific activity of 2.41 IU per mg of protein. Its pH optimum was 8.05 and its Km...
Giddings, Thomas H., Withers, Nancy W., Staehelin, L. Andrew
Freeze-fracture replicas of the photosynthetic prokaryote Prochloron sp., collected at Coconut Island, Hawaii, show that the thylakoids are differentiated into stacked and unstacked regions much like...
Requirement for Bbp1p in the Proper Mitotic Functions of Cdc5p in Saccharomyces cerevisiae
Park, Chong J., Song, Sukgil, Giddings, Thomas H., Ro, Hyeon-Su, Sakchaisri, Krisada, Park, Jung-Eun, ...
The polo-box domain of the budding yeast polo kinase Cdc5p plays an essential role for targeting the catalytic activity of Cdc5p to spindle pole bodies (SPBs) and cytokinetic neck-filaments. Here, we...
Lau, Corine K., Giddings, Thomas H., Winey, Mark
Both the spindle pole body (SPB) and the nuclear pore complex (NPC) are essential organelles embedded in the nuclear envelope throughout the life cycle of the budding yeast Saccharomyces cerevisiae....
Structure of the Thylakoids and Envelope Membranes of the Cyanelles of Cyanophora paradoxa1
Giddings, Thomas H., Wasmann, Cathy, Staehelin, L. Andrew
The cyanelles of Cyanophora paradoxa Korsch. are photosynthetically active obligate endosymbionts in which phycobiliproteins serve as the major accessory pigments. Freeze-fracture electron...
Subversion of Cellular Autophagosomal Machinery by RNA Viruses
Jackson, William T, Giddings, Thomas H, Taylor, Matthew P, Mulinyawe, Sara, Rabinovitch, Marlene, Kopito, Ron R, ...
Infection of human cells with poliovirus induces the proliferation of double-membraned cytoplasmic vesicles whose surfaces are used as the sites of viral RNA replication and whose origin is unknown....
Stemm-Wolf, Alexander J., Morgan, Garry, Giddings, Thomas H., White, Erin A., Marchione, Robb, McDonald, Heather B., ...
Centrins, small calcium binding EF-hand proteins, function in the duplication of a variety of microtubule organizing centers. These include centrioles in humans, basal bodies in green algae, and...
Three-dimensional Ultrastructure of Saccharomyces cerevisiae Meiotic SpindlesD⃞V⃞
Winey, Mark, Morgan, Garry P., Straight, Paul D., Giddings, Thomas H., Mastronarde, David N.
Meiotic chromosome segregation leads to the production of haploid germ cells. During meiosis I (MI), the paired homologous chromosomes are separated. Meiosis II (MII) segregation leads to the...
Araki, Yasuhiro, Lau, Corine K., Maekawa, Hiromi, Jaspersen, Sue L., Giddings, Thomas H., Schiebel, Elmar, ...
The spindle pole body (SPB) in Saccharomyces cerevisiae functions to nucleate and organize spindle microtubules, and it is embedded in the nuclear envelope throughout the yeast life cycle. However,...
Virus-like particles of the Ty3 retrotransposon assemble in association with P-body components
BELIAKOVA-BETHELL, NADEJDA, BECKHAM, CARLA, GIDDINGS, THOMAS H., WINEY, MARK, PARKER, ROY, SANDMEYER, SUZANNE
Retroviruses and retrotransposons assemble intracellular immature core particles around a RNA genome, and nascent particles collect in association with membranes or as intracellular clusters. How and...
Straight, Paul D., Giddings, Thomas H., Winey, Mark
Sporulation in yeast requires that a modified form of chromosome segregation be coupled to the development of a specialized cell type, a process akin to gametogenesis. Mps1p is a dual-specificity...
Jones, Michele H., Bachant, Jeffrey B., Castillo, Andrea R., Giddings, Thomas H., Winey, Mark
We have identified a mutant allele of the DAM1 gene in a screen for mutations that are lethal in combination with the mps1-1 mutation. MPS1 encodes an essential protein kinase that is required for...
Winey, Mark, Yarar, Defne, Giddings, Thomas H., Mastronarde, David N.
The number of nuclear pore complexes (NPCs) in individual nuclei of the yeast Saccharomyces cerevisiae was determined by computer-aided reconstruction of entire nuclei from electron micrographs of...
Yeast Dam1p has a role at the kinetochore in assembly of the mitotic spindle
Jones, Michele H., He, Xiangwei, Giddings, Thomas H., Winey, Mark
During mitosis, replicated chromosomes are separated to daughter cells by the microtubule-based mitotic spindle. Chromosomes attach to the mitotic spindle through specialized DNA/protein structures...
Saccharomyces cerevisiae Mob1p Is Required for Cytokinesis and Mitotic Exit
Luca, Francis C., Mody, Manali, Kurischko, Cornelia, Roof, David M., Giddings, Thomas H., Winey, Mark
The Saccharomyces cerevisiae mitotic exit network (MEN) is a conserved set of genes that mediate the transition from mitosis to G1 by regulating mitotic cyclin degradation and the inactivation of...
Suhy, David A., Giddings, Thomas H., Kirkegaard, Karla
All positive-strand RNA viruses of eukaryotes studied assemble RNA replication complexes on the surfaces of cytoplasmic membranes. Infection of mammalian cells with poliovirus and other...
Dodd, Dana A., Giddings, Thomas H., Kirkegaard, Karla
During viral infections, the host secretory pathway is crucial for both innate and acquired immune responses. For example, the export of most proinflammatory and antiviral cytokines, which recruit...
O'Toole, Eileen T., Giddings, Thomas H., McIntosh, J. Richard, Dutcher, Susan K.
Improved methods of specimen preparation and dual-axis electron tomography have been used to study the structure and organization of basal bodies in the unicellular alga Chlamydomonas reinhardtii....
Giddings, Thomas H., Cantino, Edward C.
A d-glucosamine 6-phosphate N-acetyltransferase from Blastocladiella emersonii zoospores was partially purified to a specific activity of 2.41 IU per mg of protein. Its pH optimum was 8.05 and its Km...
Giddings, Thomas H., Withers, Nancy W., Staehelin, L. Andrew
Freeze-fracture replicas of the photosynthetic prokaryote Prochloron sp., collected at Coconut Island, Hawaii, show that the thylakoids are differentiated into stacked and unstacked regions much like...
Requirement for Bbp1p in the Proper Mitotic Functions of Cdc5p in Saccharomyces cerevisiae
Park, Chong J., Song, Sukgil, Giddings, Thomas H., Ro, Hyeon-Su, Sakchaisri, Krisada, Park, Jung-Eun, ...
The polo-box domain of the budding yeast polo kinase Cdc5p plays an essential role for targeting the catalytic activity of Cdc5p to spindle pole bodies (SPBs) and cytokinetic neck-filaments. Here, we...
Lau, Corine K., Giddings, Thomas H., Winey, Mark
Both the spindle pole body (SPB) and the nuclear pore complex (NPC) are essential organelles embedded in the nuclear envelope throughout the life cycle of the budding yeast Saccharomyces cerevisiae....
Three-dimensional Ultrastructure of Saccharomyces cerevisiae Meiotic SpindlesD⃞V⃞
Winey, Mark, Morgan, Garry P., Straight, Paul D., Giddings, Thomas H., Mastronarde, David N.
Meiotic chromosome segregation leads to the production of haploid germ cells. During meiosis I (MI), the paired homologous chromosomes are separated. Meiosis II (MII) segregation leads to the...
Structure of the Thylakoids and Envelope Membranes of the Cyanelles of Cyanophora paradoxa1
Giddings, Thomas H., Wasmann, Cathy, Staehelin, L. Andrew
The cyanelles of Cyanophora paradoxa Korsch. are photosynthetically active obligate endosymbionts in which phycobiliproteins serve as the major accessory pigments. Freeze-fracture electron...
Subversion of Cellular Autophagosomal Machinery by RNA Viruses
Jackson, William T, Giddings, Thomas H, Taylor, Matthew P, Mulinyawe, Sara, Rabinovitch, Marlene, Kopito, Ron R, ...
Infection of human cells with poliovirus induces the proliferation of double-membraned cytoplasmic vesicles whose surfaces are used as the sites of viral RNA replication and whose origin is unknown....
Stemm-Wolf, Alexander J., Morgan, Garry, Giddings, Thomas H., White, Erin A., Marchione, Robb, McDonald, Heather B., ...
Centrins, small calcium binding EF-hand proteins, function in the duplication of a variety of microtubule organizing centers. These include centrioles in humans, basal bodies in green algae, and...
Virus-like particles of the Ty3 retrotransposon assemble in association with P-body components
BELIAKOVA-BETHELL, NADEJDA, BECKHAM, CARLA, GIDDINGS, THOMAS H., WINEY, MARK, PARKER, ROY, SANDMEYER, SUZANNE
Retroviruses and retrotransposons assemble intracellular immature core particles around a RNA genome, and nascent particles collect in association with membranes or as intracellular clusters. How and...
Araki, Yasuhiro, Lau, Corine K., Maekawa, Hiromi, Jaspersen, Sue L., Giddings, Thomas H., Schiebel, Elmar, ...
The spindle pole body (SPB) in Saccharomyces cerevisiae functions to nucleate and organize spindle microtubules, and it is embedded in the nuclear envelope throughout the yeast life cycle. However,...
Novel sfi1 Alleles Uncover Additional Functions for Sfi1p in Bipolar Spindle Assembly and Function
Anderson, Victoria E., Prudden, John, Prochnik, Simon, Giddings, Thomas H., Hardwick, Kevin G.
A variety of spindle and kinetochore defects have been shown to induce a mitotic delay through activation of the spindle checkpoint. With the aim of identifying novel mitotic defects we carried out a...
Jaspersen, Sue L., Martin, Adriana E., Glazko, Galina, Giddings, Thomas H., Morgan, Garry, Mushegian, Arcady, ...
The spindle pole body (SPB) is the sole site of microtubule nucleation in Saccharomyces cerevisiae; yet, details of its assembly are poorly understood. Integral membrane proteins including Mps2...
New Tetrahymena basal body protein components identify basal body domain structure
Kilburn, Chandra L., Pearson, Chad G., Romijn, Edwin P., Meehl, Janet B., Giddings, Thomas H., Culver, Brady P., ...
Basal bodies organize the nine doublet microtubules found in cilia. Cilia are required for a variety of cellular functions, including motility and sensing stimuli. Understanding this biochemically...
New Tetrahymena basal body protein components identify basal body domain structure
Kilburn, Chandra L., Pearson, Chad G., Romijn, Edwin P., Meehl, Janet B., Giddings, Thomas H., Culver, Brady P., ...
Schutz, Amy R., Giddings, Thomas H., Steiner, Estelle, Winey, Mark
The MPS1 gene from Saccharomyces cerevisiae encodes an essential protein kinase required for spindle pole body (SPB) duplication and for the mitotic spindle assembly checkpoint. Cells with the mps1-1...
Jaspersen, Sue L., Giddings, Thomas H., Winey, Mark
Accurate duplication of the Saccharomyces cerevisiae spindle pole body (SPB) is required for formation of a bipolar mitotic spindle. We identified mutants in SPB assembly by screening a...
Chial, Heidi J., Rout, Michael P., Giddings, Thomas H., Winey, Mark
We report a novel connection between nuclear pore complexes (NPCs) and spindle pole bodies (SPBs) revealed by our studies of the Saccharomyces cerevisiae NDC1 gene. Although both NPCs and SPBs are...
β-Catenin is a Nek2 substrate involved in centrosome separation
Bahmanyar, Shirin, Kaplan, Daniel D., DeLuca, Jennifer G., Giddings, Thomas H., O’Toole, Eileen T., Winey, Mark, ...
β-Catenin plays important roles in cell adhesion and gene transcription, and has been shown recently to be essential for the establishment of a bipolar mitotic spindle. Here we show that β-catenin...
Basal Body Components Exhibit Differential Protein Dynamics during Nascent Basal Body Assembly
Pearson, Chad G., Giddings, Thomas H., Winey, Mark
Basal bodies organize cilia that are responsible for both mechanical beating and sensation. Nascent basal body assembly follows a series of well characterized morphological events; however, the...
The Two SAS-6 Homologs in Tetrahymena thermophila Have Distinct Functions in Basal Body Assembly
Culver, Brady P., Meehl, Janet B., Giddings, Thomas H., Winey, Mark
Cilia and flagella are structurally and functionally conserved organelles present in basal as well as higher eukaryotes. The assembly of cilia requires a microtubule based scaffold called a basal...