Thomas Harasim

The BRCT domain of mammalian Pes1 is crucial for nucleolar localization and rRNA processing (2007)

Hölzel, Michael, Grimm, Thomas, Rohrmoser, Michaela, Malamoussi, Anastassia, Harasim, Thomas, Gruber-Eber, Anita, ...

The nucleolar protein Pes1 interacts with Bop1 and WDR12 in a stable complex (PeBoW-complex) and its expression is tightly associated with cell proliferation. The yeast homologue Nop7p (Yph1p)...

Rapid conditional knock-down-knock-in system for mammalian cells (2007)

Hölzel, Michael, Rohrmoser, Michaela, Orban, Mathias, Hömig, Cornelia, Harasim, Thomas, Malamoussi, Anastassia, ...

RNA interference (RNAi) is a powerful tool to analyze gene function in mammalian cells. However, the interpretation of RNAi knock-down phenotypes can be hampered by off-target effects or compound...

Dominant-negative Pes1 mutants inhibit ribosomal RNA processing and cell proliferation via incorporation into the PeBoW-complex (2006)

Grimm, Thomas, Hölzel, Michael, Rohrmoser, Michaela, Harasim, Thomas, Malamoussi, Anastassia, Gruber-Eber, Anita, ...

The nucleolar PeBoW-complex, consisting of Pes1, Bop1 and WDR12, is essential for cell proliferation and processing of ribosomal RNA in mammalian cells. Here we have analysed the physical and...

Rapid conditional knock-down-knock-in system for mammalian cells (2006)

Hölzel, Michael, Rohrmoser, Michaela, Orban, Mathias, Hömig, Cornelia, Harasim, Thomas, Malamoussi, Anastassia, ...

RNA interference (RNAi) is a powerful tool to analyze gene function in mammalian cells. However, the interpretation of RNAi knock-down phenotypes can be hampered by off-target effects or compound...

The BRCT domain of mammalian Pes1 is crucial for nucleolar localization and rRNA processing (2006)

Hölzel, Michael, Grimm, Thomas, Rohrmoser, Michaela, Malamoussi, Anastassia, Harasim, Thomas, Gruber-Eber, Anita, ...

The nucleolar protein Pes1 interacts with Bop1 and WDR12 in a stable complex (PeBoW-complex) and its expression is tightly associated with cell proliferation. The yeast homologue Nop7p (Yph1p)...

Rapid conditional knock-down-knock-in system for mammalian cells (2006)

Hölzel, Michael, Rohrmoser, Michaela, Orban, Mathias, Hömig, Cornelia, Harasim, Thomas, Malamoussi, Anastassia, ...

RNA interference (RNAi) is a powerful tool to analyze gene function in mammalian cells. However, the interpretation of RNAi knock-down phenotypes can be hampered by off-target effects or compound...

The BRCT domain of mammalian Pes1 is crucial for nucleolar localization and rRNA processing (2006)

Hölzel, Michael, Grimm, Thomas, Rohrmoser, Michaela, Malamoussi, Anastassia, Harasim, Thomas, Gruber-Eber, Anita, ...

The nucleolar protein Pes1 interacts with Bop1 and WDR12 in a stable complex (PeBoW-complex) and its expression is tightly associated with cell proliferation. The yeast homologue Nop7p (Yph1p)...

Dominant-negative Pes1 mutants inhibit ribosomal RNA processing and cell proliferation via incorporation into the PeBoW-complex

Grimm, Thomas, Hölzel, Michael, Rohrmoser, Michaela, Harasim, Thomas, Malamoussi, Anastassia, Gruber-Eber, Anita, ...

The nucleolar PeBoW-complex, consisting of Pes1, Bop1 and WDR12, is essential for cell proliferation and processing of ribosomal RNA in mammalian cells. Here we have analysed the physical and...

Dominant-negative Pes1 mutants inhibit ribosomal RNA processing and cell proliferation via incorporation into the PeBoW-complex

Grimm, Thomas, Hölzel, Michael, Rohrmoser, Michaela, Harasim, Thomas, Malamoussi, Anastassia, Gruber-Eber, Anita, ...

The nucleolar PeBoW-complex, consisting of Pes1, Bop1 and WDR12, is essential for cell proliferation and processing of ribosomal RNA in mammalian cells. Here we have analysed the physical and...

Rapid conditional knock-down–knock-in system for mammalian cells

Hölzel, Michael, Rohrmoser, Michaela, Orban, Mathias, Hömig, Cornelia, Harasim, Thomas, Malamoussi, Anastassia, ...

RNA interference (RNAi) is a powerful tool to analyze gene function in mammalian cells. However, the interpretation of RNAi knock-down phenotypes can be hampered by off-target effects or compound...

The BRCT domain of mammalian Pes1 is crucial for nucleolar localization and rRNA processing

Hölzel, Michael, Grimm, Thomas, Rohrmoser, Michaela, Malamoussi, Anastassia, Harasim, Thomas, Gruber-Eber, Anita, ...

The nucleolar protein Pes1 interacts with Bop1 and WDR12 in a stable complex (PeBoW-complex) and its expression is tightly associated with cell proliferation. The yeast homologue Nop7p (Yph1p)...

Interdependence of Pes1, Bop1, and WDR12 Controls Nucleolar Localization and Assembly of the PeBoW Complex Required for Maturation of the 60S Ribosomal Subunit▿

Rohrmoser, Michaela, Hölzel, Michael, Grimm, Thomas, Malamoussi, Anastassia, Harasim, Thomas, Orban, Mathias, ...

The PeBoW complex is essential for cell proliferation and maturation of the large ribosomal subunit in mammalian cells. Here we examined the role of PeBoW-specific proteins Pes1, Bop1, and WDR12 in...

Mammalian WDR12 is a novel member of the Pes1–Bop1 complex and is required for ribosome biogenesis and cell proliferation

Hölzel, Michael, Rohrmoser, Michaela, Schlee, Martin, Grimm, Thomas, Harasim, Thomas, Malamoussi, Anastassia, ...

Target genes of the protooncogene c-myc are implicated in cell cycle and growth control, yet the linkage of both is still unexplored. Here, we show that the products of the nucleolar target genes...

The nucleolar SUMO-specific protease SENP3 reverses SUMO modification of nucleophosmin and is required for rRNA processing

Haindl, Markus, Harasim, Thomas, Eick, Dirk, Muller, Stefan

The ubiquitin-like SUMO system functions by a cyclic process of modification and demodification, and recent data suggest that the nucleolus is a site of sumoylation–desumoylation cycles. For...