Thomas J. Montville

Functional Characterization of Pediocin PA-1 Binding to Liposomes in the Absence of a Protein Receptor and Its Relationship to a Predicted Tertiary Structure† (1996)

Yuhuan Chen, Roni Shapira, Miriam Eisenstein, Thomas J. Montville

The physicochemical interaction of pediocin PA-1 with target membranes was characterized using lipid vesicles made from the total lipids extracted from Listeria monocytogenes. Pediocin PA-1 caused...

Food microbiology / edited by Thomas J. Montville (1987)

Montville, Thomas J.

"This book is publication no. C-101112-2-85 of the New Jersey Agricultural Experiment Station"

Characterization of Fatty Acid Composition, Spore Germination, and Thermal Resistance in a Nisin-Resistant Mutant of Clostridium botulinum 169B and in the Wild-Type Strain†

Mazzotta, Alejandro S., Montville, Thomas J.

The membrane fatty acids, thermal resistance, and germination of a nisin-resistant (Nisr) mutant of Clostridium botulinum 169B were compared with those of the wild-type (WT) strain. In the membranes...

Carbon Dioxide and Nisin Act Synergistically on Listeria monocytogenes

Nilsson, Lilian, Chen, Yuhuan, Chikindas, Michael L., Huss, Hans Henrik, Gram, Lone, Montville, Thomas J.

This paper examines the synergistic action of carbon dioxide and nisin on Listeria monocytogenes Scott A wild-type and nisin-resistant (Nisr) cells grown in broth at 4°C. Carbon dioxide extended the...

Analysis of the Influence of Environmental Parameters on Clostridium botulinum Time-to-Toxicity by Using Three Modeling Approaches†

Schaffner, Donald W., Ross, William H., Montville, Thomas J.

This study used the technique of waiting time modeling to analyze the combined effects of temperature, pH, carbohydrate, protein, and lipid on the time-to-toxicity of Clostridium botulinum 56A....

Influence of Lipid Composition on Pediocin PA-1 Binding to Phospholipid Vesicles†

Chen, Yuhuan, Ludescher, Richard D., Montville, Thomas J.

Pediocin PA-1 bound to anionic lipid vesicles with saturated or unsaturated fatty acid chains in a lipid concentration-dependent fashion. Little change in binding parameters was observed for...

Nisin Resistance in Listeria monocytogenes ATCC 700302 Is a Complex Phenotype†

Crandall, Allison D., Montville, Thomas J.

Nisin resistance in Listeria monocytogenes ATCC 700302 is a complex phenotype involving alterations in both the cytoplasmic membrane and the cell wall and a requirement for divalent cations. In...

Temperature- and Surfactant-Induced Membrane Modifications That Alter Listeria monocytogenes Nisin Sensitivity by Different Mechanisms†

Li, Jie, Chikindas, Michael L., Ludescher, Richard D., Montville, Thomas J.

Nisin interacts with target membranes in four sequential steps: binding, insertion, aggregation, and pore formation. Alterations in membrane composition might influence any of these steps. We...

Common Mechanistic Action of Bacteriocins from Lactic Acid Bacteria †

Bruno, Maria E. C., Montville, Thomas J.

The influence of four bacteriocins from lactic acid bacteria on the proton motive force (PMF) of sensitive organisms was investigated. Pediocin PA-1 (20 μg/ml) and leuconocin S (48.5 μg/ml)...

Enzyme Activities Affecting End Product Distribution by Lactobacillus plantarum in Response to Changes in pH and O2†

Tseng, Ching-Ping, Montville, Thomas J.

Lactobacillus plantarum catabolic end products changed in response to environmental conditions. While lactate was always the major end product, acetate was produced in alkaline and aerobic...

Production of an Amylase-Sensitive Bacteriocin by an Atypical Leuconostoc paramesenteroides Strain †

Lewus, Catherine B., Sun, Stephen, Montville, Thomas J.

An atypical Leuconostoc paramesenteroides strain isolated from retail lamb produced a bacteriocin, leuconocin S, that was inactivated by α-amylase, trypsin, α-chymotrypsin, protease, and proteinase...

Conversion of Pyruvate to Acetoin Helps To Maintain pH Homeostasis in Lactobacillus plantarum†

Tsau, Jya-Li, Guffanti, Arthur A., Montville, Thomas J.

Pyruvate is the substrate for diacetyl and acetoin synthesis by lactobacilli. Exogenous pyruvate stimulates acetoin production when glucose is present as an energy source. In Lactobacillus plantarum...

High-Efficiency Conversion of Pyruvate to Acetoin by Lactobacillus plantarum during pH-Controlled and Fed-Batch Fermentations †

Montville, Thomas J., Hsu, Amy Han-Ming, Meyer, Mary E.

The influence of pH on the type and concentration of metabolites produced from pyruvate by Lactobacillus plantarum ATCC 8014 was examined in pH-controlled fermentors at pH values of 4.5 to 6.5....

Dual-Substrate Plate Diffusion Assay for Proteases

Montville, Thomas J.

A plate diffusion assay for endopeptidases was developed. Proteases applied to plates containing 1% casein, 1% gelatin, and 1.5% agar caused distinct zones reminiscent of immunoprecipitation bands....

Effect of Plating Medium on Heat Activation Requirement of Clostridium botulinum Spores

Montville, Thomas J.

Clostridium botulinum 62A and ATCC 25763 spores required heat activation for maximum colony formation when plated on reinforced clostridial agar (BBL Microbiology Systems) but not when plated on...

Increased ATPase Activity Is Responsible for Acid Sensitivity of Nisin-Resistant Listeria monocytogenes ATCC 700302

McEntire, Jennifer Cleveland, Carman, George M., Montville, Thomas J.

The growth of the foodborne pathogen Listeria monocytogenes can be controlled by nisin, an antimicrobial peptide. A spontaneous mutant of L. monocytogenes shows both resistance to nisin and increased...

Purification and Characterization of a Novel Class IIa Bacteriocin, Piscicocin CS526, from Surimi-Associated Carnobacterium piscicola CS526

Yamazaki, Koji, Suzuki, Minako, Kawai, Yuji, Inoue, Norio, Montville, Thomas J.

The bacteriocin piscicocin CS526 was inactivated by proteolytic enzymes, was stable at 100°C for 30 min, had a pH range of 2 to 8, and was active against Enterococcus, Listeria, Pediococcus, and...

Bioenergetic Mechanism for Nisin Resistance, Induced by the Acid Tolerance Response of Listeria monocytogenes

Bonnet, Marcelo, Rafi, Mohamed M., Chikindas, Michael L., Montville, Thomas J.

This study examined the bioenergetics of Listeria monocytogenes, induced to an acid tolerance response (ATR). Changes in bioenergetic parameters were consistent with the increased resistance of...

Characterization of Fatty Acid Composition, Spore Germination, and Thermal Resistance in a Nisin-Resistant Mutant of Clostridium botulinum 169B and in the Wild-Type Strain†

Mazzotta, Alejandro S., Montville, Thomas J.

The membrane fatty acids, thermal resistance, and germination of a nisin-resistant (Nisr) mutant of Clostridium botulinum 169B were compared with those of the wild-type (WT) strain. In the membranes...

Carbon Dioxide and Nisin Act Synergistically on Listeria monocytogenes

Nilsson, Lilian, Chen, Yuhuan, Chikindas, Michael L., Huss, Hans Henrik, Gram, Lone, Montville, Thomas J.

This paper examines the synergistic action of carbon dioxide and nisin on Listeria monocytogenes Scott A wild-type and nisin-resistant (Nisr) cells grown in broth at 4°C. Carbon dioxide extended the...

Analysis of the Influence of Environmental Parameters on Clostridium botulinum Time-to-Toxicity by Using Three Modeling Approaches†

Schaffner, Donald W., Ross, William H., Montville, Thomas J.

This study used the technique of waiting time modeling to analyze the combined effects of temperature, pH, carbohydrate, protein, and lipid on the time-to-toxicity of Clostridium botulinum 56A....

Influence of Lipid Composition on Pediocin PA-1 Binding to Phospholipid Vesicles†

Chen, Yuhuan, Ludescher, Richard D., Montville, Thomas J.

Pediocin PA-1 bound to anionic lipid vesicles with saturated or unsaturated fatty acid chains in a lipid concentration-dependent fashion. Little change in binding parameters was observed for...

Nisin Resistance in Listeria monocytogenes ATCC 700302 Is a Complex Phenotype†

Crandall, Allison D., Montville, Thomas J.

Nisin resistance in Listeria monocytogenes ATCC 700302 is a complex phenotype involving alterations in both the cytoplasmic membrane and the cell wall and a requirement for divalent cations. In...

Temperature- and Surfactant-Induced Membrane Modifications That Alter Listeria monocytogenes Nisin Sensitivity by Different Mechanisms†

Li, Jie, Chikindas, Michael L., Ludescher, Richard D., Montville, Thomas J.

Nisin interacts with target membranes in four sequential steps: binding, insertion, aggregation, and pore formation. Alterations in membrane composition might influence any of these steps. We...

Common Mechanistic Action of Bacteriocins from Lactic Acid Bacteria †

Bruno, Maria E. C., Montville, Thomas J.

The influence of four bacteriocins from lactic acid bacteria on the proton motive force (PMF) of sensitive organisms was investigated. Pediocin PA-1 (20 μg/ml) and leuconocin S (48.5 μg/ml)...

Enzyme Activities Affecting End Product Distribution by Lactobacillus plantarum in Response to Changes in pH and O2†

Tseng, Ching-Ping, Montville, Thomas J.

Lactobacillus plantarum catabolic end products changed in response to environmental conditions. While lactate was always the major end product, acetate was produced in alkaline and aerobic...

Production of an Amylase-Sensitive Bacteriocin by an Atypical Leuconostoc paramesenteroides Strain †

Lewus, Catherine B., Sun, Stephen, Montville, Thomas J.

An atypical Leuconostoc paramesenteroides strain isolated from retail lamb produced a bacteriocin, leuconocin S, that was inactivated by α-amylase, trypsin, α-chymotrypsin, protease, and proteinase...

Conversion of Pyruvate to Acetoin Helps To Maintain pH Homeostasis in Lactobacillus plantarum†

Tsau, Jya-Li, Guffanti, Arthur A., Montville, Thomas J.

Pyruvate is the substrate for diacetyl and acetoin synthesis by lactobacilli. Exogenous pyruvate stimulates acetoin production when glucose is present as an energy source. In Lactobacillus plantarum...

High-Efficiency Conversion of Pyruvate to Acetoin by Lactobacillus plantarum during pH-Controlled and Fed-Batch Fermentations †

Montville, Thomas J., Hsu, Amy Han-Ming, Meyer, Mary E.

The influence of pH on the type and concentration of metabolites produced from pyruvate by Lactobacillus plantarum ATCC 8014 was examined in pH-controlled fermentors at pH values of 4.5 to 6.5....

Dual-Substrate Plate Diffusion Assay for Proteases

Montville, Thomas J.

A plate diffusion assay for endopeptidases was developed. Proteases applied to plates containing 1% casein, 1% gelatin, and 1.5% agar caused distinct zones reminiscent of immunoprecipitation bands....

Effect of Plating Medium on Heat Activation Requirement of Clostridium botulinum Spores

Montville, Thomas J.

Clostridium botulinum 62A and ATCC 25763 spores required heat activation for maximum colony formation when plated on reinforced clostridial agar (BBL Microbiology Systems) but not when plated on...

Increased ATPase Activity Is Responsible for Acid Sensitivity of Nisin-Resistant Listeria monocytogenes ATCC 700302

McEntire, Jennifer Cleveland, Carman, George M., Montville, Thomas J.

The growth of the foodborne pathogen Listeria monocytogenes can be controlled by nisin, an antimicrobial peptide. A spontaneous mutant of L. monocytogenes shows both resistance to nisin and increased...

Purification and Characterization of a Novel Class IIa Bacteriocin, Piscicocin CS526, from Surimi-Associated Carnobacterium piscicola CS526

Yamazaki, Koji, Suzuki, Minako, Kawai, Yuji, Inoue, Norio, Montville, Thomas J.

The bacteriocin piscicocin CS526 was inactivated by proteolytic enzymes, was stable at 100°C for 30 min, had a pH range of 2 to 8, and was active against Enterococcus, Listeria, Pediococcus, and...

Bioenergetic Mechanism for Nisin Resistance, Induced by the Acid Tolerance Response of Listeria monocytogenes

Bonnet, Marcelo, Rafi, Mohamed M., Chikindas, Michael L., Montville, Thomas J.

This study examined the bioenergetics of Listeria monocytogenes, induced to an acid tolerance response (ATR). Changes in bioenergetic parameters were consistent with the increased resistance of...

Changes in Listeria monocytogenes Membrane Fluidity in Response to Temperature Stress▿

Najjar, Mohamed Badaoui, Chikindas, Michael, Montville, Thomas J.

Listeria monocytogenes is a food-borne pathogen that has been implicated in many outbreaks associated with ready-to-eat products. Listeria adjusts to various stresses by adjusting its membrane...