Gisi, Daniel, Maillard, Julien, Flanagan, Jack U, Rossjohn, Jamie, Chelvanayagam, Gareth, Board, Philip G, ...
Methylobacterium dichloromethanicum DM4 is able to grow with dichloromethane as the sole carbon and energy source by using a dichloromethane dehalogenase/glutathione S-transferase (GST) for the...
Beil, Stefan, Kehrli, Hans, James, Peter, Staudenmann, Werner, Cook, Alasdair M., Leisinger, Thomas, ...
An arylsulfatase (EC 3.1.6.1) was extracted from Pseudomonas aeruginosa PAO1 and purified 2700-fold to homogeneity. Synthesis of this enzyme was repressed when sulfate, cysteine or thiocyanate was...
Schläfli, Hans R., Baker, Darren P., Leisinger, Thomas, Cook, Alasdair M.
The stereospecificity of hydride removal from the 4 position of the pyridine ring of NADH by reductases from all three classes of multicomponent nonheme iron oxygenases was examined. The class I and...
Microbial metabolism of sulfurand phosphorus-containing xenobiotics (1994)
Kertesz, Michael A., Cook, Alasdair M., Leisinger, Thomas
The enzymes involved in the microbial metabolism of many important phosphorus- or sulfur-containing xenobiotics, including organophosphate insecticides and precursors to organosulfate and...
Schläfli, Hans R., Weiss, Martina A., Leisinger, Thomas, Cook, Alasdair M.
Comamonas testosteroni T-2, grown in terephthalate (TER)-salts medium, synthesizes inducible enzymes that convert TER to (1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylic acid (DCD) and...
Junker, Frank, Field, Jennifer A., Bangerter, Felix, Ramsteiner, Klaus, Kohler, Hans-Peter, Joannou, Chris L., ...
2-Aminobenzenesulphonic acid (2AS) is degraded by Alcaligenes sp. strain O-1 via a previously detected but unidentified intermediate. A mutant of strain O-1 was found to excrete this intermediate,...
Biotransformation of Chloromethane to Methanethiol (1993)
Stromeyer, Susanna A., Cook, Alasdair M., Leisinger, Thomas
Monochloromethane (MeCl) was biotransformed by a mixed culture of anaerobic bacteria which had been enriched to utilize dichloromethane as the sole source of carbon and energy. Cells grown in...
Braus-Stromeyer, Susanna A., Hermann, René, Cook, Alasdair M., Leisinger, Thomas
Dichloromethane (DCM) is utilized by the strictly anaerobic, acetogenic mixed culture DM as a sole source of carbon and energy for growth. Growth with DCM was linear, and cell suspensions of the...
Kertesz, Michael A., Leisinger, Thomas, Cook, Alasdair M.
Two-dimensional gel electrophoresis of proteins from Escherichia coli, Pseudomonas putida, and Staphylococcus aureus, grown with methionine or one of a variety of organosulfates and organosulfonates...
Stromeyer, Susanna A., Stumpf, Karin, Cook, Alasdair M., Leisinger, Thomas
The transformations of 14CCl4 by whole cells of Acetobacterium woodii suspended in phosphate buffer containing reducing agents, and by the cobalt corrinoid aquocobalamin in the same solution, were...
Locher, Hans H., Leisinger, Thomas, Cook, Alasdair M.
Cell-free extracts of Comamonas testosteroni T-2 grown in toluene-p-sulphonate/salts medium catalyse the conversion of p-sulphobenzoate (PSB) into protocatechuate and sulphite by an NADH-requiring...
Locher, Hans H., Leisinger, Thomas, Cook, Alasdair M.
Comamonas testosteroni T-2 synthesizes an inducible enzyme system that oxygenates 4-toluene sulfontate (TS) to 4-sulfobenzyl alcohol when grown in TS-salts medium. We purified this TS...
Egli, Christine, Stromeyer, Susanna A., Cook, Alasdair M., Leisinger, Thomas
Abstract Degradation of tetrachloromethane was examined in the three strictly anaerobic bacteria, Acetobacterium woodii, Desulfobacterium autotrophicum and Methanobacterium thermoautotrophicum When...
Thurnheer, Thomas, Zürrer, Daniel, Höglinger, Otmar, Leisinger, Thomas, Cook, Alasdair M.
Alcaligenes sp. strain O-1 grew with benzene sulfonate (BS) as sole carbon source for growth with either NH4 + or NH4 + plus orthanilate (2-aminobenzene sulfonate, OS) as the source(s) of nitrogen....
Egli, Christine, Thüer, Markus, Suter, Dieter, Cook, Alasdair M., Leisinger, Thomas
A stable methanogenic mixed culture was enriched from an industrial environment to utilize chloroacetate as sole carbon and energy source for growth. It immobilized spontaneously on activated...
Locher, Hans H., Thurnheer, Thomas, Leisinger, Thomas, Cook, Alasdair M.
Aerobic, carbon-limited, enrichment cultures containing 3-aminobenzenesulfonate or 3-nitrobenzenesulfonate as the sole source of carbon and energy yielded growth and complete substrate disappearance....
Thurnheer, Thomas, Cook, Alasdair M., Leisinger, Thomas
A co-culture, consisting of five defined bacteria [e.g., T. Thurnheer, T. Köhler, A. M. Cook, and T. Leisinger: J Gen Microbiol 132:12151220], was able to degrade at least seven substituted...
Scholtz, Rudolf, Messi, Ferruccio, Leisinger, Thomas, Cook, Alasdair M.
Arthrobacter sp. strain HA1 utilizes 18 C2-to-C8 1-haloalkanes for growth and synthesizes an inducible 1-bromoalkane debrominase of unknown physiological function (R. Scholtz, T. Leisinger, F. Suter,...
Scholtz, Rudolf, Wackett, Lawrence P., Egli, Christine, Cook, Alasdair M., Leisinger, Thomas
A methylotrophic bacterium, denoted strain DM11, was isolated from groundwater and shown to utilize dichloromethane or dibromomethane as the sole carbon and energy source. The new isolate grew at the...
Egli, Christine, Tschan, Thomas, Scholtz, Rudolf, Cook, Alasdair M., Leisinger, Thomas
Five anaerobic bacteria were tested for their abilities to transform tetrachloromethane so that information about enzymes involved in reductive dehalogenations of polychloromethanes could be...
Egli, Christine, Scholtz, Rudolf, Cook, Alasdair M., Leisinger, Thomas
Methanobacterium thermoautotrophicum grew autotrophically and Desulfobacterium autotrophicum grew heterotrophically in the presence of one of several highly chlorinated aliphatic hydrocarbons in the...
Microbial desulfonation of substituted naphthalenesulfonic acids and benzenesulfonic acids (1987)
Zürrer, Daniel, Cook, Alasdair M., Leisinger, Thomas
Sulfur-limited batch enrichment cultures containing one of nine multisubstituted naphthalenesulfonates and an inoculum from sewage yielded several taxa of bacteria which could quantitatively utilize...
Scholtz, Rudolf, Leisinger, Thomas, Suter, Franz, Cook, Alasdair M.
1-Chlorohexane halidohydrolase from Arthrobacter sp. strain HA1 was purified to homogeneity by fractional precipitation, ion-exchange chromatography, gel filtration, and high-performance liquid...
A corrinoid-dependent catabolic pathway for growth of a Methylobacterium strain with chloromethane
Vannelli, Todd, Messmer, Michael, Studer, Alex, Vuilleumier, Stéphane, Leisinger, Thomas
Methylobacterium sp. strain CM4, an aerobic methylotrophic α-proteobacterium, is able to grow with chloromethane as a carbon and energy source. Mutants of this strain that still grew with methanol,...
McAnulla, Craig, Woodall, Claire A., McDonald, Ian R., Studer, Alex, Vuilleumier, Stephane, Leisinger, Thomas, ...
Hyphomicrobium chloromethanicum CM2T, an aerobic methylotrophic member of the α subclass of the class proteobacteria, can grow with chloromethane as the sole carbon and energy source. H....
Luo, Yongneng, Pfister, Peter, Leisinger, Thomas, Wasserfallen, Alain
Methanothermobacter wolfeii (formerly Methanobacterium wolfei), a thermophilic methanoarchaeon whose cultures lyse upon hydrogen starvation, carries a defective prophage called ΨM100 on its...
Hummerjohann, Jörg, Laudenbach, Sascha, Rétey, Julia, Leisinger, Thomas, Kertesz, Michael A.
A gene cluster upstream of the arylsulfatase gene (atsA) in Pseudomonas aeruginosa was characterized and found to encode a putative ABC-type transporter, AtsRBC. Mutants with insertions in the atsR...
Deletion Analysis of the Escherichia coli Taurine and Alkanesulfonate Transport Systems
Eichhorn, Eric, Van Der Ploeg, Jan R., Leisinger, Thomas
The Escherichia coli tauABCD and ssuEADCB gene clusters are required for the utilization of taurine and alkanesulfonates as sulfur sources and are expressed only under conditions of sulfate or...
The ssu Locus Plays a Key Role in Organosulfur Metabolism in Pseudomonas putida S-313
Kahnert, Antje, Vermeij, Paul, Wietek, Claudia, James, Peter, Leisinger, Thomas, Kertesz, Michael A.
Pseudomonas putida S-313 can utilize a broad range of aromatic sulfonates as sulfur sources for growth in sulfate-free minimal medium. The sulfonates are cleaved monooxygenolytically to yield the...
Metabolism of Dichloromethane by the Strict Anaerobe Dehalobacterium formicoaceticum
Mägli, Andreas, Messmer, Michael, Leisinger, Thomas
The metabolism of dichloromethane by Dehalobacterium formicoaceticum in cell suspensions and crude cell extracts was investigated. The organism is a strictly anaerobic gram-positive bacterium that...
Gisi, Daniel, Willi, Laurent, Traber, Hubert, Leisinger, Thomas, Vuilleumier, Stéphane
Methylobacterium sp. strain DM4 and Methylophilus sp. strain DM11 can grow with dichloromethane (DCM) as the sole source of carbon and energy by virtue of homologous glutathione-dependent DCM...
Chloromethane Metabolism by Methylobacterium sp. Strain CM4
Vannelli, Todd, Studer, Alex, Kertesz, Michael, Leisinger, Thomas
Methylobacterium sp. strain CM4 metabolized chloromethane quantitatively with a molar yield of 2.8 g of whole-cell protein/mol of C. This value was similar to that observed after growth with methanol...
De Azevedo Wäsch, Susana I., Van Der Ploeg, Jan R., Maire, Tere, Lebreton, Alice, Kiener, Andreas, Leisinger, Thomas
Pseudomonas sp. strain KIE171 was able to grow with isopropylamine or l-alaninol [S-(+)-2-amino-1-propanol] as the sole carbon source, but not with d-alaninol. To investigate the hypothesis that...
Studer, Alex, McAnulla, Craig, Büchele, Rainer, Leisinger, Thomas, Vuilleumier, Stéphane
Methylobacterium chloromethanicum CM4 is an aerobic α-proteobacterium capable of growth with chloromethane as the sole carbon and energy source. Two proteins, CmuA and CmuB, were previously purified...
Locher, Hans H., Thurnheer, Thomas, Leisinger, Thomas, Cook, Alasdair M.
Aerobic, carbon-limited, enrichment cultures containing 3-aminobenzenesulfonate or 3-nitrobenzenesulfonate as the sole source of carbon and energy yielded growth and complete substrate disappearance....
REPRESSION-DEPENDENT ALTERATION OF AN ARGININE ENZYME IN Escherichia coli
Leisinger, Thomas, Vogel, Ruth H., Vogel, Henry J.
Treatment of susceptible Escherichia coli K12 derivatives with 0.4 M Mg++ at 37°, potentiated by L-arginine or L-canavanine, leads to alteration of acetylornithine δ-transaminase. The alteration,...
Expression of the argA Gene Carried by a Defective Lambda Bacteriophage of Escherichia coli
Leisinger, Thomas, Haas, Dieter, Kelker, Norman
Evidence is presented that the increase in specific activity of N-acetylglutamate synthase observed upon heat induction of Escherichia coli (λdargA) is primarily due to a gene dosage effect.
Bacterial Degradation of Dichloromethane
Brunner, Walter, Staub, Doris, Leisinger, Thomas
Strain DM1, a facultative methylotrophic bacterium utilizing methanol, formate, mono-, di-, and trimethylamine, as well as dichloromethane as C1 substrates was isolated as an airborne contaminant....
N2-Succinylated intermediates in an arginine catabolic pathway of Pseudomonas aeruginosa
Jann, Alfred, Stalon, Victor, Wauven, Corinne Vander, Leisinger, Thomas, Haas, Dieter
Arginine-nonutilizing (aru) mutants of Pseudomonas aeruginosa strain PAO converted L-arginine to N2-succinylarginine or N-succinylglutamate, which were identified by high-voltage electrophoresis and...
Krishna, Rangachar V., Beilstein, Paul, Leisinger, Thomas
γ-Glutamyl phosphate reductase, the second enzyme of proline biosynthesis, catalyses the formation of l-glutamic acid 5-semialdehyde from γ-glutamyl phosphate with NAD(P)H as cofactor. It was...
A corrinoid-dependent catabolic pathway for growth of a Methylobacterium strain with chloromethane
Vannelli, Todd, Messmer, Michael, Studer, Alex, Vuilleumier, Stéphane, Leisinger, Thomas
Methylobacterium sp. strain CM4, an aerobic methylotrophic α-proteobacterium, is able to grow with chloromethane as a carbon and energy source. Mutants of this strain that still grew with methanol,...
McAnulla, Craig, Woodall, Claire A., McDonald, Ian R., Studer, Alex, Vuilleumier, Stephane, Leisinger, Thomas, ...
Hyphomicrobium chloromethanicum CM2T, an aerobic methylotrophic member of the α subclass of the class proteobacteria, can grow with chloromethane as the sole carbon and energy source. H....
Luo, Yongneng, Pfister, Peter, Leisinger, Thomas, Wasserfallen, Alain
Methanothermobacter wolfeii (formerly Methanobacterium wolfei), a thermophilic methanoarchaeon whose cultures lyse upon hydrogen starvation, carries a defective prophage called ΨM100 on its...
Hummerjohann, Jörg, Laudenbach, Sascha, Rétey, Julia, Leisinger, Thomas, Kertesz, Michael A.
A gene cluster upstream of the arylsulfatase gene (atsA) in Pseudomonas aeruginosa was characterized and found to encode a putative ABC-type transporter, AtsRBC. Mutants with insertions in the atsR...
Deletion Analysis of the Escherichia coli Taurine and Alkanesulfonate Transport Systems
Eichhorn, Eric, Van Der Ploeg, Jan R., Leisinger, Thomas
The Escherichia coli tauABCD and ssuEADCB gene clusters are required for the utilization of taurine and alkanesulfonates as sulfur sources and are expressed only under conditions of sulfate or...
The ssu Locus Plays a Key Role in Organosulfur Metabolism in Pseudomonas putida S-313
Kahnert, Antje, Vermeij, Paul, Wietek, Claudia, James, Peter, Leisinger, Thomas, Kertesz, Michael A.
Pseudomonas putida S-313 can utilize a broad range of aromatic sulfonates as sulfur sources for growth in sulfate-free minimal medium. The sulfonates are cleaved monooxygenolytically to yield the...
Metabolism of Dichloromethane by the Strict Anaerobe Dehalobacterium formicoaceticum
Mägli, Andreas, Messmer, Michael, Leisinger, Thomas
The metabolism of dichloromethane by Dehalobacterium formicoaceticum in cell suspensions and crude cell extracts was investigated. The organism is a strictly anaerobic gram-positive bacterium that...
Gisi, Daniel, Willi, Laurent, Traber, Hubert, Leisinger, Thomas, Vuilleumier, Stéphane
Methylobacterium sp. strain DM4 and Methylophilus sp. strain DM11 can grow with dichloromethane (DCM) as the sole source of carbon and energy by virtue of homologous glutathione-dependent DCM...
Chloromethane Metabolism by Methylobacterium sp. Strain CM4
Vannelli, Todd, Studer, Alex, Kertesz, Michael, Leisinger, Thomas
Methylobacterium sp. strain CM4 metabolized chloromethane quantitatively with a molar yield of 2.8 g of whole-cell protein/mol of C. This value was similar to that observed after growth with methanol...
De Azevedo Wäsch, Susana I., Van Der Ploeg, Jan R., Maire, Tere, Lebreton, Alice, Kiener, Andreas, Leisinger, Thomas
Pseudomonas sp. strain KIE171 was able to grow with isopropylamine or l-alaninol [S-(+)-2-amino-1-propanol] as the sole carbon source, but not with d-alaninol. To investigate the hypothesis that...
Studer, Alex, McAnulla, Craig, Büchele, Rainer, Leisinger, Thomas, Vuilleumier, Stéphane
Methylobacterium chloromethanicum CM4 is an aerobic α-proteobacterium capable of growth with chloromethane as the sole carbon and energy source. Two proteins, CmuA and CmuB, were previously purified...
Locher, Hans H., Thurnheer, Thomas, Leisinger, Thomas, Cook, Alasdair M.
Aerobic, carbon-limited, enrichment cultures containing 3-aminobenzenesulfonate or 3-nitrobenzenesulfonate as the sole source of carbon and energy yielded growth and complete substrate disappearance....
REPRESSION-DEPENDENT ALTERATION OF AN ARGININE ENZYME IN Escherichia coli
Leisinger, Thomas, Vogel, Ruth H., Vogel, Henry J.
Treatment of susceptible Escherichia coli K12 derivatives with 0.4 M Mg++ at 37°, potentiated by L-arginine or L-canavanine, leads to alteration of acetylornithine δ-transaminase. The alteration,...
Expression of the argA Gene Carried by a Defective Lambda Bacteriophage of Escherichia coli
Leisinger, Thomas, Haas, Dieter, Kelker, Norman
Evidence is presented that the increase in specific activity of N-acetylglutamate synthase observed upon heat induction of Escherichia coli (λdargA) is primarily due to a gene dosage effect.
Bacterial Degradation of Dichloromethane
Brunner, Walter, Staub, Doris, Leisinger, Thomas
Strain DM1, a facultative methylotrophic bacterium utilizing methanol, formate, mono-, di-, and trimethylamine, as well as dichloromethane as C1 substrates was isolated as an airborne contaminant....
N2-Succinylated intermediates in an arginine catabolic pathway of Pseudomonas aeruginosa
Jann, Alfred, Stalon, Victor, Wauven, Corinne Vander, Leisinger, Thomas, Haas, Dieter
Arginine-nonutilizing (aru) mutants of Pseudomonas aeruginosa strain PAO converted L-arginine to N2-succinylarginine or N-succinylglutamate, which were identified by high-voltage electrophoresis and...
Krishna, Rangachar V., Beilstein, Paul, Leisinger, Thomas
γ-Glutamyl phosphate reductase, the second enzyme of proline biosynthesis, catalyses the formation of l-glutamic acid 5-semialdehyde from γ-glutamyl phosphate with NAD(P)H as cofactor. It was...