Thomas Ried

Chromosomal instability determines taxane sensitivity - supplementary materials (2009)

Swanton, Charles, Nicke, Barbara, Schuett, Marion, Eklund, Aron C., Ng, Charlotte, Li, Qiyuan, ...

Microtubule-stabilizing (MTS) agents, such as taxanes, are important chemotherapeutics with a poorly understood mechanism of action. We identified a set of genes repressed in multiple cell lines in...

Chromosome Transfer Induced Aneuploidy Results in Complex Dysregulation of the Cellular Transcriptome in Immortalized and Cancer Cells (2008)

Madhvi B. Upender, Jens K. Habermann, Lisa M. Mcshane, Edward L. Korn, J. Carl Barrett, Thomas Ried

Chromosomal aneuploidies are observed in essentially all sporadic carcinomas. These aneuploidies result in tumor-specific patterns of genomic imbalances that are acquired early during tumorigenesis,...

A sequence-based survey of the complex structural organization of tumor genomes (2008)

Raphael, Benjamin J, Volik, Stanislav, Yu, Peng, Wu, Chunxiao, Huang, Guiqing, Linardopoulou, Elena V, ...

Abstract Background The genomes of many epithelial tumors exhibit extensive chromosomal rearrangements. All classes of genome rearrangements can be identified using end sequencing profiling, which...

RAPID COMMUNICATION Frequent Dysregulation of the c-maf Proto-Oncogene at 16q23 by Translocation to an Ig Locus in Multiple Myeloma (2007)

Marta Chesi, P. Leif Bergsagel, Oluwatoyin O. Shonukan, Maria Luisa Martelli, Leslie A. Brents, Theresa Chen, ...

Dysregulation of oncogenes by translocation to an IgH (14q32) or IgL (�, 2p11 or �, 22q11) locus is a frequent event in the pathogenesis of B-cell tumors. Translocations involving an IgH locus...

Actin-dependent intranuclear repositioning of an active gene locus in vivo. (2007)

Dundr, Miroslav, Ospina, Jason K., Sung, Myong-Hee, John, Sam, Upender, Madhvi, Ried, Thomas, ...

Although bulk chromatin is thought to have limited mobility within the interphase eukaryotic nucleus, directed long-distance chromosome movements are not unknown. Cajal bodies (CBs) are nuclear...

Molecular and cytological features of the mouse B-cell lymphoma line iMycEμ-1 (2005)

Su Han, Seong, Shaffer, Arthur L, Peng, Liangping, Chung, Seung, Lim, Jae, Maeng, Sungho, ...

Abstract Background Myc -induced lymphoblastic B-cell lymphoma (LBL) in iMyc Eμ mice may provide a model system for the study of the mechanism by which human MYC facilitates the initiation and...

SMC1 involvement in fragile site expression (2005)

Musio, Antonio, Montagna, Cristina, Mariani, Tullio, Tilenni, Manuela, Focarelli, Maria Luisa, Brait, Lorenzo, ...

Common fragile sites have been involved in neoplastic transformation, although their molecular basis is still poorly understood. Here, we demonstrate that inhibition of the SMC1 by RNAi is sufficient...

Multicolor fluorescence in situ hybridization on metaphase chromosomes and interphase Halo-preparations using cosmid and YAC clones for the simultaneous high resolution mapping of deletions in the dystrophin gene (1994)

Tocharoentanaphol, Chintana, Cremer, Marion, Schröck, Evelin, Blonden, Lau, Kilian, Karin, Cremer, Thomas, ...

We report on multicolor fluorescence in situ hybridization protocols for the simultaneous visualization of deletion-prone regions for carrier detection of Duchenne/ Becker (DMD/BMD) muscular...

Mapping of Multiple DNA Gains and Losses in Primary Small Cell Lung Carcinomas by Comparative Genomic Hybridization (1994)

Ried, Thomas, Petersen, Iver, Holtgreve-Grez, Heidi, Speicher, Michael R., Schröck, Evelin, Manoir, Stanislas Du, ...

Comparative genomic hybridization was applied for a comprehensive screening of under- and overrepresentation of genetic material in 13 autoptic small cell lung cancer specimens. The most abundant...

Comparative Genomic Hybridization of Human Malignant Gliomas Reveals Multiple Amplification Sites and Nonrandom Chromosomal Gains and Losses (1994)

Schröck, Evelin, Thiel, G., Lozonova, T., Manoir, Stanislas Du, Meffert, M. C., Jauch, Anna, ...

Nine human malignant gliomas (2 astrocytomas grade III and 7 glioblastomas) were analyzed using comparative genomic hybridization (CGH). In addition to the amplification of the EGFR gene at 7p12 in 4...

Specific loss of chromosomes 1, 2, 6, 10, 13, 17, and 21 in chromophobe renal cell carcinomas revealed by comparative genomic hybridization (1994)

Speicher, Michael R., Schoell, B., Manoir, Stanislas Du, Schröck, Evelin, Ried, Thomas, Cremer, Thomas, ...

We analyzed 19 chromophobe renal cell carcinomas by means of comparative genomic hybridization. Two tumors revealed no numerical abnormalities. In the remaining 17 cases we found loss of entire...

Molecular cytogenetic analysis of formalin-fixed, paraffin-embedded solid tumors by comparative genomic hybridization after universal DNA-amplification (1993)

Speicher, Michael R., Manoir, Stanislas Du, Schröck, Evelin, Holtgreve-Grez, Heidi, Schoell, B., Lengauer, Christoph, ...

We present a technique which allows the detection and chromosomal localization of DNA sequence copy number changes in solid tumor genomes from frozen sections and paraffin embedded, formalin fixed...

Molecular cytogenetic analysis of formalin-fixed, paraffin-embedded solid tumors by comparative genomic hybridization after universal DNA-amplification (1993)

Spelcher, Michael R., Manoir, Stanislas Du, SchrÖck, Evelln, Holtgreve-Grez, Heidi, Schoell, Brlgltte, Lengauer, Christoph, ...

We present a technique which allows the detection and chromosomal localization of DNA sequence copy number changes in solid tumor genomes from frozen sections and paraffin embedded, formalin fixed...

Clustered organization of homologous KRAB zinc-finger genes with enhanced expression in human T lymphoid cells (1993)

Bellefroid, Eric J, Marine, Jean-Christophe, Ried, Thomas, Lecocq, Pierre J, Riviere, Michele, Amemiya, Chris, ...

KRAB zinc-finger proteins (KRAB-ZFPs) constitute a large subfamily of ZFPs of the Krüppel C2H2 type. KRAB (Krüppel-associated box) is an evolutionarily conserved protein domain found N-terminally...

Specific metaphase and interphase detection of the breakpoint region in 8q24 of burkitt lymphoma cells by triple-color fluorescence in situ hybridization (1992)

Ried, Thomas, Lengauer, Christoph, Cremer, Thomas, Wiegant, Joop, Raap, Anton K., Van Der Ploeg, Mels, ...

Triple fluorescence in situ hybridization with a plasmid DNA library from sorted human chromosomes 8 in combination with bacteriophage clones flanking the breakpoint in 8q24 of the Burkitt lymphoma...

Localization of the human KRAB finger gene ZNF117 (HPF9) to chromosome 7q11.2 (1992)

Bellefroid, Eric J, Ried, Thomas, Riviere, Michele, Marine, Jean-Christophe, Levan, G, Szpirer, J, ...

A cluster of Kruppel type zinc finger genes of the KRAB subclass has recently been localized on human chromosome 19p12-p13.1. We now report that ZNF117 (HPF9), a closely related zinc finger gene of...

Multicolor fluorescence in situ hybridization for the simultaneous detection of probe sets for chromosomes 13, 18, 21, X and Y in uncultured amniotic fluid cells (1992)

Ried, Thomas, Landes, Greg, Dackowski, William, Klinger, Katherine, Ward, David C.

The most frequent aneuploidies in newborns involve the autosomes 13, 18 and 21 as well as both sex chromosomes. Fluorescence in situ hybridization readily allows the detection of numerical...

In situ hybridisation with fluoresceinated DNA (1991)

Wiegant, Joop, Ried, Thomas, Nederlof, Petra M., Ploeg, Mels Van Der, Tanke, Hans J., Raap, Anton K.

We have used fluorescein-11-dUTP in a nick-translation format to produce fluoresceinated human nucleic acid probes. After In situ hybridization of fluoresceinated DNAs to human metaphase chromosomes,...

Direct carrier detection by in situ suppression hybridization with cosmid clones of the Duchenne/Becker muscular dystrophy locus (1990)

Ried, Thomas, Mahler, V., Vogt, Peter, Blonden, L., Cremer, Thomas

A basic problem in genetic counseling of families with Duchenne/Becker muscular dystrophy (DMD/BMD) concerns the carrier status of female relatives of an affected male. In about 60% of these...

Recombinase-activating gene (RAG) 2-mediated V(D)J recombination is not essential for tumorigenesis in Atm-deficient mice

Petiniot, Lisa K., Weaver, Zoë, Barlow, Carrolee, Shen, Rhuna, Eckhaus, Michael, Steinberg, Seth M., ...

The majority of Atm-deficient mice die of malignant thymic lymphoma by 4–5 mo of age. Cytogenetic abnormalities in these tumors are consistently identified within the Tcr α/δ locus, suggesting...

SHOT, a SHOX-related homeobox gene, is implicated in craniofacial, brain, heart, and limb development

Blaschke, Rüdiger J., Monaghan, A. Paula, Schiller, Simone, Schechinger, Birgit, Rao, Ercole, Padilla-Nash, Hesed, ...

Deletion of the SHOX region on the human sex chromosomes has been shown to result in idiopathic short stature and proposed to play a role in the short stature associated with Turner syndrome. We have...

Chromosomal aberrations in PARP−/− mice: Genome stabilization in immortalized cells by reintroduction of poly(ADP-ribose) polymerase cDNA

Simbulan-Rosenthal, Cynthia M., Haddad, Bassem R., Rosenthal, Dean S., Weaver, Zoë, Coleman, Allen, Luo, RuiBai, ...

Depletion of poly(ADP-ribose) polymerase (PARP) increases the frequency of recombination, gene amplification, sister chromatid exchanges, and micronuclei formation in cells exposed to genotoxic...

RAG-Mediated V(D)J Recombination Is Not Essential for Tumorigenesis in Atm-Deficient Mice

Petiniot, Lisa K., Weaver, Zoë, Vacchio, Melanie, Shen, Rhuna, Wangsa, Danny, Barlow, Carrolee, ...

Atm-deficient mice die of malignant thymic lymphomas characterized by translocations within the Tcrα/δ locus, suggesting that tumorigenesis is secondary to aberrant responses to double-stranded DNA...

Involvement of Crm1 in Hepatitis B Virus X Protein-Induced Aberrant Centriole Replication and Abnormal Mitotic Spindles

Forgues, Marshonna, Difilippantonio, Michael J., Linke, Steven P., Ried, Thomas, Nagashima, Kunio, Feden, Jeffrey, ...

Hepatitis B virus (HBV) includes an X gene (HBx gene) that plays a critical role in liver carcinogenesis. Because centrosome abnormalities are associated with genomic instability in most human cancer...

Of Mice and MEN1: Insulinomas in a Conditional Mouse Knockout

Crabtree, Judy S., Scacheri, Peter C., Ward, Jerrold M., McNally, Sara R., Swain, Gary P., Montagna, Cristina, ...

Patients with multiple endocrine neoplasia type 1 (MEN1) develop multiple endocrine tumors, primarily affecting the parathyroid, pituitary, and endocrine pancreas, due to the inactivation of the MEN1...

Haploinsufficiency of Anx7 tumor suppressor gene and consequent genomic instability promotes tumorigenesis in the Anx7(+/-) mouse

Srivastava, Meera, Montagna, Cristina, Leighton, Ximena, Glasman, Mirta, Naga, Shanmugam, Eidelman, Ofer, ...

Annexin 7 (ANX7) acts as a tumor suppressor gene in prostate cancer, where loss of heterozygosity and reduction of ANX7 protein expression is associated with aggressive metastatic tumors. To...

The Interactive Online SKY/M-FISH & CGH Database and the Entrez Cancer Chromosomes Search Database: Linkage of Chromosomal Aberrations with the Genome Sequence

Knutsen, Turid, Gobu, Vasuki, Knaus, Rodger, Padilla-Nash, Hesed, Augustus, Meena, Strausberg, Robert L., ...

To catalogue data on chromosomal aberrations in cancer derived from emerging molecular cytogenetic techniques and to integrate these data with genome maps, we have established two resources, the NCI...

53BP1 Cooperates with p53 and Functions as a Haploinsufficient Tumor Suppressor in Mice

Ward, Irene M., Difilippantonio, Simone, Minn, Kay, Mueller, Melissa D., Molina, Julian R., Yu, Xiaochun, ...

p53 binding protein 1 (53BP1) is a putative DNA damage sensor that accumulates at sites of double-strand breaks (DSBs) in a manner dependent on histone H2AX. Here we show that the loss of one or both...

A comprehensive continuous-time model for the appearance of CGH signal due to chromosomal missegregations during mitosis

Desper, Richard, Difilippantonio, Michael J., Ried, Thomas, Schäffer, Alejandro A.

Aneuploidy, the gain or loss of large regions of the genome, is a common feature in cancer cells. Irregularities in chromosomal copy number caused by missegregations of chromosomes during mitosis can...

Ataxia telangiectasia mutated is essential during adult neurogenesis

Allen, Duane M., Van Praag, Henriette, Ray, Jasodhara, Weaver, Zoë, Winrow, Christopher J., Carter, Todd A., ...

Ataxia telangiectasia (A-T) is an autosomal recessive disease characterized by normal brain development followed by progressive neurodegeneration. The gene mutated in A-T (ATM) is a serine protein...

Recombinase-activating gene (RAG) 2-mediated V(D)J recombination is not essential for tumorigenesis in Atm-deficient mice

Petiniot, Lisa K., Weaver, Zoë, Barlow, Carrolee, Shen, Rhuna, Eckhaus, Michael, Steinberg, Seth M., ...

The majority of Atm-deficient mice die of malignant thymic lymphoma by 4–5 mo of age. Cytogenetic abnormalities in these tumors are consistently identified within the Tcr α/δ locus, suggesting...

SHOT, a SHOX-related homeobox gene, is implicated in craniofacial, brain, heart, and limb development

Blaschke, Rüdiger J., Monaghan, A. Paula, Schiller, Simone, Schechinger, Birgit, Rao, Ercole, Padilla-Nash, Hesed, ...

Deletion of the SHOX region on the human sex chromosomes has been shown to result in idiopathic short stature and proposed to play a role in the short stature associated with Turner syndrome. We have...

Chromosomal aberrations in PARP−/− mice: Genome stabilization in immortalized cells by reintroduction of poly(ADP-ribose) polymerase cDNA

Simbulan-Rosenthal, Cynthia M., Haddad, Bassem R., Rosenthal, Dean S., Weaver, Zoë, Coleman, Allen, Luo, RuiBai, ...

Depletion of poly(ADP-ribose) polymerase (PARP) increases the frequency of recombination, gene amplification, sister chromatid exchanges, and micronuclei formation in cells exposed to genotoxic...

RAG-Mediated V(D)J Recombination Is Not Essential for Tumorigenesis in Atm-Deficient Mice

Petiniot, Lisa K., Weaver, Zoë, Vacchio, Melanie, Shen, Rhuna, Wangsa, Danny, Barlow, Carrolee, ...

Atm-deficient mice die of malignant thymic lymphomas characterized by translocations within the Tcrα/δ locus, suggesting that tumorigenesis is secondary to aberrant responses to double-stranded DNA...

Involvement of Crm1 in Hepatitis B Virus X Protein-Induced Aberrant Centriole Replication and Abnormal Mitotic Spindles

Forgues, Marshonna, Difilippantonio, Michael J., Linke, Steven P., Ried, Thomas, Nagashima, Kunio, Feden, Jeffrey, ...

Hepatitis B virus (HBV) includes an X gene (HBx gene) that plays a critical role in liver carcinogenesis. Because centrosome abnormalities are associated with genomic instability in most human cancer...

Of Mice and MEN1: Insulinomas in a Conditional Mouse Knockout

Crabtree, Judy S., Scacheri, Peter C., Ward, Jerrold M., McNally, Sara R., Swain, Gary P., Montagna, Cristina, ...

Patients with multiple endocrine neoplasia type 1 (MEN1) develop multiple endocrine tumors, primarily affecting the parathyroid, pituitary, and endocrine pancreas, due to the inactivation of the MEN1...

Haploinsufficiency of Anx7 tumor suppressor gene and consequent genomic instability promotes tumorigenesis in the Anx7(+/-) mouse

Srivastava, Meera, Montagna, Cristina, Leighton, Ximena, Glasman, Mirta, Naga, Shanmugam, Eidelman, Ofer, ...

Annexin 7 (ANX7) acts as a tumor suppressor gene in prostate cancer, where loss of heterozygosity and reduction of ANX7 protein expression is associated with aggressive metastatic tumors. To...

Ataxia telangiectasia mutated is essential during adult neurogenesis

Allen, Duane M., Van Praag, Henriette, Ray, Jasodhara, Weaver, Zoë, Winrow, Christopher J., Carter, Todd A., ...

Ataxia telangiectasia (A-T) is an autosomal recessive disease characterized by normal brain development followed by progressive neurodegeneration. The gene mutated in A-T (ATM) is a serine protein...

53BP1 Cooperates with p53 and Functions as a Haploinsufficient Tumor Suppressor in Mice

Ward, Irene M., Difilippantonio, Simone, Minn, Kay, Mueller, Melissa D., Molina, Julian R., Yu, Xiaochun, ...

p53 binding protein 1 (53BP1) is a putative DNA damage sensor that accumulates at sites of double-strand breaks (DSBs) in a manner dependent on histone H2AX. Here we show that the loss of one or both...

Genomic Amplification of the Human Telomerase Gene (TERC) in Pap Smears Predicts the Development of Cervical Cancer

Heselmeyer-Haddad, Kerstin, Sommerfeld, Kathrin, White, Nicole M., Chaudhri, Nadia, Morrison, Larry E., Palanisamy, Nallasivam, ...

Invasive cervical carcinomas almost invariably carry extra copies of chromosome arm 3q, resulting in a gain of the human telomerase gene (TERC). This provided the rationale for the development of a...

Specific Chromosomal Aberrations and Amplification of the AIB1 Nuclear Receptor Coactivator Gene in Pancreatic Carcinomas

Ghadimi, B. Michael, Schröck, Evelin, Walker, Robert L., Wangsa, Danny, Jauho, Annukka, Meltzer, Paul S., ...

To screen pancreatic carcinomas for chromosomal aberrations we have applied molecular cytogenetic techniques, including fluorescent in situ hybridization, comparative genomic hybridization, and...

Detection of Genomic Amplification of the Human Telomerase Gene (TERC) in Cytologic Specimens as a Genetic Test for the Diagnosis of Cervical Dysplasia

Heselmeyer-Haddad, Kerstin, Janz, Viktor, Castle, Philip E., Chaudhri, Nadia, White, Nicole, Wilber, Kim, ...

Invasive cervical carcinomas frequently reveal additional copies of the long arm of chromosome 3. The detection of this genetic aberration in diagnostic samples could therefore complement the...

Comparative Genomic Hybridization of Human Malignant Gliomas Reveals Multiple Amplification Sites and Nonrandom Chromosomal Gains and Losses

Schròck, Evelin, Thiel, Gundula, Lozanova, Tanka, Du Manoir, Stanislas, Meffert, Marie-Christine, Jauch, Anna, ...

Nine human malignant gliomas (2 astrocytomas grade III and 7 glioblastomas) were analyzed using comparative genomic hybridization (CGH). In addition to the amplification of the EGFR gene at 7p12 in 4...

Evidence for Replicative Repair of DNA Double-Strand Breaks Leading to Oncogenic Translocation and Gene Amplification

Difilippantonio, Michael J., Petersen, Simone, Chen, Hua Tang, Johnson, Roger, Jasin, Maria, Kanaar, Roland, ...

Nonreciprocal translocations and gene amplifications are commonly found in human tumors. Although little is known about the mechanisms leading to such aberrations, tissue culture models predict that...

AID-deficient Bcl-xL transgenic mice develop delayed atypical plasma cell tumors with unusual Ig/Myc chromosomal rearrangements

Kovalchuk, Alexander L., DuBois, Wendy, Mushinski, Elizabeth, McNeil, Nicole E., Hirt, Carsten, Qi, Chen-Feng, ...

Activation-induced cytidine deaminase (AID) is required for immunoglobulin (Ig) class switch recombination and somatic hypermutation, and has also been implicated in translocations between Ig switch...

A sequence-based survey of the complex structural organization of tumor genomes

Raphael, Benjamin J, Volik, Stanislav, Yu, Peng, Wu, Chunxiao, Huang, Guiqing, Linardopoulou, Elena V, ...

Tumors and cancer cell lines were surveyed with end-sequencing profiling, yielding the largest available collection of sequence-ready tumor genome breakpoints and providing evidence that some...

Cytokine-independent growth and clonal expansion of a primary human CD8+ T-cell clone following retroviral transduction with the IL-15 gene

Hsu, Cary, Jones, Stephanie A., Cohen, Cyrille J., Zheng, Zhili, Kerstann, Keith, Zhou, Juhua, ...

Malignancies arising from retrovirally transduced hematopoietic stem cells have been reported in animal models and human gene therapy trials. Whether mature lymphocytes are susceptible to insertional...

Actin-dependent intranuclear repositioning of an active gene locus in vivo

Dundr, Miroslav, Ospina, Jason K., Sung, Myong-Hee, John, Sam, Upender, Madhvi, Ried, Thomas, ...

Although bulk chromatin is thought to have limited mobility within the interphase eukaryotic nucleus, directed long-distance chromosome movements are not unknown. Cajal bodies (CBs) are nuclear...

β-Catenin Expression Results in p53-Independent DNA Damage and Oncogene-Induced Senescence in Prelymphomagenic Thymocytes In Vivo▿ †

Xu, Mai, Yu, Qing, Subrahmanyam, Ramesh, Difilippantonio, Michael J., Ried, Thomas, Sen, Jyoti Misra

The expression of β-catenin, a potent oncogene, is causally linked to tumorigenesis. Therefore, it was surprising that the transgenic expression of oncogenic β-catenin in thymocytes resulted in...

A genomic approach to colon cancer risk stratification yields biologic insights into therapeutic opportunities

Garman, Katherine S., Acharya, Chaitanya R., Edelman, Elena, Grade, Marian, Gaedcke, Jochen, Sud, Shivani, ...

Gene expression profiles provide an opportunity to dissect the heterogeneity of solid tumors, including colon cancer, to improve prognosis and predict response to therapies. Bayesian binary...