Chan, Camie W, Crafton, Emily, Fan, Hong-Ni, Flook, James, Yoshimura, Kiyoshi, Skarica, Mario, ...
Natural killer (NK) cells and dendritic cells (DCs) are, respectively, central components of innate and adaptive immune responses(1,2). We describe here a third DC lineage, termed...
Stable alphavirus packaging cell lines for Sindbis virus- and Semliki Forest virus-derived vectors
Polo, John M., Belli, Barbara A., Driver, David A., Frolov, Ilya, Sherrill, Scott, Hariharan, Mangala J., ...
Alphavirus vectors are being developed for possible human vaccine and gene therapy applications. We have sought to advance this field by devising DNA-based vectors and approaches for the production...
Perri, Silvia, Driver, David A., Gardner, Jason P., Sherrill, Scott, Belli, Barbara A., Dubensky, Thomas W., ...
Alphavirus replicon vectors are well suited for applications where transient, high-level expression of a heterologous gene is required. Replicon vector expression in cells leads to inhibition of host...
Gardner, Jason P., Frolov, Ilya, Perri, Silvia, Ji, Yaying, MacKichan, Mary Lee, Zur Megede, Jan, ...
The ability to target antigen-presenting cells with vectors encoding desired antigens holds the promise of potent prophylactic and therapeutic vaccines for infectious diseases and cancer. Toward this...
DePolo, Nicholas J., Harkleroad, Cataline E., Bodner, Mordechai, Watt, Andrew T., Anderson, Carol G., Greengard, Judith S., ...
The ability to deliver genes as therapeutics requires an understanding of the vector pharmacokinetics similar to that required for conventional drugs. A first question is the half-life of the vector...
DNA Immunization against Herpes Simplex Virus: Enhanced Efficacy Using a Sindbis Virus-Based Vector
Hariharan, Mangala J., Driver, David A., Townsend, Kay, Brumm, Duane, Polo, John M., Belli, Barbara A., ...
Previously we reported the development of a plasmid DNA expression vector system derived from Sindbis virus (T. W. Dubensky, Jr., et al., J. Virol. 70:508–519, 1996). In vitro, such vectors exhibit...
Perri, Silvia, Greer, Catherine E., Thudium, Kent, Doe, Barbara, Legg, Harold, Liu, Hong, ...
Alphavirus replicon particle-based vaccine vectors derived from Sindbis virus (SIN), Semliki Forest virus, and Venezuelan equine encephalitis virus (VEE) have been shown to induce robust...
Wang, Guoshun, Slepushkin, Vladimir, Zabner, Joseph, Keshavjee, Shaf, Johnston, Julie C., Sauter, Sybille L., ...
Several problems limit the application of gene transfer to correct the cystic fibrosis (CF) Cl– transport defect in airway epithelia. These include inefficient transduction with vectors applied to...
Listeria-based cancer vaccines that segregate immunogenicity from toxicity
Brockstedt, Dirk G., Giedlin, Martin A., Leong, Meredith L., Bahjat, Keith S., Gao, Yi, Luckett, William, ...
The facultative intracellular bacterium Listeria monocytogenes is being developed as a cancer vaccine platform because of its ability to induce potent innate and adaptive immunity. For successful...
Stable alphavirus packaging cell lines for Sindbis virus- and Semliki Forest virus-derived vectors
Polo, John M., Belli, Barbara A., Driver, David A., Frolov, Ilya, Sherrill, Scott, Hariharan, Mangala J., ...
Alphavirus vectors are being developed for possible human vaccine and gene therapy applications. We have sought to advance this field by devising DNA-based vectors and approaches for the production...
Perri, Silvia, Driver, David A., Gardner, Jason P., Sherrill, Scott, Belli, Barbara A., Dubensky, Thomas W., ...
Alphavirus replicon vectors are well suited for applications where transient, high-level expression of a heterologous gene is required. Replicon vector expression in cells leads to inhibition of host...
Gardner, Jason P., Frolov, Ilya, Perri, Silvia, Ji, Yaying, MacKichan, Mary Lee, Zur Megede, Jan, ...
The ability to target antigen-presenting cells with vectors encoding desired antigens holds the promise of potent prophylactic and therapeutic vaccines for infectious diseases and cancer. Toward this...
DePolo, Nicholas J., Harkleroad, Cataline E., Bodner, Mordechai, Watt, Andrew T., Anderson, Carol G., Greengard, Judith S., ...
The ability to deliver genes as therapeutics requires an understanding of the vector pharmacokinetics similar to that required for conventional drugs. A first question is the half-life of the vector...
DNA Immunization against Herpes Simplex Virus: Enhanced Efficacy Using a Sindbis Virus-Based Vector
Hariharan, Mangala J., Driver, David A., Townsend, Kay, Brumm, Duane, Polo, John M., Belli, Barbara A., ...
Previously we reported the development of a plasmid DNA expression vector system derived from Sindbis virus (T. W. Dubensky, Jr., et al., J. Virol. 70:508–519, 1996). In vitro, such vectors exhibit...
Perri, Silvia, Greer, Catherine E., Thudium, Kent, Doe, Barbara, Legg, Harold, Liu, Hong, ...
Alphavirus replicon particle-based vaccine vectors derived from Sindbis virus (SIN), Semliki Forest virus, and Venezuelan equine encephalitis virus (VEE) have been shown to induce robust...
Wang, Guoshun, Slepushkin, Vladimir, Zabner, Joseph, Keshavjee, Shaf, Johnston, Julie C., Sauter, Sybille L., ...
Several problems limit the application of gene transfer to correct the cystic fibrosis (CF) Cl– transport defect in airway epithelia. These include inefficient transduction with vectors applied to...
Listeria-based cancer vaccines that segregate immunogenicity from toxicity
Brockstedt, Dirk G., Giedlin, Martin A., Leong, Meredith L., Bahjat, Keith S., Gao, Yi, Luckett, William, ...
The facultative intracellular bacterium Listeria monocytogenes is being developed as a cancer vaccine platform because of its ability to induce potent innate and adaptive immunity. For successful...
Cytosolic Entry Controls CD8+-T-Cell Potency during Bacterial Infection▿
Bahjat, Keith S., Liu, Weiqun, Lemmens, Edward E., Schoenberger, Stephen P., Portnoy, Daniel A., Dubensky, Thomas W., ...
Interaction with host immunoreceptors during microbial infection directly impacts the magnitude of the ensuing innate immune response. How these signals affect the quality of the adaptive T-cell...
Role of PD-1 and its ligand, B7-H1, in early fate decisions of CD8 T cells
Goldberg, Monica V., Maris, Charles H., Hipkiss, Edward L., Flies, Andrew S., Zhen, Lijie, Tuder, Rubin M., ...
Expression of the PD-1 receptor on T cells has been shown to provide an important inhibitory signal that down-modulates peripheral effector responses in normal tissues and tumors. Furthermore, PD-1...
Lauer, Peter, Hanson, Bill, Lemmens, Edward E., Liu, Weiqun, Luckett, William S., Leong, Meredith L., ...
Recombinant vaccines derived from the facultative intracellular bacterium Listeria monocytogenes are presently undergoing early-stage clinical evaluation in oncology treatment settings. This effort...
KBMA Listeria monocytogenes is an effective vector for DC-mediated induction of antitumor immunity
Skoberne, Mojca, Yewdall, Alice, Bahjat, Keith S., Godefroy, Emmanuelle, Lauer, Peter, Lemmens, Edward, ...
Vaccine strategies that utilize human DCs to enhance antitumor immunity have yet to realize their full potential. Approaches that optimally target a spectrum of antigens to DCs are urgently needed....
Crimmins, Gregory T., Herskovits, Anat A., Rehder, Kai, Sivick, Kelsey E., Lauer, Peter, Dubensky, Thomas W., ...
To gain insight into the interaction of intracellular pathogens with host innate immune pathways, we performed an unbiased genetic screen of Listeria monocytogenes mutants that induced an enhanced or...
Suppression of Cell-Mediated Immunity following Recognition of Phagosome-Confined Bacteria
Bahjat, Keith S., Meyer-Morse, Nicole, Lemmens, Edward E., Shugart, Jessica A., Dubensky, Thomas W., Brockstedt, Dirk G., ...
Listeria monocytogenes is a facultative intracellular pathogen capable of inducing a robust cell-mediated immune response to sub-lethal infection. The capacity of L. monocytogenes to escape from the...
Skoble, Justin, Beaber, John W., Gao, Yi, Lovchik, Julie A., Sower, Laurie E., Liu, Weiqun, ...
Bacillus anthracis is the causative agent of anthrax. We have developed a novel whole-bacterial-cell anthrax vaccine utilizing B. anthracis that is killed but metabolically active (KBMA). Vaccine...