Variation in bacterial flagellins: from sequence to structure (2006)
Scott A. Beatson, Tohru Minamino, Mark J. Pallen
We chose to undertake a comprehensive analysis of all available bacteral flagellin sequences in light of the 3D structure of Salmonella typhimurium flagellin. Remarkable conservation in the regions...
Variation in bacterial flagellins: from sequence to structure (2006)
Beatson, Scott A., Minamino, Tohru, Pallen, Mark J.
Bacterial motility relies chiefly on the rotation of a molecular propeller, the flagellar filament, which is constructed from the protein flagellin. Here, flagellin sequence conservation and...
Variation in bacterial flagellins: from sequence to structure (2006)
Beatson, Scott A., Minamino, Tohru, Pallen, Mark J.
Bacterial motility relies chiefly on the rotation of a molecular propeller, the flagellar filament, which is constructed from the protein flagellin. Here, flagellin sequence conservation and...
Minamino, Tohru, Christou, Helen, Hsieh, Chung-Ming, Liu, Yuxiang, Dhawan, Vijender, Abraham, Nader G., ...
Chronic hypoxia causes pulmonary hypertension with smooth muscle cell proliferation and matrix deposition in the wall of the pulmonary arterioles. We demonstrate here that hypoxia also induces a...
Components of the Salmonella Flagellar Export Apparatus and Classification of Export Substrates
Minamino, Tohru, Macnab, Robert M.
Until now, identification of components of the flagellar protein export apparatus has been indirect. We have now identified these components directly by establishing whether mutants defective in...
Nambu, Takayuki, Minamino, Tohru, Macnab, Robert M., Kutsukake, Kazuhiro
Because the rod structure of the flagellar basal body crosses the inner membrane, the periplasmic space, and the outer membrane, its formation must involve hydrolysis of the peptidoglycan layer. So...
Minamino, Tohru, Yamaguchi, Shigeru, Macnab, Robert M.
FliE is a flagellar basal body protein of Salmonella whose detailed location and function have not been established. A mutant allele of fliE, which caused extremely poor flagellation and swarming,...
Kihara, May, Minamino, Tohru, Yamaguchi, Shigeru, Macnab, Robert M.
The MS ring of the flagellar basal body of Salmonella is an integral membrane structure consisting of about 26 subunits of a 61-kDa protein, FliF. Out of many nonflagellate fliF mutants tested, three...
Role of FliJ in Flagellar Protein Export in Salmonella
Minamino, Tohru, Chu, Ryan, Yamaguchi, Shigeru, Macnab, Robert M.
We isolated and characterized spontaneous mutants with defects in the 147-amino-acid Salmonella protein FliJ, which is a cytoplasmic component of the type III flagellar export apparatus. These...
Minamino, Tohru, Macnab, Robert M.
We have investigated the properties of the cytoplasmic domain (FlhBC) of the 383-amino-acid Salmonella membrane protein FlhB, a component of the type III flagellar export apparatus. FlhB, along with...
Effect of Intracellular pH on Rotational Speed of Bacterial Flagellar Motors†
Minamino, Tohru, Imae, Yasuo, Oosawa, Fumio, Kobayashi, Yuji, Oosawa, Kenji
Weak acids such as acetate and benzoate, which partially collapse the transmembrane proton gradient, not only mediate pH taxis but also impair the motility of Escherichia coli and Salmonella at an...
Substrate Specificity Classes and the Recognition Signal for Salmonella Type III Flagellar Export
Hirano, Takanori, Minamino, Tohru, Namba, Keiichi, Macnab, Robert M.
Most flagellar proteins of Salmonella are exported to their assembly destination via a specialized apparatus. This apparatus is a member of the type III superfamily, which is widely used for...
Minamino, Tohru, González-Pedrajo, Bertha, Kihara, May, Namba, Keiichi, Macnab, Robert M.
Salmonella FliI is the ATPase that drives flagellar protein export. It normally exists as a complex together with the regulatory protein FliH. A fliH null mutant was slightly motile, with...
Miyauchi, Hideyuki, Minamino, Tohru, Tateno, Kaoru, Kunieda, Takeshige, Toko, Haruhiro, Komuro, Issei
The signaling pathway of insulin/insulin-like growth factor-1/phosphatidylinositol-3 kinase/Akt is known to regulate longevity as well as resistance to oxidative stress in the nematode Caenorhabditis...
Regeneration of the endothelium as a novel therapeutic strategy for acute lung injury
Minamino, Tohru, Komuro, Issei
Acute lung injury (ALI) is characterized by the influx of protein-rich edematous fluid into the airspaces due to increased permeability of the alveolar-capillary barrier. Inflammatory mediators are...
Minamino, Tohru, Christou, Helen, Hsieh, Chung-Ming, Liu, Yuxiang, Dhawan, Vijender, Abraham, Nader G., ...
Chronic hypoxia causes pulmonary hypertension with smooth muscle cell proliferation and matrix deposition in the wall of the pulmonary arterioles. We demonstrate here that hypoxia also induces a...
Components of the Salmonella Flagellar Export Apparatus and Classification of Export Substrates
Minamino, Tohru, Macnab, Robert M.
Until now, identification of components of the flagellar protein export apparatus has been indirect. We have now identified these components directly by establishing whether mutants defective in...
Nambu, Takayuki, Minamino, Tohru, Macnab, Robert M., Kutsukake, Kazuhiro
Because the rod structure of the flagellar basal body crosses the inner membrane, the periplasmic space, and the outer membrane, its formation must involve hydrolysis of the peptidoglycan layer. So...
Minamino, Tohru, Yamaguchi, Shigeru, Macnab, Robert M.
FliE is a flagellar basal body protein of Salmonella whose detailed location and function have not been established. A mutant allele of fliE, which caused extremely poor flagellation and swarming,...
Kihara, May, Minamino, Tohru, Yamaguchi, Shigeru, Macnab, Robert M.
The MS ring of the flagellar basal body of Salmonella is an integral membrane structure consisting of about 26 subunits of a 61-kDa protein, FliF. Out of many nonflagellate fliF mutants tested, three...
Role of FliJ in Flagellar Protein Export in Salmonella
Minamino, Tohru, Chu, Ryan, Yamaguchi, Shigeru, Macnab, Robert M.
We isolated and characterized spontaneous mutants with defects in the 147-amino-acid Salmonella protein FliJ, which is a cytoplasmic component of the type III flagellar export apparatus. These...
Minamino, Tohru, Macnab, Robert M.
We have investigated the properties of the cytoplasmic domain (FlhBC) of the 383-amino-acid Salmonella membrane protein FlhB, a component of the type III flagellar export apparatus. FlhB, along with...
Effect of Intracellular pH on Rotational Speed of Bacterial Flagellar Motors†
Minamino, Tohru, Imae, Yasuo, Oosawa, Fumio, Kobayashi, Yuji, Oosawa, Kenji
Weak acids such as acetate and benzoate, which partially collapse the transmembrane proton gradient, not only mediate pH taxis but also impair the motility of Escherichia coli and Salmonella at an...
Substrate Specificity Classes and the Recognition Signal for Salmonella Type III Flagellar Export
Hirano, Takanori, Minamino, Tohru, Namba, Keiichi, Macnab, Robert M.
Most flagellar proteins of Salmonella are exported to their assembly destination via a specialized apparatus. This apparatus is a member of the type III superfamily, which is widely used for...
Minamino, Tohru, González-Pedrajo, Bertha, Kihara, May, Namba, Keiichi, Macnab, Robert M.
Salmonella FliI is the ATPase that drives flagellar protein export. It normally exists as a complex together with the regulatory protein FliH. A fliH null mutant was slightly motile, with...
Miyauchi, Hideyuki, Minamino, Tohru, Tateno, Kaoru, Kunieda, Takeshige, Toko, Haruhiro, Komuro, Issei
The signaling pathway of insulin/insulin-like growth factor-1/phosphatidylinositol-3 kinase/Akt is known to regulate longevity as well as resistance to oxidative stress in the nematode Caenorhabditis...
Regeneration of the endothelium as a novel therapeutic strategy for acute lung injury
Minamino, Tohru, Komuro, Issei
Acute lung injury (ALI) is characterized by the influx of protein-rich edematous fluid into the airspaces due to increased permeability of the alveolar-capillary barrier. Inflammatory mediators are...
Structural similarity between the flagellar type III ATPase FliI and F1-ATPase subunits
Imada, Katsumi, Minamino, Tohru, Tahara, Aiko, Namba, Keiichi
Construction of the bacterial flagellum in the cell exterior proceeds at its distal end by highly ordered self-assembly of many different component proteins, which are selectively exported through...
Saijo-Hamano, Yumiko, Imada, Katsumi, Minamino, Tohru, Kihara, May, Macnab, Robert M., Namba, Keiichi
Crystals of the cytoplasmic domain of FlhA, a membrane-protein component of the bacterial flagellar type III protein-export apparatus from Salmonella, have been obtained and characterized by X-ray...
Matsuura, Katsuhisa, Wada, Hiroshi, Nagai, Toshio, Iijima, Yoshihiro, Minamino, Tohru, Sano, Masanori, ...
Cardiomyocytes fuse with surrounding noncardiomyocytes and reenter the cell cycle
Matsuura, Katsuhisa, Wada, Hiroshi, Nagai, Toshio, Iijima, Yoshihiro, Minamino, Tohru, Sano, Masanori, ...
The concept of the plasticity or transdifferentiation of adult stem cells has been challenged by the phenomenon of cell fusion. In this work, we examined whether neonatal cardiomyocytes fuse with...
Kojima, Seiji, Furukawa, Yukio, Matsunami, Hideyuki, Minamino, Tohru, Namba, Keiichi
MotA and MotB are integral membrane proteins that form the stator complex of the proton-driven bacterial flagellar motor. The stator complex functions as a proton channel and couples proton flow with...
Minamino, Tohru, Imada, Katsumi, Tahara, Aiko, Kihara, May, Macnab, Robert M., Namba, Keiichi
Crystals of an N-terminally truncated variant of the Salmonella flagellar ATPase FliI, which exports substrate proteins into the central channel of the growing flagellar structure by utilizing the...
IGFBP-4 is an inhibitor of canonical Wnt signalling required for cardiogenesis
Zhu, Weidong, Shiojima, Ichiro, 塩島, 一朗, Ito, Yuzuru, Li, Zhi, Ikeda, Hiroyuki, ...
Insulin-like growth factor binding proteins (IGFBPs) bind to and modulate the actionsof insulin-like growth factors (IGFs). Although some of the actions of IGFBPs havebeen reported to be independent...
Che, Yong-Suk, Nakamura, Shuichi, Kojima, Seiji, Kami-ike, Nobunori, Namba, Keiichi, Minamino, Tohru
MotA and MotB form the stator of the proton-driven bacterial flagellar motor, which conducts protons and couples proton flow with motor rotation. Asp-33 of Salmonella enterica serovar Typhimurium...