Tom Brown

Flexible acoustic particle manipulation device with integrated optical waveguide for enhanced microbead assays (2009)

Glynne-Jones, Peter, Boltryk, Rosemary J., Hill, Martyn, Zhang, Fan, Dong, Liqin, Wilkinson, James S., ...

Realisation of a device intended for the manipulation and detection of bead-tagged DNA and other bio-molecules is presented. Acoustic radiation forces are used to manipulate polystyrene micro-beads...

DNA triplex formation with 5-dimethylaminopropargyl deoxyuridine (2009)

Rusling, David A., Peng, Guomei, Srinivasan, Natarajan, Fox, Keith R., Brown, Tom

We have prepared triplex-forming oligonucleotides containing the nucleotide analogue 5-dimethylaminopropargyl deoxyuridine (DMAPdU) in place of thymidine and examined their ability to form...

Stable DNA triple helix formation (2009)

Fox, Keith R., Cardew, Antonia S., Vergara, Nuria, Brown, Tom

Triplex-forming oligonucleotides bind in the major groove of duplex DNA, generating three stranded structures containing T.AT and C+.GC triplets. Their sequence specific binding has potential uses in...

The information contained in this document is subject to change without notice. CONTRIBUTING WRITERS (2008)

Andrew Macdonald, All Rights Reserved, Tom Brown, Julio Andrade, Erik Hoel, Jonathan Bailey

U.S. GOVERNMENT RESTRICTED/LIMITED RIGHTS Any software, documentation, and/or data delivered hereunder is subject to the terms of the License Agreement. In no event shall the U.S. Government acquire...

Rapid and efficient DNA strand cross-linking by click chemistry (2008)

Kocalka, Petr, El-Sagheer, Afaf H., Brown, Tom

Click chemistry has been used to covalently cross-link complementary DNA strands between bases to form very stable duplexes. Several alkyne- and azide-modified uracil monomers were used to evaluate...

Characterization and use of an unprecedentedly bright and structurally non-perturbing fluorescent DNA base analogue (2008)

Sandin, Peter, Borjesson, Karl, Li, Hong, Martensson, Jerker, Brown, Tom, Wilhelmsson, L. Marcus, ...

This article presents the first evidence that the DNA base analogue 1,3-diaza-2-oxophenoxazine, tCO, is highly fluorescent, both as free nucleoside and incorporated in an arbitrary DNA structure. tCO...

Controlled release of Chol-TEG-DNA from nano- and micropatterned SU-8 surfaces by a spreading lipid film (2008)

Erkan, Yavuz, Halma, Kiona, Czolkos, Ilja, Jesorka, Aldo, Dommersnes, Paul, Kumar, Ravindra, ...

We report the controlled release of immobilized cholesteryl-tetraethyleneglycol-DNA (chol-DNA) from micropatterned SU-8 surfaces by a spreading lipid film. The release of chol-DNA is rapid and on the...

Kinetic studies on the formation of DNA triplexes containing the nucleoside analogue 2 '-O-(2-aminoethyl)-5-(3-amino-1-propynyl)uridine (2008)

Rusling, David A., Broughton-Head, Victoria J., Tuck, Alex, Khairallah, Hannah, Osborne, Sadie D., Brown, Tom, ...

We have examined the kinetics of triple helix formation of oligonucleotides that contain the nucleotide analogue 2-O-(2-aminoethyl)-5-(3-amino-1-propynyl)uridine (bis-amino-U, BAU), which forms very...

Characterization and use of an unprecedentedly bright and structurally non-perturbing fluorescent DNA base analogue (2008)

Sandin, Peter, Börjesson, Karl, Li, Hong, Mårtensson, Jerker, Brown, Tom, Wilhelmsson, L. Marcus, ...

This article presents the first evidence that the DNA base analogue 1,3-diaza-2-oxophenoxazine, tCO, is highly fluorescent, both as free nucleoside and incorporated in an arbitrary DNA structure. tCO...

Characterization and Use of Tricyclic Fluorescent Nucleic Acid Base Analogues (2008)

Börjesson, Karl, Sandin, Peter, Albinsson, Bo, Mårtensson, Jerker, Brown, Tom, Wilhelmsson, L. Marcus

The two recently developed nucleic acid probe molecules tC and tCO both have unique properties compared to other molecules in the family of fluorescent base analogues.1–5 These tricyclic base...

Addressable Molecular Node Assembly - High Information Density DNA Nanostructures (2008)

Lundberg, Erik, Tumpane, John, Kumar, Ravindra, Sandin, Peter, Gale, Nittaya, Nandhakumar, Iris S., ...

The inherent self-assembly properties of DNA make it ideal in nanotechnology. We present a fully addressable DNA nanostructure with the smallest possible unit cell, a hexagon with a side-length of...

A Membrane Anchored DNA-based Energy/Electron Transfer Assembly (2008)

Börjesson, Karl, Tumpane, John, Ljungdahl, Thomas, Wilhelmsson, L. Marcus, Nordén, Bengt, Brown, Tom, ...

In this work the trapping and conversion of visible light energy into chemical energy is examined using a supramolecular assembly. This consists of a light absorbing antenna and a porphyrin redox...

Addressable Molecular Node Assembly - Functional DNA Nanostructures (2008)

Tumpane, John, Lundberg, Erik P., Wilhelmsson, L. Marcus, Brown, Tom, Nordén, Bengt

The use of nucleic acids as a nanomaterial is becoming increasingly widespread due to the suitability of the hydrogen-bonding patterns and sequence specificity inherent to the double-helix. As...

Spectacle/gender/history: the case of Gone with the Wind (2008)

Brown, Tom

The essay asserts that, since pioneering work in the 1970s and 80s (in Screen in particular), the study of classical Hollywood cinema has failed adequately to acknowledge and understand the role of...

Triplex addressability as a basis for functional DNA nanostructures (2007)

Tumpane, John, Kumar, Ravindra, Lundberg, Erik P., Sandin, Peter, Gale, Nittaya, Nandhakumar, Iris S., ...

Here, we present the formation of a fully addressable DNA nanostructure that shows the potential to be exploited as, for example, an information storage device based on pH-driven triplex strand...

Evolution Of Topography During 1 (2007)

Step Cmp Of, Steve Hymes, Tom Brown, Paul Lefevre, Bob Mikkola, Ishmail Emesh

Electroplate has emerged as a viable deposition technique for advanced copper-based metallization. Wafer-scale, array-scale and feature-scale metal thickness nonuniformity occurs during the plating...

DIRECT MEASUREMENT OF PLANARIZATION LENGTH FOR COPPER CHEMICAL MECHANICAL PLANARIZATION POLISHING (CMP) PROCESSES USING A LARGE PATTERN TEST MASK (2007)

Paul Lefevre, Albert Gonzales, Tom Brown, Gerald Martin, International Sematech, Ma Michael Gostein, ...

We have used a large pattern test mask and a specific arrangement of structures on a wafer for direct measurement of an average planarization length for copper chemical mechanical polishing (CMP)...

DIRECT MEASUREMENT OF PLANARIZATION LENGTH FOR COPPER CHEMICAL MECHANICAL PLANARIZATION POLISHING (CMP) PROCESSES USING A LARGE PATTERN TEST MASK (2007)

Paul Lefevre, Albert Gonzales, Tom Brown, Gerald Martin, International Sematech, Ma Michael Gostein, ...

We have used a large pattern test mask and a specific arrangement of structures on a wafer for direct measurement of an average planarization length for copper chemical mechanical polishing (CMP)...

2 '-O-dimethylaminoethoxyuridine and 5-dimethylaminopropargyl deoxyuridine for at base pair recognition in triple helices (2007)

Brennan, Lavinia, Peng, Guomei, Srinivasan, Natarajan, Fox, Keith R., Brown, Tom

The nucleoside analogues 2'-O-dimethylaminoethoxy uridine and 5-dimethylaminopropargyl deoxyuridine have been synthesised and incorporated into oligonucleotides. Their triplex-stabilising properties...

Cg base pair recognition within DNA triple helices using N-methyl-3H-pyrrolo[2,3-d]pyrimidin-2(7H)-one nucleoside analogues (2007)

Gerrard, Simon R., Srinivasan, Natarajan, Fox, Keith R., Brown, Tom

Triplex-mediated recognition of Py.Pu base pairs in DNA is a greater challenge than for Pu.Py base pairs as fewer hydrogen bonds are presented for binding in the major groove. Initial studies on...

Photoinduced crosslinking of double-helical DNA by psoralen covalently linked to a triple helix-forming oligonucleotide under near-physiological conditions (2007)

Li, Hong, Broughton-Head, Victoria J., Fox, Keith R., Brown, Tom

Stable triplexes have been generated under near-physiological conditions by the introduction of the C and T base analogues 3-methyl-2-aminopyridine-2 '-deoxyriboside and...

Synthesis of anthraquinone oligonucleotides for triplex stabilization (2007)

Zhao, Zhengyun, Peng, Guomei, Michels, Jan, Fox, Keith R., Brown, Tom

The synthesis of two anthraquinone phosphoramidites is described. In both cases the anthraquinone moiety is attached via a linker to the 5-position of a uracil base, allowing incorporation at any...

Intramolecular DNA quadruplexes with different arrangements of short and long loops (2007)

Rachwal, Phillip A., Findlow, I. Stuart, Werner, Joern M., Brown, Tom, Fox, Keith R.

We have examined the folding, stability and kinetics of intramolecular quadruplexes formed by DNA sequences containing four G3 tracts separated by either single T or T4 loops. All these sequences...

Synthesis and properties of oligonucleotides containing a cholesterol thymidine monomer (2007)

Durand, Adeline, Brown, Tom

Highly selective base-pair recognition makes DNA a suitable building block for orderly self-assembled structures. For some applications in nanotechnology DNA complexes need to be fixed onto surfaces....

Naphthalenyl- and anthracenyl-ethynyl dT analogues as base discriminating fluorescent nucleosides and intramolecular energy transfer donors in oligonucleotide probes (2007)

Xiao, Qiang, Ranasinghe, Rohan T., Tang, Adrian M.P., Brown, Tom

Fluorescent thymidine analogues functionalised in the 5-position with the moieties naphthalenylethynyl (NeT), anthracenylethynyl (AeT) and anthracenylbuta-1,3-diynyl (AeeT) have been incorporated...

Sequence effects of single base loops in intramolecular quadruplex DNA (2007)

Rachwal, Phillip A., Brown, Tom, Fox, Keith R.

We have examined the properties of intramolecular G-quadruplexes in which the G3 tracts are separated by single base loops. The most stable complex contained 1′,2′-dideoxyribose in all three...

Electrospray ionisation-cleavable tandem nucleic acid mass tag-peptide nucleic acid conjugates: synthesis and applications to quantitative genomic analysis using electrospray ionisation-MS/MS (2007)

Thompson, Andrew, Prescott, Mark, Chelebi, Noorhan, Smith, John, Brown, Tom, Schmidt, Günter

The synthesis and characterization of isotopomer tandem nucleic acid mass tag–peptide nucleic acid (TNT–PNA) conjugates is described along with their use as electrospray ionisation-cleavable...

Effect of G-tract length on the topology and stability of intramolecular DNA quadruplexes (2007)

Rachwal, Philip A., Brown, Tom, Fox, Keith R.

G-Rich sequences are known to form four-stranded structures that are based on stacks of G-quartets, and sequences with the potential to adopt these structures are common in eukaryotic genomes....

Factors that may contribute to the establishment of mobile learning in institutions – Results from a Survey (2007)

Olaf Zawacki-Richter, Tom Brown, Rhena Delport

This paper reports on a survey investigating the role that differences in expectations and perceptions of mobile learning and mobile devices play in establishing mobile learning at an educational...

Template-directed oligonucleotide strand ligation, covalent intramolecular DNA circularization and catenation using click chemistry (2007)

Kumar, Ravindra, El-Sagheer, Afaf, Tumpane, John, Lincoln, Per, Wilhelmsson, L. Marcus, Brown, Tom

The copper-catalyzed azide-alkyne cycloaddition reaction has been used for the template-mediated chemical ligation of two oligonucleotide strands, one with a 5'-alkyne and the other with a 3'-azide,...

The Lion King and the Digital Theme Park (2007)

Tom Brown, Tom Brown

Citations (this article cites 4 articles hosted on the

Electrospray ionisation-cleavable tandem nucleic acid mass tag-peptide nucleic acid conjugates: synthesis and applications to quantitative genomic analysis using electrospray ionisation-MS/MS (2007)

Thompson, Andrew, Prescott, Mark, Chelebi, Noorhan, Smith, John, Brown, Tom, Schmidt, Günter

The synthesis and characterization of isotopomer tandem nucleic acid mass tag–peptide nucleic acid (TNT–PNA) conjugates is described along with their use as electrospray ionisation-cleavable...

Triplex staples: DNA double-strand cross-linking at internal and terminal sites using psoralen-containing triplex-forming oligonucleotides (2006)

Li, Hong, Broughton-Head, Victoria J., Peng, Guomei, Powers, Vicki E.C., Ovens, Matthew J., Fox, Keith R.., ...

A method has been developed to attach 4'-(hydroxymethyl)-4,5',8-trimethylpsoralen to the 5 position of thymine bases during solid-phase oligonucleotide synthesis. UV irradiation of triplex-forming...

DNA triple-helix formation at target sites containing duplex mismatches (2006)

Rusling, David A., Brown, Tom, Fox, Keith R.

We have studied the formation of DNA triple helices at target sites that contain mismatches in the duplex target. Fluorescence melting studies were used to examine a series of parallel triple helices...

DNA sequence recognition by an imidazole-containing isopropyl-substituted thiazole polyamide (thiazotropsin B) (2006)

Hampshire, Andrew J., Khairallah, Hannah, Khalaf, Abedawn I., Ebrahimabadi, Abdolrasoul H., Waigh, Roger D., Suckling, Colin J., ...

We have used DNA footprinting and fluorescence melting experiments to study the sequence specific binding of an imidazole-containing isopropyl-substituted thiazole polyamide (thiazotropsin B) to DNA....

One-to-one technology-enhanced learning: an opportunity for global research collaboration (2006)

Chan, Tak-Wai, Roschelle, Jeremy, Hsi, Sherry, Kinshuk, Kinshuk, Sharples, Mike, Brown, Tom, ...

Over the next 10 years, we anticipate that personal, portable, wirelessly-networked technologies will become ubiquitous in the lives of learners - indeed, in many countries, this is already a...

One-to-one technology-enhanced learning: an opportunity for global research collaboration (2006)

Chan, Tak-Wai, Roschelle, Jeremy, Hsi, Sherry, Kinshuk, Kinshuk, Sharples, Mike, Brown, Tom, ...

Over the next 10 years, we anticipate that personal, portable, wirelessly-networked technologies will become ubiquitous in the lives of learners - indeed, in many countries, this is already a...

Combining nucleoside analogues to achieve recognition of oligopurine tracts by triplex-forming oligonucleotides at physiological pH (2005)

Rusling, David A., Le Strat, Loic, Powers, Vicki E.C., Broughton-Head, Victoria J., Booth, James, Lack, Oliver, ...

We have used DNase I footprinting to examine DNA triple helix formation at a 12 base pair oligopurine · oligopyrimidine sequence, using oligonucleotides that contain combinations of...

An extra dimension in nucleic acid sequence recognition (2005)

Fox, Keith R., Brown, Tom

Introduction 312 Triple helices in DNA 312 Chemically modified TFOs 313 Further development 316 Recognition of GC base pairs 316 Recognition of TA base pairs 316 Recognition of AT base pairs 317...

Quenching of CdSe quantum dot emission, a new approach for biosensing (2005)

Melvin, Tracy, Dyadyusha, Larysa, Yin, Huabing, Jaiswal, Supriya, Baumberg, Jeremy, Booy, Frank, ...

The emission of CdSe quantum dots linked to the 5-end of a DNA sequence is efficiently quenched by hybridisation with a complementary DNA strand with a gold nanoparticle attached at the 3-end;...

Quenching of CdSe quantum dot emission, a new approach for biosensing (2005)

Melvin, Tracy, Dyadyusha, Larysa, Yin, Huabing, Jaiswal, Supriya, Baumberg, Jeremy, Booy, Frank, ...

The emission of CdSe quantum dots linked to the 5-end of a DNA sequence is efficiently quenched by hybridisation with a complementary DNA strand with a gold nanoparticle attached at the 3-end;...

Stable recognition of TA interruptions by triplex forming oligonucleotides containing a novel nucleoside (2005)

Wang, Yang, Rusling, David A., Powers, Vicki E.C., Lack, Oliver, Osborne, Sadie D., Fox, Keith R., ...

We have prepared the 2'-aminoethoxy derivative of the S nucleoside (2AES) and incorporated it into triplex-forming oligonucleotides for recognition of TA interruptions within a target oligopurine...

Four base recognition by triplex-forming oligonucleotides at physiological pH (2005)

Rusling, David.A., Powers, Vicki .E.C., Ranasinghe, Rohan .T., Wang, Yang, Osborne, Sadie .D., Brown, Tom., ...

The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for...

Task Assignment for a Physical Agent Team via a Dynamic (2005)

Forward Reverse Auction, Amr Ahmed, Abhilash Patel, Tom Brown, Myungjoo Ham, Myeong-wuk Jang, ...

In the dynamic distributed task assignment (DDTA) problem, a team of agents is required to accomplish a set of tasks while maximizing the overall team utility. An effective solution to this problem...

TABLE OF CONTENTS (2005)

Martin Vandepas, Ting Wu, Tom Brown, Vova Kratyuk, Gil-cho Ahn, Neal Chapman

My mother and father have offered me a constant, unwavering umbrella of support throughout my life. They have encouraged me to think for myself and chase my dreams. The belief that any goal is...

Task assignment for a physical agent team via a dynamic forward/reverse auction mechanism (2005)

Amr Ahmed, Abhilash Patel, Tom Brown, Myungjoo Ham, Myeong-wuk Jang, Gul Agha

Abstract ⎯ In the dynamic distributed task assignment (DDTA) problem, a team of agents is required to accomplish a set of tasks while maximizing the overall team utility. An effective solution to...

Fluorescent properties of DNA base analogue tC upon incorporation into DNA -- negligible influence of neighbouring bases on fluorescence quantum yield (2005)

Sandin, Peter, Wilhelmsson, L. Marcus, Lincoln, Per, Powers, Vicki E. C., Brown, Tom, Albinsson, Bo

The quantum yield of the fluorescent tricyclic cytosine analogue, 1,3-diaza-2-oxophenothiazine, tC, is high and virtually unaffected by incorporation into both single- and double-stranded DNA...

Four base recognition by triplex-forming oligonucleotides at physiological pH (2005)

Rusling, David A., Powers, Vicki E. C., Ranasinghe, Rohan T., Wang, Yang, Osborne, Sadie D., Brown, Tom, ...

We have achieved recognition of all 4 bp by triple helix formation at physiological pH, using triplex-forming oligonucleotides that contain four different synthetic nucleotides. BAU...

DNA sequence recognition by an isopropyl substituted thiazole polyamide (2004)

James, Peter L., Merkina, Elena E., Khalaf, Abedawn I., Suckling, Colin J., Waigh, Roger D., Brown, Tom, ...

We have used DNA footprinting and fluorescence melting experiments to study the sequence-specific binding of a novel minor groove binding ligand (thiazotropsin A), containing an isopropyl substituted...

Selectivity and affinity of triplex-forming oligonucleotides containing 2'-aminoethoxy-5-(3-aminoprop-1-ynyl)uridine for recognizing AT base pairs in duplex DNA (2004)

Osborne, Sadie D., Powers, Vicki E. C., Rusling, David A., Lack, Oliver, Fox, Keith R., Brown, Tom

We have used DNase I footprinting, fluorescence and ultraviolet (UV) melting experiments and circular dichroism to demonstrate that, in the parallel triplex binding motif,...

DNA adopts normal B-form upon incorporation of highly fluorescent DNA base analogue tC: NMR structure and UV-Vis spectroscopy characterization (2004)

Engman, K. Cecilia, Sandin, Peter, Osborne, Sadie, Brown, Tom, Billeter, Martin, Lincoln, Per, ...

The influence of the highly fluorescent tricyclic cytosine base analogue (tC) on duplex DNA conformation is investigated. The duplex properties are characterized by absorbance and circular dichroism...

DNA sequence recognition by an isopropyl substituted thiazole polyamide (2004)

James, Peter L., Merkina, Elena E., Khalaf, Abedawn I., Suckling, Colin J., Waigh, Roger D., Brown, Tom, ...

We have used DNA footprinting and fluorescence melting experiments to study the sequence-specific binding of a novel minor groove binding ligand (thiazotropsin A), containing an isopropyl substituted...

The solution structure of a DNA{middle dot}RNA duplex containing 5-propynyl U and C; comparison with 5-Me modifications (2003)

Gyi, Jeffrey I., Gao, Daquan, Conn, Graeme L., Trent, John O., Brown, Tom, Lane, Andrew N.

The addition of the propynyl group at the 5 position of pyrimidine nucleotides is highly stabilising. We have determined the thermodynamic stability of the DNA·RNA hybrid...

Thermodynamic and kinetic stability of intermolecular triple helices containing different proportions of C+{middle dot}GC and T{middle dot}AT triplets (2003)

James, Peter L., Brown, Tom, Fox, Keith R.

We have used oligonucleotides containing appropriately placed fluorophores and quenchers to measure the stability of 15mer intermolecular triplexes with third strands consisting of repeats of TTT,...

Crystal structure of the Escherichia coli dcm very-short-patch DNA repair endonuclease bound to its reaction product-site in a DNA superhelix (2003)

Bunting, Karen A., Roe, S. Mark, Headley, Anthony, Brown, Tom, Savva, Renos, Pearl, Laurence H.

Very‐short‐patch repair (Vsr) enzymes occur in a variety of bacteria, where they initiate nucleotide excision repair of G:T mismatches arising by deamination of 5‐methyl‐cytosines...

High throughput measurement of duplex, triplex and quadruplex melting curves using molecular beacons and a LightCycler (2002)

Darby, Richard A.J., Sollogoub, Matthieu, McKeen, Catherine, Brown, Lynda, Risitano, Antonina, Brown, Nicholas, ...

We have used oligonucleotides containing molecular beacons to determine melting profiles for intramolecular DNA duplexes, triplexes and quadruplexes (tetraplexes). The synthetic oligonucleotides used...

High throughput measurement of duplex, triplex and quadruplex melting curves using molecular beacons and a LightCycler (2002)

Darby, Richard A. J., Sollogoub, Matthieu, McKeen, Catherine, Brown, Lynda, Risitano, Antonina, Brown, Nicholas, ...

We have used oligonucleotides containing molecular beacons to determine melting profiles for intramolecular DNA duplexes, triplexes and quadruplexes (tetraplexes). The synthetic oligonucleotides used...

Duplex Scorpion primers in SNP analysis and FRET applications (2001)

Solinas, Antonio, Brown, Lynda J., McKeen, Catherine, Mellor, John M., Nicol, Jamie, Thelwell, Nicky, ...

Scorpions are fluorogenic PCR primers with a probe element attached at the 5′-end via a PCR stopper. They are used in real-time amplicon-specific detection of PCR products in homogeneous solution....

Recognition of GT mismatches by Vsr mismatch endonuclease (2000)

Fox, Keith R., Allinson, Sarah L., Sahagun-Krause, Heidi, Brown, Tom

The Vsr mismatch endonuclease recognises the sequence CTWGG (W = A or T) in which the underlined thymine is paired with guanine and nicks the DNA backbone on the 5′-side of the mispaired...

Mode of action and application of Scorpion primers to mutation detection (2000)

Thelwell, Nicola, Millington, Stephen, Solinas, Antonio, Booth, James, Brown, Tom

Scorpion primers can be used to detect PCR products in homogeneous solution. Their structure promotes a unimolecular probing mechanism. We compare their performance with that of the same probe...

Multi-Level Pattern Effects In Copper Cmp (1999)

Tae Park Tamba, Tae Park, Tamba Tugbawa, Duane Boning, Steve Hymes, Tom Brown, ...

In this paper, multi-level pattern dependent variations are described and characterized with newly designed test structures. The test vehicle used is a set of two level (metal 1 and metal 2) masks...

A Mathematical Model of Pattern Dependencies in Cu CMP Processes (1999)

Tamba Tugbawa, Tae Park, Duane Boning, Tony Pan, Ping Li, Steve Hymes, ...

In our previous work, we discussed the framework of a mathematical model of pattern dependencies in Cu CMP processes (1). In this paper, we present the detailed formulation of the model, propose a...

Retaining minority students at UW-Green Bay / (1993)

Brown, Tom.

Includes bibliographical references (leaves 61-63).

Synthesis and antibody-mediated detection of oligonucleotides containing multiple 2,4-dinitrophenyl reporter groups (1993)

Grzybowski, John, Will, David W., Randall, R.E., Smith, Clive A., Brown, Tom

A series of non-nucleoslde-based 2,4-dlnltrophenyl (DNP) phosphoramidltes have been prepared and used in the multiple labelling of ollgonucleotldes during solid-phase synthesis. The length of spacer...

Thermodynamic stability and drug-binding properties of oligodeoxyribonucleotide duplexes containing 3-deazaadenine:thymine base pairs (1993)

Lever, Catherine, Li, Xiang, Cosstick, Richard, Edel, Susanne, Brown, Tom

We have used ultraviolet melting techniques to study the effect on stability of incorporating the nucleoslde analogue 2'-deoxy-3-deazaadenoslne (d3CA) into the duplex 5'-d(CGCAATCG) 3'-d(GCGTTAGC)....

The molecular structure of a 4'-epiadrimamycin complex with d(TGATCA) at 1.7A resolution: comparison with the structure of 4'-epiadriamycin d(TGTACA) and d(CGATCG) complexes (1992)

D'Estaintot, Beatrice Langlois, Gallois, Bernard, Brown, Tom, Hunter, William N.

The structure of the complex between d(TGATCA) and the anthracycllne 4′-epladriamycln has been determined by crystallographlc methods. The crystals are tetragonal, space group P41212 with unit cell...

The conformational variability of an adenosine inosine base-pair in a synthetic DNA dodecamer (1992)

Leonard, Gordon A., Booth, Ewan D., Hunter, William N., Brown, Tom

A crystal structure analysis of the synthetic deoxydodecamer d(CGCAAATTIGCG) which contains two adenosine·Inosine (A·I) mispairs has revealed that, in this sequence, the A·I base-pairs adopt a...

Synthesis and physical properties of anti-HIV antisense oligonucleotides bearing terminal lipophilic groups (1992)

MacKellar, Calum, Graham, Duncan, Will, David W., Burgess, Stephen, Brown, Tom

A number of phosphoramldite monomers have been prepared and used in the synthesis of antisense phosphorothioate oligonucleotides bearing 5′-polyalkyl and cholesterol moieties. Similar groups have...

Structural and thermodynamic studies on the adenine.guanine mismatch in B-DNA (1990)

Leonard, Gordon A., Booth, Ewan D., Brown, Tom

The structure of the synthetic dodecamer d(CGCAAATTGGCG) has been shown by single crystal X-ray diffraction methods to be that of a B-DNA helix containing two A(anti).G(syn) base pairs. The...

Refined crystal structure of an octanucleotide duplex with I.T. mismatched base pairs (1989)

Cruse, W.B.T., Aymani, J., Kennard, Olga, Brown, Tom, Jack, Audrey G.C., Leonard, G.A.

The structure of the synthetic deoxyoctamer d(GGIGCTCC) has been determined by single crystal X-ray diffraction techniques to a resolution of 1.7Å. The sequence crystallises in space group P61, with...

Structural features and hydration of a dodecamer duplex containing two C.A mispairs (1987)

Hunter, William N., Brown, Tom, Kennard, Olga

X-ray diffraction techniques have been used to characterise the crystal and molecular structure of the deoxyoligomer d(C-G-C-A-A-A-T-T-C-G-C-G) at 2.5Å resolution. The final R factor is 0.19 with...

Inosine.adenine base pairs in a B-DNA duplex (1987)

Corfield, Peter W.R., Hunter, William N., Brown, Tom, Robinson, Paul, Kennard, Olga

The structure of the synthetic deoxydodecamer d(C-G-C-I-A-A-T-T-A-G-C-G) has been determined by single crystal x-ray diffraction techniques at 2.5A resolution. The refinement converged with a...

Characterization and use of an unprecedentedly bright and structurally non-perturbing fluorescent DNA base analogue

Sandin, Peter, Börjesson, Karl, Li, Hong, Mårtensson, Jerker, Brown, Tom, Wilhelmsson, L. Marcus, ...

This article presents the first evidence that the DNA base analogue 1,3-diaza-2-oxophenoxazine, tCO, is highly fluorescent, both as free nucleoside and incorporated in an arbitrary DNA structure. tCO...

The construction of the credible: Epistolary transformations and the origins of the business letter

Ingrid Jeacle, Tom Brown

The letter is the formal mechanism for communication in the business community. Even contemporary advances in electronic mail have not yet displaced the legitimacy invested in this form of...

PHYSICAL PRODUCTIVITY

Brown, Tom

Productivity Analysis,

DNA triplex formation with 5-dimethylaminopropargyl deoxyuridine

Rusling, David A., Peng, Guomei, Srinivasan, Natarajan, Fox, Keith R., Brown, Tom

We have prepared triplex-forming oligonucleotides containing the nucleotide analogue 5-dimethylaminopropargyl deoxyuridine (DMAPdU) in place of thymidine and examined their ability to form...

Current measurement in applied behavior analysis

Springer, Bonnie, Brown, Tom, Duncan, Philip K.

The analysis of behavior began with a form of data, rate of responding, which allowed for efficient study and for the description of the basic principles of behavior. Especially important were the...