Ramesh Hariharan, Kartik Ch, Max L. Nibert, Tomas Kirchhausen, Tomas Kirchhausen
of clathrin-coated pits
Schaefer, Andrew W., Kamei, Yoshimasa, Kamiguchi, Hiroyuki, Wong, Eric V., Rapoport, Iris, Kirchhausen, Tomas, ...
Dynamic regulation of the cell surface expression of adhesion molecules is an important mechanism for controlling neuronal growth cone motility and guidance. Clathrin-mediated vesicular...
Massoud Ramezani Rad, Huan L. Phan, L. Kirchrath, Philip K. Tan, Tomas Kirchhausen, Cornelius P. Hollenberg, ...
Clathrin-coated vesicles mediate selective intracellular protein traffic from the plasma membrane and the trans-Golgi network. At these sites, clathrin-associated protein (AP) complexes have been...
Peptide-in-groove interactions link target proteins to the β-propeller of clathrin
Ter Haar, Ernst, Harrison, Stephen C., Kirchhausen, Tomas
The “WD40” domain is a widespread recognition module for linking partner proteins in intracellular networks of signaling and sorting. The clathrin amino-terminal domain, which directs...
Sanderfoot, Anton A., Ahmed, Sharif U., Marty-Mazars, Danièle, Rapoport, Iris, Kirchhausen, Tomas, Marty, Francis, ...
Targeting of protein cargo to the vacuole/lysosome is a multistep process that appears to have conserved features between mammalian, yeast, and plant cells. In each case, some soluble...
Structure of the Sec23p/24p and Sec13p/31p complexes of COPII
Lederkremer, Gerardo Z., Cheng, Yifan, Petre, Benjamin M., Vogan, Erik, Springer, Sebastian, Schekman, Randy, ...
COPII-coated vesicles carry proteins from the endoplasmic reticulum to the Golgi complex. This vesicular transport can be reconstituted by using three cytosolic components containing five proteins:...
Interaction of the Bovine Papillomavirus E6 Protein with the Clathrin Adaptor Complex AP-1
Tong, Xiao, Boll, Werner, Kirchhausen, Tomas, Howley, Peter M.
The E6 gene of the bovine papillomavirus type 1 (BPV-1) is expressed in fibropapillomas caused by BPV-1 and in tissue culture cells transformed by BPV-1. It encodes one of the two major oncoproteins...
Nieland, Thomas J. F., Penman, Marsha, Dori, Limor, Krieger, Monty, Kirchhausen, Tomas
The high-density lipoprotein (HDL) receptor, scavenger receptor, class B, type I (SR-BI), mediates both the selective uptake of lipids, mainly cholesterol esters, from HDL to cells and the efflux of...
Exo1: A new chemical inhibitor of the exocytic pathway
Feng, Yan, Yu, Sidney, Lasell, Troy K. R., Jadhav, Ashutosh P., Macia, Eric, Chardin, Pierre, ...
A phenotypic screen was used to search for drug-like molecules that can interfere with specific steps in membrane traffic. 2-(4-Fluorobenzoylamino)-benzoic acid methyl ester (Exo1), identified in...
Chandran, Kartik, Parker, John S. L., Ehrlich, Marcelo, Kirchhausen, Tomas, Nibert, Max L.
Cell entry by reoviruses requires a large, transcriptionally active subvirion particle to gain access to the cytoplasm. The features of this particle have been the subject of debate, but three...
Larsen, Jakob E., Massol, Ramiro H., Nieland, Thomas J. F., Kirchhausen, Tomas
HIV Nef has a number of important biological effects, including the down-modulation of several immunological important molecules (CD4, major histocompatibility complex [MHC] class I). Down-modulation...
Massol, Ramiro H., Larsen, Jakob E., Fujinaga, Yukako, Lencer, Wayne I., Kirchhausen, Tomas
Cholera toxin (CT) and related AB5 toxins bind to glycolipids at the plasma membrane and are then transported in a retrograde manner, first to the Golgi and then to the endoplasmic reticulum (ER). In...
Crystal structure of the clathrin adaptor protein 1 core
Heldwein, Ekaterina E., Macia, Eric, Wang, Jing, Yin, Helen L., Kirchhausen, Tomas, Harrison, Stephen C.
The heterotetrameric adaptor proteins (AP complexes) link the outer lattice of clathrin-coated vesicles with membrane-anchored cargo molecules. We report the crystal structure of the core of the AP-1...
Kamiguchi, Hiroyuki, Long, Kristin E., Pendergast, Maryanne, Schaefer, Andrew W., Rapoport, Iris, Kirchhausen, Tomas, ...
Cell–cell interactions mediated via cell adhesion molecules (CAMs) are dynamically regulated during nervous system development. One mechanism to control the amount of cell surface CAMs is to...
Feng, Yan, Jadhav, Ashutosh P, Rodighiero, Chiara, Fujinaga, Yukako, Kirchhausen, Tomas, Lencer, Wayne I
Cholera toxin (CT) follows a glycolipid-dependent entry pathway from the plasma membrane through the trans-Golgi network (TGN) to the endoplasmic reticulum (ER) where it is retro-translocated into...
The small chemical vacuolin-1 inhibits Ca2+-dependent lysosomal exocytosis but not cell resealing
Cerny, Jan, Feng, Yan, Yu, Anan, Miyake, Katsuya, Borgonovo, Barbara, Klumperman, Judith, ...
Resealing after wounding, the process of repair following plasma membrane damage, requires exocytosis. Vacuolins are molecules that induce rapid formation of large, swollen structures derived from...
Endocytosis is not required for the selective lipid uptake mediated by murine SR-BI
Nieland, Thomas J.F., Ehrlich, Marcelo, Krieger, Monty, Kirchhausen, Tomas
The scavenger receptor class B, type I (SR-BI) mediates the cellular selective uptake of cholesteryl esters and other lipids from high-density lipoproteins (HDL) and low-density lipoproteins (LDL)....
Peptide-in-groove interactions link target proteins to the β-propeller of clathrin
Ter Haar, Ernst, Harrison, Stephen C., Kirchhausen, Tomas
The “WD40” domain is a widespread recognition module for linking partner proteins in intracellular networks of signaling and sorting. The clathrin amino-terminal domain, which directs...
Sanderfoot, Anton A., Ahmed, Sharif U., Marty-Mazars, Danièle, Rapoport, Iris, Kirchhausen, Tomas, Marty, Francis, ...
Targeting of protein cargo to the vacuole/lysosome is a multistep process that appears to have conserved features between mammalian, yeast, and plant cells. In each case, some soluble...
Structure of the Sec23p/24p and Sec13p/31p complexes of COPII
Lederkremer, Gerardo Z., Cheng, Yifan, Petre, Benjamin M., Vogan, Erik, Springer, Sebastian, Schekman, Randy, ...
COPII-coated vesicles carry proteins from the endoplasmic reticulum to the Golgi complex. This vesicular transport can be reconstituted by using three cytosolic components containing five proteins:...
Interaction of the Bovine Papillomavirus E6 Protein with the Clathrin Adaptor Complex AP-1
Tong, Xiao, Boll, Werner, Kirchhausen, Tomas, Howley, Peter M.
The E6 gene of the bovine papillomavirus type 1 (BPV-1) is expressed in fibropapillomas caused by BPV-1 and in tissue culture cells transformed by BPV-1. It encodes one of the two major oncoproteins...
Nieland, Thomas J. F., Penman, Marsha, Dori, Limor, Krieger, Monty, Kirchhausen, Tomas
The high-density lipoprotein (HDL) receptor, scavenger receptor, class B, type I (SR-BI), mediates both the selective uptake of lipids, mainly cholesterol esters, from HDL to cells and the efflux of...
Exo1: A new chemical inhibitor of the exocytic pathway
Feng, Yan, Yu, Sidney, Lasell, Troy K. R., Jadhav, Ashutosh P., Macia, Eric, Chardin, Pierre, ...
A phenotypic screen was used to search for drug-like molecules that can interfere with specific steps in membrane traffic. 2-(4-Fluorobenzoylamino)-benzoic acid methyl ester (Exo1), identified in...
Chandran, Kartik, Parker, John S. L., Ehrlich, Marcelo, Kirchhausen, Tomas, Nibert, Max L.
Cell entry by reoviruses requires a large, transcriptionally active subvirion particle to gain access to the cytoplasm. The features of this particle have been the subject of debate, but three...
Larsen, Jakob E., Massol, Ramiro H., Nieland, Thomas J. F., Kirchhausen, Tomas
HIV Nef has a number of important biological effects, including the down-modulation of several immunological important molecules (CD4, major histocompatibility complex [MHC] class I). Down-modulation...
Massol, Ramiro H., Larsen, Jakob E., Fujinaga, Yukako, Lencer, Wayne I., Kirchhausen, Tomas
Cholera toxin (CT) and related AB5 toxins bind to glycolipids at the plasma membrane and are then transported in a retrograde manner, first to the Golgi and then to the endoplasmic reticulum (ER). In...
Crystal structure of the clathrin adaptor protein 1 core
Heldwein, Ekaterina E., Macia, Eric, Wang, Jing, Yin, Helen L., Kirchhausen, Tomas, Harrison, Stephen C.
The heterotetrameric adaptor proteins (AP complexes) link the outer lattice of clathrin-coated vesicles with membrane-anchored cargo molecules. We report the crystal structure of the core of the AP-1...
Feng, Yan, Jadhav, Ashutosh P, Rodighiero, Chiara, Fujinaga, Yukako, Kirchhausen, Tomas, Lencer, Wayne I
Cholera toxin (CT) follows a glycolipid-dependent entry pathway from the plasma membrane through the trans-Golgi network (TGN) to the endoplasmic reticulum (ER) where it is retro-translocated into...
The small chemical vacuolin-1 inhibits Ca2+-dependent lysosomal exocytosis but not cell resealing
Cerny, Jan, Feng, Yan, Yu, Anan, Miyake, Katsuya, Borgonovo, Barbara, Klumperman, Judith, ...
Resealing after wounding, the process of repair following plasma membrane damage, requires exocytosis. Vacuolins are molecules that induce rapid formation of large, swollen structures derived from...
A burst of auxilin recruitment determines the onset of clathrin-coated vesicle uncoating
Massol, Ramiro H., Boll, Werner, Griffin, April M., Kirchhausen, Tomas
Clathrin-coated pits assemble on a membrane and pinch off as coated vesicles. The released vesicles then rapidly lose their clathrin coats in a process mediated by the ATPase Hsc70, recruited by...
Wang, Jing, Sun, Hui-Qiao, Macia, Eric, Kirchhausen, Tomas, Watson, Hadiya, Bonifacino, Juan S., ...
Phosphatidylinositol 4 phosphate (PI4P) is highly enriched in the trans-Golgi network (TGN). Here we establish that PI4P is a key regulator of the recruitment of the GGA clathrin adaptor proteins to...
Endosomal recycling controls plasma membrane area during mitosis
Boucrot, Emmanuel, Kirchhausen, Tomas
The shape and total surface of a cell and its daughters change during mitosis. Many cells round up during prophase and metaphase and reacquire their extended and flattened shape during cytokinesis....
An ACAP1-containing clathrin coat complex for endocytic recycling
Li, Jian, Peters, Peter J., Bai, Ming, Dai, Jun, Bos, Erik, Kirchhausen, Tomas, ...
Whether coat proteins play a widespread role in endocytic recycling remains unclear. We find that ACAP1, a GTPase-activating protein (GAP) for ADP-ribosylation factor (ARF) 6, is part of a novel...
Mammalian Cells Change Volume during Mitosis
Boucrot, Emmanuel, Kirchhausen, Tomas
Using single cell-imaging methods we have found that the volume of adherent cells grown in culture decreases as the cells rounds when it enters mitosis. A minimal volume is reached at metaphase....
Schaefer, Andrew W., Kamei, Yoshimasa, Kamiguchi, Hiroyuki, Wong, Eric V., Rapoport, Iris, Kirchhausen, Tomas, ...
Dynamic regulation of the cell surface expression of adhesion molecules is an important mechanism for controlling neuronal growth cone motility and guidance. Clathrin-mediated vesicular...
Saffarian, Saveez, Kirchhausen, Tomas
We present a method to resolve components within a diffraction-limited object by tracking simultaneously the average axial positions of two different sets of fluorescent molecules within it. The...
Distinct Dynamics of Endocytic Clathrin-Coated Pits and Coated Plaques
Saffarian, Saveez, Cocucci, Emanuele, Kirchhausen, Tomas
Here we classify endocytic structures at the adherent (bottom) surface of many cells in culture into shorter-lived coated pits and longer-lived coated plaques which internalize by different...