Yamashita, Mitsuo, Kinoshita, Takuya, Ihara, Michiko, Mikawa, Tomomi, Murooka, Yoshikatu
To study the structure and function of pullulanase from Klebsiella aerogenes, a method involving random mutagenesis of the entire gene for pullulanase was used. Out of 50, 000 clones screened at high...