Yamagata, Shuzo, Yasugahira, Tomoko, Okuda, Yuriko, Iwama, Tomonori
l-Cystathionine &ggr;-lyase [EC 4.4.1.1] of Saccharomyces cerevisiae was shown to bind cofactor pyridoxal 5′-phosphate, up to 2 molecules/subunit. The association constants of the enzyme...
Gomi, Sumiko, Lee, Lan, Iwama, Tomonori, Imae, Yasuo
Thr-154 of the chemoreceptor Tar in Escherichia coli is important for aspartate sensing. Taking advantage of the fact that Tar has no Cys residues, we have further investigated the role of Thr-154 by...
Iwama, Tomonori, Nakao, Ko-Ichiro, Nakazato, Hiroshi, Yamagata, Shuzo, Homma, Michio, Kawagishi, Ikuro
The chemoreceptor Tcp mediates taxis to citrate. To identify citrate-binding residues, we substituted cysteine for seven basic or polar residues that are chosen based on the comparison of Tcp with...
Yamagata, Shuzo, Ichioka, Kazuhito, Goto, Koji, Mizuno, Yasuko, Iwama, Tomonori
A cell extract of an extremely thermophilic bacterium, Thermus thermophilus HB8, cultured in a synthetic medium catalyzed cystathionine γ-synthesis with O-acetyl-l-homoserine and l-cysteine as...
Yamagata, Shuzo, Akamatsu, Tsuyoshi, Iwama, Tomonori
Cystathionine γ-lyase of Saccharomyces cerevisiae was immobilized to aminohexyl-Sepharose through the cofactor pyridoxal 5′-phosphate and was characterized with respect to its cystathionine...
Iwama, Tomonori, Nakao, Ko-Ichiro, Nakazato, Hiroshi, Yamagata, Shuzo, Homma, Michio, Kawagishi, Ikuro
The chemoreceptor Tcp mediates taxis to citrate. To identify citrate-binding residues, we substituted cysteine for seven basic or polar residues that are chosen based on the comparison of Tcp with...
Yamagata, Shuzo, Ichioka, Kazuhito, Goto, Koji, Mizuno, Yasuko, Iwama, Tomonori
A cell extract of an extremely thermophilic bacterium, Thermus thermophilus HB8, cultured in a synthetic medium catalyzed cystathionine γ-synthesis with O-acetyl-l-homoserine and l-cysteine as...
Yamagata, Shuzo, Akamatsu, Tsuyoshi, Iwama, Tomonori
Cystathionine γ-lyase of Saccharomyces cerevisiae was immobilized to aminohexyl-Sepharose through the cofactor pyridoxal 5′-phosphate and was characterized with respect to its cystathionine...