Hosoya-Matsuda, Naomi, Inoue, Kazuhito, Hisabori, Toru
Thioredoxin is a small ubiquitous protein that is involved in the dithiol–disulfide exchange reaction, by way of two cysteine residues located on the molecule surface. In order to elucidate the...
Hishiya, Shoko, Hatakeyama, Wakako, Mizota, Yoko, Hosoya-Matsuda, Naomi, Motohashi, Ken, Ikeuchi, Masahiko, ...
Thioredoxin (Trx) is a small ubiquitous protein involved in the disulfide–dithiol exchange reaction occurring in cells and organelles. In vivo, Trx is reduced by Trx reductase using NADPH or...
Hisabori, Toru, Hara, Satoshi, Fujii, Tetsufumi, Yamazaki, Daisuke, Hosoya-Matsuda, Naomi, Motohashi, Ken
Thioredoxin affinity chromatography can be used to recognize the target proteins of thioredoxin or thioredoxin-related proteins in whole cells or certain cellular compartments. In the last couple of...
Hisabori, Toru, Hara, Satoshi, Fujii, Tetsufumi, Yamazaki, Daisuke, Hosoya-Matsuda, Naomi, Motohashi, Ken
Thioredoxin affinity chromatography can be used to recognize the target proteins of thioredoxin or thioredoxin-related proteins in whole cells or certain cellular compartments. In the last couple of...
Mitsuda, Nobutaka, Hisabori, Toru, Takeyasu, Kunio, Sato, Masa H.
A 38-bp pollen-specific cis-acting region of the AVP1 gene is involved in the expression of the Arabidopsis thaliana V-PPase during pollen development. Here, we report the isolation and structural...
Target Proteins of the Cytosolic Thioredoxins in Arabidopsis thaliana (2004)
Yamazaki, Daisuke, Motohashi, Ken, Kasama, Takeshi, Hara, Yukichi, Hisabori, Toru
Possible target proteins of cytosolic thioredoxin in higher plants have been investigated in the cell lysate of dark-grown Arabidopsis thaliana whole tissues. We immobilized a mutant of cytosolic...
Lill, Holger, Hisabori, Toru, Groth, Georg, Bald, Dirk
Structural and functional characterization of proteins is frequently hampered by lack of stability or by insufficient assembly of oligomeric proteins in over-expression systems. Using F1-ATPase as a...
Lill, Holger, Hisabori, Toru, Groth, Georg, Bald, Dirk
The structural and functional characterization of proteins is frequently hampered by lack of stability or by insufficient assembly of oligomeric proteins in over-expression systems. Using F1-ATPase...
Lill, Holger, Hisabori, Toru, Groth, Georg, Bald, Dirk
Structural and functional characterization of proteins is frequently hampered by lack of stability or by insufficient assembly of oligomeric proteins in over-expression systems. Using F1-ATPase as a...
Saze, Hidetoshi, Ueno, Yoshihisa, Hisabori, Toru, Hayashi, Hiroaki, Izui, Katsura
The activity of phosphoenolpyruvate carboxylase (PEPC, EC4.1.1.31) for the C4 photosynthesis is known to be regulated mainly in response to light/dark transitions through reversible...
Muneyuki, Eiro, Hisabori, Toru, Sasayama, Takeshi, Mochizuki, Katsura, Yoshida, Masasuke
The interactions of substoichiometric TNP-ATP and F1-ATPase from Escherichia coil (EF1) were examined and compared with those in the case of mitochondrial F1-ATPase (MF1) and F1-ATPase from...
Hisabori, Toru, Kobayashi, Hiromi, Kaibara, Chitose, Yoshida, Masasuke
F1 -ATPase isolated from plasma membrane of a thermophilic Bacillus strain PS3 (TF1) has very little or no endogenously bound adenine nucleotides. However, it can bind one ADP per mol of the enzyme...
Hisabori, Toru, Kothen, Gunther, Strotmann, Heinrich
Irradiation of isolated chloroplast thylakoids with TNP-ADP results in non-covalent binding and covalent incorporation of a reaction product of TNP-ADP formed by photo-synthetic reduction into the...
Ohta, Yoshinori, Yoshioka, Tatsuya, Mochimaru, Mari, Hisabori, Toru, Sakurai, Hidehiro
Tentoxin strongly inhibited the ATPase activity of isolated coupling factor 1 (AF1) from the cyanobacterium Anacystis nidulans, with 50% inhibition occurring at 0.3 μM. When thylakoids from A....
Hisabori, Toru, Mochizuki, Katsura
The inactivation and the activation of the ATPase of isolated CF1 as assayed by the hydrolysis of ATP in 10 s depended on prior binding of ADP-Mg and ATP-Mg. The effects of Mg2+ on the...
Nakazawa, Miki, Kakihana, Junko, Hisabori, Toru, Manabe, Katsushi
Procedures for the purification of native phytochrome from etiolated pea seedlings without the use of immuno-purification techniques are described. Phytochrome (in the PFR form) was purified by...
Reconstituted F1-ATPase Complexes Containing One Impaired {beta} Subunit Are ATPase-Active (1989)
Miwa, Keishi, Ohtsubo, Michihiro, Denda, Kimitoshi, Hisabori, Toru, Date, Takayasu, Yoshida, Masasuke
Homogeneous populations of hybrid α3β3γ complexes of the thermostable F1-ATPase containing one, two, or three copies of the mutationally impaired 0 subunits were produced using the solid phase...
ADP Binding to TF1 and Its Subunits Induces Ultraviolet Spectral Changes (1986)
HISABORI, Toru, YOSHIDA, Masasuke, SAKURAI, Hidehiro
Adenine nucleotide binding sites on the coupling factor ATPase of thermophilic bacterium PS3 (TF1) were investigated by UV spectroscopy and by equilibrium dialysis. When ADP was mixed with TF1 in the...
Hisabori, Toru, Sakurai, Hidehiro
The effects of methanol on the nucleotide binding to isolated chloroplast coupling factor 1 (CF1) were investigated. Isolated CF1 has four kinds of nucleotide binding sites; a barely dissociable...
Hisabori, Toru, Sakurai, Hidehiro
Properties of the nucleotide binding sites on chloroplast coupling factor 1 (CF1) were studied by equilibrium dialysis and UV spectroscopy. From our direct binding studies, we identified at least...
SAKURAI, Hidehiro, SHINOHARA, Kenji, HISABORI, Toru, SHINOHARA, Kunio
The ATPase activity of purified coupling factor 1 (CF1) of spinach chloroplasts [EC 3.6.1.3] was reversibly enhanced in some aqueous organic solvents, notably methanol, ethanol, and acetone....
Comprehensive survey of proteins targeted by chloroplast thioredoxin
Motohashi, Ken, Kondoh, Aiko, Stumpp, Michael T., Hisabori, Toru
Possible target proteins of chloroplast thioredoxin (Trx) have been investigated in the stroma lysate of spinach chloroplasts. For that purpose, we immobilized a mutant of m-type Trx in which an...
Comprehensive survey of proteins targeted by chloroplast thioredoxin
Motohashi, Ken, Kondoh, Aiko, Stumpp, Michael T., Hisabori, Toru
Possible target proteins of chloroplast thioredoxin (Trx) have been investigated in the stroma lysate of spinach chloroplasts. For that purpose, we immobilized a mutant of m-type Trx in which an...
The regulator of the F1 motor: inhibition of rotation of cyanobacterial F1-ATPase by the ɛ subunit
Konno, Hiroki, Murakami-Fuse, Tomoe, Fujii, Fumihiko, Koyama, Fumie, Ueoka-Nakanishi, Hanayo, Pack, Chan-Gi, ...
The chloroplast-type F1 ATPase is the key enzyme of energy conversion in chloroplasts, and is regulated by the endogenous inhibitor ɛ, tightly bound ADP, the membrane potential and the redox state...