Masahiro Yuki, Shigeharu Moriya, Tetsushi Inoue, Toshiaki Kudo
Microorganisms dwell symbiotically in the termite hindgut. In this study, we identified genes that contribute to the role of the host in maintaining this symbiotic relationship with microorganisms....
Molecular Phylogeny of Three Oxymonad Genera: Pyrsonympha, Dinenympha and Oxymonas (2003)
SHIGEHARU MORIYA, JOEL B. DACKS, AIKA TAKAGI, SATOKO NODA, MORIYA OHKUMA, W. FORD DOOLITTLE, ...
Oxymonads are a morphologically well-characterized and highly diverse lineage of protists. They are, however, under sampled at a molecular level. It has recently been demonstrated that a genus of...
Otagiri, Masato, Kurisu, Genji, Ui, Sadaharu, Takusagawa, Yusuke, Ohkuma, Moriya, Kudo, Toshiaki, ...
The crystal structure of a ternary complex of meso-2,3-butanediol dehydrogenase with NAD+ and a competitive inhibitor, mercaptoethanol, has been determined at 1.7 � resolution by means of molecular...
Oxymonads Are Closely Related to the Excavate Taxon Trimastix (2001)
Dacks, Joel B., Silberman, Jeffrey D., Simpson, Alastair G. B., Moriya, Shigeharu, Kudo, Toshiaki, Ohkuma, Moriya, ...
Despite intensive study in recent years, large-scale eukaryote phylogeny remains poorly resolved. This is particularly problematic among the groups considered to be potential early branches. In many...
Moriya Ohkuma, Kuniyo Ohtoko, Toshiya Iida, Mitsunori Tokura, Shigeharu Moriya, Ron Usami, ...
The phylogenetic diversity of parabasalian flagellates was examined based on the sequences of small subunit ribosomal RNA genes amplified directly from the mixed population of flagellates in the...
Cloning of Leucine Genes of Alkalophilic Bacillus No. 221 in E. coli and B. subtilis (1984)
HONDA, Hiroshi, KATO, Chiaki, KUDO, Toshiaki, HORIKOSHI, Koki
The leucine genes of alkalophilic Bacillus No. 221 were cloned into the HindIII sites of pBR322 and pGR71, and recombinant plasmids pHK101 and pHK111 were constructed. The cloned 7.2 kb DNA...
Ohkuma, Moriya, Noda, Satoko, Kudo, Toshiaki
Nitrogen fixation by the microorganisms in the gut of termites is one of the crucial aspects of symbiosis, since termites usually thrive on a nitrogen-poor diet. The phylogenetic diversity of the...
Noda, Satoko, Ohkuma, Moriya, Usami, Ron, Horikoshi, Koki, Kudo, Toshiaki
Expression of the nitrogen fixation gene, nifH, in the gut of the termite Neotermes koshunensis was characterized without cultivation. nifH cDNA was directly amplified from mRNA of the mixed...
Kosono, Saori, Ohashi, Yoshiaki, Kawamura, Fujio, Kitada, Makio, Kudo, Toshiaki
ShaA (sodium/hydrogen antiporter, previously termed YufT [or NtrA]), which is responsible for Na+/H+ antiporter activity, is considered to be the major Na+ excretion system in Bacillus subtilis. We...
Iida, Toshiya, Mukouzaka, Yuki, Nakamura, Kaoru, Kudo, Toshiaki
The genes responsible for angular dioxygenation of dibenzofuran in actinomycetes were cloned by using a degenerate set of PCR primers designed by using conserved sequences of the dioxygenase alpha...
Noda, Satoko, Ohkuma, Moriya, Yamada, Akinori, Hongoh, Yuichi, Kudo, Toshiaki
Phylogenetic relationships, diversity, and in situ identification of spirochetes in the gut of the termite Neotermes koshunensis were examined without cultivation, with an emphasis on ectosymbionts...
Ohtsubo, Yoshiyuki, Shimura, Minoru, Delawary, Mina, Kimbara, Kazuhide, Takagi, Masamichi, Kudo, Toshiaki, ...
To improve the capabilities of microorganisms relevant for biodegradation, we developed a new genetic approach and applied it to the bph operon (bphEGF[orf4]A1A2A3CD[orf1]A4R) of Pseudomonas sp....
Horinouchi, Masae, Hayashi, Toshiaki, Koshino, Hiroyuki, Yamamoto, Takako, Kudo, Toshiaki
In a previous study we isolated the meta-cleavage enzyme gene, tesB, that encodes an enzyme that carries out a meta-cleavage reaction in the breakdown of testosterone by Comamonas testeroni TA441 (M....
Horinouchi, Masae, Hayashi, Toshiaki, Yamamoto, Takako, Kudo, Toshiaki
In Comamonas testosteroni TA441, testosterone is degraded via aromatization of the A ring, which is cleaved by the meta-cleavage enzyme TesB, and further degraded by TesD, the hydrolase for the...
Characterization of a Protease from a Psychrotroph, Pseudomonas fluorescens 114
Hamamoto, Tetsuo, Kaneda, Motohiro, Horikoshi, Koki, Kudo, Toshiaki
A psychrotrophic bacterium isolated from river sediment was identified as Pseudomonas fluorescens 114. It grew at 0°C and optimally at 20°C. The bacterium produced a protease with a molecular...
Kosono, Saori, Haga, Koki, Tomizawa, Rui, Kajiyama, Yusuke, Hatano, Kazuo, Takeda, Shinobu, ...
Sha (also known as Mrp/Mnh/Pha) is a Na+/H+ antiporter encoded by a cluster of six or seven genes that probably form a multisubunit transport complex. The Sha system is important for the homeostasis...
Horinouchi, Masae, Hayashi, Toshiaki, Koshino, Hiroyuki, Kurita, Tomokazu, Kudo, Toshiaki
Comamonas testosteroni TA441 utilizes testosterone via aromatization of the A ring followed by meta-cleavage of the ring. The product of the meta-cleavage reaction,...
Hongoh, Yuichi, Deevong, Pinsurang, Inoue, Tetsushi, Moriya, Shigeharu, Trakulnaleamsai, Savitr, Ohkuma, Moriya, ...
We investigated the bacterial gut microbiota from 32 colonies of wood-feeding termites, comprising four Microcerotermes species (Termitidae) and four Reticulitermes species (Rhinotermitidae), using...
Taprab, Yaovapa, Johjima, Toru, Maeda, Yoshimasa, Moriya, Shigeharu, Trakulnaleamsai, Savitr, Noparatnaraporn, Napavarn, ...
Fungus-growing termites efficiently decompose plant litter through their symbiotic relationship with basidiomycete fungi of the genus Termitomyces. Here, we investigated phenol-oxidizing enzymes in...
Noda, Satoko, Iida, Toshiya, Kitade, Osamu, Nakajima, Hideaki, Kudo, Toshiaki, Ohkuma, Moriya
A unique lineage of bacteria belonging to the order Bacteroidales was identified as an intracellular endosymbiont of the protist Pseudotrichonympha grassii (Parabasalia, Hypermastigea) in the gut of...
Ohkuma, Moriya, Noda, Satoko, Kudo, Toshiaki
Nitrogen fixation by the microorganisms in the gut of termites is one of the crucial aspects of symbiosis, since termites usually thrive on a nitrogen-poor diet. The phylogenetic diversity of the...
Noda, Satoko, Ohkuma, Moriya, Usami, Ron, Horikoshi, Koki, Kudo, Toshiaki
Expression of the nitrogen fixation gene, nifH, in the gut of the termite Neotermes koshunensis was characterized without cultivation. nifH cDNA was directly amplified from mRNA of the mixed...
Kosono, Saori, Ohashi, Yoshiaki, Kawamura, Fujio, Kitada, Makio, Kudo, Toshiaki
ShaA (sodium/hydrogen antiporter, previously termed YufT [or NtrA]), which is responsible for Na+/H+ antiporter activity, is considered to be the major Na+ excretion system in Bacillus subtilis. We...
Iida, Toshiya, Mukouzaka, Yuki, Nakamura, Kaoru, Kudo, Toshiaki
The genes responsible for angular dioxygenation of dibenzofuran in actinomycetes were cloned by using a degenerate set of PCR primers designed by using conserved sequences of the dioxygenase alpha...
Noda, Satoko, Ohkuma, Moriya, Yamada, Akinori, Hongoh, Yuichi, Kudo, Toshiaki
Phylogenetic relationships, diversity, and in situ identification of spirochetes in the gut of the termite Neotermes koshunensis were examined without cultivation, with an emphasis on ectosymbionts...
Ohtsubo, Yoshiyuki, Shimura, Minoru, Delawary, Mina, Kimbara, Kazuhide, Takagi, Masamichi, Kudo, Toshiaki, ...
To improve the capabilities of microorganisms relevant for biodegradation, we developed a new genetic approach and applied it to the bph operon (bphEGF[orf4]A1A2A3CD[orf1]A4R) of Pseudomonas sp....
Horinouchi, Masae, Hayashi, Toshiaki, Koshino, Hiroyuki, Yamamoto, Takako, Kudo, Toshiaki
In a previous study we isolated the meta-cleavage enzyme gene, tesB, that encodes an enzyme that carries out a meta-cleavage reaction in the breakdown of testosterone by Comamonas testeroni TA441 (M....
Horinouchi, Masae, Hayashi, Toshiaki, Yamamoto, Takako, Kudo, Toshiaki
In Comamonas testosteroni TA441, testosterone is degraded via aromatization of the A ring, which is cleaved by the meta-cleavage enzyme TesB, and further degraded by TesD, the hydrolase for the...
Characterization of a Protease from a Psychrotroph, Pseudomonas fluorescens 114
Hamamoto, Tetsuo, Kaneda, Motohiro, Horikoshi, Koki, Kudo, Toshiaki
A psychrotrophic bacterium isolated from river sediment was identified as Pseudomonas fluorescens 114. It grew at 0°C and optimally at 20°C. The bacterium produced a protease with a molecular...
Nakabachi, Atsushi, Shigenobu, Shuji, Sakazume, Naoko, Shiraki, Toshiyuki, Hayashizaki, Yoshihide, Carninci, Piero, ...
Aphids possess bacteriocytes, cells specifically differentiated to harbor obligatory mutualistic bacteria of the genus Buchnera, which have lost many genes that are essential for common bacterial...
Kosono, Saori, Haga, Koki, Tomizawa, Rui, Kajiyama, Yusuke, Hatano, Kazuo, Takeda, Shinobu, ...
Sha (also known as Mrp/Mnh/Pha) is a Na+/H+ antiporter encoded by a cluster of six or seven genes that probably form a multisubunit transport complex. The Sha system is important for the homeostasis...
Horinouchi, Masae, Hayashi, Toshiaki, Koshino, Hiroyuki, Kurita, Tomokazu, Kudo, Toshiaki
Comamonas testosteroni TA441 utilizes testosterone via aromatization of the A ring followed by meta-cleavage of the ring. The product of the meta-cleavage reaction,...
Hongoh, Yuichi, Deevong, Pinsurang, Inoue, Tetsushi, Moriya, Shigeharu, Trakulnaleamsai, Savitr, Ohkuma, Moriya, ...
We investigated the bacterial gut microbiota from 32 colonies of wood-feeding termites, comprising four Microcerotermes species (Termitidae) and four Reticulitermes species (Rhinotermitidae), using...
Taprab, Yaovapa, Johjima, Toru, Maeda, Yoshimasa, Moriya, Shigeharu, Trakulnaleamsai, Savitr, Noparatnaraporn, Napavarn, ...
Fungus-growing termites efficiently decompose plant litter through their symbiotic relationship with basidiomycete fungi of the genus Termitomyces. Here, we investigated phenol-oxidizing enzymes in...
Noda, Satoko, Iida, Toshiya, Kitade, Osamu, Nakajima, Hideaki, Kudo, Toshiaki, Ohkuma, Moriya
A unique lineage of bacteria belonging to the order Bacteroidales was identified as an intracellular endosymbiont of the protist Pseudotrichonympha grassii (Parabasalia, Hypermastigea) in the gut of...
Hongoh, Yuichi, Deevong, Pinsurang, Hattori, Satoshi, Inoue, Tetsushi, Noda, Satoko, Noparatnaraporn, Napavarn, ...
Recently we discovered two novel, deeply branching lineages in the domain Bacteria from termite guts by PCR-based analyses of 16S rRNA (Y. Hongoh, P. Deevong, T. Inoue, S. Moriya, S. Trakulnaleamsai,...
Igawa, Tomoko, Takahashi-Ando, Naoko, Ochiai, Noriyuki, Ohsato, Shuichi, Shimizu, Tsutomu, Kudo, Toshiaki, ...
Maize is subject to ear rot caused by toxigenic Aspergillus and Fusarium species, resulting in contamination with aflatoxins, fumonisins, trichothecenes, and zearalenone (ZEN). The trichothecene...
The Antifungal Polyketide Ambruticin Targets the HOG Pathway▿
Vetcher, Leandro, Menzella, Hugo G., Kudo, Toshiaki, Motoyama, Takayuki, Katz, Leonard
The polyketide ambruticin is an attractive candidate for drug development as an antifungal agent, but its mechanism of action has not yet been elucidated. Here we present evidence that ambruticin...
Inoue, Jun-Ichi, Saita, Kanako, Kudo, Toshiaki, Ui, Sadaharu, Ohkuma, Moriya
Cellulolytic flagellated protists in the guts of termites produce molecular hydrogen (H2) that is emitted by the termites; however, little is known about the physiology and biochemistry of H2...
Hongoh, Yuichi, Sato, Tomoyuki, Dolan, Michael F., Noda, Satoko, Ui, Sadaharu, Kudo, Toshiaki, ...
The flagellate Caduceia versatilis in the gut of the termite Cryptotermes cavifrons reportedly propels itself not by its own flagella but solely by the flagella of ectosymbiotic bacteria. Previous...
Kajiyama, Yusuke, Otagiri, Masato, Sekiguchi, Junichi, Kosono, Saori, Kudo, Toshiaki
The Bacillus subtilis Mrp (also referred to as Sha) is a particularly unusual Na+/H+ antiporter encoded by mrpABCDEFG. Using His tagging of Mrp proteins, we showed complex formation by the mrpABCDEFG...
Complete genome of the uncultured Termite Group 1 bacteria in a single host protist cell
Hongoh, Yuichi, Sharma, Vineet K., Prakash, Tulika, Noda, Satoko, Taylor, Todd D., Kudo, Toshiaki, ...
Termites harbor a symbiotic gut microbial community that is responsible for their ability to thrive on recalcitrant plant matter. The community comprises diverse microorganisms, most of which are as...
Horinouchi, Masae, Hayashi, Toshiaki, Koshino, Hiroyuki, Malon, Michal, Yamamoto, Takako, Kudo, Toshiaki
Comamonas testosteroni TA441 degrades steroids such as testosterone via aromatization of the A ring, followed by meta-cleavage of the ring. In the DNA region upstream of the meta-cleavage enzyme gene...
Yasuda, Michiko, Ishikawa, Atsushi, Jikumaru, Yusuke, Seki, Motoaki, Umezawa, Taishi, Asami, Tadao, ...
Systemic acquired resistance (SAR) is a potent innate immunity system in plants that is effective against a broad range of pathogens. SAR development in dicotyledonous plants, such as tobacco...
Iida, Toshiya, Waki, Taro, Nakamura, Kaoru, Mukouzaka, Yuki, Kudo, Toshiaki
Dibenzofuran (DF) is one of the dioxin carbon skeletal compounds used as a model to study the microbial degradation of dioxins. This study analyzed the transcriptional regulation of the DF...