Toshio Joh

Molecular Cloning and Expression of Hardening-Induced Genes in Chlorella vulgaris C-27: the Most Abundant Clone Encodes a Late Embryogenesis Abundant Protein (1995)

Joh, Toshio, Honjoh, Ken-ichi, Yoshimoto, Makoto, Funabashi, Jun, Miyamoto, Takahisa, Hatano, Shoji

To investigate the effects of hardening on gene expression in Chlorella vulgaris Beijerink IAM C-27 (formerly Chlorella ellipsoidea Gerneck IAM C-27), a frost-hardy strain, 17 cDNA clones...

Isolation and Characterization of Hardening-Induced Proteins in Chlorella vulgaris C-27: Identification of Late Embryogenesis Abundant Proteins (1995)

Honjoh, Ken-ichi, Yoshimoto, Makoto, Joh, Toshio, Kajiwara, Taishin, Miyamoto, Takahisa, Hatano, Shoji

Hardening-induced soluble proteins of Chlorella vulgaris Beijerink IAM C-27 (formerly Chlorella ellipsoidea Gerneck IAM C-27) were isolated and purified by two-dimensional high-performance liquid...

Preparation of Protoplasts from Chiorella ellipsoidea C-27 (1992)

Hatano, Shoji, Joh, Toshio, Miyamoto, Takahisa, Yoshimoto, Makoto

Protoplasts from Chlorella ellipsoidea IAM C-27 were rapidly obtained by enzymatic digestion with a mixture of Chitosanase KI, mixed glycosidases, and a cell wall-lytic enzyme found in the cell...