Tamura, Toshiki, Ariga, Haruyuki, Kinashi, Tatsuo, Uehara, Shuichiro, Kikuchi, Takeshi, Nakada, Makiyo, ...
CD4+ Th1 cells play a critical role in the induction of cell-mediated immune responses that are important for the eradication of intracellular pathogens. Peptide-25 is the major Th1 epitope for...
Negamycin Restores Dystrophin Expression in Skeletal and Cardiac Muscles of mdx Mice (2003)
Arakawa, Masayuki, Shiozuka, Masataka, Nakayama, Yuki, Hara, Takahiko, Hamada, Masa, Kondo, Shinichi, ...
The ability of aminoglycoside antibiotics to promote read-through of nonsense mutations has attracted interest in these drugs as potential therapeutic agents in genetic diseases. However, the...
Bao, Ying-Chun, Tsuruga, Hiromichi, Hirai, Momoki, Yasuda, Kazuki, Yokoi, Norihide, Kitamura, Toshio, ...
We report the cloning and characterization of a human cDNA predicted to encode a novel hydrophobic protein containing four transmembrane domains and a zinc metalloprotease motif, HEXXH, between the...
Kawamura, Yuuki, Kokame, Koichi, Yamaguchi, Haruyasu, Shiraishi, Hirohisa, Suzuki, Ryo, ...
Presenilin (PS) is essential for the gamma-cleavage required for the generation of the C terminus of amyloid beta-protein (Abeta). However, the mechanism underlying PS-mediated gamma-cleavage remains...
Mukasa, Ryuta, Homma, Toshio, Ohtsuki, Takashi, Hosono, Osamu, Souta, Akiko, Kitamura, Toshio, ...
Human CD4 T cells can be divided into two functionally distinct subsets: a CD45RO+ memory subset and a CD45RA+ naive subset. In an attempt to identify novel cell surface molecules on these...
Kitamura, Toshio, Takaku, Fumimaro, Miyajlma, Atsushi, Miyasaka, M.
A factor-dependent human hemopoietic cell line, TF-1, requires interleukln 3 (IL-3) or granulocyte/acrophage colony-stimulating factor (GM-CSF) for its long-term growth. We have found that IL-4,...
A method to identify cDNAs based on localization of green fluorescent protein fusion products
Misawa, Kazuhide, Nosaka, Tetsuya, Morita, Sumiyo, Kaneko, Azusa, Nakahata, Tatsutoshi, Asano, Shigetaka, ...
We previously established a high-efficiency, retrovirus-mediated expression cloning method. Using this system, we now have developed an expression cloning method (FL-REX; fluorescence...
Bruton’s tyrosine kinase regulates apoptosis and JNK/SAPK kinase activity
Kawakami, Yuko, Miura, Toru, Bissonnette, Reid, Hata, Daisuke, Khan, Wasif N., Kitamura, Toshio, ...
Mast cells derived from Bruton’s tyrosine kinase (Btk)-defective xid or btk null mice showed greater expansion in culture containing interleukin-3 (IL-3) than those from wild-type (wt) mice....
Onishi, Mayumi, Nosaka, Tetsuya, Misawa, Kazuhide, Mui, Alice L.-F., Gorman, Daniel, McMahon, Martin, ...
STAT (signal transducers and activators of transcription) proteins are transcription factors which are activated by phosphorylation on tyrosine residues upon stimulation by cytokines. Seven members...
Shimizu, Nobuaki, Soda, Yasushi, Kanbe, Katsuaki, Liu, Hui-yu, Mukai, Ryozaburo, Kitamura, Toshio, ...
More than 10 G protein-coupled receptors (GPCRs) have been shown to act as coreceptors for infection of human immunodeficiency virus type 1 (HIV-1), HIV-2, and simian immunodeficiency virus (SIV). We...
Shimizu, Nobuaki, Soda, Yasushi, Kanbe, Katsuaki, Liu, Hui-Yu, Jinno, Atsushi, Kitamura, Toshio, ...
Twelve G protein-coupled receptors, including chemokine receptors, act as coreceptors and determinants for the cell tropisms of human immunodeficiency virus type 1 (HIV-1), HIV-2, and simian...
Functional cloning of BRF1, a regulator of ARE-dependent mRNA turnover
Stoecklin, Georg, Colombi, Marco, Raineri, Ines, Leuenberger, Sabrina, Mallaun, Michel, Schmidlin, Martin, ...
To identify regulators of AU-rich element (ARE)-dependent mRNA turnover we have followed a genetic approach using a mutagenized cell line (slowC) that fails to degrade cytokine mRNA. Accordingly, a...
Mammalian Twisted Gastrulation Is Essential for Skeleto-Lymphogenesis
Nosaka, Tetsuya, Morita, Sumiyo, Kitamura, Hidetomo, Nakajima, Hideaki, Shibata, Fumi, Morikawa, Yoshihiro, ...
Dorsoventral patterning depends on the local concentrations of the morphogens. Twisted gastrulation (TSG) regulates the extracellular availability of a mesoderm inducer, bone morphogenetic protein 4...
Ohtsuka, Makoto, Arase, Hisashi, Takeuchi, Arata, Yamasaki, Sho, Shiina, Ritsuko, Suenaga, Tadahiro, ...
A functional cDNA cloning system was developed by using a retrovirus library encoding CD8-chimeric proteins and a nuclear factor of activated T cells (NFAT)-GFP reporter cell line to identify...
Ikeda, Yuichi, Imai, Yasushi, Kumagai, Hidetoshi, Nosaka, Tetsuya, Morikawa, Yoshihiro, Hisaoka, Tomoko, ...
Growth factors, cell-surface receptors, adhesion molecules, and extracellular matrix proteins play critical roles in vascular pathophysiology by affecting growth, migration, differentiation, and...
Ono, Ryoichi, Nakajima, Hideaki, Ozaki, Katsutoshi, Kumagai, Hidetoshi, Kawashima, Toshiyuki, Taki, Tomohiko, ...
The mechanisms by which mixed-lineage leukemia (MLL) fusion products resulting from in utero translocations in 11q23 contribute to leukemogenesis and infant acute leukemia remain elusive. It is still...
Urano, Atsushi, Endoh, Masaki, Wada, Tadashi, Morikawa, Yoshihiro, Itoh, Miyuki, Kataoka, Yuki, ...
AF5q31 (also called MCEF) was identified by its involvement in chromosomal translocation with the gene MLL (mixed lineage leukemia), which is associated with infant acute lymphoblastic leukemia....
The Constitutive Centromere Component CENP-50 Is Required for Recovery from Spindle Damage‡
Minoshima, Yukinori, Hori, Tetsuya, Okada, Masahiro, Kimura, Hiroshi, Haraguchi, Tokuko, Hiraoka, Yasushi, ...
We identified CENP-50 as a novel kinetochore component. We found that CENP-50 is a constitutive component of the centromere that colocalizes with CENP-A and CENP-H throughout the cell cycle in...
Ono, Ryoichi, Ihara, Masafumi, Nakajima, Hideaki, Ozaki, Katsutoshi, Kataoka-Fujiwara, Yuki, Taki, Tomohiko, ...
Septins are evolutionarily conserved GTP-binding proteins that can heteropolymerize into filaments. Recent studies have revealed that septins are involved in not only diverse normal cellular...
A method to identify cDNAs based on localization of green fluorescent protein fusion products
Misawa, Kazuhide, Nosaka, Tetsuya, Morita, Sumiyo, Kaneko, Azusa, Nakahata, Tatsutoshi, Asano, Shigetaka, ...
We previously established a high-efficiency, retrovirus-mediated expression cloning method. Using this system, we now have developed an expression cloning method (FL-REX; fluorescence...
Bruton’s tyrosine kinase regulates apoptosis and JNK/SAPK kinase activity
Kawakami, Yuko, Miura, Toru, Bissonnette, Reid, Hata, Daisuke, Khan, Wasif N., Kitamura, Toshio, ...
Mast cells derived from Bruton’s tyrosine kinase (Btk)-defective xid or btk null mice showed greater expansion in culture containing interleukin-3 (IL-3) than those from wild-type (wt) mice....
Onishi, Mayumi, Nosaka, Tetsuya, Misawa, Kazuhide, Mui, Alice L.-F., Gorman, Daniel, McMahon, Martin, ...
STAT (signal transducers and activators of transcription) proteins are transcription factors which are activated by phosphorylation on tyrosine residues upon stimulation by cytokines. Seven members...
Shimizu, Nobuaki, Soda, Yasushi, Kanbe, Katsuaki, Liu, Hui-yu, Mukai, Ryozaburo, Kitamura, Toshio, ...
More than 10 G protein-coupled receptors (GPCRs) have been shown to act as coreceptors for infection of human immunodeficiency virus type 1 (HIV-1), HIV-2, and simian immunodeficiency virus (SIV). We...
Shimizu, Nobuaki, Soda, Yasushi, Kanbe, Katsuaki, Liu, Hui-Yu, Jinno, Atsushi, Kitamura, Toshio, ...
Twelve G protein-coupled receptors, including chemokine receptors, act as coreceptors and determinants for the cell tropisms of human immunodeficiency virus type 1 (HIV-1), HIV-2, and simian...
Functional cloning of BRF1, a regulator of ARE-dependent mRNA turnover
Stoecklin, Georg, Colombi, Marco, Raineri, Ines, Leuenberger, Sabrina, Mallaun, Michel, Schmidlin, Martin, ...
To identify regulators of AU-rich element (ARE)-dependent mRNA turnover we have followed a genetic approach using a mutagenized cell line (slowC) that fails to degrade cytokine mRNA. Accordingly, a...
Mammalian Twisted Gastrulation Is Essential for Skeleto-Lymphogenesis
Nosaka, Tetsuya, Morita, Sumiyo, Kitamura, Hidetomo, Nakajima, Hideaki, Shibata, Fumi, Morikawa, Yoshihiro, ...
Dorsoventral patterning depends on the local concentrations of the morphogens. Twisted gastrulation (TSG) regulates the extracellular availability of a mesoderm inducer, bone morphogenetic protein 4...
Ohtsuka, Makoto, Arase, Hisashi, Takeuchi, Arata, Yamasaki, Sho, Shiina, Ritsuko, Suenaga, Tadahiro, ...
A functional cDNA cloning system was developed by using a retrovirus library encoding CD8-chimeric proteins and a nuclear factor of activated T cells (NFAT)-GFP reporter cell line to identify...
Ikeda, Yuichi, Imai, Yasushi, Kumagai, Hidetoshi, Nosaka, Tetsuya, Morikawa, Yoshihiro, Hisaoka, Tomoko, ...
Growth factors, cell-surface receptors, adhesion molecules, and extracellular matrix proteins play critical roles in vascular pathophysiology by affecting growth, migration, differentiation, and...
Ono, Ryoichi, Nakajima, Hideaki, Ozaki, Katsutoshi, Kumagai, Hidetoshi, Kawashima, Toshiyuki, Taki, Tomohiko, ...
The mechanisms by which mixed-lineage leukemia (MLL) fusion products resulting from in utero translocations in 11q23 contribute to leukemogenesis and infant acute leukemia remain elusive. It is still...
Urano, Atsushi, Endoh, Masaki, Wada, Tadashi, Morikawa, Yoshihiro, Itoh, Miyuki, Kataoka, Yuki, ...
AF5q31 (also called MCEF) was identified by its involvement in chromosomal translocation with the gene MLL (mixed lineage leukemia), which is associated with infant acute lymphoblastic leukemia....
The Constitutive Centromere Component CENP-50 Is Required for Recovery from Spindle Damage‡
Minoshima, Yukinori, Hori, Tetsuya, Okada, Masahiro, Kimura, Hiroshi, Haraguchi, Tokuko, Hiraoka, Yasushi, ...
We identified CENP-50 as a novel kinetochore component. We found that CENP-50 is a constitutive component of the centromere that colocalizes with CENP-A and CENP-H throughout the cell cycle in...
Ono, Ryoichi, Ihara, Masafumi, Nakajima, Hideaki, Ozaki, Katsutoshi, Kataoka-Fujiwara, Yuki, Taki, Tomohiko, ...
Septins are evolutionarily conserved GTP-binding proteins that can heteropolymerize into filaments. Recent studies have revealed that septins are involved in not only diverse normal cellular...
Nakayama, Yuki, Nara, Noriko, Kawakita, Yukiko, Takeshima, Yasuhiro, Arakawa, Masayuki, Katoh, Masaki, ...
In the dystrophin-mutant mdx mouse, an animal model for Duchenne muscular dystrophy (DMD), damaged skeletal muscles are efficiently regenerated and thus the animals thrive. The phenotypic differences...
Helicobacter cinaedi Cellulitis and Bacteremia in Immunocompetent Hosts after Orthopedic Surgery▿
Kitamura, Toshio, Kawamura, Yoshiaki, Ohkusu, Kiyofumi, Masaki, Takayuki, Iwashita, Hirofumi, Sawa, Tomohiro, ...
At various times after orthopedic operations (more than a few weeks, with an average of 29.9 days), 11 patients had a sudden onset of high temperature (average 38.9°C) and local cellulitis at...
Fukuchi, Yumi, Shibata, Fumi, Ito, Miyuki, Goto-Koshino, Yuko, Sotomaru, Yusuke, Ito, Mamoru, ...
CCAAT/enhancer-binding protein (C/EBP) α is a critical regulator for early myeloid differentiation. Although C/EBPα has been shown to convert B cells into myeloid lineage, precise roles of C/EBPα...
Kawashima, Toshiyuki, Bao, Ying Chun, Nomura, Yasushi, Moon, Yuseok, Tonozuka, Yukio, Minoshima, Yukinori, ...
STAT transcription factors are tyrosine phosphorylated upon cytokine stimulation and enter the nucleus to activate target genes. We show that Rac1 and a GTPase-activating protein, MgcRacGAP, bind...
Ect2 and MgcRacGAP regulate the activation and function of Cdc42 in mitosis
Oceguera-Yanez, Fabian, Kimura, Kazuhiro, Yasuda, Shingo, Higashida, Chiharu, Kitamura, Toshio, Hiraoka, Yasushi, ...
Although Rho regulates cytokinesis, little was known about the functions in mitosis of Cdc42 and Rac. We recently suggested that Cdc42 works in metaphase by regulating bi-orient attachment of spindle...
Kato, Yuko, Iwama, Atsushi, Tadokoro, Yuko, Shimoda, Kazuya, Minoguchi, Mayu, Akira, Shizuo, ...
Although the concept of a leukemic stem cell system has recently been well accepted, its nature and the underlying molecular mechanisms remain obscure. Constitutive activation of signal transducers...
Endomucin, a CD34-like sialomucin, marks hematopoietic stem cells throughout development
Matsubara, Azusa, Iwama, Atsushi, Yamazaki, Satoshi, Furuta, Chie, Hirasawa, Ryutaro, Morita, Yohei, ...
To detect as yet unidentified cell-surface molecules specific to hematopoietic stem cells (HSCs), a modified signal sequence trap was successfully applied to mouse bone marrow (BM)...
Nieborowska-Skorska, Malgorzata, Wasik, Mariusz A., Slupianek, Artur, Salomoni, Paolo, Kitamura, Toshio, Calabretta, Bruno, ...
Signal transducer and activator of transcription (STAT)5 is constitutively activated in BCR/ ABL-expressing cells, but the mechanisms and functional consequences of such activation are unknown. We...
Hata, Daisuke, Kawakami, Yuko, Inagaki, Naoki, Lantz, Chris S., Kitamura, Toshio, Khan, Wasif N., ...
We investigated the role of Bruton's tyrosine kinase (Btk) in FcεRI-dependent activation of mouse mast cells, using xid and btk null mutant mice. Unlike B cell development, mast cell development is...
Gugasyan, Raffi, Quilici, Cathy, I, Stacey T.T., Grail, Dianne, Verhagen, Anne M., Roberts, Andrew, ...
Downstream of kinase (Dok)–related protein (DokR, also known as p56dok/FRIP/Dok-R) is implicated in cytokine and immunoreceptor signaling in myeloid and T cells. Tyrosine phosphorylation induces...
Iwashita, Hirofumi, Fujii, Shigemoto, Kawamura, Yoshiaki, Okamoto, Tatsuya, Sawa, Tomohiro, Masaki, Takayuki, ...
Helicobacter cinaedi infection is now recognized as an increasingly important emerging disease. Its pathogenesis and epidemiological features are not fully understood, however. Here, we investigated...
A Simple Detection Method for Low-Affinity Membrane Protein Interactions by Baculoviral Display
Sakihama, Toshiko, Sato, Takato, Iwanari, Hiroko, Kitamura, Toshio, Sakaguchi, Shimon, Kodama, Tatsuhiko, ...
Sekine, Rieko, Kitamura, Toshio, Tsuji, Takashi, Tojo, Arinobu
We applied a coculture system for the genetic manipulation of human B-lymphoid and myeloid progenitor cells using murine bone marrow stromal cell support, and investigated the effects of forced Pax5...
Kawashima, Toshiyuki, Bao, Ying Chun, Minoshima, Yukinori, Nomura, Yasushi, Hatori, Tomonori, Hori, Tetsuya, ...
In addition to their pleiotropic functions under physiological conditions, transcription factors STAT3 and STAT5 also have oncogenic activities, but how activated STATs are transported to the nucleus...