Toshio Ohtani

Comparative structural biology of the genome: nano-scale imaging of single nucleus from different kingdoms reveals the common physicochemical property of chromatin with a 40 nm structural unit (2006)

Kobori, Toshiro, Kodama, Mami, Hizume, Kohji, Yoshimura, Shige H., Ohtani, Toshio, Takeyasu, Kunio

Genome function is closely linked to the higher-order chromatin structures. To reveal a structural basis for the interphase chromatin organization, the ‘on-substrate’ lysis procedure was applied...

Comparative structural biology of the genome: nano-scale imaging of single nucleus from different kingdoms reveals the common physicochemical property of chromatin with a 40 nm structural unit (2006)

Kobori, Toshiro, Kodama, Mami, Hizume, Kohji, Yoshimura, Shige H., Ohtani, Toshio, Takeyasu, Kunio

Genome function is closely linked to the higher-order chromatin structures. To reveal a structural basis for the interphase chromatin organization, the ‘on-substrate’ lysis procedure was applied...

Comparative structural biology of the genome: nano-scale imaging of single nucleus from different kingdoms reveals the common physicochemical property of chromatin with a 40 nm structural unit (2006)

Kobori, Toshiro, Kodama, Mami, Hizume, Kohji, Yoshimura, Shige H., Ohtani, Toshio, Takeyasu, Kunio

Genome function is closely linked to the higher-order chromatin structures. To reveal a structural basis for the interphase chromatin organization, the ‘on-substrate’ lysis procedure was applied...

Direct Visualization of Abasic Sites on a Single DNA Molecule Using Fluorescence Microscopy¶ (2002)

Tamaki Hirose, Toshio Ohtani, Hiroshi Muramatsu, Atsushi Tanaka

A new method was developed to allow direct visualization of damaged sites on individual DNA molecules. Fluorescence in situ hybridization on extended DNA molecules was modified to detect a single...

Useful technique for DNA-stretching and fixation (2002)

Nakao, Hidenobu, Hayashi, Hideki, Yoshino, Tomoyuki, Sugiyama, Shigeru, Otobe, Kazunori, Ohtani, Toshio

We report on a useful technique for reproducibly straightening and fixing DNA molecules. When a droplet of DNA solution on surfaces is sucked up by pipet, surface tension at the moving air-water...

Simultaneous collection of topographic and fluorescent images of barley chromosomes by scanning near-field optical/atomic force microscopy (2002)

Yoshino, Tomoyuki, Sugiyama, Shigeru, Hagiwara, Shoji, Ushiki, Tatsuo, Ohtani, Toshio

The present study investigated the applicability of scanning near‐field optical/atomic force microscopy (SNOM/AFM) to observations of YOYO‐1 stained barley chromosomes. Using...

Behavior of DNA fibers stretched by precise meniscus motion control (2001)

Otobe, Kazunori, Ohtani, Toshio

A modified DNA combing method, which can precisely locate straightened DNA fibers on a substrate, has been developed. Precise motion control of a DNA solution droplet on hydrophobic surfaces has...

Observations of xenon gas-treated barley cells in solution by atomic force microscopy (2000)

Yoshino, Tomoyuki, Sotome, Itaru, Ohtani, Toshio, Isobe, Seiichiro, Oshita, Sei-ichi, Maekawa, Takaaki

Barley cells cut from a sprout were exposed to either air or high-pressure xenon gas for 3 days and the surface of those cells was observed by atomic force microscopy (AFM) to examine the effect of...

Structure of rice starch granules in nanometre scale as revealed by atomic force microscopy (2000)

Ohtani, Toshio, Yoshino, Tomoyuki, Ushiki, Tatsuo, Hagiwara, Shouji, Maekawa, Takaaki

Atomic force microscopy (AFM) was performed for the analysis of the fine structure of rice starch granules in the nanometre scale which were prepared by a physical destruction method. The present...

Behavior of DNA fibers stretched by precise meniscus motion control

Otobe, Kazunori, Ohtani, Toshio

A modified DNA combing method, which can precisely locate straightened DNA fibers on a substrate, has been developed. Precise motion control of a DNA solution droplet on hydrophobic surfaces has...

Difference in Bacterial Motion between Forward and Backward Swimming Caused by the Wall Effect

Magariyama, Yukio, Ichiba, Makoto, Nakata, Kousou, Baba, Kensaku, Ohtani, Toshio, Kudo, Seishi, ...

A bacterial cell that has a single polar flagellum alternately repeats forward swimming, in which the flagellum pushes the cell body, and backward swimming, in which the flagellum pulls the cell...

Behavior of DNA fibers stretched by precise meniscus motion control

Otobe, Kazunori, Ohtani, Toshio

A modified DNA combing method, which can precisely locate straightened DNA fibers on a substrate, has been developed. Precise motion control of a DNA solution droplet on hydrophobic surfaces has...

Difference in Bacterial Motion between Forward and Backward Swimming Caused by the Wall Effect

Magariyama, Yukio, Ichiba, Makoto, Nakata, Kousou, Baba, Kensaku, Ohtani, Toshio, Kudo, Seishi, ...

A bacterial cell that has a single polar flagellum alternately repeats forward swimming, in which the flagellum pushes the cell body, and backward swimming, in which the flagellum pulls the cell...