The Protein Import Channel in the Outer Mitosomal Membrane of Giardia intestinalis (2009)
Dagley, Michael J., Dolezal, Pavel, Likic, Vladimir A., Smid, Ondrej, Purcell, Anthony W., Buchanan, Susan K., ...
The identification of mitosomes in Giardia generated significant debate on the evolutionary origin of these organelles, whether they were highly reduced mitochondria or the product of a unique...
Pusnik, Mascha, Charrière, Fabien, Mäser, Pascal, Waller, Ross F., Dagley, Michael J., Lithgow, Trevor, ...
All mitochondria have integral outer membrane proteins with β-barrel structures including the conserved metabolite transporter VDAC (voltage dependent anion channel) and the conserved protein import...
Schneider, André, Bursać, Dejan, Lithgow, Trevor
Trypanosoma brucei is a unicellular eukaryote that causes the deadly human African trypanosomiasis (‘sleeping sickness’) in humans. The parasite has a complicated lifestyle, it developmentally...
Schneider, André, Bursać, Dejan, Lithgow, Trevor
Trypanosoma brucei is a unicellular eukaryote that causes the deadly human African trypanosomiasis (‘sleeping sickness’) in humans. The parasite has a complicated lifestyle, it developmentally...
Gentle, Ian E., Perry, Andrew J., Alcock, Felicity H., Likic, Vladimir A., Dolezal, Pavel, Ng, Ee Ting, ...
The mitochondrial inner and outer membranes are composed of a variety of integral membrane proteins, assembled into the membranes posttranslationally. The small translocase of the inner mitochondrial...
Gentle, Ian E., Perry, Andrew J., Alcock, Felicity H., Likic, Vladimir A., Dolezal, Pavel, Ng, Ee Ting, ...
The mitochondrial inner and outer membranes are composed of a variety of integral membrane proteins, assembled into the membranes posttranslationally. The small translocase of the inner mitochondrial...
Domain Stealing by Receptors in a Protein Transport Complex (2007)
Hulett, Joanne M., Walsh, Peter, Lithgow, Trevor
The mitochondrion is an essential cellular compartment in eukaryotes. The mitochondrial proteins Tom20 and Tom22 are receptors that ensure recognition and binding of proteins imported for...
Gentle, Ian E., Perry, Andrew J., Alcock, Felicity H., Likic, Vladimir A., Dolezal, Pavel, Ng, Ee Ting, ...
The mitochondrial inner and outer membranes are composed of a variety of integral membrane proteins, assembled into the membranes posttranslationally. The small translocase of the inner mitochondrial...
Gentle, Ian E., Perry, Andrew J., Alcock, Felicity H., Likic, Vladimir A., Dolezal, Pavel, Ng, Ee Ting, ...
The mitochondrial inner and outer membranes are composed of a variety of integral membrane proteins, assembled into the membranes post-translationally. The small TIMs are a group of ∼10 kDa...
Gentle, Ian, Gabriel, Kipros, Beech, Peter, Waller, Ross, Lithgow, Trevor
Integral proteins in the outer membrane of mitochondria control all aspects of organelle biogenesis, being required for protein import, mitochondrial fission, and, in metazoans, mitochondrial aspects...
Bacterial Proteins Predisposed for Targeting to Mitochondria (2004)
Lucattini, Rebecca, Liki, Vladimir A., Lithgow, Trevor
Mitochondria evolved from an endosymbiotic proteobacterium in a process that required the transfer of genes from the bacterium to the host cell nucleus and the translocation of proteins thereby made...
Maaev, Diana, Whelan, James, Newbigin, Ed, Silva-Filho, Marcio C., Mulhern, Terrence D., Lithgow, Trevor
One of the earliest events in the evolution of mitochondria was to devise a means to translocate proteins made in the cytosol into the "proto-mitochondrion". How this was achieved remains uncertain...
Bacterial Proteins Predisposed for Targeting to Mitochondria (2004)
Lucattini, Rebecca, Likic, Vladimir A., Lithgow, Trevor
Mitochondria evolved from an endosymbiotic proteobacterium in a process that required the transfer of genes from the bacterium to the host cell nucleus, and the translocation of proteins thereby made...
Macasev, Diana, Whelan, James, Newbigin, Ed, Silva-Filho, Marcio C., Mulhern, Terrence D., Lithgow, Trevor
One of the earliest events in the evolution of mitochondria was the development a means to translocate proteins made in the cytosol into the “protomitochondrion.” How this was achieved remains...
Gentle, Ian, Gabriel, Kipros, Beech, Peter, Waller, Ross, Lithgow, Trevor
Integral proteins in the outer membrane of mitochondria control all aspects of organelle biogenesis, being required for protein import, mitochondrial fission, and, in metazoans, mitochondrial aspects...
Bacterial Proteins Predisposed for Targeting to Mitochondria (2004)
Lucattini, Rebecca, Liki, Vladimir A., Lithgow, Trevor
Mitochondria evolved from an endosymbiotic proteobacterium in a process that required the transfer of genes from the bacterium to the host cell nucleus and the translocation of proteins thereby made...
Maaev, Diana, Whelan, James, Newbigin, Ed, Silva-Filho, Marcio C., Mulhern, Terrence D., Lithgow, Trevor
One of the earliest events in the evolution of mitochondria was to devise a means to translocate proteins made in the cytosol into the "proto-mitochondrion". How this was achieved remains uncertain...
Protein structure and function by the sea (2002)
A report on the 27th Annual Lorne Conference on Protein Structure and Function, Lorne, Australia, 9-13 February 2002.
Role for yeast inhibitor of apoptosis (IAP)-like proteins in cell division
Uren, Anthony G., Beilharz, Traude, O’Connell, Matthew J., Bugg, Sarah J., Van Driel, Rosemary, Vaux, David L., ...
Inhibitors of apoptosis (IAPs) are a family of proteins that bear baculoviral IAP repeats (BIRs) and regulate apoptosis in vertebrates and Drosophila melanogaster. The yeasts Saccharomyces cerevisiae...
The yeast nascent polypeptide-associated complex initiates protein targeting to mitochondria in vivo
George, Rebecca, Beddoe, Travis, Landl, Karina, Lithgow, Trevor
The yeast nascent polypeptide-associated complex (NAC) is encoded by two genes, EGD1 and EGD2, and is associated with cytoplasmic ribosomes. Yeast mutants lacking NAC (Δegd2) are viable but suffer...
RAC, a stable ribosome-associated complex in yeast formed by the DnaK-DnaJ homologs Ssz1p and zuotin
Gautschi, Matthias, Lilie, Hauke, Fünfschilling, Ursula, Mun, Andrej, Ross, Suzanne, Lithgow, Trevor, ...
The yeast cytosol contains multiple homologs of the DnaK and DnaJ chaperone family. Our current understanding of which homologs functionally interact is incomplete. Zuotin is a DnaJ homolog bound to...
Protein structure and function by the sea
A report on the 27th Annual Lorne Conference on Protein Structure and Function, Lorne, Australia, 9-13 February 2002.
Gabriel, Kipros, Egan, Billie, Lithgow, Trevor
The Translocase of the Outer Mitochondrial membrane (TOM complex) is centred on a channel, created by Tom40, serving as the only means of entry for proteins into the mitochondrion. Proteins destined...
A SNARE required for retrograde transport to the endoplasmic reticulum
Burri, Lena, Varlamov, Oleg, Doege, Claudia A., Hofmann, Kay, Beilharz, Traude, Rothman, James E., ...
SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) are central components of the machinery mediating membrane fusion in all eukaryotic cells. Sequence analysis of the...
Chávez, Sebastián, Beilharz, Traude, Rondón, Ana G., Erdjument-Bromage, Hediye, Tempst, Paul, Svejstrup, Jesper Q., ...
Transcription-induced recombination has been reported in all organisms from bacteria to mammals. We have shown previously that the yeast genes HPR1 and THO2 may be keys to the understanding of...
Youker, Robert T., Walsh, Peter, Beilharz, Traude, Lithgow, Trevor, Brodsky, Jeffrey L.
Aberrant secreted proteins can be destroyed by ER-associated protein degradation (ERAD), and a prominent, medically relevant ERAD substrate is the cystic fibrosis transmembrane conductance regulator...
Mature DIABLO/Smac Is Produced by the IMP Protease Complex on the Mitochondrial Inner Membrane
Burri, Lena, Strahm, Yvan, Hawkins, Christine J., Gentle, Ian E., Puryer, Michelle A., Verhagen, Anne, ...
DIABLO/Smac is a mitochondrial protein that can promote apoptosis by promoting the release and activation of caspases. To do so, DIABLO/Smac must first be processed by a mitochondrial protease and...
Giardia mitosomes and trichomonad hydrogenosomes share a common mode of protein targeting
Dolezal, Pavel, Smíd, Ondrej, Rada, Petr, Zubácová, Zuzana, Bursać, Dejan, Suták, Robert, ...
Mitochondria are archetypal organelles of endosymbiotic origin in eukaryotic cells. Some unicellular eukaryotes (protists) were considered to be primarily amitochondrial organisms that diverged from...
The J-protein family: modulating protein assembly, disassembly and translocation
Walsh, Peter, Bursać, Dejan, Law, Yin Chern, Cyr, Douglas, Lithgow, Trevor
DnaJ is a molecular chaperone and the prototypical member of the J-protein family. J proteins are defined by the presence of a J domain that can regulate the activity of 70-kDa heat-shock proteins....
Role for yeast inhibitor of apoptosis (IAP)-like proteins in cell division
Uren, Anthony G., Beilharz, Traude, O’Connell, Matthew J., Bugg, Sarah J., Van Driel, Rosemary, Vaux, David L., ...
Inhibitors of apoptosis (IAPs) are a family of proteins that bear baculoviral IAP repeats (BIRs) and regulate apoptosis in vertebrates and Drosophila melanogaster. The yeasts Saccharomyces cerevisiae...
The yeast nascent polypeptide-associated complex initiates protein targeting to mitochondria in vivo
George, Rebecca, Beddoe, Travis, Landl, Karina, Lithgow, Trevor
The yeast nascent polypeptide-associated complex (NAC) is encoded by two genes, EGD1 and EGD2, and is associated with cytoplasmic ribosomes. Yeast mutants lacking NAC (Δegd2) are viable but suffer...
RAC, a stable ribosome-associated complex in yeast formed by the DnaK-DnaJ homologs Ssz1p and zuotin
Gautschi, Matthias, Lilie, Hauke, Fünfschilling, Ursula, Mun, Andrej, Ross, Suzanne, Lithgow, Trevor, ...
The yeast cytosol contains multiple homologs of the DnaK and DnaJ chaperone family. Our current understanding of which homologs functionally interact is incomplete. Zuotin is a DnaJ homolog bound to...
Protein structure and function by the sea
A report on the 27th Annual Lorne Conference on Protein Structure and Function, Lorne, Australia, 9-13 February 2002.
Gabriel, Kipros, Egan, Billie, Lithgow, Trevor
The Translocase of the Outer Mitochondrial membrane (TOM complex) is centred on a channel, created by Tom40, serving as the only means of entry for proteins into the mitochondrion. Proteins destined...
A SNARE required for retrograde transport to the endoplasmic reticulum
Burri, Lena, Varlamov, Oleg, Doege, Claudia A., Hofmann, Kay, Beilharz, Traude, Rothman, James E., ...
SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) are central components of the machinery mediating membrane fusion in all eukaryotic cells. Sequence analysis of the...
Chávez, Sebastián, Beilharz, Traude, Rondón, Ana G., Erdjument-Bromage, Hediye, Tempst, Paul, Svejstrup, Jesper Q., ...
Transcription-induced recombination has been reported in all organisms from bacteria to mammals. We have shown previously that the yeast genes HPR1 and THO2 may be keys to the understanding of...
Youker, Robert T., Walsh, Peter, Beilharz, Traude, Lithgow, Trevor, Brodsky, Jeffrey L.
Aberrant secreted proteins can be destroyed by ER-associated protein degradation (ERAD), and a prominent, medically relevant ERAD substrate is the cystic fibrosis transmembrane conductance regulator...
Mature DIABLO/Smac Is Produced by the IMP Protease Complex on the Mitochondrial Inner Membrane
Burri, Lena, Strahm, Yvan, Hawkins, Christine J., Gentle, Ian E., Puryer, Michelle A., Verhagen, Anne, ...
DIABLO/Smac is a mitochondrial protein that can promote apoptosis by promoting the release and activation of caspases. To do so, DIABLO/Smac must first be processed by a mitochondrial protease and...
Giardia mitosomes and trichomonad hydrogenosomes share a common mode of protein targeting
Dolezal, Pavel, Smíd, Ondrej, Rada, Petr, Zubácová, Zuzana, Bursać, Dejan, Suták, Robert, ...
Mitochondria are archetypal organelles of endosymbiotic origin in eukaryotic cells. Some unicellular eukaryotes (protists) were considered to be primarily amitochondrial organisms that diverged from...
The J-protein family: modulating protein assembly, disassembly and translocation
Walsh, Peter, Bursać, Dejan, Law, Yin Chern, Cyr, Douglas, Lithgow, Trevor
DnaJ is a molecular chaperone and the prototypical member of the J-protein family. J proteins are defined by the presence of a J domain that can regulate the activity of 70-kDa heat-shock proteins....
Microsporidian mitosomes retain elements of the general mitochondrial targeting system
Burri, Lena, Williams, Bryony A. P., Bursac, Dejan, Lithgow, Trevor, Keeling, Patrick J.
Microsporidia are intracellular parasites that infect a variety of animals, including humans. As highly specialized parasites, they are characterized by a number of unusual adaptations, many of which...
Chan, Nickie C., Lithgow, Trevor
The sorting and assembly machinery (SAM) complex functions in the assembly of β-barrel proteins into the mitochondrial outer membrane. It is related to the Omp85/YaeT machinery in bacterial outer...
The Omp85 family of proteins is essential for outer membrane biogenesis in mitochondria and bacteria
Gentle, Ian, Gabriel, Kipros, Beech, Peter, Waller, Ross, Lithgow, Trevor
Integral proteins in the outer membrane of mitochondria control all aspects of organelle biogenesis, being required for protein import, mitochondrial fission, and, in metazoans, mitochondrial aspects...
Wilson-Annan, Julie, O'Reilly, Lorraine A., Crawford, Simon A., Hausmann, George, Beaumont, Jennifer G., Parma, Loes P., ...
Prosurvival Bcl-2–like proteins, like Bcl-w, are thought to function on organelles such as the mitochondrion and to be targeted to them by their hydrophobic COOH-terminal domain. We unexpectedly...
Clements, Abigail, Gaboriaud, Fabien, Duval, Jérôme F. L., Farn, Jacinta L., Jenney, Adam W., Lithgow, Trevor, ...
Analysing the pathogenic mechanisms of a bacterium requires an understanding of the composition of the bacterial cell surface. The bacterial surface provides the first barrier against innate immune...
Protein secretion and outer membrane assembly in Alphaproteobacteria
Gatsos, Xenia, Perry, Andrew J, Anwari, Khatira, Dolezal, Pavel, Wolynec, P Peter, Likić, Vladimir A, ...
The assembly of β-barrel proteins into membranes is a fundamental process that is essential in Gram-negative bacteria, mitochondria and plastids. Our understanding of the mechanism of β-barrel...
Waller, Ross F., Jabbour, Carole, Chan, Nickie C., Celik, Nermin, Likić, Vladimir A., Mulhern, Terrence D., ...
Microsporidia are a group of highly adapted obligate intracellular parasites that are now recognized as close relatives of fungi. Their adaptation to parasitism has resulted in broad and severe...