Tsutomu Ohta

Loss of Parp-1affects gene expression profile in a genome-wide manner in ES cells and liver cells (2007)

Ogino, Hideki, Nozaki, Tadashige, Gunji, Akemi, Maeda, Miho, Suzuki, Hiroshi, Ohta, Tsutomu, ...

Abstract Background Many lines of evidence suggest that poly(ADP-ribose) polymerase-1 (Parp-1) is involved in transcriptional regulation of various genes as a coactivator or a corepressor by...

IAN Family Critically Regulates Survival and Development of T Lymphocytes (2006)

Takeshi Nitta, Mariam Nasreen, Takafumi Seike, Atsushi Goji, Izumi Ohigashi, Tadaaki Miyazaki, ...

The authors describe new roles for IAN proteins (a previously uncharacterized family of GTP binding proteins) in T cell development; different IAN proteins play important roles at distinct stages of...

IAN Family Critically Regulates Survival and Development of T Lymphocytes (2006)

Takeshi Nitta, Mariam Nasreen, Takafumi Seike, Atsushi Goji, Izumi Ohigashi, Tadaaki Miyazaki, ...

The IAN (immune-associated nucleotide-binding protein) family is a family of functionally uncharacterized GTP-binding proteins expressed in vertebrate immune cells and in plant cells during...

Association of polymorphisms in the MTH1 gene with small cell lung carcinoma risk (2006)

Kohno, Takashi, Sakiyama, Tokuki, Kunitoh, Hideo, Goto, Koichi, Nishiwaki, Yutaka, Saito, Daizo, ...

Fifty single-nucleotide polymorphisms (SNPs) associated with amino acid changes in 36 genes involved in diverse DNA repair pathways were assessed for associations with risk for small cell lung...

Association of polymorphisms in the MTH1 gene with small cell lung carcinoma risk (2006)

Kohno, Takashi, Sakiyama, Tokuki, Kunitoh, Hideo, Goto, Koichi, Nishiwaki, Yutaka, Saito, Daizo, ...

Fifty single-nucleotide polymorphisms (SNPs) associated with amino acid changes in 36 genes involved in diverse DNA repair pathways were assessed for associations with risk for small cell lung...

Histone methyltransferases G9a and GLP form heteromeric complexes and are both crucial for methylation of euchromatin at H3-K9 (2005)

Tachibana, Makoto, Ueda, Jun, Fukuda, Mikiko, Takeda, Naoki, Ohta, Tsutomu, Iwanari, Hiroko, ...

Histone H3 Lys 9 (H3-K9) methylation is a crucial epigenetic mark for transcriptional silencing. G9a is the major mammalian H3-K9 methyltransferase that targets euchromatic regions and is essential...

Histone methyltransferases G9a and GLP form heteromeric complexes and are both crucial for methylation of euchromatin at H3-K9 (2005)

Tachibana, Makoto, Ueda, Jun, Fukuda, Mikiko, Takeda, Naoki, Ohta, Tsutomu, Iwanari, Hiroko, ...

Histone H3 Lys 9 (H3-K9) methylation is a crucial epigenetic mark for transcriptional silencing. G9a is the major mammalian H3-K9 methyltransferase that targets euchromatic regions and is essential...

Histone methyltransferases G9a and GLP form heteromeric complexes and are both crucial for methylation of euchromatin at H3-K9 (2005)

Tachibana, Makoto, Ueda, Jun, Fukuda, Mikiko, Takeda, Naoki, Ohta, Tsutomu, Iwanari, Hiroko, ...

Histone H3 Lys 9 (H3-K9) methylation is a crucial epigenetic mark for transcriptional silencing. G9a is the major mammalian H3-K9 methyltransferase that targets euchromatic regions and is essential...

Nucleosomal histone kinase-1 phosphorylates H2A Thr 119 during mitosis in the early Drosophila embryo (2004)

Aihara, Hitoshi, Nakagawa, Takeya, Yasui, Kiyoshi, Ohta, Tsutomu, Hirose, Susumu, Dhomae, Naoshi, ...

Posttranslational histone modifications are important for the regulation of many biological phenomena. Here, we show the purification and characterization of nucleosomal histone kinase-1 (NHK-1)....

Global gene expression analysis of rat colon cancers induced by a food-borne carcinogen, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (2004)

Fujiwara, Kyoko, Ochiai, Masako, Ohta, Tsutomu, Ohki, Misao, Aburatani, Hiroyuki, Nagao, Minako, ...

Colon cancers develop after accumulation of multiple genetic and epigenetic alterations in colon epithelial cells. To shed light on global changes in gene expression of colon cancers, and to gain...

Global gene expression analysis of rat colon cancers induced by a food-borne carcinogen, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (2004)

Fujiwara, Kyoko, Ochiai, Masako, Ohta, Tsutomu, Ohki, Misao, Aburatani, Hiroyuki, Nagao, Minako, ...

Colon cancers develop after accumulation of multiple genetic and epigenetic alterations in colon epithelial cells. To shed light on global changes in gene expression of colon cancers and to gain...

Nucleosomal histone kinase-1 phosphorylates H2A Thr 119 during mitosis in the early Drosophila embryo (2004)

Aihara, Hitoshi, Nakagawa, Takeya, Yasui, Kiyoshi, Ohta, Tsutomu, Hirose, Susumu, Dhomae, Naoshi, ...

Posttranslational histone modifications are important for the regulation of many biological phenomena. Here, we show the purification and characterization of nucleosomal histone kinase-1 (NHK-1)....

Nucleosomal histone kinase-1 phosphorylates H2A Thr 119 during mitosis in the early Drosophila embryo (2004)

Aihara, Hitoshi, Nakagawa, Takeya, Yasui, Kiyoshi, Ohta, Tsutomu, Hirose, Susumu, Dhomae, Naoshi, ...

Posttranslational histone modifications are important for the regulation of many biological phenomena. Here, we show the purification and characterization of nucleosomal histone kinase-1 (NHK-1)....

Global expression analysis of N-methyl-N'-nitro-N-nitrosoguanidine-induced rat stomach carcinomas using oligonucleotide microarrays (2003)

Abe, Masanobu, Yamashita, Satoshi, Kuramoto, Takashi, Hirayama, Yoshikazu, Tsukamoto, Tetsuya, Ohta, Tsutomu, ...

Rat stomach carcinomas induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) are widely used as a model for differentiated-type human stomach carcinomas. Here, we analyzed expression profiles in...

Global expression analysis of N-methyl-N'-nitro-N-nitrosoguanidine-induced rat stomach carcinomas using oligonucleotide microarrays (2003)

Abe, Masanobu, Yamashita, Satoshi, Kuramoto, Takashi, Hirayama, Yoshikazu, Tsukamoto, Tetsuya, Ohta, Tsutomu, ...

Rat stomach carcinomas induced by N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) are widely used as a model for differentiated-type human stomach carcinomas. Here, we analyzed expression profiles in...

Global expression analysis of N-methyl-N'-nitro-N-nitrosoguanidine-induced rat stomach carcinomas using oligonucleotide microarrays (2003)

Abe, Masanobu, Yamashita, Satoshi, Kuramoto, Takashi, Hirayama, Yoshikazu, Tsukamoto, Tetsuya, Ohta, Tsutomu, ...

Rat stomach carcinomas induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) are widely used as a model for differentiated-type human stomach carcinomas. Here, we analyzed expression profiles in...

Structural Composition of Hammerhead Ribozymes (1995)

Takenaka, Akio, Matsumoto, Osamu, Chen, Yixin, Hasegawa, Sei-ichi, Chatake, Toshiyuki, Tsunoda, Masaru, ...

Eleven kinds of hammerhead ribozymes were designed and synthesized to investigate the structural composition of the complexes and to find suitable crystallization conditions, the substrate chains...

Negative Supercoiling of DNA by Eukaryotic DNA Topoisomerase II and Dextran Sulfate (1991)

Okada, Kohichi, Ohta, Tsutomu, Hirose, Susumu

In the presence of a molar excess of eukaryotic DNA topoisomerase II and an appropriate concentration of dextran sulfate, relaxed closed circular DNA is converted to a negatively supercoiled form....

DNA Supercoiling Factor Localizes to Puffs on Polytene Chromosomes in Drosophila melanogaster

Kobayashi, Masatomo, Aita, Noriko, Hayashi, Shigeo, Okada, Kohichi, Ohta, Tsutomu, Hirose, Susumu

DNA supercoiling factor (SCF) was first identified in silkworm as a protein that generates negative supercoils in DNA in conjunction with eukaryotic topoisomerase II. To analyze the in vivo role of...

G9a histone methyltransferase plays a dominant role in euchromatic histone H3 lysine 9 methylation and is essential for early embryogenesis

Tachibana, Makoto, Sugimoto, Kenji, Nozaki, Masami, Ueda, Jun, Ohta, Tsutomu, Ohki, Misao, ...

Covalent modification of histone tails is crucial for transcriptional regulation, mitotic chromosomal condensation, and heterochromatin formation. Histone H3 lysine 9 (H3-K9) methylation catalyzed by...

Nucleosomal histone kinase-1 phosphorylates H2A Thr 119 during mitosis in the early Drosophila embryo

Aihara, Hitoshi, Nakagawa, Takeya, Yasui, Kiyoshi, Ohta, Tsutomu, Hirose, Susumu, Dhomae, Naoshi, ...

Posttranslational histone modifications are important for the regulation of many biological phenomena. Here, we show the purification and characterization of nucleosomal histone kinase-1 (NHK-1)....

Histone methyltransferases G9a and GLP form heteromeric complexes and are both crucial for methylation of euchromatin at H3-K9

Tachibana, Makoto, Ueda, Jun, Fukuda, Mikiko, Takeda, Naoki, Ohta, Tsutomu, Iwanari, Hiroko, ...

Histone H3 Lys 9 (H3-K9) methylation is a crucial epigenetic mark for transcriptional silencing. G9a is the major mammalian H3-K9 methyltransferase that targets euchromatic regions and is essential...

A novel high-throughput (HTP) cloning strategy for site-directed designed chimeragenesis and mutation using the Gateway cloning system

Suzuki, Yasuhiro, Kagawa, Naoko, Fujino, Toru, Sumiya, Tsuyoshi, Andoh, Taichi, Ishikawa, Kumiko, ...

There is an increasing demand for easy, high-throughput (HTP) methods for protein engineering to support advances in the development of structural biology, bioinformatics and drug design. Here, we...

IAN Family Critically Regulates Survival and Development of T Lymphocytes

Nitta, Takeshi, Nasreen, Mariam, Seike, Takafumi, Goji, Atsushi, Ohigashi, Izumi, Miyazaki, Tadaaki, ...

The IAN (immune-associated nucleotide-binding protein) family is a family of functionally uncharacterized GTP-binding proteins expressed in vertebrate immune cells and in plant cells during...

Disruption of Spermatogenic Cell Adhesion and Male Infertility in Mice Lacking TSLC1/IGSF4, an Immunoglobulin Superfamily Cell Adhesion Molecule†

Yamada, Daisuke, Yoshida, Midori, Williams, Yuko N., Fukami, Takeshi, Kikuchi, Shinji, Masuda, Mari, ...

TSLC1/IGSF4, an immunoglobulin superfamily molecule, is predominantly expressed in the brain, lungs, and testes and plays important roles in epithelial cell adhesion, cancer invasion, and synapse...

DNA Supercoiling Factor Localizes to Puffs on Polytene Chromosomes in Drosophila melanogaster

Kobayashi, Masatomo, Aita, Noriko, Hayashi, Shigeo, Okada, Kohichi, Ohta, Tsutomu, Hirose, Susumu

DNA supercoiling factor (SCF) was first identified in silkworm as a protein that generates negative supercoils in DNA in conjunction with eukaryotic topoisomerase II. To analyze the in vivo role of...

G9a histone methyltransferase plays a dominant role in euchromatic histone H3 lysine 9 methylation and is essential for early embryogenesis

Tachibana, Makoto, Sugimoto, Kenji, Nozaki, Masami, Ueda, Jun, Ohta, Tsutomu, Ohki, Misao, ...

Covalent modification of histone tails is crucial for transcriptional regulation, mitotic chromosomal condensation, and heterochromatin formation. Histone H3 lysine 9 (H3-K9) methylation catalyzed by...

Nucleosomal histone kinase-1 phosphorylates H2A Thr 119 during mitosis in the early Drosophila embryo

Aihara, Hitoshi, Nakagawa, Takeya, Yasui, Kiyoshi, Ohta, Tsutomu, Hirose, Susumu, Dhomae, Naoshi, ...

Posttranslational histone modifications are important for the regulation of many biological phenomena. Here, we show the purification and characterization of nucleosomal histone kinase-1 (NHK-1)....

Histone methyltransferases G9a and GLP form heteromeric complexes and are both crucial for methylation of euchromatin at H3-K9

Tachibana, Makoto, Ueda, Jun, Fukuda, Mikiko, Takeda, Naoki, Ohta, Tsutomu, Iwanari, Hiroko, ...

Histone H3 Lys 9 (H3-K9) methylation is a crucial epigenetic mark for transcriptional silencing. G9a is the major mammalian H3-K9 methyltransferase that targets euchromatic regions and is essential...

A novel high-throughput (HTP) cloning strategy for site-directed designed chimeragenesis and mutation using the Gateway cloning system

Suzuki, Yasuhiro, Kagawa, Naoko, Fujino, Toru, Sumiya, Tsuyoshi, Andoh, Taichi, Ishikawa, Kumiko, ...

There is an increasing demand for easy, high-throughput (HTP) methods for protein engineering to support advances in the development of structural biology, bioinformatics and drug design. Here, we...

IAN Family Critically Regulates Survival and Development of T Lymphocytes

Nitta, Takeshi, Nasreen, Mariam, Seike, Takafumi, Goji, Atsushi, Ohigashi, Izumi, Miyazaki, Tadaaki, ...

The IAN (immune-associated nucleotide-binding protein) family is a family of functionally uncharacterized GTP-binding proteins expressed in vertebrate immune cells and in plant cells during...

Disruption of Spermatogenic Cell Adhesion and Male Infertility in Mice Lacking TSLC1/IGSF4, an Immunoglobulin Superfamily Cell Adhesion Molecule†

Yamada, Daisuke, Yoshida, Midori, Williams, Yuko N., Fukami, Takeshi, Kikuchi, Shinji, Masuda, Mari, ...

TSLC1/IGSF4, an immunoglobulin superfamily molecule, is predominantly expressed in the brain, lungs, and testes and plays important roles in epithelial cell adhesion, cancer invasion, and synapse...

BRG1 Interacts with Nrf2 To Selectively Mediate HO-1 Induction in Response to Oxidative Stress▿

Zhang, Jianyong, Ohta, Tsutomu, Maruyama, Atsushi, Hosoya, Tomonori, Nishikawa, Keizo, Maher, Jonathan M., ...

NF-E2-related factor 2 (Nrf2) regulates antioxidant-responsive element-mediated induction of cytoprotective genes in response to oxidative stress. The purpose of this study was to determine the role...

The SYT-SSX Fusion Protein Down-Regulates the Cell Proliferation Regulator COM1 in t(x;18) Synovial Sarcoma▿ †

Ishida, Michiko, Miyamoto, Mamiko, Naitoh, Sayu, Tatsuda, Daisuke, Hasegawa, Tadashi, Nemoto, Takeshi, ...

Chromosomal translocations are frequently associated with soft-tissue sarcomas. Fusion proteins generated by such translocations often play critical roles in tumorigenesis. Therefore, it is important...

Expression Profiling in Ovarian Clear Cell Carcinoma : Identification of Hepatocyte Nuclear Factor-1β as a Molecular Marker and a Possible Molecular Target for Therapy of Ovarian Clear Cell Carcinoma

Tsuchiya, Akira, Sakamoto, Michiie, Yasuda, Jun, Chuma, Makoto, Ohta, Tsutomu, Ohki, Misao, ...

Of all of the epithelial ovarian cancers, clear cell carcinoma (CCC) of the ovary has the worst prognosis. We applied the oligonucleotide array technique to identify genes generally involved in CCC....

Nrf2 Neh5 domain is differentially utilized in the transactivation of cytoprotective genes

Zhang, Jianyong, Hosoya, Tomonori, Maruyama, Atsushi, Nishikawa, Keizo, Maher, Jonathan M., Ohta, Tsutomu, ...

The transcription factor Nrf2 (nuclear factor erythroid 2-related factor 2) contains two transcription activation domains, Neh4 (Nrf2 ECH homology 4) and Neh5, which co-ordinately regulate...

Cancer related mutations in NRF2 impair its recognition by Keap1-Cul3 E3 ligase and promote malignancy

Shibata, Tatsuhiro, Ohta, Tsutomu, Tong, Kit I., Kokubu, Akiko, Odogawa, Reiko, Tsuta, Koji, ...

The nuclear factor E2-related factor 2 (Nrf2) is a master transcriptional activator of genes encoding numerous cytoprotective enzymes that are induced in response to environmental and endogenously...