Tsutomu Shimada

Obesity-induced increase of CYP2E1 activity and its effect on disposition kinetics of chlorzoxazone in Zucker rats (2007)

Khemawoot, Phisit, Yokogawa, Koichi, Shimada, Tsutomu, Miyamoto, Ken-ichi

This study was designed to investigate the induction of CYP2E1 in obese Zucker rats and its effect on the disposition kinetics of chlorzoxazone (CZX). CZX 20 mg/kg was administered to three groups of...

Site-dependent contributions of P-glycoprotein and CYP3A to cyclosporin A absorption, and effect of dexamethasone in small intestine of mice (2006)

Jin, Ingji, Shimada, Tsutomu, Yokogawa, Koichi, Nomura, Masaaki, Ishizaki, Junko, Piao, Yingshi, ...

We examined whether the oral bioavailability of cyclosporin A is controlled primarily by P-glycoprotein (P-gp) or CYP3A in the small intestine. In situ loop method was used to evaluate the uptake of...

Activation of aminophenylnorharman, aminomethylphenylnorharman and aminophenylharman to genotoxic metabolites by human N-acetyltransferases and cytochrome P450 enzymes expressed in Salmonella typhimurium umu tester strains (2006)

Oda, Yoshimitsu, Totsuka, Yukari, Wakabayashi, Keiji, Guengerich, F.Peter, Shimada, Tsutomu

Norharman (9H-pyrido[3,4-b]indole) and harman (1-methyl-9H-pyrido[3,4-b]indole) contained in cigarette smoke and cooked foodstuffs, are non-mutagenic to Salmonella strains, but show co-mutagenicity...

Activation of aminophenylnorharman, aminomethylphenylnorharman and aminophenylharman to genotoxic metabolites by human N-acetyltransferases and cytochrome P450 enzymes expressed in Salmonella typhimurium umu tester strains (2006)

Oda, Yoshimitsu, Totsuka, Yukari, Wakabayashi, Keiji, Guengerich, F. Peter, Shimada, Tsutomu

Norharman (9H-pyrido[3,4-b]indole) and harman (1-methyl-9H-pyrido[3,4-b]indole) contained in cigarette smoke and cooked foodstuffs, are non-mutagenic to Salmonella strains, but show co-mutagenicity...

Laser-ablation treatment of short-pulse laser targets: Toward an experimental program on energetic-ion interactions (2005)

Fernández,Juan C., Hegelich,B. Manuel, Cobble,James A., Flippo,Kirk A., Letzring,Samuel A., Johnson,Randall P., ...

Laser-ablation treatment of short-pulse laser targets: Toward an experimental program on energetic-ion interactions (2005)

Fernández, Juan C., Hegelich, B. Manuel, Cobble, James A., Flippo, Kirk A., Letzring, Samuel A., Johnson, Randall P., ...

Arylhydrocarbon receptor-dependent induction of liver and lung cytochromes P450 1A1, 1A2, and 1B1 by polycyclic aromatic hydrocarbons and polychlorinated biphenyls in genetically engineered C57BL/6J mice (2002)

Shimada, Tsutomu, Inoue, Kiyoshi, Suzuki, Yoshihiko, Kawai, Takao, Azuma, Emiko, Nakajima, Takae, ...

Arylhydrocarbon receptor knock-out, AhR(–/–), mice have recently been shown to be rather resistant to benzo[a]pyrene (B[a]P)-induced tumor formation, probably reflecting the...

Roles of NADPH-P450 reductase in the O-Deethylation of 7-Ethoxycoumarin by recombinant human cytochrome P450 1B1 variants in Escherichia coli (2000)

Shimada, Tsutomu, Tsumura, Fujiko, Gillam, Elizabeth M. J., Guengerich, F. Peter, Inoue, Kiyoshi

Roles of NADPH-P450 reductase in the O-Deethylation of 7-Ethoxycoumarin by recombinant human cytochrome P450 1B1 variants in Escherichia coli (2000)

Shimada, Tsutomu, Tsumura, Fujiko, Gillam, Elizabeth M. J., Guengerich, F. Peter, Inoue, Kiyoshi

Catalytic properties of polymorphic human cytochrome P450 1B1 variants (1999)

Shimada, Tsutomu, Watanabe, Junko, Kawajiri, Kaname, Sutter, Thomas R., Guengerich, F. Peter, Gillam, Elizabeth M. J., ...

Metabolism of Benzo(a)pyrene to trans-7,8Dihydroxy-7,8-dihydrobenzo(a)pyrene by Recombinant Human Cytochrome P450 1B1 and Purified Liver Epoxide Hydrolase (1999)

Shimada, Tsutomu, Gillam, Elizabeth M. J., Oda, Yoshimitsu, Tsumura, Fujiko, Sutter, Thomas R., Guengerich, F. Peter, ...

Catalytic properties of polymorphic human cytochrome P450 1B1 variants (1999)

Shimada, Tsutomu, Watanabe, Junko, Kawajiri, Kaname, Sutter, Thomas R., Guengerich, F. Peter, Gillam, Elizabeth M. J., ...

Metabolism of Benzo(a)pyrene to trans-7,8Dihydroxy-7,8-dihydrobenzo(a)pyrene by Recombinant Human Cytochrome P450 1B1 and Purified Liver Epoxide Hydrolase (1999)

Shimada, Tsutomu, Gillam, Elizabeth M. J., Oda, Yoshimitsu, Tsumura, Fujiko, Sutter, Thomas R., Guengerich, F. Peter, ...

Role of human N-acetyltransferases, NAT1 or NAT2, in genotoxicity of nitroarenes and aromatic amines in Salmonella typhimurium NM6001 and NM6002 (1999)

Oda, Yoshimitsu, Yamazaki, Hiroshi, Shimada, Tsutomu

Human NAT1 and NAT2 genes were subcloned into pACYC184 vector and the plasmids thus obtained were introduced into Salmonella typhimurium O-acetyltransferase-deficient strain NM6000...

Catalytic properties of polymorphic human cytochrome P450 1B1 variants (1999)

Shimada, Tsutomu, Watanabe, Junko, Kawajiri, Kaname, Sutter, Thomas R., Guengerich, F. Peter, Gillam, Elizabeth M.J., ...

Four polymorphic human cytochrome P450 (CYP) 1B1 allelic variants, namely Arg48,Ala119,Leu432,Asn453, Arg48,Ser119,Leu432,Asn453, Arg48,Ala119,Val432,Asn-453 and Arg48,Ser119,Val432,Asn453, were...

A new Salmonella typhimurium NM5004 strain expressing rat glutathione S-transferase 5-5: use in detection of genotoxicity of dihaloalkanes using an SOS/umu test system (1996)

Oda, Yoshimitsu, Yamazaki, Hiroshi, Thier, Ricarda, Ketterer, Brian, Guengerich, F.Peter, Shimada, Tsutomu

The Escherichia coli mu operon was subcloned into a pKK233–2 vector containing rat glutathione S-transferase (GST) 5–5 cDNA and the plasmid thus obtained was introduced into Salmonella...

Procarcinogen activation by cytochrome P450 3A4 and 3A5 expressed in Escherichia coli and by human liver microsomes (1995)

Yamazaki, Hiroshi, Inui, Yukiharu, Wrighton, Steven A., Guengerich, F.Peter, Shimada, Tsutomu

Recent studies indicate that cytochrome P450 (P450) 3A4 plays important roles in the activation of procarcinogens such as aflatoxin B1 and sterigmatocystin, as well as in the oxidation of a number of...

Activation of trans-l,2-dihydro-l,2-dihydroxy-6-aminochrysene to genotoxic metabolites by rat and human cytochromes P450 (1994)

Yamazaki, Hiroshi, Mimura, Mayumi, Oda, Yoshimitsu, Gonzalez, Frank J., El-Bayoumy, Karam, Chae, Young-Heum, ...

In order to address the hypothesis that 6-aminochrysene (6-AC) is converted to genotoxic products by cytochrome P450 enzymes via two activation pathways (N-hydroxylation and epoxidation), the...

Activation of procarcinogens by human cytochrome P450 enzymes expressed in Escherichia coli. Simplified bacterial systems for genotoxicity assays (1994)

Shimada, Tsutomu, Gillam, Elizabeth M.J., Sandhu, Punam, Guo, Zuyu, Tukey, Robert H., Guengerich, F.Peter

Bacterial assays were used to examine the activation of 14 known procarcinogens by cytochrome P450 (P450) enzymest†. Human P450s 1A1, 1A2 and 3A4 were expressed in Escherichia coil with slight...

Participation of rat liver cytochrome P450 2E1 in the activation of N-nitrosodimethylamine and N-nitrosodiethylainine to products genotoxic in an acetyltransferase-overexpressing Salmonella typhimurium strain (NM2009) (1992)

Yamazaki, Hiroshi, Oda, Yoshimitsu, Funae, Yoshihiko, Imaoka, Susumu, Inui, Yukiharu, Guengerich, F.Peter, ...

The possible roles of cytochrome P450 (P450) enzymes in the metabolic activation of N-nitrosodimethylamine (NDMA) and N-nitrosodiethylamine (NDEA) by rat liver microsomes have been examined in a...

Cytochrome P450 2E1 and 2A6 enzymes as major catalysts for metabolic activation of N-nitrosodialkylamines and tobacco-related nitrosamines in human liver microsomes (1992)

Yamazaki, Hiroshi, Inui, Yukiharu, Yun, Chul-Ho, Guengerich, F.Peter, Shimada, Tsutomu

An acetyltransferase-overexpressing strain of Salmonella typhimurium (NM2009) has been used to investigate roles of human liver microsomal cytochrome P450 (P450) enzymes in the activation of...

Roles of different cytochrome P450 enzymes in bioactivation of the potent hepatocarcinogen 3-methoxy-4-aminoazobenzene by rat and human liver microsomes (1991)

Yamazaki, Hiroshi, Degawa, Masakuni, Funae, Yoshihiko, Imaoka, Susumu, Inui, Yukiharu, Guengerich, F.Peter, ...

The potent hepatocarcinogen 3-methoxy-4-aminoazobenzene (3-MeO-AAB) has been reported to be bioactivated to mutagenic intermediates by rat liver microsomal cytochrome P450 (P450) and to be a...

Metabolic deactivation of furylfuramide by cytothrome P450 in human and liver microsomes (1990)

Shimada, Tsutomu, Yamazaki, Hiroshi, Shimura, Hisashi, Tanaka, Ryo-ichi, Guengerich, F.Peter

Metabolic deactivation of furylfuramide by human and rat liver microsomal cytochrome P450 enzymes has been investigated in a system measuring induction of umu gene expression response in Salmonella...

Site-dependent contributions of P-glycoprotein and CYP3A to cyclosporin A absorption, and effect of dexamethasone in small intestine of mice

Jin, Ingji, Shimada, Tsutomu, Yokogawa, Koichi, Nomura, Masaaki, Ishizaki, Junko, Piao, Yingshi, ...

We examined whether the oral bioavailability of cyclosporin A is controlled primarily by P-glycoprotein (P-gp) or CYP3A in the small intestine. In situ loop method was used to evaluate the uptake of...

Site-dependent contributions of P-glycoprotein and CYP3A to cyclosporin A absorption, and effect of dexamethasone in small intestine of mice

Jin, Ingji, Shimada, Tsutomu, Yokogawa, Koichi, Nomura, Masaaki, Ishizaki, Junko, Piao, Yingshi, ...

We examined whether the oral bioavailability of cyclosporin A is controlled primarily by P-glycoprotein (P-gp) or CYP3A in the small intestine. In situ loop method was used to evaluate the uptake of...

Obesity-induced increase of CYP2E1 activity and its effect on disposition kinetics of chlorzoxazone in Zucker rats

Khemawoot, Phisit, Yokogawa, Koichi, Shimada, Tsutomu, Miyamoto, Ken-ichi

This study was designed to investigate the induction of CYP2E1 in obese Zucker rats and its

Effects of Green Tea Fractions on Oxygen-Induced Retinal Neovascularization in the Neonatal Rat

Saito, Yuta, Hasebe-Takenaka, Yasuko, Ueda, Toshihiko, Nakanishi-Ueda, Takako, Kosuge, Shotaro, Aburada, Masaki, ...

This study aimed to investigate the preventive effects of green tea fractions (GTFs) on rat model of oxygen-induced retinopathy (OIR). Neonatal Sprague-Dawley rats were exposed to daily cycles of 80%...