Scientific Commons: Tuneko Okazaki

Human centromere protein A (CENP-A) can replace histone H3 in nucleosome reconstitution in vitro

Yoda, Kinya, Ando, Satoshi, Morishita, Setsuo, Houmura, Kenichi, Hashimoto, Keiji, Takeyasu, Kunio, ...

Centromere protein A (CENP-A) is a variant of histone H3 with more than 60% sequence identity at the C-terminal histone fold domain. CENP-A specifically locates to active centromeres of animal...

CENP-A, -B, and -C Chromatin Complex That Contains the I-Type α-Satellite Array Constitutes the Prekinetochore in HeLa Cells

Ando, Satoshi, Yang, Hua, Nozaki, Naohito, Okazaki, Tuneko, Yoda, Kinya

CENP-A is a component of centromeric chromatin and defines active centromere regions by forming centromere-specific nucleosomes. We have isolated centromeric chromatin containing the CENP-A...

MECHANISM OF DNA CHAIN GROWTH, IV. DIRECTION OF SYNTHESIS OF T4 SHORT DNA CHAINS AS REVEALED BY EXONUCLEOLYTIC DEGRADATION*

Okazaki, Tuneko, Okazaki, Reiji

T4 nascent short chains labeled at their growing ends with H3-thymidine and uniformly with C14-thymidine were prepared, separated into complementary strands, and degraded by E. coli exonuclease I in...

MECHANISM OF DNA CHAIN GROWTH, III. EQUAL ANNEALING OF T4 NASCENT SHORT DNA CHAINS WITH THE SEPARATED COMPLEMENTARY STRANDS OF THE PHAGE DNA*

Sugimoto, Kazunori, Okazaki, Tuneko, Imae, Yasuo, Okazaki, Reiji

Nascent short DNA chains isolated from T4-infected E. coli under a variety of conditions anneal equally to the separated complementary phage DNA strands. The samples examined include: pulse-labeled...

THYMIDINE DIPHOSPHATE-l-RHAMNOSE METABOLISM IN SMOOTH AND ROUGH STRAINS OF ESCHERICHIA COLI AND SALMONELLA WESLACO

Okazaki, Tuneko, Strominger, Jack L., Okazaki, Reiji

Okazaki, Tuneko (Washington University School of Medicine, St. Louis, Mo.), Jack L. Stominger, and Reiji Okazaki. Thymidine diphosphate-l-rhamnose metabolism in smooth and rough strains of...

RNA-linked nascent DNA pieces in phage T7-infected Escherichia coli. III. Detection of intact primer RNA

Ogawa, Tohru, Okazaki, Tuneko

RNA-linked DNA fragments of T7-infected Escherichiacoli were labeled with [32P]orthophosphate invivo. The RNA segments of the labeled fragments were isolated by degrading the DNA portion with the...