Takahashi, Junko, Rudsander, Ulla J., Hedenström, Mattias, Banasiak, Alicja, Harholt, Jesper, Amelot, Nicolas, ...
KORRIGAN1 (KOR1) is a membrane-bound cellulase implicated in cellulose biosynthesis. PttCel9A1 from hybrid aspen (Populus tremula L. × tremuloides Michx.) has high sequence similarity to KOR1...
tremula × tremuloides) xyloglucan endotransglycosylase 16A expressed (2008)
In Pichia Pastoris, Kathleen Piens, Stuart E. Denman, Hongbin Henriksson, Jenny Fäldt, ...
Enzymatic properties of native and deglycosylated hybrid aspen (Populus
Nishikubo, Nobuyuki, Awano, Tatsuya, Banasiak, Alicja, Bourquin, Veronica, Ibatullin, Farid, Funada, Ryo, ...
Tension wood is a specialized tissue of deciduous trees that functions in bending woody stems to optimize their position in space. Tension wood fibers that develop on one side of the stem have an...
Ubeda-Tomas, Susana, Edvardsson, Ellinor, Eland, Cathlene, Singh, Kumar, Zadik, Daniel, Aspeborg, Henrik, ...
Despite the importance of secondary growth in plants, relatively few genes regulating this process have been identified to date. By using data from detailed transcript profiling of the poplar...
Patrik Johansson, B Hongbin Henriksson, Stuart E. Denman, Tuula T. Teeri, ...
have been edited and the authors have corrected proofs, but before the final, complete issue is published online. Early posting of articles reduces normal time to publication by several weeks.
Functional genomics of wood formation in hybrid aspen (2003)
Aspeborg, Henrik, Blomqvist, Kristina, Denman, Stuart, Hertzberg, Magnus, Ohlsson, Anna, Berglund, Torkel, ...
Stuart Denman, Harry Brumer, Asa M. Kallas, Åsa M. Kallas, Hongbin Henriksson, Terese Bergfors, ...
this paper may load this reprint on their own web site provided that this cover page is retained. Republication of this article or its storage in electronic databases or the like is not permitted...
Poplar Cell Suspension Cultures as a Tool to Study Wood Formation (2002)
Ohlsson, Anna B., Blomqvist, Kristina, Aspeborg, Henrik, Li, Xingou, Andersson, Anders, Henriksson, Hongbin, ...
Gustavsson, Malin, Lehtiö, Janne, Denman, Stuart, Teeri, Tuula T., Hult, Karl, Martinelle, Mats
Fusion proteins composed of a cellulose-binding domain from Neocallimastix patriciarum cellulase A and Candida antarctica lipase B were constructed using different linker peptides. The aim was to...
Sterky, Fredrik, Regan, Sharon, Karlsson, Jan, Herzberg, Magnus, Rohde, Antje, Holmberg, Anders, ...
The active site of Trichoderma reesei cellobiohydrolase II: the role of tyrosine 169 (1996)
Koivula, Anu, Reinikainen, Tapani, Ruohonen, Laura, Valkeajärvi, Anne, Claeyssens, Marc, Teleman, Olle, ...
Trichoderma reesei cellobiohydrolase II (CBHII) is an exoglucanase cleaving primarily cellobiose units from the non-reducing end of cellulose chains. The β-l,4 glycosidic bond is cleaved by acid...
Properties of a single-chain antibody containing different linker peptides (1995)
Alfthan, Kaija, Takkinen, Kristiina, Sizmann, Dorothea, Söderlund, Hans, Teeri, Tuula T.
Single-chain antibodies were constructed using six different linker peptides to join the VH and VL domains of an anti-2-phenyloxazolone (Ox) antibody. Four of the linker peptides originated from the...
Hemminki, Ari, Hoffrén, Anna-Marja, Takkinen, Kristiina, Vehniäinen, Markus, Mäkinen, Maija-Liisa, Pettersson, Kim, ...
Europium chelates provide a non-radioactive alternative for sensitive labelling of antibodies for diagnostic immunoassays. Lysine residues at antibody surfaces are ready targets for labelling by an...
Hoffrén, Anna-Marja, Teeri, Tuula T., Teleman, Olle
A total of 23 fungal cellulose-binding domain (CBD) sequences were aligned. Structural models of the cellulosebinding domain of an exoglucanase (CBHII) and of three endoglucanases (EGI, EGII and EGV)...
Laukkanen, Marja-Leena, Teeri, Tuula T., Keinänen, Kari
In order to achieve a stable and functional immobilization of antibodies, we investigated the possibility of adding hydrophobic membrane anchors to antibody fragments expressed in Escherichia coli....
Takkinen, Kristiina, Laukkanen, Marja-Leena, Sizmann, Dorothea, Alfthan, Kaija, Immonen, Tiina, Vanne, Liisa, ...
Single-chain antibodies consist of the variable, antigen-binding domains of antibodies joined to a continuous polypeptide by genetically engineered peptide linkers. We have used the flexible...
Holm, Liisa, Laaksonen, Leif, Kaartinen, Matti, Teeri, Tuula T., Knowles, Jonathan K.C.
Structural models of the variable domains of the murine anti-2-phenyloxazolone IgG (Oxl idiotype) and its somatic variant, which has higher affinity to the hapten 2-phenyloxazolone, were constructed...
Tiivistelmä ja 4 erip.
Gene discovery in the wood-forming tissues of poplar: Analysis of 5,692 expressed sequence tags
Sterky, Fredrik, Regan, Sharon, Karlsson, Jan, Hertzberg, Magnus, Rohde, Antje, Holmberg, Anders, ...
A rapidly growing area of genome research is the generation of expressed sequence tags (ESTs) in which large numbers of randomly selected cDNA clones are partially sequenced. The collection of ESTs...
A transcriptional roadmap to wood formation
Hertzberg, Magnus, Aspeborg, Henrik, Schrader, Jarmo, Andersson, Anders, Erlandsson, Rikard, Blomqvist, Kristina, ...
The large vascular meristem of poplar trees with its highly organized secondary xylem enables the boundaries between different developmental zones to be easily distinguished. This property of...
Vanden Wymelenberg, Amber, Denman, Stuart, Dietrich, Diane, Bassett, Jennifer, Yu, Xiaochun, Atalla, Rajai, ...
Phanerochaete chrysosporium cellulase genes were cloned and characterized. The cel61A product was structurally similar to fungal endoglucanases of glycoside hydrolase family 61, whereas the cel9A...
The binding specificity and affinity determinants of family 1 and family 3 cellulose binding modules
Lehtiö, Janne, Sugiyama, Junji, Gustavsson, Malin, Fransson, Linda, Linder, Markus, Teeri, Tuula T.
Cellulose binding modules (CBMs) potentiate the action of cellulolytic enzymes on insoluble substrates. Numerous studies have established that three aromatic residues on a CBM surface are needed for...
Johansson, Patrik, Brumer, Harry, Baumann, Martin J., Kallas, Åsa M., Henriksson, Hongbin, Denman, Stuart E., ...
Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Thus, XET is a key enzyme in all plant processes that require...
Expansins Abundant in Secondary Xylem Belong to Subgroup A of the α-Expansin Gene Family1[w]
Gray-Mitsumune, Madoka, Mellerowicz, Ewa J., Abe, Hisashi, Schrader, Jarmo, Winzéll, Anders, Sterky, Fredrik, ...
Differentiation of xylem cells in dicotyledonous plants involves expansion of the radial primary cell walls and intrusive tip growth of cambial derivative cells prior to the deposition of a thick...
Carbohydrate-Active Enzymes Involved in the Secondary Cell Wall Biogenesis in Hybrid Aspen1
Aspeborg, Henrik, Schrader, Jarmo, Coutinho, Pedro M., Stam, Mark, Kallas, Åsa, Djerbi, Soraya, ...
Wood formation is a fundamental biological process with significant economic interest. While lignin biosynthesis is currently relatively well understood, the pathways leading to the synthesis of the...
N-linked glycosylation of native and recombinant cauliflower xyloglucan endotransglycosylase 16A.
Henriksson, Hongbin, Denman, Stuart E, Campuzano, Iain D G, Ademark, Pia, Master, Emma R, Teeri, Tuula T, ...
The gene encoding a XET (xyloglucan endotransglycosylase) from cauliflower ( Brassica oleracea var. botrytis ) florets has been cloned and sequenced. Sequence analysis indicated a high degree of...
Gene discovery in the wood-forming tissues of poplar: Analysis of 5,692 expressed sequence tags
Sterky, Fredrik, Regan, Sharon, Karlsson, Jan, Hertzberg, Magnus, Rohde, Antje, Holmberg, Anders, ...
A rapidly growing area of genome research is the generation of expressed sequence tags (ESTs) in which large numbers of randomly selected cDNA clones are partially sequenced. The collection of ESTs...
A transcriptional roadmap to wood formation
Hertzberg, Magnus, Aspeborg, Henrik, Schrader, Jarmo, Andersson, Anders, Erlandsson, Rikard, Blomqvist, Kristina, ...
The large vascular meristem of poplar trees with its highly organized secondary xylem enables the boundaries between different developmental zones to be easily distinguished. This property of...
Vanden Wymelenberg, Amber, Denman, Stuart, Dietrich, Diane, Bassett, Jennifer, Yu, Xiaochun, Atalla, Rajai, ...
Phanerochaete chrysosporium cellulase genes were cloned and characterized. The cel61A product was structurally similar to fungal endoglucanases of glycoside hydrolase family 61, whereas the cel9A...
The binding specificity and affinity determinants of family 1 and family 3 cellulose binding modules
Lehtiö, Janne, Sugiyama, Junji, Gustavsson, Malin, Fransson, Linda, Linder, Markus, Teeri, Tuula T.
Cellulose binding modules (CBMs) potentiate the action of cellulolytic enzymes on insoluble substrates. Numerous studies have established that three aromatic residues on a CBM surface are needed for...
Johansson, Patrik, Brumer, Harry, Baumann, Martin J., Kallas, Åsa M., Henriksson, Hongbin, Denman, Stuart E., ...
Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Thus, XET is a key enzyme in all plant processes that require...
Expansins Abundant in Secondary Xylem Belong to Subgroup A of the α-Expansin Gene Family1[w]
Gray-Mitsumune, Madoka, Mellerowicz, Ewa J., Abe, Hisashi, Schrader, Jarmo, Winzéll, Anders, Sterky, Fredrik, ...
Differentiation of xylem cells in dicotyledonous plants involves expansion of the radial primary cell walls and intrusive tip growth of cambial derivative cells prior to the deposition of a thick...
Carbohydrate-Active Enzymes Involved in the Secondary Cell Wall Biogenesis in Hybrid Aspen1
Aspeborg, Henrik, Schrader, Jarmo, Coutinho, Pedro M., Stam, Mark, Kallas, Åsa, Djerbi, Soraya, ...
Wood formation is a fundamental biological process with significant economic interest. While lignin biosynthesis is currently relatively well understood, the pathways leading to the synthesis of the...
N-linked glycosylation of native and recombinant cauliflower xyloglucan endotransglycosylase 16A.
Henriksson, Hongbin, Denman, Stuart E, Campuzano, Iain D G, Ademark, Pia, Master, Emma R, Teeri, Tuula T, ...
The gene encoding a XET (xyloglucan endotransglycosylase) from cauliflower ( Brassica oleracea var. botrytis ) florets has been cloned and sequenced. Sequence analysis indicated a high degree of...
Poplar Carbohydrate-Active Enzymes. Gene Identification and Expression Analyses1[W]
Geisler-Lee, Jane, Geisler, Matt, Coutinho, Pedro M., Segerman, Bo, Nishikubo, Nobuyuki, Takahashi, Junko, ...
Over 1,600 genes encoding carbohydrate-active enzymes (CAZymes) in the Populus trichocarpa (Torr. & Gray) genome were identified based on sequence homology, annotated, and grouped into families of...
Baumann, Martin J., Eklöf, Jens M., Michel, Gurvan, Kallas, Åsa M., Teeri, Tuula T., Czjzek, Mirjam, ...
High-resolution, three-dimensional structures of the archetypal glycoside hydrolase family 16 (GH16) endo-xyloglucanases Tm-NXG1 and Tm-NXG2 from nasturtium (Tropaeolum majus) have been solved by...
Urbanowicz, Breeanna R., Bennett, Alan B., Del Campillo, Elena, Catalá, Carmen, Hayashi, Takahisa, Henrissat, Bernard, ...
Rajangam, Alex S., Kumar, Manoj, Aspeborg, Henrik, Guerriero, Gea, Arvestad, Lars, Pansri, Podjamas, ...
We have identified a gene, denoted PttMAP20, which is strongly up-regulated during secondary cell wall synthesis and tightly coregulated with the secondary wall-associated CESA genes in hybrid aspen...