Self-assembly of S-layer-enveloped cytochrome c polyelectrolyte multilayers (2008)
Dronov, R., Kurth, D. G., Möhwald, H., Scheller, F. W., Friedmann, J., Pum, D., ...
T. Pavkov, E.M. Egelseer, M. Tesarz, D.I. Svergun, U.B. Sleytr, ...
Journal article
The structure and binding behavior of the bacterial cell surface layer protein SbsC (2008)
T. Pavkov, E.M. Egelseer, M. Tesarz, D.I. Svergun, U.B. Sleytr, ...
Journal article
Bacterial protein patterning by micro-contact printing of PLL-g-PEG (2007)
Saravia, V., Kupcu, S., Nolte, M., Huber, C., Pum, D., Fery, A., ...
Völlenkle, C., Weigert, S., Ilk, N., Egelseer, E., Weber, V., Loth, F., ...
The chimeric gene encoding a C-terminally-truncated form of the S-layer protein SbpA from Bacillus sphaericus CCM 2177 and two copies of the Fc-binding Z-domain was constructed, cloned, and...
Francis, L., Reekmans, G., De Palma, R., Campitelli, A., Sleytr, U. B.
We present results from an instrument combining surface acoustic wave (SAW) propagation and surface plasmon resonance (SPR) measurements. The objective is to use two independent methods, the former...
Increased protein coverage on biosensor surfaces using porous (2003)
Bonroy, Kristien; U0038701, Friedt, Jean-Michel; U0037367, Choi, Kang-Hoon, Frederix, Filip; U0032313, Laureyn, Wim, Campitelli, Andrew, ...
De Palma, Randy; U0041925, Friedt, Jean-Michel; U0037367, Francis, Laurent, Laureyn, Wim, Campitelli, Andrew, Sleytr, U.B, ...
Mass sensitivity calibration of love mode surface acoustic wave sensors for immunosensing (2003)
De Palma, Randy; U0041925, Francis, Laurent, Friedt, Jean-Michel; U0037367, Laureyn, Wim, Campitelli, Andrew, Sleytr, U.B, ...
Weygand, M., Kjaer, K., Howes, P.B., Wetzer, B., Pum, D., Sleytr, U.B., ...
Lateral Diffusion of Lipids in Silane-, Dextran- and S-layer-Supported Mono- and Bilayers (1999)
Györvary, E., Wetzer, B., Sleytr, U. B., Sinner, A., Offenhäusser, Andreas, Knoll, Wolfgang
Kadurugamuwa, J. L., Mayer, A., Messner, P., Sára, M., Sleytr, U. B., Beveridge, T. J.
When S-layered strains of Bacillus stearothermophilus and Aneurinibacillus thermoaerophilus, possessing S-layers of different lattice type and lattice constant as well as S-(glyco)protein chemistry,...
Egelseer, E, Schocher, I, Sára, M, Sleytr, U B
The S-layer lattice from Bacillus stearothermophilus DSM 2358 completely covers the cell surface and exhibits oblique symmetry. During growth of B. stearothermophilus DSM 2358 on starch medium, three...
Sára, M, Kuen, B, Mayer, H F, Mandl, F, Schuster, K C, Sleytr, U B
Stable synthesis of the hexagonally ordered (p6) S-layer protein from the wild-type strain of Bacillus stearothermophilus PV72 could be achieved in continuous culture on complex medium only under...
Egelseer, E M, Schocher, I, Sleytr, U B, Sára, M
During growth on starch medium, the S-layer-carrying Bacillus stearothermophilus ATCC 12980 and an S-layer-deficient variant each secreted three amylases, with identical molecular weights of 58,000,...
Ries, W, Hotzy, C, Schocher, I, Sleytr, U B, Sára, M
The S-layer of Bacillus stearothermophilus PV72/p2 shows oblique lattice symmetry and is composed of identical protein subunits with a molecular weight of 97,000. The isolated S-layer subunits could...
Nomellini, J F, Kupcu, S, Sleytr, U B, Smit, J
The S-layer of Caulobacter is a two-dimensional paracrystalline array on the cell surface composed of a single protein, RsaA. We have established conditions for preparation of stable, soluble protein...
Paracrystalline surface layers of dairy propionibacteria.
Lortal, S, Rouault, A, Cesselin, B, Sleytr, U B
We examined 70 dairy propionibacteria and detected a crystalline surface layer (S-layer) in only 2 organisms (Propionibacterium freudenreichii CNRZ 722 and Propionibacterium jensenii CNRZ 87) by...
Christian, R, Schulz, G, Schuster-Kolbe, J, Allmaier, G, Schmid, E R, Sleytr, U B, ...
In this study, we have extended and completed a previous investigation (P. Messner, R. Christian, J. Kolbe, G. Schulz, and U. B. Sleytr, J. Bacteriol. 174:2236-2240, 1992) in which we demonstrated...
The specific properties of S-layer proteins from three different Bacillus stearothermophilus strains revealing oblique, square, or hexagonal lattice symmetry were preserved during growth in...
In this paper, the importance of charged amino and carboxyl groups for the integrity of the cell surface layer (S-layer) lattice from Bacillus coagulans E38-66 and for the self-assembly of the...
Pum, D, Weinhandl, M, Hödl, C, Sleytr, U B
S-layer protein isolated from Bacillus coagulans E38-66 could be recrystallized into large-scale coherent monolayers at an air/water interface and on phospholipid films spread on a Langmuir-Blodgett...
Sára, M, Pum, D, Küpcü, S, Messner, P, Sleytr, U B
During growth of Bacillus stearothermophilus NRS 2004/3a in continuous culture on complex medium, the chemical properties of the S-layer glycoprotein and the characteristic oblique lattice were...
Messner, P, Christian, R, Kolbe, J, Schulz, G, Sleytr, U B
The surface layer glycoprotein of Clostridium thermohydrosulfuricum S102-70 was shown to contain a new type of glycan chain. Different from all known eubacterial glycoproteins, the saccharide moiety...
We investigated the permeability properties of the oblique S-layer lattice from Bacillus coagulans E38-66 after depositing cell wall fragments on a microfiltration membrane, cross-linking the S-layer...
Evidence for an S-layer protein pool in the peptidoglycan of Bacillus stearothermophilus.
Breitwieser, A, Gruber, K, Sleytr, U B
Intact cells of Bacillus stearothermophilus PV72 revealed, after conventional thin-sectioning procedures, the typical cell wall profile of S-layer-carrying gram-positive eubacteria consisting of a...
Characterization of the surface layer glycoprotein of Clostridium symbiosum HB25.
Messner, P, Bock, K, Christian, R, Schulz, G, Sleytr, U B
The cell surface of Clostridium symbiosum HB25 is covered by a squarely arranged surface layer (S-layer) glycoprotein. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the sodium dodecyl...
Pum, D, Messner, P, Sleytr, U B
Thin sections, freeze-etched, and negatively stained preparations of Methanocorpusculum sinense cells reveal a highly lobed cell structure with a hexagonally arranged surface layer (S layer). Digital...
Structure, surface charge, and self-assembly of the S-layer lattice from Bacillus coagulans E38-66.
In freeze-etched preparations, whole cells from Bacillus coagulans E38-66 exhibited an oblique S-layer lattice (a = 9.4 nm; b = 7.4 nm; gamma = 80 degrees). The three-dimensional structure of the...
The distribution and functional significance of charged groups on the outer and inner faces of the S layer from Bacillus stearothermophilus NRS 1536/3c was investigated. Chemical modification of the...
Molecular sieving through S layers of Bacillus stearothermophilus strains.
The permeability properties and the exclusion limits of the crystalline surface layers (S layers) of two selected strains of Bacillus stearothermophilus were investigated. Measurements were performed...
Messner, P, Pum, D, Sára, M, Stetter, K O, Sleytr, U B
The ultrastructures of the regular surface layers (S-layers) of the extremely thermophilic archaebacteria Thermoproteus tenax and Thermoproteus neutrophilus were examined by freeze-etching,...
Sleytr, U B, Shockman, G D, Higgins, M L
The proposed lower internal density of the gram-positive wall was confirmed by observed an internal fracture plane in the walls of Streptococcus faecalis and Streptococcus mutans. However, the...
Cell walls of Clostridium thermohydrosulfuricum and C. thermosaccharolyticum have a two-layered structure, consisting of a thin, lysozyme-sensitive murein layer and a surface (S) layer composed of...
Clostridum thermosaccharolyticum and Clostridium thermohydrosulfuricum possess as outermost cell wall layer a tetragonal or hexagonal ordered array of macromolecules. The subunits of the surface...
Sleytr, U. B., Thornley, Margaret J.
Freeze-etching was applied to preparations, with and without glycerol, of Acinetobacter sp. strain MJT/F5/199A, consisting of intact cells after normal growth or after incubation with...
Location of the Fracture Faces Within the Cell Envelope of Acinetobacter Species Strain MJT/F5/5
Sleytr, U. B., Thornley, Margaret J., Glauert, Audrey M.
The cell wall of the gram-negative bacterium Acinetobacter species strain MJT/F5/5 shows in thin section an external “additional” layer, an outer membrane, an intermediate layer, and a dense...
Isolation of Outer Membranes with an Ordered Array of Surface Subunits from Acinetobacter
Thornley, Margaret J., Glauert, Audrey M., Sleytr, U. B.
A method has been developed for the isolation of outer membranes from Acinetobacter sp. strain MJT/F5/199A. Washed cells were broken in a French press and, after deoxyribonuclease and ribonuclease...
Higgins, M L, McDowell, T D, Sleytr, U B, Mychajlonka, M, Shockman, G D
Strains of Streptococcus mutans are very susceptible to growth inhibition by benzylpenicillin, but are tolerant to lysis when exposed to even high concentrations of this drug. These properties...
Hirn, R, Schuster, B, Sleytr, U B, Bayerl, T M
A dedicated dynamic light scattering (DLS) setup was employed to study the undulations of freely suspended planar lipid bilayers, the so-called black lipid membranes (BLM), over a previously...
Weygand, M, Wetzer, B, Pum, D, Sleytr, U B, Cuvillier, N, Kjaer, K, ...
The coupling of bacterial surface (S)-layer proteins to lipid membranes is studied in molecular detail for proteins from Bacillus sphaericus CCM2177 and B. coagulans E38-66 recrystallized at...
Kadurugamuwa, J. L., Mayer, A., Messner, P., Sára, M., Sleytr, U. B., Beveridge, T. J.
When S-layered strains of Bacillus stearothermophilus and Aneurinibacillus thermoaerophilus, possessing S-layers of different lattice type and lattice constant as well as S-(glyco)protein chemistry,...
Egelseer, E, Schocher, I, Sára, M, Sleytr, U B
The S-layer lattice from Bacillus stearothermophilus DSM 2358 completely covers the cell surface and exhibits oblique symmetry. During growth of B. stearothermophilus DSM 2358 on starch medium, three...
Sára, M, Kuen, B, Mayer, H F, Mandl, F, Schuster, K C, Sleytr, U B
Stable synthesis of the hexagonally ordered (p6) S-layer protein from the wild-type strain of Bacillus stearothermophilus PV72 could be achieved in continuous culture on complex medium only under...
Egelseer, E M, Schocher, I, Sleytr, U B, Sára, M
During growth on starch medium, the S-layer-carrying Bacillus stearothermophilus ATCC 12980 and an S-layer-deficient variant each secreted three amylases, with identical molecular weights of 58,000,...
Ries, W, Hotzy, C, Schocher, I, Sleytr, U B, Sára, M
The S-layer of Bacillus stearothermophilus PV72/p2 shows oblique lattice symmetry and is composed of identical protein subunits with a molecular weight of 97,000. The isolated S-layer subunits could...
Nomellini, J F, Kupcu, S, Sleytr, U B, Smit, J
The S-layer of Caulobacter is a two-dimensional paracrystalline array on the cell surface composed of a single protein, RsaA. We have established conditions for preparation of stable, soluble protein...
Paracrystalline surface layers of dairy propionibacteria.
Lortal, S, Rouault, A, Cesselin, B, Sleytr, U B
We examined 70 dairy propionibacteria and detected a crystalline surface layer (S-layer) in only 2 organisms (Propionibacterium freudenreichii CNRZ 722 and Propionibacterium jensenii CNRZ 87) by...
Christian, R, Schulz, G, Schuster-Kolbe, J, Allmaier, G, Schmid, E R, Sleytr, U B, ...
In this study, we have extended and completed a previous investigation (P. Messner, R. Christian, J. Kolbe, G. Schulz, and U. B. Sleytr, J. Bacteriol. 174:2236-2240, 1992) in which we demonstrated...
The specific properties of S-layer proteins from three different Bacillus stearothermophilus strains revealing oblique, square, or hexagonal lattice symmetry were preserved during growth in...
In this paper, the importance of charged amino and carboxyl groups for the integrity of the cell surface layer (S-layer) lattice from Bacillus coagulans E38-66 and for the self-assembly of the...
Pum, D, Weinhandl, M, Hödl, C, Sleytr, U B
S-layer protein isolated from Bacillus coagulans E38-66 could be recrystallized into large-scale coherent monolayers at an air/water interface and on phospholipid films spread on a Langmuir-Blodgett...
Sára, M, Pum, D, Küpcü, S, Messner, P, Sleytr, U B
During growth of Bacillus stearothermophilus NRS 2004/3a in continuous culture on complex medium, the chemical properties of the S-layer glycoprotein and the characteristic oblique lattice were...
Messner, P, Christian, R, Kolbe, J, Schulz, G, Sleytr, U B
The surface layer glycoprotein of Clostridium thermohydrosulfuricum S102-70 was shown to contain a new type of glycan chain. Different from all known eubacterial glycoproteins, the saccharide moiety...
We investigated the permeability properties of the oblique S-layer lattice from Bacillus coagulans E38-66 after depositing cell wall fragments on a microfiltration membrane, cross-linking the S-layer...
Evidence for an S-layer protein pool in the peptidoglycan of Bacillus stearothermophilus.
Breitwieser, A, Gruber, K, Sleytr, U B
Intact cells of Bacillus stearothermophilus PV72 revealed, after conventional thin-sectioning procedures, the typical cell wall profile of S-layer-carrying gram-positive eubacteria consisting of a...
Characterization of the surface layer glycoprotein of Clostridium symbiosum HB25.
Messner, P, Bock, K, Christian, R, Schulz, G, Sleytr, U B
The cell surface of Clostridium symbiosum HB25 is covered by a squarely arranged surface layer (S-layer) glycoprotein. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the sodium dodecyl...
Pum, D, Messner, P, Sleytr, U B
Thin sections, freeze-etched, and negatively stained preparations of Methanocorpusculum sinense cells reveal a highly lobed cell structure with a hexagonally arranged surface layer (S layer). Digital...
Structure, surface charge, and self-assembly of the S-layer lattice from Bacillus coagulans E38-66.
In freeze-etched preparations, whole cells from Bacillus coagulans E38-66 exhibited an oblique S-layer lattice (a = 9.4 nm; b = 7.4 nm; gamma = 80 degrees). The three-dimensional structure of the...
The distribution and functional significance of charged groups on the outer and inner faces of the S layer from Bacillus stearothermophilus NRS 1536/3c was investigated. Chemical modification of the...
Molecular sieving through S layers of Bacillus stearothermophilus strains.
The permeability properties and the exclusion limits of the crystalline surface layers (S layers) of two selected strains of Bacillus stearothermophilus were investigated. Measurements were performed...
Messner, P, Pum, D, Sára, M, Stetter, K O, Sleytr, U B
The ultrastructures of the regular surface layers (S-layers) of the extremely thermophilic archaebacteria Thermoproteus tenax and Thermoproteus neutrophilus were examined by freeze-etching,...
Sleytr, U B, Shockman, G D, Higgins, M L
The proposed lower internal density of the gram-positive wall was confirmed by observed an internal fracture plane in the walls of Streptococcus faecalis and Streptococcus mutans. However, the...
Cell walls of Clostridium thermohydrosulfuricum and C. thermosaccharolyticum have a two-layered structure, consisting of a thin, lysozyme-sensitive murein layer and a surface (S) layer composed of...
Clostridum thermosaccharolyticum and Clostridium thermohydrosulfuricum possess as outermost cell wall layer a tetragonal or hexagonal ordered array of macromolecules. The subunits of the surface...
Sleytr, U. B., Thornley, Margaret J.
Freeze-etching was applied to preparations, with and without glycerol, of Acinetobacter sp. strain MJT/F5/199A, consisting of intact cells after normal growth or after incubation with...
Location of the Fracture Faces Within the Cell Envelope of Acinetobacter Species Strain MJT/F5/5
Sleytr, U. B., Thornley, Margaret J., Glauert, Audrey M.
The cell wall of the gram-negative bacterium Acinetobacter species strain MJT/F5/5 shows in thin section an external “additional” layer, an outer membrane, an intermediate layer, and a dense...
Isolation of Outer Membranes with an Ordered Array of Surface Subunits from Acinetobacter
Thornley, Margaret J., Glauert, Audrey M., Sleytr, U. B.
A method has been developed for the isolation of outer membranes from Acinetobacter sp. strain MJT/F5/199A. Washed cells were broken in a French press and, after deoxyribonuclease and ribonuclease...
Higgins, M L, McDowell, T D, Sleytr, U B, Mychajlonka, M, Shockman, G D
Strains of Streptococcus mutans are very susceptible to growth inhibition by benzylpenicillin, but are tolerant to lysis when exposed to even high concentrations of this drug. These properties...
Hirn, R, Schuster, B, Sleytr, U B, Bayerl, T M
A dedicated dynamic light scattering (DLS) setup was employed to study the undulations of freely suspended planar lipid bilayers, the so-called black lipid membranes (BLM), over a previously...
Weygand, M, Wetzer, B, Pum, D, Sleytr, U B, Cuvillier, N, Kjaer, K, ...
The coupling of bacterial surface (S)-layer proteins to lipid membranes is studied in molecular detail for proteins from Bacillus sphaericus CCM2177 and B. coagulans E38-66 recrystallized at...