Role of Ets-1 in erythroid differentiation (2002)
Marziali, G., Perrotti, E., Ilari, R., Lulli, V., Coccia, E. M., Mazzeo, S., ...
Members of the Ets gene family are known to be expressed in the hematopoietic tissue and some of them play a pivotal role in normal hematopoietic cell development. Ets-1 gene expression was analyzed...
Marziali, G., Perrotti, E., Ilari, R., Lulli, V., Coccia, E. M., Moret, R., ...
High expression of transferrin receptor (TfR) on the membrane of erythroid cells accounts for the high level of iron required to sustain heme synthesis. Several studies indicate that during erythroid...
Mechanisms of differential transferrin receptor expression in normal hematopoiesis (2000)
Sposi, N. M., Cianetti, L., Tritarelli, E., Pelosi, E., Militi, S., Barberi, T., ...
We have investigated the expression of transferrin receptor (TfR) iron regulatory protein-1 (IRP-1) and iron regulatory protein-2 (IRP-2) in liquid suspension culture of purified hematopoietic...
Post-transcriptional regulation of transferrin receptor mRNA by IFN gamma (1992)
Bourgeade, M. F., Silbermann, F., Kühn, L. C., Testa, U., Peschle, C., Memet, S., ...
IFN gamma inhibits the rise in transferrin receptor mRNA level which is normally observed when stationary WISH cells are stimulated to proliferate. This effect is not attributable to a change in the...
Testa, U., Kühn, L. C., Petrini, M., Quaranta, M. T., Pelosi, E., Peschle, C.
The intracellular iron level exerts a negative feedback on transferrin receptor (TfR) expression in cells requiring iron for their proliferation, in contrast to the positive feedback observed in...
Condorelli, G L, Testa, U, Valtieri, M, Vitelli, L, De Luca, A, Barberi, T, ...
The retinoblastoma (RB) gene specifies a nuclear phosphoprotein (pRb 105), which is a prototype tumor suppressor inactivated in a variety of human tumors. Recent studies suggest that RB is also...
Sposi, N M, Zon, L I, Carè, A, Valtieri, M, Testa, U, Gabbianelli, M, ...
The programmed activation/repression of transcription factors in early hematopoietic differentiation has not yet been explored. The DNA-binding protein GATA-1 is required for normal erythroid...
Marziali, G, Perrotti, E, Ilari, R, Testa, U, Coccia, E M, Battistini, A
The ferritin H-chain gene promoter regulation was analyzed in heme-treated Friend leukemia cells (FLCs) and during monocyte-to-macrophage differentiation. In the majority of cell lines studied, the...
Condorelli, G L, Tocci, A, Botta, R, Facchiano, F, Testa, U, Vitelli, L, ...
The TAL-1 gene specifies a basic helix-loop-helix domain (bHLH) transcription factor, which heterodimerizes with E2A gene family proteins. tal-1 protein is abnormally expressed in the majority of...
Ruthardt, M, Testa, U, Nervi, C, Ferrucci, P F, Grignani, F, Puccetti, E, ...
Fusion proteins involving the retinoic acid receptor alpha (RAR alpha) and the PML or PLZF nuclear protein are the genetic markers of acute promyelocytic leukemias (APLs). APLs with the PML-RAR alpha...
Locardi, C, Petrini, C, Boccoli, G, Testa, U, Diefenbach, C, Buttò, S, ...
Locardi, C, Petrini, C, Boccoli, G, Testa, U, Dieffenbach, C, Buttò, S, ...
We have investigated the roles of cytokines in the modulation of human immunodeficiency virus (HIV) production in chronically infected U937 cells upon in vitro differentiation by hydroxyvitamin D3....
Labbaye, C, Valtieri, M, Barberi, T, Meccia, E, Masella, B, Pelosi, E, ...
We have explored the expression of the transcription factors GATA-1, GATA-2, and NF-E2 in purified early hematopoietic progenitor cells (HPCs) induced to gradual unilineage erythroid or granulocytic...
Migliaccio, G, Migliaccio, A R, Petti, S, Mavilio, F, Russo, G, Lazzaro, D, ...
Human embryonic development involves transition from yolk sac (YS) to liver (L) hemopoiesis. We report the identification of pluripotent, erythroid, and granulo-macrophage progenitors in YS, L, and...
Molecular mechanisms underlying the expression of the human HOX-5.1 gene.
Cianetti, L, Di Cristofaro, A, Zappavigna, V, Bottero, L, Boccoli, G, Testa, U, ...
The complex mechanisms underlying homeobox genes expression involve regulation at transcriptional, post-transcriptional and translational levels. The multiple transcripts of the human HOX-5.1 gene...
Coordinate expression and proliferative role of HOXB genes in activated adult T lymphocytes.
Carè, A, Testa, U, Bassani, A, Tritarelli, E, Montesoro, E, Samoggia, P, ...
We investigated the expression of HOXB cluster genes in purified phytohemagglutinin (PHA)-activated T lymphocytes from normal adult peripheral blood by reverse transcription PCR and RNase protection....
Expression of protein kinase C genes during ontogenic development of the central nervous system.
Sposi, N M, Bottero, L, Cossu, G, Russo, G, Testa, U, Peschle, C
We have analyzed the RNA expression of three protein kinase C (PKC) genes (alpha, beta, and gamma) in human and murine central nervous systems during embryonic-fetal, perinatal, and adult life....
Coccia, E M, Profita, V, Fiorucci, G, Romeo, G, Affabris, E, Testa, U, ...
The mechanisms that regulate the expression of the H chain of the iron storage protein ferritin in Friend erythroleukemia cells (FLCs) after exposure to hemin (ferric protoporphyrin IX),...
Liboi, E, Pelosi, E, Testa, U, Peschle, C, Rossi, G B
Extensive evidence supports a two-step model for the control of fibroblast growth, which includes first the action of a competence factor (e.g., platelet-derived growth factor) followed by the...
Modiano, G, Battistuzzi, G, Esan, G J, Testa, U, Luzzatto, L
Quantitative determination of glucose-6-phosphate dehydrogenase (G6PD; D-glucose-6-phosphate: NADP+ 1-oxidoreductase, EC 1.1.1.49) activity was carried out in 214 male Nigerian children of 84 mothers...
Presence of the Tn antigen on hematopoietic progenitors from patients with the Tn syndrome.
Vainchenker, W, Vinci, G, Testa, U, Henri, A, Tabilio, A, Fache, M P, ...
The Tn syndrome is an acquired clonal disorder characterized by the exposure of a normally hidden determinant, the Tn antigen, on the surface of human erythrocytes, platelets, granulocytes, and...
Grignani, F, Testa, U, Rogaia, D, Ferrucci, P F, Samoggia, P, Pinto, A, ...
The block of terminal differentiation is a prominent feature of acute promyelocytic leukemia (APL) and its release by retinoic acid correlates with disease remission. Expression of the APL-specific...
Testa, U, Grignani, F, Samoggia, P, Zanetti, C, Riccioni, R, Lo Coco, F, ...
We investigated the effect of the acute promyelocytic leukemia (APL) specific PML/RARalpha fusion protein on the sensitivity to TNF-alpha-mediated apoptosis. The U937 leukemia cell line was...
Tabilio, A, Rosa, J P, Testa, U, Kieffer, N, Nurden, A T, Del Canizo, M C, ...
We demonstrate that HEL, a human erythroleukemic cell line, has numerous megakaryocytic markers which were markedly enhanced following the addition of the inducers dimethyl sulfoxide or...
Matarrese, P, Testa, U, Cauda, R, Vella, S, Gambardella, L, Malorni, W
Multidrug resistance caused by P-glycoprotein (P-170) is a phenomenon by which cells exposed to a single drug acquire resistance to other structurally and functionally unrelated drugs. This is a...
Ectopic expression of interferon regulatory factor-1 potentiates granulocytic differentiation.
Coccia, E M, Stellacci, E, Valtieri, M, Masella, B, Feccia, T, Marziali, G, ...
Numerous transcription factors allow haematopoietic cells to respond to lineage- and stage-specific cytokines and to act as their effectors. It is increasingly evident that the interferon regulatory...
Post-transcriptional regulation of transferrin receptor mRNA by IFN gamma.
Bourgeade, M F, Silbermann, F, Kühn, L, Testa, U, Peschle, C, Mémet, S, ...
IFN gamma inhibits the rise in transferrin receptor mRNA level which is normally observed when stationary WISH cells are stimulated to proliferate. This effect is not attributable to a change in the...
Condorelli, G L, Testa, U, Valtieri, M, Vitelli, L, De Luca, A, Barberi, T, ...
The retinoblastoma (RB) gene specifies a nuclear phosphoprotein (pRb 105), which is a prototype tumor suppressor inactivated in a variety of human tumors. Recent studies suggest that RB is also...
Sposi, N M, Zon, L I, Carè, A, Valtieri, M, Testa, U, Gabbianelli, M, ...
The programmed activation/repression of transcription factors in early hematopoietic differentiation has not yet been explored. The DNA-binding protein GATA-1 is required for normal erythroid...
Marziali, G, Perrotti, E, Ilari, R, Testa, U, Coccia, E M, Battistini, A
The ferritin H-chain gene promoter regulation was analyzed in heme-treated Friend leukemia cells (FLCs) and during monocyte-to-macrophage differentiation. In the majority of cell lines studied, the...
Condorelli, G L, Tocci, A, Botta, R, Facchiano, F, Testa, U, Vitelli, L, ...
The TAL-1 gene specifies a basic helix-loop-helix domain (bHLH) transcription factor, which heterodimerizes with E2A gene family proteins. tal-1 protein is abnormally expressed in the majority of...
Ruthardt, M, Testa, U, Nervi, C, Ferrucci, P F, Grignani, F, Puccetti, E, ...
Fusion proteins involving the retinoic acid receptor alpha (RAR alpha) and the PML or PLZF nuclear protein are the genetic markers of acute promyelocytic leukemias (APLs). APLs with the PML-RAR alpha...
Locardi, C, Petrini, C, Boccoli, G, Testa, U, Diefenbach, C, Buttò, S, ...
Locardi, C, Petrini, C, Boccoli, G, Testa, U, Dieffenbach, C, Buttò, S, ...
We have investigated the roles of cytokines in the modulation of human immunodeficiency virus (HIV) production in chronically infected U937 cells upon in vitro differentiation by hydroxyvitamin D3....
Labbaye, C, Valtieri, M, Barberi, T, Meccia, E, Masella, B, Pelosi, E, ...
We have explored the expression of the transcription factors GATA-1, GATA-2, and NF-E2 in purified early hematopoietic progenitor cells (HPCs) induced to gradual unilineage erythroid or granulocytic...
Post-transcriptional regulation of transferrin receptor mRNA by IFN gamma.
Bourgeade, M F, Silbermann, F, Kühn, L, Testa, U, Peschle, C, Mémet, S, ...
IFN gamma inhibits the rise in transferrin receptor mRNA level which is normally observed when stationary WISH cells are stimulated to proliferate. This effect is not attributable to a change in the...
Migliaccio, G, Migliaccio, A R, Petti, S, Mavilio, F, Russo, G, Lazzaro, D, ...
Human embryonic development involves transition from yolk sac (YS) to liver (L) hemopoiesis. We report the identification of pluripotent, erythroid, and granulo-macrophage progenitors in YS, L, and...
Molecular mechanisms underlying the expression of the human HOX-5.1 gene.
Cianetti, L, Di Cristofaro, A, Zappavigna, V, Bottero, L, Boccoli, G, Testa, U, ...
The complex mechanisms underlying homeobox genes expression involve regulation at transcriptional, post-transcriptional and translational levels. The multiple transcripts of the human HOX-5.1 gene...
Coordinate expression and proliferative role of HOXB genes in activated adult T lymphocytes.
Carè, A, Testa, U, Bassani, A, Tritarelli, E, Montesoro, E, Samoggia, P, ...
We investigated the expression of HOXB cluster genes in purified phytohemagglutinin (PHA)-activated T lymphocytes from normal adult peripheral blood by reverse transcription PCR and RNase protection....
Expression of protein kinase C genes during ontogenic development of the central nervous system.
Sposi, N M, Bottero, L, Cossu, G, Russo, G, Testa, U, Peschle, C
We have analyzed the RNA expression of three protein kinase C (PKC) genes (alpha, beta, and gamma) in human and murine central nervous systems during embryonic-fetal, perinatal, and adult life....
Coccia, E M, Profita, V, Fiorucci, G, Romeo, G, Affabris, E, Testa, U, ...
The mechanisms that regulate the expression of the H chain of the iron storage protein ferritin in Friend erythroleukemia cells (FLCs) after exposure to hemin (ferric protoporphyrin IX),...
Liboi, E, Pelosi, E, Testa, U, Peschle, C, Rossi, G B
Extensive evidence supports a two-step model for the control of fibroblast growth, which includes first the action of a competence factor (e.g., platelet-derived growth factor) followed by the...
Modiano, G, Battistuzzi, G, Esan, G J, Testa, U, Luzzatto, L
Quantitative determination of glucose-6-phosphate dehydrogenase (G6PD; D-glucose-6-phosphate: NADP+ 1-oxidoreductase, EC 1.1.1.49) activity was carried out in 214 male Nigerian children of 84 mothers...
Presence of the Tn antigen on hematopoietic progenitors from patients with the Tn syndrome.
Vainchenker, W, Vinci, G, Testa, U, Henri, A, Tabilio, A, Fache, M P, ...
The Tn syndrome is an acquired clonal disorder characterized by the exposure of a normally hidden determinant, the Tn antigen, on the surface of human erythrocytes, platelets, granulocytes, and...
Grignani, F, Testa, U, Rogaia, D, Ferrucci, P F, Samoggia, P, Pinto, A, ...
The block of terminal differentiation is a prominent feature of acute promyelocytic leukemia (APL) and its release by retinoic acid correlates with disease remission. Expression of the APL-specific...
Testa, U, Grignani, F, Samoggia, P, Zanetti, C, Riccioni, R, Lo Coco, F, ...
We investigated the effect of the acute promyelocytic leukemia (APL) specific PML/RARalpha fusion protein on the sensitivity to TNF-alpha-mediated apoptosis. The U937 leukemia cell line was...
Tabilio, A, Rosa, J P, Testa, U, Kieffer, N, Nurden, A T, Del Canizo, M C, ...
We demonstrate that HEL, a human erythroleukemic cell line, has numerous megakaryocytic markers which were markedly enhanced following the addition of the inducers dimethyl sulfoxide or...
Matarrese, P, Testa, U, Cauda, R, Vella, S, Gambardella, L, Malorni, W
Multidrug resistance caused by P-glycoprotein (P-170) is a phenomenon by which cells exposed to a single drug acquire resistance to other structurally and functionally unrelated drugs. This is a...
Ectopic expression of interferon regulatory factor-1 potentiates granulocytic differentiation.
Coccia, E M, Stellacci, E, Valtieri, M, Masella, B, Feccia, T, Marziali, G, ...
Numerous transcription factors allow haematopoietic cells to respond to lineage- and stage-specific cytokines and to act as their effectors. It is increasingly evident that the interferon regulatory...
Pelosi-Testa, E, Samoggia, P, Giannella, G, Montesoro, E, Caravita, T, Salvo, G, ...
Peripheral blood mononuclear cells (PBM) pulsed with lectin (PHA or Con A for 0.25-3 hr) show a low expression of interleukin-2 and transferrin receptors (IL-2Rs, TfRs) and a mild decline of...
Habetswallner, D., Pelosi, E., Bulgarini, D., Camagna, A., Samoggia, P., Montesoro, E., ...
Purified human T lymphocytes, completely depleted of accessory cells [i.e. monocytes, large granular lymphocytes (LGL) and B lymphocytes], have been grown in serum-free culture in presence of a...
Prognostic significance of interleukin 6 serum levels in patients with ovarian cancer.
Scambia, G., Testa, U., Benedetti Panici, P., Foti, E., Martucci, R., Gadducci, A., ...
High levels of IL-6 were found in 50% of 114 patients with primary ovarian cancer. IL-6 sensitivity was lower than that of CA 125, and the combination of both assays did not increase the sensitivity...