Ulf Gerth

Identification of ςB-Dependent Genes in Bacillus subtilis Using a Promoter Consensus-Directed Search and Oligonucleotide Hybridization

Petersohn, Anja, Bernhardt, Jörg, Gerth, Ulf, Höper, Dirk, Koburger, Torsten, Völker, Uwe, ...

A consensus-directed search for ςB promoters was used to locate potential candidates for new ςB-dependent genes in Bacillus subtilis. Screening of those candidates by oligonucleotide hybridizations...

ClpXP Protease Regulates the Signal Peptide Cleavage of Secretory Preproteins in Bacillus subtilis with a Mechanism Distinct from That of the Ecs ABC Transporter

Pummi, Tiina, Leskelä, Soile, Wahlström, Eva, Gerth, Ulf, Tjalsma, Harold, Hecker, Michael, ...

Identification and characterization of a suppressor mutation, sup-15, which partially restored secretion in the protein secretion-deficient Bacillus subtilis ecsA26 mutant, led us to discover a novel...

Fine-Tuning in Regulation of Clp Protein Content in Bacillus subtilis†

Gerth, Ulf, Kirstein, Janine, Mostertz, Jörg, Waldminghaus, Torsten, Miethke, Marcus, Kock, Holger, ...

Clp-controlled proteolysis in Bacillus subtilis seems to play a substantial role, particularly under stress conditions. Calibrated Western blot analyses were used to estimate the approximate numbers...

The ClpP Peptidase Is the Major Determinant of Bulk Protein Turnover in Bacillus subtilis

Kock, Holger, Gerth, Ulf, Hecker, Michael

Measurements of overall protein degradation rates in wild-type and clpP mutant Bacillus subtilis cells revealed that stress- or starvation-induced bulk protein turnover depends virtually exclusively...

A tyrosine kinase and its activator control the activity of the CtsR heat shock repressor in B. subtilis

Kirstein, Janine, Zühlke, Daniela, Gerth, Ulf, Turgay, Kürşad, Hecker, Michael

The soil bacterium Bacillus subtilis possesses a fine-tuned and complex heat stress response system. The repressor CtsR, whose activity is regulated by its modulators McsA and McsB, controls the...

Involvement of Bacillus subtilis ClpE in CtsR Degradation and Protein Quality Control

Miethke, Marcus, Hecker, Michael, Gerth, Ulf

The heat-inducible CtsR regulon of Bacillus subtilis codes for three Clp proteins with chaperone or protease activity. While the importance of ClpC and ClpP has been elucidated for a wide range of...

Identification of ςB-Dependent Genes in Bacillus subtilis Using a Promoter Consensus-Directed Search and Oligonucleotide Hybridization

Petersohn, Anja, Bernhardt, Jörg, Gerth, Ulf, Höper, Dirk, Koburger, Torsten, Völker, Uwe, ...

A consensus-directed search for ςB promoters was used to locate potential candidates for new ςB-dependent genes in Bacillus subtilis. Screening of those candidates by oligonucleotide hybridizations...

ClpXP Protease Regulates the Signal Peptide Cleavage of Secretory Preproteins in Bacillus subtilis with a Mechanism Distinct from That of the Ecs ABC Transporter

Pummi, Tiina, Leskelä, Soile, Wahlström, Eva, Gerth, Ulf, Tjalsma, Harold, Hecker, Michael, ...

Identification and characterization of a suppressor mutation, sup-15, which partially restored secretion in the protein secretion-deficient Bacillus subtilis ecsA26 mutant, led us to discover a novel...

Fine-Tuning in Regulation of Clp Protein Content in Bacillus subtilis†

Gerth, Ulf, Kirstein, Janine, Mostertz, Jörg, Waldminghaus, Torsten, Miethke, Marcus, Kock, Holger, ...

Clp-controlled proteolysis in Bacillus subtilis seems to play a substantial role, particularly under stress conditions. Calibrated Western blot analyses were used to estimate the approximate numbers...

The ClpP Peptidase Is the Major Determinant of Bulk Protein Turnover in Bacillus subtilis

Kock, Holger, Gerth, Ulf, Hecker, Michael

Measurements of overall protein degradation rates in wild-type and clpP mutant Bacillus subtilis cells revealed that stress- or starvation-induced bulk protein turnover depends virtually exclusively...

A tyrosine kinase and its activator control the activity of the CtsR heat shock repressor in B. subtilis

Kirstein, Janine, Zühlke, Daniela, Gerth, Ulf, Turgay, Kürşad, Hecker, Michael

The soil bacterium Bacillus subtilis possesses a fine-tuned and complex heat stress response system. The repressor CtsR, whose activity is regulated by its modulators McsA and McsB, controls the...

Involvement of Bacillus subtilis ClpE in CtsR Degradation and Protein Quality Control

Miethke, Marcus, Hecker, Michael, Gerth, Ulf

The heat-inducible CtsR regulon of Bacillus subtilis codes for three Clp proteins with chaperone or protease activity. While the importance of ClpC and ClpP has been elucidated for a wide range of...

ClpP Modulates the Activity of the Bacillus subtilis Stress Response Transcription Factor, σB▿

Reeves, Adam, Gerth, Ulf, Völker, Uwe, Haldenwang, W. G.

The general stress regulon of Bacillus subtilis is controlled by the activity state of σB, a transcription factor that is switched on following exposure to either physical or nutritional stress....

The tyrosine kinase McsB is a regulated adaptor protein for ClpCP

Kirstein, Janine, Dougan, David A, Gerth, Ulf, Hecker, Michael, Turgay, Kürşad

Cells of the soil bacterium Bacillus subtilis have to adapt to fast environmental changes in their natural habitat. Here, we characterized a novel system in which cells respond to heat shock by...

Clp-Dependent Proteolysis Down-Regulates Central Metabolic Pathways in Glucose-Starved Bacillus subtilis▿ †

Gerth, Ulf, Kock, Holger, Kusters, Ilja, Michalik, Stephan, Switzer, Robert L., Hecker, Michael

Entry into stationary phase in Bacillus subtilis is linked not only to a redirection of the gene expression program but also to posttranslational events such as protein degradation. Using 35S-labeled...