Massinen, Satu, Tammimies, Kristiina, Tapia-Páez, Isabel, Matsson, Hans, Hokkanen, Marie-Estelle, Söderberg, Ola, ...
Dyslexia, or specific reading disability, is the unexpected failure in learning to read and write when intelligence and senses are normal. One of the susceptibility genes, DYX1C1, has been implicated...
Jobs, Magnus, Eriksson, Ronnie, Ekstrand, Charlotta, Ullberg, Måns, Hermann, Björn, Landegren, Ulf, ...
A new concept for multiplex detection and quantification of microbes is here demonstrated on a range of infectious fungal species. Padlock probe methodology in conjunction with qPCR and Luminex™...
Estrogen receptor alpha gene polymorphism and endometrial cancer risk – a case-control study (2008)
Wedrén, Sara, Lovmar, Lovisa, Humphreys, Keith, Magnusson, Cecilia, Melhus, Håkan, Syvänen, Ann-Christine, ...
Abstract Background Estrogen is an established endometrial carcinogen. One of the most important mediators of estrogenic action is the estrogen receptor alpha. We have investigated whether...
Rietdorf, Katja, Kotelevets, Larissa, ...
Imbalance of signals that control cell survival and death results in pathologies, including cancer and neurodegeneration. Two pathways that are integral to setting the balance between cell survival...
A dual-tag microarray platform for high-performance nucleic acid and protein analyses (2008)
Ericsson, Olle, Jarvius, Jonas, Schallmeiner, Edith, Howell, Mathias, Nong, Rachel Yuan, Reuter, Hendrik, ...
DNA microarrays serve to monitor a wide range of molecular events, but emerging applications like measurements of weakly expressed genes or of proteins and their interaction patterns will require...
Yuille, Martin, Van Ommen, Gert-Jan, Bréchot, Christian, Cambon-Thomsen, Anne, Dagher, Georges, Landegren, Ulf, ...
Biobanks are well-organized resources comprising biological samples and associated information that are accessible to scientific investigation. Across Europe, millions of samples with related data...
Taussig, Michael J., Stoevesandt, Oda, Bradbury, Andrew R., Cahill, Dolores, Cambillau, Christian, ...
ProteomeBinders is a new European consortium aiming to establish a comprehensive resource of well-characterized affinity reagents, including but not limited to antibodies, for analysis of the human...
Ulf Landegren, Fredrik Dahl, Mats Nilsson, Simon Fredriksson, Johan Banér, Mats Gullberg, ...
Highly specific high-throughput assays will be required to take full advantage of the accumulating information about the macromolecular composition of cells and tissues, in order to characterize...
ProbeMaker: an extensible framework for design of sets of oligonucleotide probes (2005)
Stenberg, Johan, Nilsson, Mats, Landegren, Ulf
Abstract Background Procedures for genetic analyses based on oligonucleotide probes are powerful tools that can allow highly parallel investigations of genetic material. Such procedures require the...
Szemes, Marianna, Bonants, Peter, De Weerdt, Marjanne, Baner, Johan, Landegren, Ulf, Schoen, Cor D.
Padlock probes (PLPs) are long oligonucleotides, whose ends are complementary to adjacent target sequences. Upon hybridization to the target, the two ends are brought into contact, allowing PLP...
Dahl, Fredrik, Gullberg, Mats, Stenberg, Johan, Landegren, Ulf, Nilsson, Mats
We present a method to specifically select large sets of DNA sequences for parallel amplification by PCR using target-specific oligonucleotide constructs, so-called selectors. The selectors are...
PieceMaker: selection of DNA fragments for selector-guided multiplex amplification (2005)
Stenberg, Johan, Dahl, Fredrik, Landegren, Ulf, Nilsson, Mats
We describe PieceMaker, a software tool for the design of applications of selector probes–oligonucleotide probes that direct circularization of target nucleic acid molecules. Such probes can be...
Wedrén, Sara, Lovmar, Lovisa, Humphreys, Keith, Magnusson, Cecilia, Melhus, Håkan, Syvänen, Ann-Christine, ...
Abstract Introduction Oestrogen receptor α, which mediates the effect of oestrogen in target tissues, is genetically polymorphic. Because breast cancer development is dependent on oestrogenic...
Mendel-Hartvig, Maritha, Kumar, Anil, Landegren, Ulf
Branched nucleic acid strands exist as intermediates in certain biological reactions, and bifurcating DNA also presents interesting opportunities in biotechnological applications. We describe here...
Parallel gene analysis with allele-specific padlock probes and tag microarrays (2003)
Banér, Johan, Isaksson, Anders, Waldenström, Erik, Jarvius, Jonas, Landegren, Ulf, Nilsson, Mats
Parallel, highly specific analysis methods are required to take advantage of the extensive information about DNA sequence variation and of expressed sequences. We present a scalable laboratory...
Ulf Landegren, Fredrik Dahl, Mats Nilsson, Simon Fredriksson, Johan Banér, Mats Gullberg, ...
Highly specific high-throughput assays will be required to take full advantage of the accumulating information about the macromolecular composition of cells and tissues, in order to characterize...
Lindblom, Per, Gerhardt, Holger, Liebner, Stefan, Abramsson, Alexandra, Enge, Maria, Hellström, Mats, ...
Several platelet-derived growth factor (PDGF) and vascular endothelial growth factor (VEGF) family members display C-terminal protein motifs that confer retention of the secreted factors within the...
RNA-templated DNA ligation for transcript analysis (2001)
Nilsson, Mats, Antson, Dan-Oscar, Barbany, Gisela, Landegren, Ulf
Ligase-mediated gene detection has proven valuable for detection and precise distinction of DNA sequence variants. We have recently shown that T4 DNA ligase can also be used to distinguish single...
Expression profiling across many samples via manifold-assisted mRNA processing (2000)
Hagberg, Anette, Barbany, Gisela, Krook, Henrik, Samiotaki, Martina, Landegren, Ulf
Analysis of mRNA provides a condensed view of gene structure, and quantitative analyses can reveal induction of physiological or pathological gene expression programs. One of the main hurdles for...
Kwiatkowski, Marek, Samiotaki, Martina, Lamminmäki, Urpo, Mukkala, Veli-Matti, Landegren, Ulf
Oligonucleotides labelled with detectable groups are essential tools in gene detection. We describe here the synthesis of pyrimidine deoxynucleotide-building blocks, modified at their C-5 position...
Thesis (doctoral)--University of Uppsala, 1984.
RNA-templated DNA ligation for transcript analysis
Nilsson, Mats, Antson, Dan-Oscar, Barbany, Gisela, Landegren, Ulf
Ligase-mediated gene detection has proven valuable for detection and precise distinction of DNA sequence variants. We have recently shown that T4 DNA ligase can also be used to distinguish single...
Expression profiling across many samples via manifold-assisted mRNA processing
Hagberg, Anette, Barbany, Gisela, Krook, Henrik, Samiotaki, Martina, Landegren, Ulf
Analysis of mRNA provides a condensed view of gene structure, and quantitative analyses can reveal induction of physiological or pathological gene expression programs. One of the main hurdles for...
Endothelial PDGF-B retention is required for proper investment of pericytes in the microvessel wall
Lindblom, Per, Gerhardt, Holger, Liebner, Stefan, Abramsson, Alexandra, Enge, Maria, Hellström, Mats, ...
Several platelet-derived growth factor (PDGF) and vascular endothelial growth factor (VEGF) family members display C-terminal protein motifs that confer retention of the secreted factors within the...
Parallel gene analysis with allele-specific padlock probes and tag microarrays
Banér, Johan, Isaksson, Anders, Waldenström, Erik, Jarvius, Jonas, Landegren, Ulf, Nilsson, Mats
Parallel, highly specific analysis methods are required to take advantage of the extensive information about DNA sequence variation and of expressed sequences. We present a scalable laboratory...
Mendel-Hartvig, Maritha, Kumar, Anil, Landegren, Ulf
Branched nucleic acid strands exist as intermediates in certain biological reactions, and bifurcating DNA also presents interesting opportunities in biotechnological applications. We describe here...
Circle-to-circle amplification for precise and sensitive DNA analysis
Dahl, Fredrik, Banér, Johan, Gullberg, Mats, Mendel-Hartvig, Maritha, Landegren, Ulf, Nilsson, Mats
We present a tightly controlled process for strand-specific amplification of circularized DNA molecules. Tandem repeated complements of DNA circles are generated by rolling-circle replication, and...
Cytokine detection by antibody-based proximity ligation
Gullberg, Mats, Gústafsdóttir, Sigrún M., Schallmeiner, Edith, Jarvius, Jonas, Bjarnegård, Mattias, Betsholtz, Christer, ...
Efficient and precise detection techniques, along with extensive repertoires of specific binding reagents, will be needed to meet the challenges of proteome analyses. The recently established...
Oestrogen receptor α gene haplotype and postmenopausal breast cancer risk: a case control study
Wedrén, Sara, Lovmar, Lovisa, Humphreys, Keith, Magnusson, Cecilia, Melhus, Håkan, Syvänen, Ann-Christine, ...
Szemes, Marianna, Bonants, Peter, De Weerdt, Marjanne, Baner, Johan, Landegren, Ulf, Schoen, Cor D.
Padlock probes (PLPs) are long oligonucleotides, whose ends are complementary to adjacent target sequences. Upon hybridization to the target, the two ends are brought into contact, allowing PLP...
Multiplex amplification enabled by selective circularization of large sets of genomic DNA fragments
Dahl, Fredrik, Gullberg, Mats, Stenberg, Johan, Landegren, Ulf, Nilsson, Mats
We present a method to specifically select large sets of DNA sequences for parallel amplification by PCR using target-specific oligonucleotide constructs, so-called selectors. The selectors are...
PieceMaker: selection of DNA fragments for selector-guided multiplex amplification
Stenberg, Johan, Dahl, Fredrik, Landegren, Ulf, Nilsson, Mats
We describe PieceMaker, a software tool for the design of applications of selector probes–oligonucleotide probes that direct circularization of target nucleic acid molecules. Such probes can be...
RNA-templated DNA ligation for transcript analysis
Nilsson, Mats, Antson, Dan-Oscar, Barbany, Gisela, Landegren, Ulf
Ligase-mediated gene detection has proven valuable for detection and precise distinction of DNA sequence variants. We have recently shown that T4 DNA ligase can also be used to distinguish single...
Expression profiling across many samples via manifold-assisted mRNA processing
Hagberg, Anette, Barbany, Gisela, Krook, Henrik, Samiotaki, Martina, Landegren, Ulf
Analysis of mRNA provides a condensed view of gene structure, and quantitative analyses can reveal induction of physiological or pathological gene expression programs. One of the main hurdles for...
Endothelial PDGF-B retention is required for proper investment of pericytes in the microvessel wall
Lindblom, Per, Gerhardt, Holger, Liebner, Stefan, Abramsson, Alexandra, Enge, Maria, Hellström, Mats, ...
Several platelet-derived growth factor (PDGF) and vascular endothelial growth factor (VEGF) family members display C-terminal protein motifs that confer retention of the secreted factors within the...
Parallel gene analysis with allele-specific padlock probes and tag microarrays
Banér, Johan, Isaksson, Anders, Waldenström, Erik, Jarvius, Jonas, Landegren, Ulf, Nilsson, Mats
Parallel, highly specific analysis methods are required to take advantage of the extensive information about DNA sequence variation and of expressed sequences. We present a scalable laboratory...
Mendel-Hartvig, Maritha, Kumar, Anil, Landegren, Ulf
Branched nucleic acid strands exist as intermediates in certain biological reactions, and bifurcating DNA also presents interesting opportunities in biotechnological applications. We describe here...
Circle-to-circle amplification for precise and sensitive DNA analysis
Dahl, Fredrik, Banér, Johan, Gullberg, Mats, Mendel-Hartvig, Maritha, Landegren, Ulf, Nilsson, Mats
We present a tightly controlled process for strand-specific amplification of circularized DNA molecules. Tandem repeated complements of DNA circles are generated by rolling-circle replication, and...
Cytokine detection by antibody-based proximity ligation
Gullberg, Mats, Gústafsdóttir, Sigrún M., Schallmeiner, Edith, Jarvius, Jonas, Bjarnegård, Mattias, Betsholtz, Christer, ...
Efficient and precise detection techniques, along with extensive repertoires of specific binding reagents, will be needed to meet the challenges of proteome analyses. The recently established...
Oestrogen receptor α gene haplotype and postmenopausal breast cancer risk: a case control study
Wedrén, Sara, Lovmar, Lovisa, Humphreys, Keith, Magnusson, Cecilia, Melhus, Håkan, Syvänen, Ann-Christine, ...
Szemes, Marianna, Bonants, Peter, De Weerdt, Marjanne, Baner, Johan, Landegren, Ulf, Schoen, Cor D.
Padlock probes (PLPs) are long oligonucleotides, whose ends are complementary to adjacent target sequences. Upon hybridization to the target, the two ends are brought into contact, allowing PLP...
Multiplex amplification enabled by selective circularization of large sets of genomic DNA fragments
Dahl, Fredrik, Gullberg, Mats, Stenberg, Johan, Landegren, Ulf, Nilsson, Mats
We present a method to specifically select large sets of DNA sequences for parallel amplification by PCR using target-specific oligonucleotide constructs, so-called selectors. The selectors are...
PieceMaker: selection of DNA fragments for selector-guided multiplex amplification
Stenberg, Johan, Dahl, Fredrik, Landegren, Ulf, Nilsson, Mats
We describe PieceMaker, a software tool for the design of applications of selector probes–oligonucleotide probes that direct circularization of target nucleic acid molecules. Such probes can be...
Abramsson, Alexandra, Kurup, Sindhulakshmi, Busse, Marta, Yamada, Shuhei, Lindblom, Per, Schallmeiner, Edith, ...
During vascular development, endothelial platelet-derived growth factor B (PDGF-B) is critical for pericyte recruitment. Deletion of the conserved C-terminal heparin-binding motif impairs PDGF-BB...
In vitro analysis of DNA–protein interactions by proximity ligation
Gustafsdottir, Sigrun M., Schlingemann, Joerg, Rada-Iglesias, Alvaro, Schallmeiner, Edith, Kamali-Moghaddam, Masood, Wadelius, Claes, ...
Protein-binding DNA sequence elements encode a variety of regulated functions of genomes. Information about such elements is currently in a state of rapid growth, but improved methods are required to...
A dual-tag microarray platform for high-performance nucleic acid and protein analyses
Ericsson, Olle, Jarvius, Jonas, Schallmeiner, Edith, Howell, Mathias, Nong, Rachel Yuan, Reuter, Hendrik, ...
DNA microarrays serve to monitor a wide range of molecular events, but emerging applications like measurements of weakly expressed genes or of proteins and their interaction patterns will require...
Padlock and Proximity Probes for in Situ and Array-Based Analyses: Tools for the Post-Genomic Era
Landegren, Ulf, Dahl, Fredrik, Nilsson, Mats, Fredriksson, Simon, Banér, Johan, Gullberg, Mats, ...
Highly specific high-throughput assays will be required to take full advantage of the accumulating information about the macromolecular composition of cells and tissues, in order to characterize...
Szado, Tania, Vanderheyden, Veerle, Parys, Jan B., De Smedt, Humbert, Rietdorf, Katja, Kotelevets, Larissa, ...
Imbalance of signals that control cell survival and death results in pathologies, including cancer and neurodegeneration. Two pathways that are integral to setting the balance between cell survival...
Gyarmati, Péter, Conze, Tim, Zohari, Siamak, LeBlanc, Neil, Nilsson, Mats, Landegren, Ulf, ...
A subtyping assay for both the hemagglutinin (HA) and neuraminidase (NA) surface antigens of the avian influenza virus (AIV) has been developed. The method uses padlock probe chemistry combined with...