Lauer, Jason L., Shohet, J. Leon, Albrecht, Ralph M., Esnault, Stephane, Malter, James S., Von Andrian, Ulrich H., ...
This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying...
Lauer, Jason L., Shohet, J. Leon, Alameldeen, Ralph M., Pratoomtong, C., Murugesan, R., Esnault, Stephane, ...
This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying...
Frenette, Paul S., Subbarao, Sangeetha, Mazo, Irina B., Von Andrian, Ulrich H., Wagner, Denisa D.
The adhesive mechanisms allowing hematopoietic progenitor cells (HPC) homing to the bone marrow (BM) after BM transplantation are poorly understood. We investigated the role of endothelial selectins...
Shimaoka, Motomu, Lu, Chafen, Palframan, Roger T., Von Andrian, Ulrich H., McCormack, Alison, Takagi, Junichi, ...
The integrin αLβ2 has three different domains in its headpiece that have been suggested to either bind ligand or to regulate ligand binding. One of these, the inserted or I domain, has a fold...
A multistep adhesion cascade for lymphoid progenitor cell homing to the thymus
Scimone, M. Lucila, Aifantis, Iannis, Apostolou, Irina, Von Boehmer, Harald, Von Andrian, Ulrich H.
Homing of bone marrow (BM)-derived progenitors to the thymus is essential for T cell development. We have previously reported that two subpopulations of common lymphoid progenitors, CLP-1 and CLP-2,...
Frenette, Paul S., Subbarao, Sangeetha, Mazo, Irina B., Von Andrian, Ulrich H., Wagner, Denisa D.
The adhesive mechanisms allowing hematopoietic progenitor cells (HPC) homing to the bone marrow (BM) after BM transplantation are poorly understood. We investigated the role of endothelial selectins...
Shimaoka, Motomu, Lu, Chafen, Palframan, Roger T., Von Andrian, Ulrich H., McCormack, Alison, Takagi, Junichi, ...
The integrin αLβ2 has three different domains in its headpiece that have been suggested to either bind ligand or to regulate ligand binding. One of these, the inserted or I domain, has a fold...
A multistep adhesion cascade for lymphoid progenitor cell homing to the thymus
Scimone, M. Lucila, Aifantis, Iannis, Apostolou, Irina, Von Boehmer, Harald, Von Andrian, Ulrich H.
Homing of bone marrow (BM)-derived progenitors to the thymus is essential for T cell development. We have previously reported that two subpopulations of common lymphoid progenitors, CLP-1 and CLP-2,...
Murine CXCL14 Is Dispensable for Dendritic Cell Function and Localization within Peripheral Tissues▿
Meuter, Simone, Schaerli, Patrick, Roos, Regula Stuber, Brandau, Oliver, Bösl, Michael R., Von Andrian, Ulrich H., ...
Dendritic cells (DCs) have long been recognized as key regulators of immune responses. However, the process of their recruitment to peripheral tissues and turnover during homeostasis remains largely...
Halin, Cornelia, Scimone, M. Lucila, Bonasio, Roberto, Gauguet, Jean-Marc, Mempel, Thorsten R., Quackenbush, Elizabeth, ...
Sphingosine-1-phosphate (S1P) and its receptor S1P1 control T-cell egress from thymus and secondary lymphoid organs (SLOs). To further define the role of S1P1 in lymphocyte trafficking, we performed...
Aberrant activation of integrin α4β7 suppresses lymphocyte migration to the gut
Park, Eun Jeong, Mora, J. Rodrigo, Carman, Christopher V., Chen, JianFeng, Sasaki, Yoshiteru, Cheng, Guiying, ...
Integrin adhesion molecules mediate lymphocyte migration and homing to normal and inflamed tissues. While the ligand-binding activity of integrins is known to be modulated by conformational changes,...
Swirski, Filip K., Berger, Cedric R., Figueiredo, Jose-Luiz, Mempel, Thorsten R., Von Andrian, Ulrich H., Pittet, Mikael J., ...
The complexity of the tumor microenvironment necessitates that cell behavior is studied in a broad, multi-scale context. Although tomographic and microscopy-based far and near infrared fluorescence...
Random migration precedes stable target cell interactions of tumor-infiltrating T cells
Mrass, Paulus, Takano, Hajime, Ng, Lai Guan, Daxini, Sachin, Lasaro, Marcio O., Iparraguirre, Amaya, ...
The tumor microenvironment is composed of an intricate mixture of tumor and host-derived cells that engage in a continuous interplay. T cells are particularly important in this context as they may...
CCR7 ligands stimulate the intranodal motility of T lymphocytes in vivo
Worbs, Tim, Mempel, Thorsten R., Bölter, Jasmin, Von Andrian, Ulrich H., Förster, Reinhold
In contrast to lymphocyte homing, little is known about molecular cues controlling the motility of lymphocytes within lymphoid organs. Applying intravital two-photon microscopy, we demonstrate that...
Nombela-Arrieta, César, Mempel, Thorsten R., Soriano, Silvia F., Mazo, Irina, Wymann, Matthias P., Hirsch, Emilio, ...
Recent observations using multiphoton intravital microscopy (MP-IVM) have uncovered an unexpectedly high lymphocyte motility within peripheral lymph nodes (PLNs). Lymphocyte-expressed intracellular...
Specific and covalent labeling of a membrane protein with organic fluorochromes and quantum dots
Bonasio, Roberto, Carman, Christopher V., Kim, Enoch, Sage, Peter T., Love, Kerry R., Mempel, Thorsten R., ...
The real-time observation of protein dynamics in living cells and organisms is of fundamental importance for understanding biological processes. Most approaches to labeling proteins exploit...
Scimone, M. Lucila, Felbinger, Thomas W., Mazo, Irina B., Stein, Jens V., Von Andrian, Ulrich H., Weninger, Wolfgang
Central memory CD8+ T cells (TCM) confer superior protective immunity against infections compared with other T cell subsets. TCM recirculate mainly through secondary lymphoid organs, including...
Mora, J. Rodrigo, Cheng, Guiying, Picarella, Dominic, Briskin, Michael, Buchanan, Natasha, Von Andrian, Ulrich H.
T cell activation by intestinal dendritic cells (DC) induces gut-tropism. We show that, reciprocally, DC from peripheral lymph nodes (PLN-DC) induce homing receptors promoting CD8 T cell accumulation...
Stokol, Tracy, O'Donnell, Peter, Xiao, Ling, Knight, Sara, Stavrakis, George, Botto, Marina, ...
Inflammation induced by circulating immunoglobulin G–immune complexes (ICs) characterizes many immune-mediated diseases. In this work, the molecular requirements for the deposition of circulating...
Tenno, Mari, Ohtsubo, Kazuaki, Hagen, Fred K., Ditto, David, Zarbock, Alexander, Schaerli, Patrick, ...
Core-type protein O glycosylation is initiated by polypeptide N-acetylgalactosamine (GalNAc) transferase (ppGalNAcT) activity and produces the covalent linkage of serine and threonine residues of...
CD4 Effector T Cell Subsets in the Response to Influenza: Heterogeneity, Migration, and Function
Román, Eulogia, Miller, Ellen, Harmsen, Allen, Wiley, James, Von Andrian, Ulrich H., Huston, Gail, ...
The immune response of naive CD4 T cells to influenza virus is initiated in the draining lymph nodes and spleen, and only after effectors are generated do antigen-specific cells migrate to the lung...
M'Rini, Christine, Cheng, Guiying, Schweitzer, Colleen, Cavanagh, Lois L., Palframan, Roger T., Mempel, Thorsten R., ...
Lymphocytes home to peripheral lymph nodes (PLNs) via high endothelial venules (HEVs) in the subcortex and incrementally larger collecting venules in the medulla. HEVs express ligands for L-selectin,...
Lymphocyte–HEV Interactions in Lymph Nodes of a Sulfotransferase-deficient Mouse
Van Zante, Annemieke, Gauguet, Jean-Marc, Bistrup, Annette, Tsay, Durwin, Von Andrian, Ulrich H., Rosen, Steven D.
The interaction of L-selectin expressed on lymphocytes with sulfated sialomucin ligands such as CD34 and GlyCAM-1 on high endothelial venules (HEV) of lymph nodes results in lymphocyte rolling and is...
Stein, Jens V., Cheng, Guiying, Stockton, Britt M., Fors, Brian P., Butcher, Eugene C., Von Andrian, Ulrich H.
Adhesion receptors that are known to initiate contact (tethering) between blood-borne leukocytes and their endothelial counterreceptors are frequently concentrated on the microvilli of leukocytes....
Interaction of Dendritic Cells with Skin Endothelium: A New Perspective on Immunosurveillance
Robert, Caroline, Fuhlbrigge, Robert C., Kieffer, J. David, Ayehunie, Seyoum, Hynes, Richard O., Cheng, Guiying, ...
The goal of this study was to determine the mechanisms by which dendritic cells (DCs) in blood could interact with endothelium, a prerequisite to extravasation into tissues. Our results indicate that...
Tager, Andrew M., Dufour, Jennifer H., Goodarzi, Katayoon, Bercury, Scott D., Von Andrian, Ulrich H., Luster, Andrew D.
Leukotriene B4 (LTB4) is a potent chemoattractant active on multiple leukocytes, including neutrophils, macrophages, and eosinophils, and is implicated in the pathogenesis of a variety of...
Baekkevold, Espen S., Yamanaka, Takeshi, Palframan, Roger T., Carlsen, Hege S., Reinholt, Finn P., Von Andrian, Ulrich H., ...
Lymphocyte homing to secondary lymphoid tissue is defined by a multistep sequence of interactions between lymphocytes and endothelial cells in high endothelial venules (HEVs). After initial...
Migratory Properties of Naive, Effector, and Memory Cd8+ T Cells
Weninger, Wolfgang, Crowley, Maura A., Manjunath, N., Von Andrian, Ulrich H.
It has been proposed that two different antigen-experienced T cell subsets may be distinguishable by their preferential ability to home to lymphoid organs (central memory cells) or nonlymphoid...
Stein, Jens V., Rot, Antal, Luo, Yi, Narasimhaswamy, Manjunath, Nakano, Hideki, Gunn, Michael D., ...
T cell homing to peripheral lymph nodes (PLNs) is defined by a multistep sequence of interactions between lymphocytes and endothelial cells in high endothelial venules (HEVs). After initial tethering...
Palframan, Roger T., Jung, Steffen, Cheng, Guiying, Weninger, Wolfgang, Luo, Yi, Dorf, Martin, ...
Interstitial fluid is constantly drained into lymph nodes (LNs) via afferent lymph vessels. This conduit enables monocyte-derived macrophages and dendritic cells to access LNs from peripheral...
Mazo, Irina B., Gutierrez-Ramos, Jose-Carlos, Frenette, Paul S., Hynes, Richard O., Wagner, Denisa D., Von Andrian, Ulrich H.
We have used intravital microscopy to study physiologically perfused microvessels in murine bone marrow (BM). BM sinusoids and venules, but not adjacent bone vessels, supported rolling interactions...
Wolf, Katarina, Mazo, Irina, Leung, Harry, Engelke, Katharina, Von Andrian, Ulrich H., Deryugina, Elena I., ...
Invasive tumor dissemination in vitro and in vivo involves the proteolytic degradation of ECM barriers. This process, however, is only incompletely attenuated by protease inhibitor–based treatment,...
How Antigen Quantity and Quality Determine T-Cell Decisions in Lymphoid Tissue▿ †
Zheng, Huan, Jin, Bo, Henrickson, Sarah E., Perelson, Alan S., Von Andrian, Ulrich H., Chakraborty, Arup K.
T lymphocytes (T cells) express T-cell receptor (TCR) molecules on their surface that can recognize peptides (p) derived from antigenic proteins bound to products of the major histocompatibility...
Dona, Maria, Fredman, Gabrielle, Schwab, Jan M., Chiang, Nan, Arita, Makoto, Goodarzi, Ahmad, ...
Resolvin E1 (RvE1) is an omega-3 eicosapentaenoic acid (EPA)–derived lipid mediator generated during resolution of inflammation and in human vasculature via leukocyte-endothelial cell interactions....