Hidalgo, Aurelio, Schließmann, Anna, Molina, Rafael, Hermoso, Juan, Bornscheuer, Uwe T.
Protein engineering is currently performed either by rational design, focusing in most cases on only a few positions modified by site-directed mutagenesis, or by directed molecular evolution, in...
Heinze, Birgit, Kourist, Robert, Fransson, Linda, Hult, Karl, Bornscheuer, Uwe T.
Enzyme-catalyzed kinetic resolutions of secondary alcohols are a standard procedure today and several lipases and esterases have been described to show high activity and enantioselectivity. In...
Heinze, Birgit, Kourist, Robert, Fransson, Linda, Hult, Karl, Bornscheuer, Uwe T.
Enzyme-catalyzed kinetic resolutions of secondary alcohols are a standard procedure today and several lipases and esterases have been described to show high activity and enantioselectivity. In...
Musidlowska-Persson, Anna, Bornscheuer, Uwe T.
It was shown recently that proline‐β‐naphthylamidase from pig liver resembles the γ‐subunit of pig liver esterase (PLE), which could be functionally expressed in the yeast Pichia...
Heterologous Production of Functional Forms of Rhizopus oryzae Lipase in Escherichia coli
Di Lorenzo, Mirella, Hidalgo, Aurelio, Haas, Michael, Bornscheuer, Uwe T.
To date, expression of the lipase from Rhizopus oryzae (ROL) in Escherichia coli always led to the formation of inclusion bodies and inactive protein. However, the production of active ROL and its...
Heterologous Production of Functional Forms of Rhizopus oryzae Lipase in Escherichia coli
Di Lorenzo, Mirella, Hidalgo, Aurelio, Haas, Michael, Bornscheuer, Uwe T.
To date, expression of the lipase from Rhizopus oryzae (ROL) in Escherichia coli always led to the formation of inclusion bodies and inactive protein. However, the production of active ROL and its...
Di Lorenzo, Mirella, Hidalgo, Aurelio, Molina, Rafael, Hermoso, Juan A., Pirozzi, Domenico, Bornscheuer, Uwe T.
A prolipase from Rhizopus oryzae (proROL) was engineered in order to increase its stability toward lipid oxidation products such as aldehydes with the aim of improving its performance in oleochemical...
Rehdorf, Jessica, Zimmer, Christian L., Bornscheuer, Uwe T.
While the number of available recombinant Baeyer-Villiger monooxygenases (BVMOs) has grown significantly over the last few years, there is still the demand for other BVMOs to expand the biocatalytic...