Uwe Völker

Microbiology (2006), 152, 831–853 DOI 10.1099/mic.0.28530-0 Correspondence (2005)

Uwe Völker

A primary habitat of the Gram-positive bacterium Bacillus subtilis is the upper layer of the soil. Within this ecosystem, B. subtilis experiences a wide variety of environmental challenges and...

Microbiology (2005), 151, 399–420 DOI 10.1099/mic.0.27493-0 Correspondence (2004)

Uwe Völker

During the early stages of endospore development in the bacterium Bacillus subtilis, the rod-shaped cell is partitioned into a small prespore and a much larger mother cell. Each cell type receives a...

Chill induction of the SigB-dependent general stress response in Bacillus subtilis and its contribution to lowtemperature adaptation (2003)

Matthias Brigulla, Tamara Hoffmann, Andrea Krisp, Andrea Völker, Erhard Bremer, Uwe Völker

A variety of environmental and metabolic cues trigger the transient activation of the alternative transcription factor SigB of Bacillus subtilis, which subsequently leads to the induction of more...

Expression of the ςB-Dependent General Stress Regulon Confers Multiple Stress Resistance in Bacillus subtilis

Völker, Uwe, Maul, Björn, Hecker, Michael

The alternative sigma factor ςB of Bacillus subtilis is required for the induction of approximately 100 genes after the imposition of a whole range of stresses and energy limitation. In this study,...

Identification of ςB-Dependent Genes in Bacillus subtilis Using a Promoter Consensus-Directed Search and Oligonucleotide Hybridization

Petersohn, Anja, Bernhardt, Jörg, Gerth, Ulf, Höper, Dirk, Koburger, Torsten, Völker, Uwe, ...

A consensus-directed search for ςB promoters was used to locate potential candidates for new ςB-dependent genes in Bacillus subtilis. Screening of those candidates by oligonucleotide hybridizations...

Mutations in Catabolite Control Protein CcpA Showing Glucose-Independent Regulation in Bacillus megaterium

Küster-Schöck, Elke, Wagner, Andrea, Völker, Uwe, Hillen, Wolfgang

We identified five single amino acid exchanges in CcpA that lead to permanent repression of the xylose utilization genes in the absence of glucose. Other proteins from the CcpA regulon also show...

Forespore-Specific Transcription of the lonB Gene during Sporulation in Bacillus subtilis

Serrano, Monica, Hövel, Sven, Moran, Charles P., Henriques, Adriano O., Völker, Uwe

The Bacillus subtilis genome encodes two members of the Lon family of prokaryotic ATP-dependent proteases. One, LonA, is produced in response to temperature, osmotic, and oxidative stress and has...

Global Analysis of the General Stress Response of Bacillus subtilis

Petersohn, Anja, Brigulla, Matthias, Haas, Stefan, Hoheisel, Jörg D., Völker, Uwe, Hecker, Michael

Gene arrays containing all currently known open reading frames of Bacillus subtilis were used to examine the general stress response of Bacillus. By proteomics, transcriptional analysis, transposon...

Nitrogen Starvation-Induced Chlorosis in Synechococcus PCC 7942. Low-Level Photosynthesis As a Mechanism of Long-Term Survival1

Sauer, Jörg, Schreiber, Ulrich, Schmid, Roland, Völker, Uwe, Forchhammer, Karl

Cells of the non-diazotrophic cyanobacterium Synechococcus sp. strain PCC 7942 acclimate to nitrogen deprivation by differentiating into non-pigmented resting cells, which are able to survive...

Thioredoxin Is an Essential Protein Induced by Multiple Stresses in Bacillus subtilis

Scharf, Christian, Riethdorf, Sabine, Ernst, Henrik, Engelmann, Susanne, Völker, Uwe, Hecker, Michael

Thioredoxin, a small, ubiquitous protein which participates in redox reactions through the reversible oxidation of its active center dithiol to a disulfide, is an essential protein in Bacillus...

Nonnative Proteins Induce Expression of the Bacillus subtilis CIRCE Regulon

Mogk, Axel, Völker, Andrea, Engelmann, Susanne, Hecker, Michael, Schumann, Wolfgang, Völker, Uwe

The chaperone-encoding groESL and dnaK operons constitute the CIRCE regulon of Bacillus subtilis. Both operons are under negative control of the repressor protein HrcA, which interacts with the CIRCE...

One of Two OsmC Homologs in Bacillus subtilis Is Part of the ςB-Dependent General Stress Regulon

Völker, Uwe, Andersen, Kasper Krogh, Antelmann, Haike, Devine, Kevin M., Hecker, Michael

In this report we present the identification and analysis of two Bacillus subtilis genes, yklA and ykzA, which are homologous to the partially RpoS-controlled osmC gene from Escherichia coli. The...

Glycerol-3-Phosphate-Induced Catabolite Repression in Escherichia coli

Eppler, Tanja, Postma, Pieter, Schütz, Alexandra, Völker, Uwe, Boos, Winfried

The formation of glycerol-3-phosphate (G3P) in cells growing on TB causes catabolite repression, as shown by the reduction in malT expression. For this repression to occur, the general proteins of...

High-Salinity-Induced Iron Limitation in Bacillus subtilis

Hoffmann, Tamara, Schütz, Alexandra, Brosius, Margot, Völker, Andrea, Völker, Uwe, Bremer, Erhard

Proteome analysis of Bacillus subtilis cells grown at low and high salinity revealed the induction of 16 protein spots and the repression of 2 protein spots, respectively. Most of these protein spots...

Genomewide Transcriptional Analysis of the Cold Shock Response in Bacillus subtilis

Beckering, Carsten L., Steil, Leif, Weber, Michael H. W., Völker, Uwe, Marahiel, Mohamed A.

Previous studies with two-dimensional gel electrophoresis techniques revealed that the cold shock response in Bacillus subtilis is characterized by rapid induction and accumulation of two classes of...

Chill Induction of the SigB-Dependent General Stress Response in Bacillus subtilis and Its Contribution to Low-Temperature Adaptation

Brigulla, Matthias, Hoffmann, Tamara, Krisp, Andrea, Völker, Andrea, Bremer, Erhard, Völker, Uwe

A variety of environmental and metabolic cues trigger the transient activation of the alternative transcription factor SigB of Bacillus subtilis, which subsequently leads to the induction of more...

Genome-Wide Transcriptional Profiling Analysis of Adaptation of Bacillus subtilis to High Salinity

Steil, Leif, Hoffmann, Tamara, Budde, Ina, Völker, Uwe, Bremer, Erhard

The gram-positive soil bacterium Bacillus subtilis often faces increases in the salinity in its natural habitats. A transcriptional profiling approach was utilized to investigate both the initial...

Assembly of an Oxalate Decarboxylase Produced under σK Control into the Bacillus subtilis Spore Coat

Costa, Teresa, Steil, Leif, Martins, Lígia O., Völker, Uwe, Henriques, Adriano O.

Over 30 polypeptides are synthesized at various times during sporulation in Bacillus subtilis, and they are assembled at the surface of the developing spore to form a multilayer protein structure...

RsbV-Independent Induction of the SigB-Dependent General Stress Regulon of Bacillus subtilis during Growth at High Temperature

Holtmann, Gudrun, Brigulla, Matthias, Steil, Leif, Schütz, Alexandra, Barnekow, Karsta, Völker, Uwe, ...

General stress proteins protect Bacillus subtilis cells against a variety of environmental insults. This adaptive response is particularly important for nongrowing cells, to which it confers a...

Comprehensive Characterization of the Contribution of Individual SigB-Dependent General Stress Genes to Stress Resistance of Bacillus subtilis

Höper, Dirk, Völker, Uwe, Hecker, Michael

The σB-dependent general stress regulon of Bacillus subtilis comprises more than 150 members. Induction of this regulon by imposition of environmental or metabolic stress confers multiple,...

Assembly and Function of a Spore Coat-Associated Transglutaminase of Bacillus subtilis

Zilhão, Rita, Isticato, Rachele, Martins, Lígia O., Steil, Leif, Völker, Uwe, Ricca, Ezio, ...

The assembly of a multiprotein coat around the Bacillus subtilis spore confers resistance to lytic enzymes and noxious chemicals and ensures normal germination. Part of the coat is cross-linked and...

Prognostic Factors Including Proliferation Markers Ki-67, bax, and bcl-2 in Temporal Bone Paraganglioma

Gjuric, Mislav, Völker, Uwe, Katalinic, Alexander, Wolf, Stephan Rüdiger

Valuable criteria with which to predict the clinical behavior of the temporal bone paraganglioma or the response to treatment are lacking. The analysis of markers of cell proliferation is a...

Expression of the ςB-Dependent General Stress Regulon Confers Multiple Stress Resistance in Bacillus subtilis

Völker, Uwe, Maul, Björn, Hecker, Michael

The alternative sigma factor ςB of Bacillus subtilis is required for the induction of approximately 100 genes after the imposition of a whole range of stresses and energy limitation. In this study,...

Identification of ςB-Dependent Genes in Bacillus subtilis Using a Promoter Consensus-Directed Search and Oligonucleotide Hybridization

Petersohn, Anja, Bernhardt, Jörg, Gerth, Ulf, Höper, Dirk, Koburger, Torsten, Völker, Uwe, ...

A consensus-directed search for ςB promoters was used to locate potential candidates for new ςB-dependent genes in Bacillus subtilis. Screening of those candidates by oligonucleotide hybridizations...

Mutations in Catabolite Control Protein CcpA Showing Glucose-Independent Regulation in Bacillus megaterium

Küster-Schöck, Elke, Wagner, Andrea, Völker, Uwe, Hillen, Wolfgang

We identified five single amino acid exchanges in CcpA that lead to permanent repression of the xylose utilization genes in the absence of glucose. Other proteins from the CcpA regulon also show...

Forespore-Specific Transcription of the lonB Gene during Sporulation in Bacillus subtilis

Serrano, Monica, Hövel, Sven, Moran, Charles P., Henriques, Adriano O., Völker, Uwe

The Bacillus subtilis genome encodes two members of the Lon family of prokaryotic ATP-dependent proteases. One, LonA, is produced in response to temperature, osmotic, and oxidative stress and has...

Global Analysis of the General Stress Response of Bacillus subtilis

Petersohn, Anja, Brigulla, Matthias, Haas, Stefan, Hoheisel, Jörg D., Völker, Uwe, Hecker, Michael

Gene arrays containing all currently known open reading frames of Bacillus subtilis were used to examine the general stress response of Bacillus. By proteomics, transcriptional analysis, transposon...

Nitrogen Starvation-Induced Chlorosis in Synechococcus PCC 7942. Low-Level Photosynthesis As a Mechanism of Long-Term Survival1

Sauer, Jörg, Schreiber, Ulrich, Schmid, Roland, Völker, Uwe, Forchhammer, Karl

Cells of the non-diazotrophic cyanobacterium Synechococcus sp. strain PCC 7942 acclimate to nitrogen deprivation by differentiating into non-pigmented resting cells, which are able to survive...

Thioredoxin Is an Essential Protein Induced by Multiple Stresses in Bacillus subtilis

Scharf, Christian, Riethdorf, Sabine, Ernst, Henrik, Engelmann, Susanne, Völker, Uwe, Hecker, Michael

Thioredoxin, a small, ubiquitous protein which participates in redox reactions through the reversible oxidation of its active center dithiol to a disulfide, is an essential protein in Bacillus...

Nonnative Proteins Induce Expression of the Bacillus subtilis CIRCE Regulon

Mogk, Axel, Völker, Andrea, Engelmann, Susanne, Hecker, Michael, Schumann, Wolfgang, Völker, Uwe

The chaperone-encoding groESL and dnaK operons constitute the CIRCE regulon of Bacillus subtilis. Both operons are under negative control of the repressor protein HrcA, which interacts with the CIRCE...

One of Two OsmC Homologs in Bacillus subtilis Is Part of the ςB-Dependent General Stress Regulon

Völker, Uwe, Andersen, Kasper Krogh, Antelmann, Haike, Devine, Kevin M., Hecker, Michael

In this report we present the identification and analysis of two Bacillus subtilis genes, yklA and ykzA, which are homologous to the partially RpoS-controlled osmC gene from Escherichia coli. The...

Glycerol-3-Phosphate-Induced Catabolite Repression in Escherichia coli

Eppler, Tanja, Postma, Pieter, Schütz, Alexandra, Völker, Uwe, Boos, Winfried

The formation of glycerol-3-phosphate (G3P) in cells growing on TB causes catabolite repression, as shown by the reduction in malT expression. For this repression to occur, the general proteins of...

High-Salinity-Induced Iron Limitation in Bacillus subtilis

Hoffmann, Tamara, Schütz, Alexandra, Brosius, Margot, Völker, Andrea, Völker, Uwe, Bremer, Erhard

Proteome analysis of Bacillus subtilis cells grown at low and high salinity revealed the induction of 16 protein spots and the repression of 2 protein spots, respectively. Most of these protein spots...

Genomewide Transcriptional Analysis of the Cold Shock Response in Bacillus subtilis

Beckering, Carsten L., Steil, Leif, Weber, Michael H. W., Völker, Uwe, Marahiel, Mohamed A.

Previous studies with two-dimensional gel electrophoresis techniques revealed that the cold shock response in Bacillus subtilis is characterized by rapid induction and accumulation of two classes of...

Chill Induction of the SigB-Dependent General Stress Response in Bacillus subtilis and Its Contribution to Low-Temperature Adaptation

Brigulla, Matthias, Hoffmann, Tamara, Krisp, Andrea, Völker, Andrea, Bremer, Erhard, Völker, Uwe

A variety of environmental and metabolic cues trigger the transient activation of the alternative transcription factor SigB of Bacillus subtilis, which subsequently leads to the induction of more...

Genome-Wide Transcriptional Profiling Analysis of Adaptation of Bacillus subtilis to High Salinity

Steil, Leif, Hoffmann, Tamara, Budde, Ina, Völker, Uwe, Bremer, Erhard

The gram-positive soil bacterium Bacillus subtilis often faces increases in the salinity in its natural habitats. A transcriptional profiling approach was utilized to investigate both the initial...

Assembly of an Oxalate Decarboxylase Produced under σK Control into the Bacillus subtilis Spore Coat

Costa, Teresa, Steil, Leif, Martins, Lígia O., Völker, Uwe, Henriques, Adriano O.

Over 30 polypeptides are synthesized at various times during sporulation in Bacillus subtilis, and they are assembled at the surface of the developing spore to form a multilayer protein structure...

RsbV-Independent Induction of the SigB-Dependent General Stress Regulon of Bacillus subtilis during Growth at High Temperature

Holtmann, Gudrun, Brigulla, Matthias, Steil, Leif, Schütz, Alexandra, Barnekow, Karsta, Völker, Uwe, ...

General stress proteins protect Bacillus subtilis cells against a variety of environmental insults. This adaptive response is particularly important for nongrowing cells, to which it confers a...

Comprehensive Characterization of the Contribution of Individual SigB-Dependent General Stress Genes to Stress Resistance of Bacillus subtilis

Höper, Dirk, Völker, Uwe, Hecker, Michael

The σB-dependent general stress regulon of Bacillus subtilis comprises more than 150 members. Induction of this regulon by imposition of environmental or metabolic stress confers multiple,...

Assembly and Function of a Spore Coat-Associated Transglutaminase of Bacillus subtilis

Zilhão, Rita, Isticato, Rachele, Martins, Lígia O., Steil, Leif, Völker, Uwe, Ricca, Ezio, ...

The assembly of a multiprotein coat around the Bacillus subtilis spore confers resistance to lytic enzymes and noxious chemicals and ensures normal germination. Part of the coat is cross-linked and...

Prognostic Factors Including Proliferation Markers Ki-67, bax, and bcl-2 in Temporal Bone Paraganglioma

Gjuric, Mislav, Völker, Uwe, Katalinic, Alexander, Wolf, Stephan Rüdiger

Valuable criteria with which to predict the clinical behavior of the temporal bone paraganglioma or the response to treatment are lacking. The analysis of markers of cell proliferation is a...

The Timing of cotE Expression Affects Bacillus subtilis Spore Coat Morphology but Not Lysozyme Resistance▿

Costa, Teresa, Serrano, Mónica, Steil, Leif, Völker, Uwe, Moran, Charles P., Henriques, Adriano O.

The synthesis of structural components and morphogenetic factors required for the assembly of the Bacillus subtilis spore coat is governed by a mother cell-specific transcriptional cascade. The first...

ClpP Modulates the Activity of the Bacillus subtilis Stress Response Transcription Factor, σB▿

Reeves, Adam, Gerth, Ulf, Völker, Uwe, Haldenwang, W. G.

The general stress regulon of Bacillus subtilis is controlled by the activity state of σB, a transcription factor that is switched on following exposure to either physical or nutritional stress....

Meta-Analysis of 28,141 Individuals Identifies Common Variants within Five New Loci That Influence Uric Acid Concentrations

Kolz, Melanie, Johnson, Toby, Sanna, Serena, Teumer, Alexander, Vitart, Veronique, Perola, Markus, ...

Elevated serum uric acid levels cause gout and are a risk factor for cardiovascular disease and diabetes. To investigate the polygenetic basis of serum uric acid levels, we conducted a meta-analysis...