V. A. Bankaitis

Use of mass spectrometry-based lipidomics to probe PITPα (phosphatidylinositol transfer protein α) function inside the nuclei of PITPα+/+ and PITPα-/- cells (2004)

Hunt, A.N., Alb, J.G., Koster, G., Postle, A.D., Bankaitis, V.A.

The mammalian phospholipid exchange protein PITPa (phosphatidylinositol transfer protein alpha), found in both extranuclear and endonuclear compartments, is thought in part to facilitate nuclear...

Mutant rat phosphatidylinositol/phosphatidylcholine transfer proteins specifically defective in phosphatidylinositol transfer: implications for the regulation of phospholipid transfer activity.

Alb, J G, Gedvilaite, A, Cartee, R T, Skinner, H B, Bankaitis, V A

The mammalian phosphatidylinositol/phosphatidylcholine transfer proteins (PI-TPs) catalyze exchange of phosphatidylinositol (PI) or phosphatidylcholine (PC) between membrane bilayers in vitro. We...

The Saccharomyces cerevisiae phosphatidylinositol-transfer protein effects a ligand-dependent inhibition of choline-phosphate cytidylyltransferase activity.

Skinner, H B, McGee, T P, McMaster, C R, Fry, M R, Bell, R M, Bankaitis, V A

The Saccharomyces cerevisiae protein SEC14p is required for Golgi function and cell viability in vivo. This requirement is obviated by mutations that specifically inactivate the CDP-choline pathway...

Functional redundancy of CDP-ethanolamine and CDP-choline pathway enzymes in phospholipid biosynthesis: ethanolamine-dependent effects on steady-state membrane phospholipid composition in Saccharomyces cerevisiae.

McGee, T P, Skinner, H B, Bankaitis, V A

It has been established that yeast membrane phospholipid content is responsive to the inositol and choline content of the growth medium. Alterations in the levels of transcription of phospholipid...

Mutational alterations affecting the export competence of a truncated but fully functional maltose-binding protein signal peptide.

Fikes, J D, Bankaitis, V A, Ryan, J P, Bassford, P J

The wild-type maltose-binding protein (MBP) signal peptide is 26 amino acids in length. A mutationally altered MBP signal peptide has been previously described that is missing one of the basic...

Cloning and characterization of Kluyveromyces lactis SEC14, a gene whose product stimulates Golgi secretory function in Saccharomyces cerevisiae.

Salama, S R, Cleves, A E, Malehorn, D E, Whitters, E A, Bankaitis, V A

The Saccharomyces cerevisiae SEC14 gene encodes a cytosolic factor that is required for secretory protein movement from the Golgi complex. That some conservation of SEC14p function may exist was...

Export and processing of MalE-LacZ hybrid proteins in Escherichia coli.

Rasmussen, B A, Bankaitis, V A, Bassford, P J

Five classes of MalE-LacZ hybrid proteins have previously been characterized. These proteins differ in the amount of the maltose-binding protein (MBP) that is attached to beta-galactosidase. Although...

Proper interaction between at least two components is required for efficient export of proteins to the Escherichia coli cell envelope.

Bankaitis, V A, Bassford, P J

An Escherichia coli mutant carrying delta malE12-18, a 21-base pair deletion confined to the coding DNA of the maltose-binding protein signal peptide, is unable to export maltose-binding protein to...

Cyclic adenosine 3',5'-monophosphate-mediated hyperinduction of araBAD and lacZYA expression in a crp mutant of Escherichia coli K-12.

Bankaitis, V A, Kline, E L

A spontaneous lac+ revertant of an adenylate cyclase deletion strain of Escherichia coli K-12 was isolated and characterized. This revertant, designated strain KC20, exhibited a pleiotropic...

Regulation of adenylate cyclase synthesis in Escherichia coli: studies with cya-lac operon and protein fusion strains.

Bankaitis, V A, Bassford, P J

We have isolated cya-lac operon and protein fusions in Escherichia coli K-12, and we used these to study the regulation of cya, the structural gene for adenylate cyclase. Data obtained from these...

Identification and preliminary characterization of Treponema pallidum protein antigens expressed in Escherichia coli.

Stamm, L V, Kerner, T C, Bankaitis, V A, Bassford, P J

We have previously described the construction in Escherichia coli K-12 of a hybrid plasmid colony bank of Treponema pallidum (Nichols strain) genomic DNA. By screening a portion of this bank with an...

Metabolite gene regulation: imidazole and imidazole derivatives which circumvent cyclic adenosine 3',5'-monophosphate in induction of the Escherichia coli L-arabinose operon.

Kline, E L, Bankaitis, V A, Brown, C S, Montefiori, D C

Imidazole, histidine, histamine, histidinol phosphate, urocanic acid, or imidazolepropionic acid were shown to induce the L-arabinose operon in the absence of cyclic adenosine 3',5'-monophosphate....

Isolation of yeast mutants defective in protein targeting to the vacuole.

Bankaitis, V A, Johnson, L M, Emr, S D

We have constructed a PRC1-SUC2 gene fusion that directs the synthesis in Saccharomyces cerevisiae of a hybrid polypeptide consisting of a 433-residue amino-terminal domain derived from the yeast...

Phospholipid transfer activity is relevant to but not sufficient for the essential function of the yeast SEC14 gene product.

Skinner, H B, Alb, J G, Whitters, E A, Helmkamp, G M, Bankaitis, V A

To investigate several key aspects of phosphatidylinositol transfer protein (PI-TP) function in eukaryotic cells, rat PI-TP was expressed in yeast strains carrying lesions in SEC14, the structural...

Kes1p shares homology with human oxysterol binding protein and participates in a novel regulatory pathway for yeast Golgi-derived transport vesicle biogenesis.

Fang, M, Kearns, B G, Gedvilaite, A, Kagiwada, S, Kearns, M, Fung, M K, ...

The yeast phosphatidylinositol transfer protein (Sec14p) is required for biogenesis of Golgi-derived transport vesicles and cell viability, and this essential Sec14p requirement is abrogated by...

Novel developmentally regulated phosphoinositide binding proteins from soybean whose expression bypasses the requirement for an essential phosphatidylinositol transfer protein in yeast.

Kearns, M A, Monks, D E, Fang, M, Rivas, M P, Courtney, P D, Chen, J, ...

Phosphatidylinositol transfer proteins (PITPs) have been shown to play important roles in regulating a number of signal transduction pathways that couple to vesicle trafficking reactions,...

Sac1p plays a crucial role in microsomal ATP transport, which is distinct from its function in Golgi phospholipid metabolism.

Kochendörfer, K U, Then, A R, Kearns, B G, Bankaitis, V A, Mayinger, P

Analysis of microsomal ATP transport in yeast resulted in the identification of Sac1p as an important factor in efficient ATP uptake into the endoplasmic reticulum (ER) lumen. Yet it remained unclear...

The Yeast Bsd2-1 Mutation Influences Both the Requirement for Phosphatidylinositol Transfer Protein Function and Derepression of Phospholipid Biosynthetic Gene Expression in Yeast

Kagiwada, S., Kearns, B. G., McGee, T. P., Fang, M., Hosaka, K., Bankaitis, V. A.

The BSD2-1 allele renders Saccharomyces cerevisiae independent of its normally essential requirement for phosphatidylinositol transfer protein (Sec14p) in the stimulation of Golgi secretory function...

Mutant rat phosphatidylinositol/phosphatidylcholine transfer proteins specifically defective in phosphatidylinositol transfer: implications for the regulation of phospholipid transfer activity.

Alb, J G, Gedvilaite, A, Cartee, R T, Skinner, H B, Bankaitis, V A

The mammalian phosphatidylinositol/phosphatidylcholine transfer proteins (PI-TPs) catalyze exchange of phosphatidylinositol (PI) or phosphatidylcholine (PC) between membrane bilayers in vitro. We...

The Saccharomyces cerevisiae phosphatidylinositol-transfer protein effects a ligand-dependent inhibition of choline-phosphate cytidylyltransferase activity.

Skinner, H B, McGee, T P, McMaster, C R, Fry, M R, Bell, R M, Bankaitis, V A

The Saccharomyces cerevisiae protein SEC14p is required for Golgi function and cell viability in vivo. This requirement is obviated by mutations that specifically inactivate the CDP-choline pathway...

Functional redundancy of CDP-ethanolamine and CDP-choline pathway enzymes in phospholipid biosynthesis: ethanolamine-dependent effects on steady-state membrane phospholipid composition in Saccharomyces cerevisiae.

McGee, T P, Skinner, H B, Bankaitis, V A

It has been established that yeast membrane phospholipid content is responsive to the inositol and choline content of the growth medium. Alterations in the levels of transcription of phospholipid...

Mutational alterations affecting the export competence of a truncated but fully functional maltose-binding protein signal peptide.

Fikes, J D, Bankaitis, V A, Ryan, J P, Bassford, P J

The wild-type maltose-binding protein (MBP) signal peptide is 26 amino acids in length. A mutationally altered MBP signal peptide has been previously described that is missing one of the basic...

Cloning and characterization of Kluyveromyces lactis SEC14, a gene whose product stimulates Golgi secretory function in Saccharomyces cerevisiae.

Salama, S R, Cleves, A E, Malehorn, D E, Whitters, E A, Bankaitis, V A

The Saccharomyces cerevisiae SEC14 gene encodes a cytosolic factor that is required for secretory protein movement from the Golgi complex. That some conservation of SEC14p function may exist was...

Export and processing of MalE-LacZ hybrid proteins in Escherichia coli.

Rasmussen, B A, Bankaitis, V A, Bassford, P J

Five classes of MalE-LacZ hybrid proteins have previously been characterized. These proteins differ in the amount of the maltose-binding protein (MBP) that is attached to beta-galactosidase. Although...

Proper interaction between at least two components is required for efficient export of proteins to the Escherichia coli cell envelope.

Bankaitis, V A, Bassford, P J

An Escherichia coli mutant carrying delta malE12-18, a 21-base pair deletion confined to the coding DNA of the maltose-binding protein signal peptide, is unable to export maltose-binding protein to...

Cyclic adenosine 3',5'-monophosphate-mediated hyperinduction of araBAD and lacZYA expression in a crp mutant of Escherichia coli K-12.

Bankaitis, V A, Kline, E L

A spontaneous lac+ revertant of an adenylate cyclase deletion strain of Escherichia coli K-12 was isolated and characterized. This revertant, designated strain KC20, exhibited a pleiotropic...

Regulation of adenylate cyclase synthesis in Escherichia coli: studies with cya-lac operon and protein fusion strains.

Bankaitis, V A, Bassford, P J

We have isolated cya-lac operon and protein fusions in Escherichia coli K-12, and we used these to study the regulation of cya, the structural gene for adenylate cyclase. Data obtained from these...

Identification and preliminary characterization of Treponema pallidum protein antigens expressed in Escherichia coli.

Stamm, L V, Kerner, T C, Bankaitis, V A, Bassford, P J

We have previously described the construction in Escherichia coli K-12 of a hybrid plasmid colony bank of Treponema pallidum (Nichols strain) genomic DNA. By screening a portion of this bank with an...

Metabolite gene regulation: imidazole and imidazole derivatives which circumvent cyclic adenosine 3',5'-monophosphate in induction of the Escherichia coli L-arabinose operon.

Kline, E L, Bankaitis, V A, Brown, C S, Montefiori, D C

Imidazole, histidine, histamine, histidinol phosphate, urocanic acid, or imidazolepropionic acid were shown to induce the L-arabinose operon in the absence of cyclic adenosine 3',5'-monophosphate....

Isolation of yeast mutants defective in protein targeting to the vacuole.

Bankaitis, V A, Johnson, L M, Emr, S D

We have constructed a PRC1-SUC2 gene fusion that directs the synthesis in Saccharomyces cerevisiae of a hybrid polypeptide consisting of a 433-residue amino-terminal domain derived from the yeast...

Phospholipid transfer activity is relevant to but not sufficient for the essential function of the yeast SEC14 gene product.

Skinner, H B, Alb, J G, Whitters, E A, Helmkamp, G M, Bankaitis, V A

To investigate several key aspects of phosphatidylinositol transfer protein (PI-TP) function in eukaryotic cells, rat PI-TP was expressed in yeast strains carrying lesions in SEC14, the structural...

Kes1p shares homology with human oxysterol binding protein and participates in a novel regulatory pathway for yeast Golgi-derived transport vesicle biogenesis.

Fang, M, Kearns, B G, Gedvilaite, A, Kagiwada, S, Kearns, M, Fung, M K, ...

The yeast phosphatidylinositol transfer protein (Sec14p) is required for biogenesis of Golgi-derived transport vesicles and cell viability, and this essential Sec14p requirement is abrogated by...

Novel developmentally regulated phosphoinositide binding proteins from soybean whose expression bypasses the requirement for an essential phosphatidylinositol transfer protein in yeast.

Kearns, M A, Monks, D E, Fang, M, Rivas, M P, Courtney, P D, Chen, J, ...

Phosphatidylinositol transfer proteins (PITPs) have been shown to play important roles in regulating a number of signal transduction pathways that couple to vesicle trafficking reactions,...

Sac1p plays a crucial role in microsomal ATP transport, which is distinct from its function in Golgi phospholipid metabolism.

Kochendörfer, K U, Then, A R, Kearns, B G, Bankaitis, V A, Mayinger, P

Analysis of microsomal ATP transport in yeast resulted in the identification of Sac1p as an important factor in efficient ATP uptake into the endoplasmic reticulum (ER) lumen. Yet it remained unclear...

The Yeast Bsd2-1 Mutation Influences Both the Requirement for Phosphatidylinositol Transfer Protein Function and Derepression of Phospholipid Biosynthetic Gene Expression in Yeast

Kagiwada, S., Kearns, B. G., McGee, T. P., Fang, M., Hosaka, K., Bankaitis, V. A.

The BSD2-1 allele renders Saccharomyces cerevisiae independent of its normally essential requirement for phosphatidylinositol transfer protein (Sec14p) in the stimulation of Golgi secretory function...