V. C. Culotta

The ATX1 gene of Saccharomyces cerevisiae encodes a small metal homeostasis factor that protects cells against reactive oxygen toxicity.

Lin, S J, Culotta, V C

In aerobic organisms, protection against oxidative damage involves the combined action of highly specialized antioxidant enzymes, such as superoxide dismutase (SOD) and catalase. Here we describe the...

Mutations in PMR1 suppress oxidative damage in yeast cells lacking superoxide dismutase.

Lapinskas, P J, Cunningham, K W, Liu, X F, Fink, G R, Culotta, V C

Mutants of Saccharomyces cerevisiae lacking a functional SOD1 gene encoding Cu/Zn superoxide dismutase (SOD) are sensitive to atmospheric levels of oxygen and are auxotrophic for lysine and...

Suppression of oxidative damage by Saccharomyces cerevisiae ATX2, which encodes a manganese-trafficking protein that localizes to Golgi-like vesicles.

Lin, S J, Culotta, V C

Oxygen toxicity in Saccharomyces cerevisiae lacking the copper/zinc superoxide dismutase (SOD1) can be suppressed by overexpression of the S. cerevisiae ATX2 gene. Multiple copies of ATX2 were found...

Copper and the ACE1 regulatory protein reversibly induce yeast metallothionein gene transcription in a mouse extract.

Culotta, V C, Hsu, T, Hu, S, Fürst, P, Hamer, D

We describe a cell-free system in which the transcription of the yeast metallothionein gene is inducible by the addition of metal ions plus a specific regulatory protein. Efficient transcription...

Mouse and frog violate the paradigm of species-specific transcription of ribosomal RNA genes.

Culotta, V C, Wilkinson, J K, Sollner-Webb, B

Transcription of ribosomal RNA genes by RNA polymerase I is generally accepted as being highly species specific, a conclusion based on numerous reports that rRNA genes of one species are not...

Fine mapping of a mouse metallothionein gene metal response element.

Culotta, V C, Hamer, D H

Metal-regulated transcription of metallothionein (MT) genes in higher eucaryotes involves multiple copies of a highly conserved 17-base-pair metal-regulatory element (MRE). We have assayed by...

Transcription factor MBF-I interacts with metal regulatory elements of higher eucaryotic metallothionein genes.

Imbert, J, Zafarullah, M, Culotta, V C, Gedamu, L, Hamer, D

Metallothionein (MT) gene promoters in higher eucaryotes contain multiple metal regulatory elements (MREs) that are responsible for the metal induction of MT gene transcription. We identified and...

Factors and nucleotide sequences that direct ribosomal DNA transcription and their relationship to the stable transcription complex.

Tower, J, Culotta, V C, Sollner-Webb, B

We have studied the protein components and nucleic acid sequences involved in stably activating the ribosomal DNA (rDNA) template and in directing accurate transcription of mammalian rRNA genes. Two...

Eucaryotic transcription complexes are specifically associated in large sedimentable structures: rapid isolation of polymerase I, II, and III transcription factors.

Culotta, V C, Wides, R J, Sollner-Webb, B

RNA synthesis in eucaryotes takes place on template molecules that are activated by stably associating with limiting transcription factors. In this paper we demonstrate that such stable transcription...

The requirement for yeast superoxide dismutase is bypassed through mutations in BSD2, a novel metal homeostasis gene.

Liu, X F, Culotta, V C

Oxygen toxicity in Saccharomyces cerevisiae strains lacking superoxide dismutase can be suppressed through mutations in either the BSD1 or BSD2 gene. In this report, we demonstrate that the BSD2 gene...

The ATX1 gene of Saccharomyces cerevisiae encodes a small metal homeostasis factor that protects cells against reactive oxygen toxicity.

Lin, S J, Culotta, V C

In aerobic organisms, protection against oxidative damage involves the combined action of highly specialized antioxidant enzymes, such as superoxide dismutase (SOD) and catalase. Here we describe the...

Mutations in PMR1 suppress oxidative damage in yeast cells lacking superoxide dismutase.

Lapinskas, P J, Cunningham, K W, Liu, X F, Fink, G R, Culotta, V C

Mutants of Saccharomyces cerevisiae lacking a functional SOD1 gene encoding Cu/Zn superoxide dismutase (SOD) are sensitive to atmospheric levels of oxygen and are auxotrophic for lysine and...

Suppression of oxidative damage by Saccharomyces cerevisiae ATX2, which encodes a manganese-trafficking protein that localizes to Golgi-like vesicles.

Lin, S J, Culotta, V C

Oxygen toxicity in Saccharomyces cerevisiae lacking the copper/zinc superoxide dismutase (SOD1) can be suppressed by overexpression of the S. cerevisiae ATX2 gene. Multiple copies of ATX2 were found...

Copper and the ACE1 regulatory protein reversibly induce yeast metallothionein gene transcription in a mouse extract.

Culotta, V C, Hsu, T, Hu, S, Fürst, P, Hamer, D

We describe a cell-free system in which the transcription of the yeast metallothionein gene is inducible by the addition of metal ions plus a specific regulatory protein. Efficient transcription...

Mouse and frog violate the paradigm of species-specific transcription of ribosomal RNA genes.

Culotta, V C, Wilkinson, J K, Sollner-Webb, B

Transcription of ribosomal RNA genes by RNA polymerase I is generally accepted as being highly species specific, a conclusion based on numerous reports that rRNA genes of one species are not...

The requirement for yeast superoxide dismutase is bypassed through mutations in BSD2, a novel metal homeostasis gene.

Liu, X F, Culotta, V C

Oxygen toxicity in Saccharomyces cerevisiae strains lacking superoxide dismutase can be suppressed through mutations in either the BSD1 or BSD2 gene. In this report, we demonstrate that the BSD2 gene...

Fine mapping of a mouse metallothionein gene metal response element.

Culotta, V C, Hamer, D H

Metal-regulated transcription of metallothionein (MT) genes in higher eucaryotes involves multiple copies of a highly conserved 17-base-pair metal-regulatory element (MRE). We have assayed by...

Transcription factor MBF-I interacts with metal regulatory elements of higher eucaryotic metallothionein genes.

Imbert, J, Zafarullah, M, Culotta, V C, Gedamu, L, Hamer, D

Metallothionein (MT) gene promoters in higher eucaryotes contain multiple metal regulatory elements (MREs) that are responsible for the metal induction of MT gene transcription. We identified and...

Factors and nucleotide sequences that direct ribosomal DNA transcription and their relationship to the stable transcription complex.

Tower, J, Culotta, V C, Sollner-Webb, B

We have studied the protein components and nucleic acid sequences involved in stably activating the ribosomal DNA (rDNA) template and in directing accurate transcription of mammalian rRNA genes. Two...

Eucaryotic transcription complexes are specifically associated in large sedimentable structures: rapid isolation of polymerase I, II, and III transcription factors.

Culotta, V C, Wides, R J, Sollner-Webb, B

RNA synthesis in eucaryotes takes place on template molecules that are activated by stably associating with limiting transcription factors. In this paper we demonstrate that such stable transcription...