Campellone, Kenneth Geno, Cheng, Hui-Chun, Robbins, Douglas, Siripala, Anosha D., McGhie, Emma J., Hayward, Richard D., ...
Enterohemorrhagic Escherichia coli (EHEC) generate F-actin-rich adhesion pedestals by delivering effector proteins into mammalian cells. These effectors include the translocated receptor Tir, along...
Flexibility in a Drug Transport Accessory Protein: Molecular Dynamics Simulations of MexA (2008)
Loredana Vaccaro, Vassilis Koronakis
ABSTRACT Drug resistance in Gram-negative bacteria may be conferred via efflux through a tripartite complex of an inner membrane pump, an outer membrane pore, and a periplasmic adaptor protein. These...
Exploiting pathogenic Escherichia coli to model transmembrane receptor signalling (2006)
Hayward, Richard D., Leong, John M., Koronakis, Vassilis, Campellone, Kenneth Geno
Many microbial pathogens manipulate the actin cytoskeleton of eukaryotic target cells to promote their internalization, intracellular motility and dissemination. Enteropathogenic and...
Koronakis, Vassilis, Cross, Michael, Hughes, Colin
The genes hly A and hly B dictating synthesis and secretion of hemolytic toxin by Escherichia coli are transcribed as part of an operon hly C,hly A,hly B but are separated by an inverted repeat and...
Hyland, Caroline, Vuillard, Laurent, Hughes, Colin, Koronakis, Vassilis
The 1,024-amino-acid acylated hemolysin of Escherichia coli subverts host cell functions and causes cell lysis. Both activities require insertion of the toxin into target mammalian cell membranes. To...
Stanley, Peter, Koronakis, Vassilis, Hughes, Colin
The pore-forming hemolysin (HlyA) of Escherichia coli represents a unique class of bacterial toxins that require a posttranslational modification for activity. The inactive protoxin pro-HlyA is...
Transition to the open state of the TolC periplasmic tunnel entrance
Andersen, Christian, Koronakis, Eva, Bokma, Evert, Eswaran, Jeyanthy, Humphreys, Daniel, Hughes, Colin, ...
The TolC channel-tunnel spans the bacterial outer membrane and periplasm, providing a large exit duct for protein export and multidrug efflux when recruited by substrate-engaged inner membrane...
McGhie, Emma J., Hayward, Richard D., Koronakis, Vassilis
Pathogen-induced remodelling of the host cell actin cytoskeleton drives internalization of invasive Salmon ella by non-phagocytic intestinal epithelial cells. Two Salmonella actin-binding proteins...
Structure of the periplasmic component of a bacterial drug efflux pump
Higgins, Matthew K., Bokma, Evert, Koronakis, Eva, Hughes, Colin, Koronakis, Vassilis
Multidrug resistance among Gram-negative bacteria is conferred by three-component membrane pumps that expel diverse antibiotics from the cell. These efflux pumps consist of an inner membrane...
Chunnel vision: Export and efflux through bacterial channel-tunnels
Andersen, Christian, Hughes, Colin, Koronakis, Vassilis
The Escherichia coli TolC protein is central to toxin export and drug efflux across the inner and outer cell membranes and the intervening periplasmic space. The crystal structure has revealed that...
Hyland, Caroline, Vuillard, Laurent, Hughes, Colin, Koronakis, Vassilis
The 1,024-amino-acid acylated hemolysin of Escherichia coli subverts host cell functions and causes cell lysis. Both activities require insertion of the toxin into target mammalian cell membranes. To...
Stanley, Peter, Koronakis, Vassilis, Hughes, Colin
The pore-forming hemolysin (HlyA) of Escherichia coli represents a unique class of bacterial toxins that require a posttranslational modification for activity. The inactive protoxin pro-HlyA is...
Transition to the open state of the TolC periplasmic tunnel entrance
Andersen, Christian, Koronakis, Eva, Bokma, Evert, Eswaran, Jeyanthy, Humphreys, Daniel, Hughes, Colin, ...
The TolC channel-tunnel spans the bacterial outer membrane and periplasm, providing a large exit duct for protein export and multidrug efflux when recruited by substrate-engaged inner membrane...
McGhie, Emma J., Hayward, Richard D., Koronakis, Vassilis
Pathogen-induced remodelling of the host cell actin cytoskeleton drives internalization of invasive Salmon ella by non-phagocytic intestinal epithelial cells. Two Salmonella actin-binding proteins...
Structure of the periplasmic component of a bacterial drug efflux pump
Higgins, Matthew K., Bokma, Evert, Koronakis, Eva, Hughes, Colin, Koronakis, Vassilis
Multidrug resistance among Gram-negative bacteria is conferred by three-component membrane pumps that expel diverse antibiotics from the cell. These efflux pumps consist of an inner membrane...
Chunnel vision: Export and efflux through bacterial channel-tunnels
Andersen, Christian, Hughes, Colin, Koronakis, Vassilis
The Escherichia coli TolC protein is central to toxin export and drug efflux across the inner and outer cell membranes and the intervening periplasmic space. The crystal structure has revealed that...
Brawn, Lyndsey C., Hayward, Richard D., Koronakis, Vassilis
Salmonellae employ two type III secretion systems (T3SSs), SPI1 and SPI2, to deliver virulence effectors into mammalian cells. SPI1 effectors, including actin-binding SipA, trigger initial bacterial...
Flexibility in a Drug Transport Accessory Protein: Molecular Dynamics Simulations of MexA
Vaccaro, Loredana, Koronakis, Vassilis, Sansom, Mark S. P.
Drug resistance in Gram-negative bacteria may be conferred via efflux through a tripartite complex of an inner membrane pump, an outer membrane pore, and a periplasmic adaptor protein. These are...
Lobedanz, Sune, Bokma, Evert, Symmons, Martyn F., Koronakis, Eva, Hughes, Colin, Koronakis, Vassilis
Bacteria such as Escherichia coli and Pseudomonas aeruginosa expel antibiotics and other inhibitors via tripartite multidrug efflux pumps spanning the inner and outer membranes and the intervening...
Deciphering Interplay between Salmonella Invasion Effectors
Cain, Robert J., Hayward, Richard D., Koronakis, Vassilis
Bacterial pathogens have evolved a specialized type III secretion system (T3SS) to translocate virulence effector proteins directly into eukaryotic target cells. Salmonellae deploy effectors that...
Campellone, Kenneth G., Cheng, Hui-Chun, Robbins, Douglas, Siripala, Anosha D., McGhie, Emma J., Hayward, Richard D., ...
Enterohemorrhagic Escherichia coli (EHEC) generate F-actin–rich adhesion pedestals by delivering effector proteins into mammalian cells. These effectors include the translocated receptor Tir, along...
Salmonella takes control: effector-driven manipulation of the host
McGhie, Emma J, Brawn, Lyndsey C, Hume, Peter J, Humphreys, Daniel, Koronakis, Vassilis
Salmonella pathogenesis relies upon the delivery of over thirty specialised effector proteins into the host cell via two distinct type III secretion systems. These effectors act in concert to subvert...
Humphreys, Daniel, Hume, Peter J., Koronakis, Vassilis
Virulence effectors delivered into intestinal epithelial cells by Salmonella trigger actin remodeling to direct pathogen internalization and intracellular replication in Salmonella-containing...
The assembled structure of a complete tripartite bacterial multidrug efflux pump
Symmons, Martyn F., Bokma, Evert, Koronakis, Eva, Hughes, Colin, Koronakis, Vassilis
Bacteria like Escherichia coli and Pseudomonas aeruginosa expel drugs via tripartite multidrug efflux pumps spanning both inner and outer membranes and the intervening periplasm. In these pumps a...