Vivian Bellofatto

Predicting Consensus Structures for RNA Alignments Via Pseudo-Energy Minimization (2009)

Junilda Spirollari, Kaizhong Zhang, Vivian Bellofatto, Yongkyu Park, Bruce A. Shapiro

Thermodynamic processes with free energy parameters are often used in algorithms that solve the free energy minimization problem to predict secondary structures of single RNA sequences. While results...

Identification of the HIT-45 protein from Trypanosoma brucei as an FHIT protein/dinucleoside triphosphatase: Substrate specificity studies on the recombinant and endogenous proteins (2009)

Banerjee, Hiren, Palenchar, Jennifer B., Lukaszewicz, Maciej, Bojarska, Elzbieta, Stepinski, Janusz, Jemielity, Jacek, ...

A new member of the FHIT protein family, designated HIT-45, has been identified in the African trypanosome Trypanosoma brucei. Recombinant HIT-45 proteins were purified from trypanosomal and...

RADAR: a web server for RNA data analysis and research (2007)

Khaladkar, Mugdha, Bellofatto, Vivian, Wang, Jason T. L., Tian, Bin, Shapiro, Bruce A.

RADAR is a web server that provides a multitude of functionality for RNA data analysis and research. It can align structure-annotated RNA sequences so that both sequence and structure information are...

Identification of novel snRNA-specific Sm proteins that bind selectively to U2 and U4 snRNAs in Trypanosoma brucei (2007)

Tkacz, Itai Dov, Lustig, Yaniv, Stern, Michael Zeev, Biton, Moshe, Salmon-Divon, Mali, Das, Anish, ...

In eukaryotes the seven Sm core proteins bind to U1, U2, U4, and U5 snRNAs. In Trypanosoma brucei, Sm proteins have been implicated in binding both spliced leader (SL) and U snRNAs. In this study, we...

Identification of novel snRNA-specific Sm proteins that bind selectively to U2 and U4 snRNAs in Trypanosoma brucei (2006)

Tkacz, Itai Dov, Lustig, Yaniv, Stern, Michael Zeev, Biton, Moshe, Salmon-Divon, Mali, Das, Anish, ...

In eukaryotes the seven Sm core proteins bind to U1, U2, U4, and U5 snRNAs. In Trypanosoma brucei, Sm proteins have been implicated in binding both spliced leader (SL) and U snRNAs. In this study, we...

Identification of novel snRNA-specific Sm proteins that bind selectively to U2 and U4 snRNAs in Trypanosoma brucei (2006)

Tkacz, Itai Dov, Lustig, Yaniv, Stern, Michael Zeev, Biton, Moshe, Salmon-Divon, Mali, Das, Anish, ...

In eukaryotes the seven Sm core proteins bind to U1, U2, U4, and U5 snRNAs. In Trypanosoma brucei, Sm proteins have been implicated in binding both spliced leader (SL) and U snRNAs. In this study, we...

Characterization of deadenylation in trypanosome extracts and its inhibition by poly(A)-binding protein Pab1p (2004)

MILONE, JOSEPH, WILUSZ, JEFFREY, BELLOFATTO, VIVIAN

The stability of mRNAs is an important point in the regulation of gene expression in eukaryotes. The mRNA turnover pathways have been identified in yeast and mammals. However, mRNA turnover pathways...

Identification of mRNA decapping activities and an ARE-regulated 3' to 5' exonuclease activity in trypanosome extracts (2002)

Milone, Joseph, Wilusz, Jeffrey, Bellofatto, Vivian

mRNA turnover is a regulated process that contributes to the steady state level of cytoplasmic mRNA. The amount of each mRNA determines, to a large extent, the amount of protein produced by that...

Trypanosome spliced leader RNA genes contain the first identified RNA polymerase II gene promoter in these organisms (2001)

Gilinger, Gwen, Bellofatto, Vivian

Typical general transcription factors, such as TATA binding protein and TFII B, have not yet been identified in any member of the Trypanosomatidae family of parasitic protozoa. Interestingly, mRNA...

Differential response to RNA trans-splicing signals within the phosphoglycerate kinase gene cluster in Trypanosoma brucei (1993)

Kapotas, Nikitas, Bellofatto, Vivian

In trypanosomatlds, nuclear pre-mRNA splicing is exclusively a trans-spliclng reaction in which a capped, 39 nt exon, the mini-exon, is positioned 5' to an open reading frame. Differential RNA...

Discontinuous transcription in Leptomonas seymouri: presence of intact and interrupted mini-exon gene families (1988)

Bellofatto, Vivian, Cooper, Robin, Cross, George A.M.

Mature mRNAs of trypanosomatid protozoa result from the joining of at least two exons, which are initially transcribed as separate RNAs. In all trypanosomatids examined to date, the first exon...

Trypanosome spliced leader RNA genes contain the first identified RNA polymerase II gene promoter in these organisms

Gilinger, Gwen, Bellofatto, Vivian

Typical general transcription factors, such as TATA binding protein and TFII B, have not yet been identified in any member of the Trypanosomatidae family of parasitic protozoa. Interestingly, mRNA...

Identification of mRNA decapping activities and an ARE-regulated 3′ to 5′ exonuclease activity in trypanosome extracts

Milone, Joseph, Wilusz, Jeffrey, Bellofatto, Vivian

mRNA turnover is a regulated process that contributes to the steady state level of cytoplasmic mRNA. The amount of each mRNA determines, to a large extent, the amount of protein produced by that...

RNA polymerase II-dependent transcription in trypanosomes is associated with a SNAP complex-like transcription factor

Das, Anish, Bellofatto, Vivian

Spliced leader RNA transcription is essential for cell viability in trypanosomes. The SL RNA genes are expressed from the only defined RNA polymerase II-dependent promoter identified to date in the...

Trypanosomal TBP Functions with the Multisubunit Transcription Factor tSNAP To Direct Spliced-Leader RNA Gene Expression

Das, Anish, Zhang, Qing, Palenchar, Jennifer B., Chatterjee, Bithi, Cross, George A. M., Bellofatto, Vivian

Protein-coding genes of trypanosomes are mainly transcribed polycistronically and cleaved into functional mRNAs in a process that requires trans splicing of a capped 39-nucleotide RNA derived from a...

Characterization of deadenylation in trypanosome extracts and its inhibition by poly(A)-binding protein Pab1p

MILONE, JOSEPH, WILUSZ, JEFFREY, BELLOFATTO, VIVIAN

The stability of mRNAs is an important point in the regulation of gene expression in eukaryotes. The mRNA turnover pathways have been identified in yeast and mammals. However, mRNA turnover pathways...

A Divergent Transcription Factor TFIIB in Trypanosomes Is Required for RNA Polymerase II-Dependent Spliced Leader RNA Transcription and Cell Viability

Palenchar, Jennifer B., Liu, Wenzhe, Palenchar, Peter M., Bellofatto, Vivian

Transcription by RNA polymerase II in trypanosomes deviates from the standard eukaryotic paradigm. Genes are transcribed polycistronically and subsequently cleaved into functional mRNAs, requiring...

Trypanosome spliced leader RNA genes contain the first identified RNA polymerase II gene promoter in these organisms

Gilinger, Gwen, Bellofatto, Vivian

Typical general transcription factors, such as TATA binding protein and TFII B, have not yet been identified in any member of the Trypanosomatidae family of parasitic protozoa. Interestingly, mRNA...

Identification of mRNA decapping activities and an ARE-regulated 3′ to 5′ exonuclease activity in trypanosome extracts

Milone, Joseph, Wilusz, Jeffrey, Bellofatto, Vivian

mRNA turnover is a regulated process that contributes to the steady state level of cytoplasmic mRNA. The amount of each mRNA determines, to a large extent, the amount of protein produced by that...

RNA polymerase II-dependent transcription in trypanosomes is associated with a SNAP complex-like transcription factor

Das, Anish, Bellofatto, Vivian

Spliced leader RNA transcription is essential for cell viability in trypanosomes. The SL RNA genes are expressed from the only defined RNA polymerase II-dependent promoter identified to date in the...

Trypanosomal TBP Functions with the Multisubunit Transcription Factor tSNAP To Direct Spliced-Leader RNA Gene Expression

Das, Anish, Zhang, Qing, Palenchar, Jennifer B., Chatterjee, Bithi, Cross, George A. M., Bellofatto, Vivian

Protein-coding genes of trypanosomes are mainly transcribed polycistronically and cleaved into functional mRNAs in a process that requires trans splicing of a capped 39-nucleotide RNA derived from a...

Characterization of deadenylation in trypanosome extracts and its inhibition by poly(A)-binding protein Pab1p

MILONE, JOSEPH, WILUSZ, JEFFREY, BELLOFATTO, VIVIAN

The stability of mRNAs is an important point in the regulation of gene expression in eukaryotes. The mRNA turnover pathways have been identified in yeast and mammals. However, mRNA turnover pathways...

A Divergent Transcription Factor TFIIB in Trypanosomes Is Required for RNA Polymerase II-Dependent Spliced Leader RNA Transcription and Cell Viability

Palenchar, Jennifer B., Liu, Wenzhe, Palenchar, Peter M., Bellofatto, Vivian

Transcription by RNA polymerase II in trypanosomes deviates from the standard eukaryotic paradigm. Genes are transcribed polycistronically and subsequently cleaved into functional mRNAs, requiring...

Identification of novel snRNA-specific Sm proteins that bind selectively to U2 and U4 snRNAs in Trypanosoma brucei

Tkacz, Itai Dov, Lustig, Yaniv, Stern, Michael Zeev, Biton, Moshe, Salmon-Divon, Mali, Das, Anish, ...

In eukaryotes the seven Sm core proteins bind to U1, U2, U4, and U5 snRNAs. In Trypanosoma brucei, Sm proteins have been implicated in binding both spliced leader (SL) and U snRNAs. In this study, we...

RADAR: a web server for RNA data analysis and research

Khaladkar, Mugdha, Bellofatto, Vivian, Wang, Jason T. L., Tian, Bin, Shapiro, Bruce A.

RADAR is a web server that provides a multitude of functionality for RNA data analysis and research. It can align structure-annotated RNA sequences so that both sequence and structure information are...

Spliced-leader RNA silencing: a novel stress-induced mechanism in Trypanosoma brucei

Lustig, Yaniv, Sheiner, Lilach, Vagima, Yaron, Goldshmidt, Hanoch, Das, Anish, Bellofatto, Vivian, ...

The signal-recognition particle (SRP) mediates the translocation of membrane and secretory proteins across the endoplasmic reticulum upon interaction with the SRP receptor. In trypanosomes, the main...

The Non-Canonical CTD of RNAP-II Is Essential for Productive RNA Synthesis in Trypanosoma brucei

Das, Anish, Bellofatto, Vivian

The carboxy-terminal domain (CTD) of the largest subunit (RPB1) of RNA polymerase II (RNAP-II) is essential for gene expression in metazoa and yeast. The canonical CTD is characterized by...