Functional specialization of transcription elongation factors (2008)
Georgiy A Belogurov, Rachel A Mooney, Vladimir Svetlov, Robert Landick, Irina Artsimovitch
Allosteric control of the RNA polymerase by the elongation factor RfaH (2007)
Svetlov, Vladimir, Belogurov, Georgiy A., Shabrova, Elena, Vassylyev, Dmitry G., Artsimovitch, Irina
Efficient transcription of long polycistronic operons in bacteria frequently relies on accessory proteins but their molecular mechanisms remain obscure. RfaH is a cellular elongation factor that acts...
Artsimovitch, Irina, Svetlov, Vladimir, Anthony, Larry, Burgess, Richard R., Landick, Robert
Adaptation of bacterial cells to diverse habitats relies on the ability of RNA polymerase to respond to various regulatory signals. Some of these signals are conserved throughout evolution, whereas...
Carter, Heather D., Svetlov, Vladimir, Artsimovitch, Irina
The transcriptional enhancer protein RfaH positively regulates production of virulence factors in Escherichia coli and Salmonella enterica serovar Typhimurium via a cis element, ops. Genes coding for...
Symersky, Jindrich, Perederina, Anna, Vassylyeva, Marina N., Svetlov, Vladimir, Artsimovitch, Irina, Vassylyev, Dmitry G.
Gre factors enhance the intrinsic endonucleolytic activity of RNA polymerase to rescue arrested transcription complexes and are thought to confer the high fidelity and processivity of RNA synthesis....
Perederina, Anna A., Vassylyeva, Marina N., Berezin, Igor A., Svetlov, Vladimir, Artsimovitch, Irina, Vassylyev, Dmitry G.
The Escherichia coli gene encoding the transcription cleavage factor GreB and the Thermus thermophilus gene encoding the anti-GreA transcription factor Gfh1 were cloned and expressed and the purified...
Artsimovitch, Irina, Vassylyeva, Marina N., Svetlov, Dmitri, Svetlov, Vladimir, Perederina, Anna, Igarashi, Noriyuki, ...
Rifamycins, the clinically important antibiotics, target bacterial RNA polymerase (RNAP). A proposed mechanism in which rifamycins sterically block the extension of nascent RNA beyond three...
Artsimovitch, Irina, Svetlov, Vladimir, Anthony, Larry, Burgess, Richard R., Landick, Robert
Adaptation of bacterial cells to diverse habitats relies on the ability of RNA polymerase to respond to various regulatory signals. Some of these signals are conserved throughout evolution, whereas...
Carter, Heather D., Svetlov, Vladimir, Artsimovitch, Irina
The transcriptional enhancer protein RfaH positively regulates production of virulence factors in Escherichia coli and Salmonella enterica serovar Typhimurium via a cis element, ops. Genes coding for...
Allosteric control of the RNA polymerase by the elongation factor RfaH
Svetlov, Vladimir, Belogurov, Georgiy A., Shabrova, Elena, Vassylyev, Dmitry G., Artsimovitch, Irina
Efficient transcription of long polycistronic operons in bacteria frequently relies on accessory proteins but their molecular mechanisms remain obscure. RfaH is a cellular elongation factor that acts...
Perederina, Anna A., Vassylyeva, Marina N., Berezin, Igor A., Svetlov, Vladimir, Artsimovitch, Irina, Vassylyev, Dmitry G.
The GreB transcription cleavage factor of E. coli and the Gfh1 transcription elongation factor of T. thermophilus were cloned, expressed, purified and crystallized. Complete diffraction data sets...
Sevostyanova, Anastasiya, Svetlov, Vladimir, Vassylyev, Dmitry G., Artsimovitch, Irina
RNA polymerase is a target for numerous regulatory events in all living cells. Recent studies identified a few “hot spots” on the surface of bacterial RNA polymerase that mediate its interactions...
Vassylyeva, Marina N., Svetlov, Vladimir, Klyuyev, Sergiy, Devedjiev, Yancho D., Artsimovitch, Irina, Vassylyev, Dmitry G.
The E. coli transcriptional regulator RfaH was cloned, expressed, purified and crystallized and the complex of RfaH with its target DNA oligonucleotide was cocrystallized. Complete diffraction data...
Vassylyeva, Marina N, Svetlov, Vladimir, Dearborn, Altaira D, Klyuyev, Sergiy, Artsimovitch, Irina, Vassylyev, Dmitry G
Bacterial Gre transcript cleavage factors stimulate the intrinsic endonucleolytic activity of RNA polymerase (RNAP) to rescue stalled transcription complexes. They bind to RNAP and extend their...
Functional specialization of transcription elongation factors
Belogurov, Georgiy A, Mooney, Rachel A, Svetlov, Vladimir, Landick, Robert, Artsimovitch, Irina
Elongation factors NusG and RfaH evolved from a common ancestor and utilize the same binding site on RNA polymerase (RNAP) to modulate transcription. However, although NusG associates with RNAP...
Functional regions of the N-terminal domain of the antiterminator RfaH
Belogurov, Georgiy A, Sevostyanova, Anastasia, Svetlov, Vladimir, Artsimovitch, Irina
RfaH is a bacterial elongation factor that increases expression of distal genes in several long, horizontally acquired operons. RfaH is recruited to the transcription complex during RNA chain...