Regional expression and chromosomal localization of the delta opiate receptor gene.
Bzdega, T, Chin, H, Kim, H, Jung, H H, Kozak, C A, Klee, W A
The delta opiate receptor gene has been cloned from the mouse neuroblastoma-rat glioma hybrid cell NG108-15. The clone that we isolated is apparently identical to that reported by Evans et al....
Opiates inhibit adenylate cyclase by stimulating GTP hydrolysis.
Specific, GTP hydrolysis catalyzed by membranes prepared from neuroblastoma--glioma (NG108-15) hybrid cells can be measured in the presence of adenosine-5'-[beta, gamma-imido] triphosphate...
Solubilization of active opiate receptors.
Simonds, W F, Koski, G, Streaty, R A, Hjelmeland, L M, Klee, W A
Receptors that reversibly bind opiates and opioid peptides have been solubilized from brain and neuroblastoma-glioma hybrid cell NG108-15 membranes. Active receptors are specifically solubilized with...
Purification of the opiate receptor of NG108-15 neuroblastoma-glioma hybrid cells.
Simonds, W F, Burke, T R, Rice, K C, Jacobson, A E, Klee, W A
Opiate receptors from NG108-15 neuroblastoma-glioma hybrid cell membranes were purified to apparent homogeneity in a form covalently labeled with the opiate affinity ligand...
Tolerance and dependence evoked by an endogenous opiate peptide.
Lampert, A, Nirenberg, M, Klee, W A
Incubation of neuroblastoma X glioma hybrid cells for 12-97 hr with methionine-enkephalin results in an increase in adenylate cyclase activity [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] that...
Opiate-dependent modulation of adenylate cyclase.
Sharma, S K, Klee, W A, Nirenberg, M
Reactions mediated by the opiate receptors that inhibit adenylate cyclase (EC 4.6.1.1) are closely coupled to subsequent reactions that gradually increase adenylate cyclase activity of neuroblastoma...
Morphine receptors as regulators of adenylate cyclase activity.
Sharma, S K, Nirenberg, M, Klee, W A
Morphine inhibits adenylate cyclase (EC 4.6.1.1) activity of neuroblastoma times glioma hybrid cells. The inhibition is stereospecific and is reversed by the antagonist, naloxone. The relative...
Dual regulation of adenylate cyclase accounts for narcotic dependence and tolerance.
Sharma, S K, Klee, W A, Nirenberg, M
Narcotics affect adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] in two opposing ways, both mediated by the opiate receptor. The first process is the readily reversible inhibition...
Regional expression and chromosomal localization of the delta opiate receptor gene.
Bzdega, T, Chin, H, Kim, H, Jung, H H, Kozak, C A, Klee, W A
The delta opiate receptor gene has been cloned from the mouse neuroblastoma-rat glioma hybrid cell NG108-15. The clone that we isolated is apparently identical to that reported by Evans et al....
Opiates inhibit adenylate cyclase by stimulating GTP hydrolysis.
Specific, GTP hydrolysis catalyzed by membranes prepared from neuroblastoma--glioma (NG108-15) hybrid cells can be measured in the presence of adenosine-5'-[beta, gamma-imido] triphosphate...
Solubilization of active opiate receptors.
Simonds, W F, Koski, G, Streaty, R A, Hjelmeland, L M, Klee, W A
Receptors that reversibly bind opiates and opioid peptides have been solubilized from brain and neuroblastoma-glioma hybrid cell NG108-15 membranes. Active receptors are specifically solubilized with...
Purification of the opiate receptor of NG108-15 neuroblastoma-glioma hybrid cells.
Simonds, W F, Burke, T R, Rice, K C, Jacobson, A E, Klee, W A
Opiate receptors from NG108-15 neuroblastoma-glioma hybrid cell membranes were purified to apparent homogeneity in a form covalently labeled with the opiate affinity ligand...
Tolerance and dependence evoked by an endogenous opiate peptide.
Lampert, A, Nirenberg, M, Klee, W A
Incubation of neuroblastoma X glioma hybrid cells for 12-97 hr with methionine-enkephalin results in an increase in adenylate cyclase activity [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] that...
Opiate-dependent modulation of adenylate cyclase.
Sharma, S K, Klee, W A, Nirenberg, M
Reactions mediated by the opiate receptors that inhibit adenylate cyclase (EC 4.6.1.1) are closely coupled to subsequent reactions that gradually increase adenylate cyclase activity of neuroblastoma...
Morphine receptors as regulators of adenylate cyclase activity.
Sharma, S K, Nirenberg, M, Klee, W A
Morphine inhibits adenylate cyclase (EC 4.6.1.1) activity of neuroblastoma times glioma hybrid cells. The inhibition is stereospecific and is reversed by the antagonist, naloxone. The relative...
Dual regulation of adenylate cyclase accounts for narcotic dependence and tolerance.
Sharma, S K, Klee, W A, Nirenberg, M
Narcotics affect adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] in two opposing ways, both mediated by the opiate receptor. The first process is the readily reversible inhibition...