The purification of the Escherichia coli UvrABC incision system.
Yeung, A T, Mattes, W B, Oh, E Y, Yoakum, G H, Grossman, L
The UvrA, UvrB and UvrC proteins of Escherichia coli have been purified in good yields to homogeneity with rapid three- or four-step purification procedures. The cloned uvrA and uvrB genes were...
Lesion selectivity in blockage of lambda exonuclease by DNA damage.
Various kinds of DNA damage block the 3' to 5' exonuclease action of both E. coli exonuclease III and T4 DNA polymerase. This study shows that a variety of DNA damage likewise inhibits DNA digestion...
Yeung, A T, Mattes, W B, Grossman, L
An examination has been made into the nature of the nucleoprotein complexes formed during the incision reaction catalyzed by the Escherichia coli UvrABC endonuclease when acting on a pyrimidine...
DNA sequence selectivity of guanine-N7 alkylation by nitrogen mustards.
Mattes, W B, Hartley, J A, Kohn, K W
Nitrogen mustards alkylate DNA primarily at the N7 position of guanine. Using an approach analogous to that of the Maxam-Gilbert procedure for DNA sequence analysis, we have examined the relative...
Mechanisms of DNA sequence selective alkylation of guanine-N7 positions by nitrogen mustards.
Kohn, K W, Hartley, J A, Mattes, W B
Quantitative determinations were carried out of the relative reaction rates of several nitrogen mustards at various guanine-N7 positions in DNA fragments of known sequence. The findings suggest...
Yoakum, G H, Yeung, A T, Mattes, W B, Grossman, L
We have constructed a hybrid pKC30-uvrA plasmid (pGHY5003) in which transcription of the uvrA gene can be induced under pL control to amplify the uvrA gene product to 7% of cellular protein. To...
Enzymatic properties of purified Escherichia coli uvrABC proteins.
Yeung, A T, Mattes, W B, Oh, E Y, Grossman, L
The cloned uvrA and uvrB genes of Escherichia coli K-12 were amplified by linkage to the PL promoter of plasmid pKC30. The uvrC gene was amplified in the high-copy-number plasmid pRLM 24. The three...
The purification of the Escherichia coli UvrABC incision system.
Yeung, A T, Mattes, W B, Oh, E Y, Yoakum, G H, Grossman, L
The UvrA, UvrB and UvrC proteins of Escherichia coli have been purified in good yields to homogeneity with rapid three- or four-step purification procedures. The cloned uvrA and uvrB genes were...
Lesion selectivity in blockage of lambda exonuclease by DNA damage.
Various kinds of DNA damage block the 3' to 5' exonuclease action of both E. coli exonuclease III and T4 DNA polymerase. This study shows that a variety of DNA damage likewise inhibits DNA digestion...
Yeung, A T, Mattes, W B, Grossman, L
An examination has been made into the nature of the nucleoprotein complexes formed during the incision reaction catalyzed by the Escherichia coli UvrABC endonuclease when acting on a pyrimidine...
DNA sequence selectivity of guanine-N7 alkylation by nitrogen mustards.
Mattes, W B, Hartley, J A, Kohn, K W
Nitrogen mustards alkylate DNA primarily at the N7 position of guanine. Using an approach analogous to that of the Maxam-Gilbert procedure for DNA sequence analysis, we have examined the relative...
Mechanisms of DNA sequence selective alkylation of guanine-N7 positions by nitrogen mustards.
Kohn, K W, Hartley, J A, Mattes, W B
Quantitative determinations were carried out of the relative reaction rates of several nitrogen mustards at various guanine-N7 positions in DNA fragments of known sequence. The findings suggest...
Yoakum, G H, Yeung, A T, Mattes, W B, Grossman, L
We have constructed a hybrid pKC30-uvrA plasmid (pGHY5003) in which transcription of the uvrA gene can be induced under pL control to amplify the uvrA gene product to 7% of cellular protein. To...
Enzymatic properties of purified Escherichia coli uvrABC proteins.
Yeung, A T, Mattes, W B, Oh, E Y, Grossman, L
The cloned uvrA and uvrB genes of Escherichia coli K-12 were amplified by linkage to the PL promoter of plasmid pKC30. The uvrC gene was amplified in the high-copy-number plasmid pRLM 24. The three...