Schalkwijk, J, Van Den Berg, W B, Van De Putte, L B, Joosten, L A
Several enzymes and other proteins were made cationic either by coupling to polylysine or by shielding of anionic sites. These cationic proteins, all having an isoelectric point greater than 8.5...
Van Den Berg, W B, Van De Putte, L B, Zwarts, W A, Joosten, L A
We studied the influence of antigenic charge on the handling of intraarticular antigen by the joint and on the ability of the antigen to induce chronic arthritis. Three different antigens were used:...
Schalkwijk, J, Van Den Berg, W B, Van De Putte, L B, Joosten, L A
Several enzymes and other proteins were made cationic either by coupling to polylysine or by shielding of anionic sites. These cationic proteins, all having an isoelectric point greater than 8.5...
Van Den Berg, W B, Van De Putte, L B, Zwarts, W A, Joosten, L A
We studied the influence of antigenic charge on the handling of intraarticular antigen by the joint and on the ability of the antigen to induce chronic arthritis. Three different antigens were used:...
Allergic arthritis induced by cationic proteins: role of molecular weight.
Van Lent, P L, Dekker, C, Mosterd, J, Van Den Berg, W B
Previous studies have shown that chronic murine allergic arthritis can only be induced with cationized BSA, related to excellent retention of the cationic antigen in the joint. We now investigate the...
Anergy of antigen-specific T lymphocytes is a potent mechanism of intravenously induced tolerance.
Jacobs, M J, Van Den Hoek, A E, Van De Putte, L B, Van Den Berg, W B
Intravenous (i.v.) injection of an antigen before immunization has been shown to be a potent way to induce suppression at the T-cell level. In this study we demonstrate an almost complete suppression...
Role of IL-2 and IL-4 in exacerbations of murine antigen-induced arthritis.
Jacobs, M J, Van Den Hoek, A E, Van Lent, P L, Van De Loo, F A, Van De Putte, L B, Van Den Berg, W B
In this study the roles of different T-cell subsets, and produced cytokines, were investigated in an animal model for acute exacerbations. Flare-up reactions are inducible in the chronic phase of a...
Jacobs, M J, Van Den Hoek, A E, Van De Putte, L B, Van Den Berg, W B
T-cell vaccination using antigen-specific lines or clones has been shown to be effective in down-regulating immunity in various experimental autoimmune models. Anti-idiotypic networks developing...
Cell migration studies in the adoptive transfer of adjuvant arthritis in the Lewis rat.
Van De Langerijt, A G, Volsen, S G, Hicks, C A, Craig, P J, Billingham, M E, Van Den Berg, W B
Adjuvant arthritis (AA) can be induced in Lewis rats by a single injection of either heat-killed Mycobacterium tuberculosis or the lipoidal amine CP20961. Concanavalin A (Con A)-stimulated T cells...
Van Lent, P L, Van Den Berg, W B, Schalkwijk, J, Van De Putte, L B
In order to define the antigenic properties necessary for sustained allergic arthritis, we prepared a range of differently charged bovine serum albumin (BSA) species with increasing isoelectric...
Exacerbation of antigen-induced arthritis after challenge with intravenous antigen.
Van De Putte, L B, Lens, J W, Van Den Berg, W B, Kruijsen, M W
The occurrence and morphology of flare-up of arthritis after intravenous (i.v.) antigen administration was investigated in mice with on-going antigen-induced arthritis (AIA). Intravenous injection of...
Van Den Berg, W B, Haasakker, T C, Van Maarsseveen, A C, Scheper, R J
A chronic inflammation model is described which allows the study of the relationship between the level of specific cell-mediated and humoral immunity to the triggering antigen, and the presence of...
Van Den Berg, W B, Haasakker, T C, Bax, J, Scheper, R J
Studies were carried out in order to explain the often small contribution of eosinophils to immunologically mediated chronic inflammatory reactions. A chronic inflammation model was used in which...
Lymphokine-mediated stimulation of eosinophil migration in the guinea-pig.
Van Den Berg, W B, Van Tuyl, J, Sminia, P, Jentjens, T M, Scheper, R J
Guinea-pig lymphokines were shown to stimulate the migration of eosinophils from capillary tubes. Eosinophil migration stimulatory activity was produced by Freund's complete adjuvant (FCA)-sensitized...
Van Beusekom, H J, Van De Putte, L B, Van Den Berg, W B, Van Den Broek, W J, Buijs, W C
The fate of a second intra-articularly (i.a.) injected dose of bovine serum albumin (BSA) in an already established BSA-induced knee-joint inflammation was compared with that of a paired first...
Van Beusekom, H J, Van De Putte, L B, Van Den Berg, W B, Van Den Broek, W J
We studied (i) the inflammatory response induced by intra-articular (i.a.) injection of 2.5 and 0.5 mg 125I-labelled bovine serum albumin (125I-BSA) into the paired knee joints of rabbits immunized...
Van Den Berg, W B, Van De Loo, F A, Zwarts, W A, Otterness, I G
Murine recombinant interleukin 1 (IL1) was tested for its ability to affect intact murine articular cartilage. IL1 caused enhanced proteoglycan degradation and severe inhibition of chondrocyte...
Schalkwijk, J, Joosten, L A, Van Den Berg, W B, Van De Putte, L B
Mice with the beige mutation are known to be deficient for polymorphonuclear leucocyte (PMN) elastase and cathepsin G and can therefore be used as a model for protease dependence of tissue...
Van Lent, P L, Wilms, F H, Van Den Berg, W B
Short term immobilisation (seven days) of an antigen induced arthritic knee joint of the mouse led to attachment of polymorphonuclear leucocytes to the cartilage surface and severe cartilage damage....
Van De Loo, A A, Van Den Berg, W B
Murine recombinant interleukin 1 was injected intra-articularly into mice. It induced a clear effect on patellar cartilage within 24 hours. A low dose of interleukin 1 (1 ng) elicited a significant...
Antigen induced arthritis in beige (Chediak-Higashi) mice.
Schalkwijk, J, Joosten, L A, Van Den Berg, W B, Van De Putte, L B
Mice with the beige mutation, which are known to be deficient for leucocyte elastase and cathepsin G, were used to investigate the role of neutral proteases in a model for antigen induced arthritis....
Van Beuningen, H M, Van Der Kraan, P M, Arntz, O J, Van Den Berg, W B
The modulation of interleukin 1 (IL-1) effects on proteoglycan metabolism in intact murine patellar cartilage by transforming growth factor beta (TGF-beta) was investigated in vitro and in vivo. In...
Van Der Kraan, P M, Vitters, E L, Postma, N S, Verbunt, J, Van Den Berg, W B
OBJECTIVES--The exact regulation of the synthesis of cartilage specific molecules, such as collagen type II and aggrecan, by articular chondrocytes is unknown, but growth factors and hormones...
Effects of methotrexate on glycosaminoglycan production by scleroderma fibroblasts in culture.
Van Den Hoogen, F H, Van Der Kraan, P M, Boerbooms, A M, Van Den Berg, W B, Van Lier, H J, Van De Putte, L B
OBJECTIVE--To determine the effects of increasing concentrations of methotrexate on the proliferation and glycosaminoglycan (GAG) synthesis of cultured dermal fibroblasts from patients with...
Glansbeek, H L, Van Der Kraan, P M, Vitters, E L, Van Den Berg, W B
OBJECTIVES--Transforming growth factor beta (TGF-beta) is a multipotent regulator of cell proliferation and extracellular matrix production. The effect of TGF-beta on chondrocyte matrix production...
Verschure, P J, Joosten, L A, Van Der Kraan, P M, Van Den Berg, W B
OBJECTIVE--Disturbed anabolic signalling might contribute to the decreased chondrocyte proteoglycan (PG) synthesis during joint inflammation. Articular cartilage obtained from mouse knee joints with...
Van Beuningen, H M, Van Der Kraan, P M, Arntz, O J, Van Den Berg, W B
OBJECTIVES--Transforming growth factor-beta (TGF-beta) has been shown to antagonise interleukin-1 (IL-1) effects in different systems. Investigations were carried out to study whether TGF-beta 1...
Van Der Kraan, P M, Vitters, E L, Van Den Berg, W B
The effect of transforming growth factor beta (TGF beta) on proteoglycan synthesis and degradation in anatomically intact articular cartilage of murine patellae was studied. Exogenously added TGF...
Verschure, P J, Van Marle, J, Joosten, L A, Van Den Berg, W B
OBJECTIVE--To examine the distribution of insulin like growth factor-1 (IGF-1) receptors and the biological response to IGF-1 stimulation in articular cartilage of normal mouse knee joints and...
Verschure, P J, Joosten, L A, Van De Loo, F A, Van Den Berg, W B
OBJECTIVE--To investigate the involvement of interleukin-1 (IL-1) in the induction or maintenance of the insulin-like growth factor 1 (IGF-1) non-responsive state of chondrocytes during experimental...
Holthuysen, A, Bayne, E, Singer, I, ...
OBJECTIVE—Murine antigen induced arthritis (AIA) is a chronic, smouldering inflammation. Flares of arthritis can be induced by antigen rechallenge or exposure to inflammatory mediators like...
Joosten, L., Helsen, M., Van Den Berg, W. B
OBJECTIVE—The goal of this study was to investigate the role of endogenous interleukin 12 (IL12) in acute murine streptococcal cell wall (SCW) arthritis. METHODS—C57black/6 mice were injected...
Barrera, P, Joosten, L, Den Broeder, A A, Van Den Berg, W B
OBJECTIVES—To study the short term effects of a single dose of D2E7, a fully human anti-tumour necrosis factor (TNFα) monoclonal antibody (mAb), on the local and systemic homeostasis of...
Scharstuhl, A, Van Beuningen, H M, Vitters, E, Van Der Kraan, P M, Van Den Berg, W B
Objective: To investigate if a difference exists between young and old mice in the response of articular cartilage to interleukin 1 (IL1) and transforming growth factor ß (TGFß) alone or in...
Blom, A, Holthuysen, A, Jacobs, C, Van Den Berg, W B
Background: Recently, it has been found that collagen type II arthritis susceptible mouse strains are hyperreactive to immune complexes (ICs), locally deposited into their knee joints.
Van Den Berg, W. B, Joosten, L., Kollias, G.
Chronic arthritis is characterised by persistent joint inflammation and concomitant joint destruction. Using murine arthritis models and neutralising antibodies as well as cytokine specific knockout...
Arguments for interleukin 1 as a target in chronic arthritis
Tumour necrosis factor (TNF) and interleukin 1 (IL1) are considered as master cytokines in chronic, destructive arthritis. Therapeutic approaches in rheumatic arthritis (RA) patients so far mainly...
Metselaar, J, Van Den Berg, W B, Holthuysen, A, Wauben, M, Storm, G
Objective: To investigate the effect of a single intravenous treatment with glucocorticoids (GC) encapsulated in long-circulating PEG-liposomes on both joint inflammation and cartilage destruction...
Van De Loo, F. A., Kuiper, S., Van Enckevort, F. H., Arntz, O. J., Van Den Berg, W. B.
Using interleukin (IL)-6-deficient (IL-6(0/0) mice or wild-type mice, we investigated the controversial role of IL-6 in joint inflammation and cartilage pathology during zymosan-induced arthritis...
Role of interleukin 1 in antigen-induced exacerbations of murine arthritis.
Van De Loo, A. A., Arntz, O. J., Bakker, A. C., Van Lent, P. L., Jacobs, M. J., Van Den Berg, W. B.
The mechanism underlying the chronic and intermittent course of rheumatoid arthritis is not elucidated. In the present study, the role of interleukin 1 (IL-1) was investigated in exacerbations of...
Van Lent, P. L., Van De Putte, L. B., Van Den Berg, W. B.
The early and late effects of short-term immobilization on arthritic joints have been studied. Knee joints of mice in which an antigen-induced unilateral arthritis was elicited were immobilized in...
Radstake, T, Pesman, G, Blom, A, Sweep, F, Ronnelid, J, ...
Objective: To assess whether DC from RA produce altered cytokine levels and whether this is regulated by triggering of Fc gamma receptors (FcγR).
Van Der Kraan, P. M., Vitters, E. L., Van De Putte, L. B., Van Den Berg, W. B.
Male, 10-week-old C57B1 10 mice received a single intraarticular injection in the knee joints with papain, iodoacetate, or collagenase. This led to osteoarthritic lesions, such as matrix depletion,...
Van Den Broek, M. F., Van Den Berg, W. B., Van De Putte, L. B., Severijnen, A. J.
Intra-articular injection of cell walls from the gram-positive bacterium Streptococcus pyogenes induces an arthritis in both streptococcal cell wall (SCW)-primed and naive mice. This joint...
Lafeber, F. P., Van Der Kraan, P. M., Van Roy, H. L., Vitters, E. L., Huber-Bruning, O., Van Den Berg, W. B., ...
Proteoglycan synthesis of mild-to-moderate osteoarthritic human knee cartilage was compared with that of normal cartilage of the same donor. Immediately after cartilage was obtained, the synthesis...
Van Lent, P. L., Van Den Bersselaar, L. A., Van Den Hoek, A. E., Van De Loo, A. A., Van Den Berg, W. B.
A novel cationic immune-complex-mediated arthritis (ICA) model was developed in mice. The highly cationic protein lysozyme was coupled to poly-L-lysine (PLL) and injected intra-articularly into the...
In vivo role of phagocytic synovial lining cells in onset of experimental arthritis.
Van Lent, P. L., Van Den Hoek, A. E., Van Den Bersselaar, L. A., Spanjaards, M. F., Van Rooijen, N., Dijkstra, C. D., ...
The in vivo role of phagocytic synovial lining cells (SLC) was studied in acute experimental arthritis in the mouse. SLCs were selectively depleted by injecting liposomes encapsulating the drug...
Van Den Berg, W. B., Van De Putte, L. B.
Intraarticular injection of cationic bovine serum albumin (BSA) induces a chronic arthritis in immunized mice, whereas the negatively charged native BSA fails to cause a protracted joint...
Van Den Berg, W. B., Joosten, L. A., Van De Putte, L. B., Zwarts, W. A.
Chronicity of murine allergic arthritis depends on the charge-mediated retention of the cationic antigen in the joint. The authors examined whether arthritis induced with the positively charged...
Regulation of resistance against adjuvant arthritis in the Fisher rat.
Van De Langerijt, A G, Van Lent, P L, Hermus, A R, Van De Putte, L B, Van Den Berg, W B
Inbred female Lewis (LEW/N) rats develop a severe chronic arthritis in the adjuvant arthritis (AA) model, histocompatible Fisher (F344/N) rats are resistant and germ-free Fishers (GF F344) are again...
Jacobs, M J, Van Den Hoek, A E, Van De Putte, L B, Van Den Berg, W B
The induction of tolerance, particularly by intervention before established immunity, is widely accepted. We studied the effects of intravenous (i.v.) administration of hen egg lysozyme (HEL), before...
Accelerated onset of collagen-induced arthritis by remote inflammation.
Joosten, L A, Helsen, M M, Van Den Berg, W B
Collagen-induced arthritis (CIA) in DBA-1 lac/J mice often has a low incidence, with gradual disease expression occurring over a broad time span (between days 35 and 70). The exact mechanisms...
Van Den Broek, M F, Van Bruggen, M C, Stimpson, S A, Severijnen, A J, Van De Putte, L B, Van Den Berg, W B
One i.p. injection of a sterile suspension of streptococcal cell walls (SCW) induces chronic erosive polyarthritis in susceptible Lewis rats, but not in resistant F344 or nude Lewis rats. Because...
Susceptibility to adjuvant arthritis: relative importance of adrenal activity and bacterial flora.
Van De Langerijt, A G, Van Lent, P L, Hermus, A R, Sweep, C G, Cools, A R, Van Den Berg, W B
Previous studies on the regulation of bacterial-induced arthritis in rats have focused on endocrine aspects as well as differences in T cell immunity against bacterial epitopes. We analysed the role...
Amelioration of established murine collagen-induced arthritis with anti-IL-1 treatment.
Van Den Berg, W B, Joosten, L A, Helsen, M, Van De Loo, F A
Inflammatory cytokines have been implicated in the pathogenesis of rheumatoid arthritis. To validate a key role for IL-1 in arthritic processes we have studied the protective effect of neutralizing...
Lens, J W, Van Den Berg, W B, Van De Putte, L B
Intravenous (i.v.) administration of 300 micrograms methylated BSA (mBSA) to C57-Black mice with unilateral antigen-induced arthritis (AIA) of 4-6 weeks duration resulted in a reproducible flare-up...
Lens, J W, Van Den Berg, W B, Van De Putte, L B, Berden, J H, Lems, S P
Intravenous injection of methylated bovine serum albumin (mBSA) into mice with unilateral chronic mBSA-induced arthritis (AIA) causes a flare-up of the joint inflammation without affecting the...
Van Den Broek, M F, Van Den Berg, W B, Arntz, O J, Van De Putte, L B
Although different models for rheumatoid arthritis have been studied, the pathogenesis in humans remains unknown. A possible mechanism is the crossreactivity between bacterial components and the...
Monoclonal anti-Ia antibodies suppress the flare up reaction of antigen induced arthritis in mice.
Van Den Broek, M F, Van Den Berg, W B, Van De Putte, L B
Intravenous injection of methylated bovine serum albumin (mBSA) in mice with a unilateral antigen-induced arthritis induced with mBSA causes a flare up of the inflammation in the arthritic but not in...
Flare-up of experimental arthritis in mice with murine recombinant IL-1.
Van De Loo, A A, Arntz, O J, Van Den Berg, W B
Intra-articular injections of murine recombinant IL-1 (mrIL-1) during the chronic phase of antigen-induced arthritis (AIA) induced a flare-up of the smouldering inflammation. The exacerbation was...
Van Den Broek, M F, Van Bruggen, M C, Koopman, J P, Hazenberg, M P, Van Den Berg, W B
Streptococcal cell wall (SCW)-induced arthritis is a chronic, erosive polyarthritis that can be induced in susceptible Lewis rats by one i.p. injection of an aqueous, sterile suspension of SCW. F344...
Flare-up of antigen-induced arthritis in mice after challenge with oral antigen.
Lens, J W, Van Den Berg, W B, Van De Putte, L B
Mice with unilateral chronic mBSA-induced arthritis were orally challenged with mBSA. Three hours after antigen challenge clear flare-up of the chronic arthritis was demonstrable as detected by an...
Lubberts, E, Joosten, L A B, Helsen, M M A, Bakker, A C, Xing, Z, ...
We studied the effects of local IL-10 application, introduced by a recombinant human type 5 adenovirus vector, in the mouse knee joint during the early phase of CIA. One intra-articular injection...
Van Den Berg, W. B., Van Beusekom, H. J., Van De Putte, L. B., Zwarts, W. A.
Antigen localization after intraarticular antigen injection was studied in immune and nonimmune mice using autoradiographic and immunofluorescence techniques on whole joint sections. After...
Radstake, T, Blom, A, Sloetjes, A, Pesman, G, Engelen, L, ...
Objectives: To investigate potential differences in phenotype and behaviour of immature (iDC) and mature dendritic cells (mDC) from patients with RA and healthy subjects.
Barrera, P, Smeets, R, Pesman, G, Van Den Berg, W B, ...
Background: Dendritic cells orchestrate pivotal immunological processes mediated by the production of cytokines and chemokines.
Radstake, T, Ten, B, De Waal, Malefijt M, Looman, M, Figdor, C, ...
Background: Dendritic cells (DC) have a role in the regulation of immunity and tolerance, attracting inflammatory cells by the production of various chemokines (CK). Fc gamma receptors (FcγR) may be...
Span, P, Sloetjes, A, Radstake, T, Heuvel, J, ...
Objective: To assess the expression and localisation of the new metalloproteinase inhibitor RECK, an inhibitor of matrix metalloproteinase-14 (MMP-14) secretion and activity, in the synovial membrane...