Radical changes to chlamydial taxonomy are not necessary just yet (letter and reply) (2001)
Schachter, J., Stephens, R.S., Timms, P., Kuo, C., Bavoil, P.M., Birkelund, S., ...
Torian, B E, Flores, B M, Stroeher, V L, Hagen, F S, Stamm, W E
A gamma gt11 cDNA library was constructed from poly(U)-Sepharose-selected Entamoeba histolytica trophozoite RNA in order to clone and identify surface antigens. The library was screened with rabbit...
Susceptibility of Chlamydia trachomatis to Chlorhexidine Gluconate Gel
Lampe, M. F., Ballweber, L. M., Stamm, W. E.
To identify topical antimicrobial preparations which may be effective in preventing the transmission of sexually transmitted diseases, we examined the activity of chlorhexidine gluconate (CHG)...
Lampe, M. F., Ballweber, L. M., Isaacs, C. E., Patton, D. L., Stamm, W. E.
The development of new methods for prevention of sexually transmitted Chlamydia trachomatis infection is a top public health priority. Topical self-administered vaginal microbicides represent one...
Stapleton, A. E., Stroud, M. R., Hakomori, S. I., Stamm, W. E.
Women with a history of recurrent Escherichia coli urinary tract infections (UTIs) are significantly more likely to be nonsecretors of blood group antigens than are women without such a history, and...
Ballweber, L. M., Jaynes, J. E., Stamm, W. E., Lampe, M. F.
Topically applied microbicides that eradicate pathogens at the time of initial exposure represent a powerful strategy for the prevention of sexually transmitted infections. To aid in the further...
Ofloxacin versus trimethoprim-sulfamethoxazole for treatment of acute cystitis.
Hooton, T M, Latham, R H, Wong, E S, Johnson, C, Roberts, P L, Stamm, W E
We compared the safety and efficacies of ofloxacin and trimethoprim-sulfamethoxazole for the treatment of acute uncomplicated cystitis in women enrolled in a multicenter study. Data from three...
Characterization of the hemolytic activity of Haemophilus ducreyi.
Totten, P A, Norn, D V, Stamm, W E
H. ducreyi is the causative agent of chancroid, a genital ulcer disease most prevalent in developing countries. Chancroid enhances the heterosexual transmission of human immunodeficiency virus and is...
Lampe, M F, Wong, K G, Kuehl, L M, Stamm, W E
We have identified two families of novel Chlamydia trachomatis isolates with amino acid changes within the major outer membrane protein (MOMP) variable domains: one family of Da, D*, and D- and one...
Lichtenwalner, A B, Patton, D L, Cosgrove Sweeney, Y T, Gaur, L K, Stamm, W E
The macaque model of chlamydial pelvic inflammatory disease (PID) demonstrates individual variability in the time of onset of intrapelvic adhesions. Some animals develop adhesions rapidly, within 2...
Tartaglione, T A, Johnson, C R, Brust, P, Opheim, K, Hooton, T M, Stamm, W E
Vaginal colonization with Escherichia coli is an integral step in the development of acute cystitis, and persistent vaginal coliform colonization may also be a predisposing step to recurrent urinary...
Ineffectiveness and toxicity of BCG vaccine for the prevention of recurrent genital herpes.
Douglas, J M, Vontver, L A, Stamm, W E, Reeves, W C, Critchlow, C, Remington, M L, ...
One hundred fifty-five patients with genital herpes were enrolled in a double-blind, placebo-controlled trial comparing 0.1 ml of intradermal BCG vaccine with placebo for the prevention of recurrent...
The MICs of three new antimicrobial agents of different classes, U-70138F, roxithromycin (RU 965), and ofloxacin (ORF 18489), versus Chlamydia trachomatis in McCoy cell cultures were 0.25, 0.8, and...
Latham, R H, Zeleznik, D, Minshew, B H, Schoenknecht, F D, Stamm, W E
beta-Lactamase production and MIC determinations for penicillin, methicillin, and cephalothin were assessed for 67 strains of Staphylococcus saprophyticus and correlated with results of disk...
In vitro susceptibilities of Campylobacter-like organisms to twenty antimicrobial agents.
Flores, B M, Fennell, C L, Holmes, K K, Stamm, W E
We determined MICs of 20 antimicrobial agents for 50 representative strains of four subgroups of Campylobacter-like organisms (CLOs) by agar dilution. Ampicillin, gentamicin, doxycycline,...
Ofloxacin versus doxycycline for treatment of cervical infection with Chlamydia trachomatis.
Hooton, T M, Batteiger, B E, Judson, F N, Spruance, S L, Stamm, W E
Women with culture-proven Chlamydia trachomatis cervical infection were randomized to receive either ofloxacin (300 mg) or doxycycline (100 mg), orally twice daily for 7 days. All 56 had negative...
Direct antimicrobial susceptibility testing for acute urinary tract infections in women.
Johnson, J R, Tiu, F S, Stamm, W E
Despite its theoretical advantages, direct antimicrobial susceptibility testing (DST) of urine specimens remains controversial largely because of concerns regarding its accuracy, particularly with...
Suchland, K L, Counts, J M, Stamm, W E
The last decade has witnessed the development of a wide variety of diagnostic tests for Chlamydia trachomatis. In order to determine what laboratory methods are being used to detect C. trachomatis...
Hooton, T M, Johnson, C, Winter, C, Kuwamura, L, Rogers, M E, Roberts, P L, ...
We compared the safety and efficacy of a single 400-mg dose of ofloxacin, ofloxacin (200 mg) once daily for 3 days, and trimethoprim-sulfamethoxazole (160:800 mg) twice daily for 7 days for the...
Johnson, J R, Goullet, P, Picard, B, Moseley, S L, Roberts, P L, Stamm, W E
We determined the carboxylesterase B electrophoretic profiles of 74 blood isolates of Escherichia coli from patients with urosepsis. Most strains (64%) exhibited the B2 electrophoretic pattern. P...
McFarland, L V, Elmer, G W, Stamm, W E, Mulligan, M E
To determine whether strain-specific differences in immunoblot type, enterotoxin production, or cytotoxin production correlated with clinical presentation of Clostridium difficile infection, we...
Torian, B E, Reed, S L, Flores, B M, Creely, C M, Coward, J E, Vial, K, ...
A surface antigen (EH-96) of Entamoeba histolytica was demonstrated to be a plasma membrane antigen by immunoprecipitation of metabolically 35S-labeled antigen from live trophozoites, Triton X-114...
Johnson, J R, Moseley, S L, Roberts, P L, Stamm, W E
To assess the role of aerobactin as a virulence factor among uropathogenic Escherichia coli, we determined the prevalence, location, and phenotypic expression of aerobactin determinants among 58 E....
A Chlamydia trachomatis D* serovariant strain was found to be mixed with an F serovar strain in a clinical specimen. By using a monoclonal antibody which neutralizes serovar F infectivity in hamster...
Clear broth and plate media for culture of Haemophilus ducreyi.
Using catalase as a source of heme, we have developed both clear plate and broth media for the growth of Haemophilus ducreyi, the causative agent of chancroid. In the broth medium, the growth phase...
Ligase chain reaction to detect Chlamydia trachomatis infection of the cervix.
Schachter, J, Stamm, W E, Quinn, T C, Andrews, W W, Burczak, J D, Lee, H H
We performed a multicenter evaluation of ligase chain reaction (LCR) in the diagnosis of Chlamydia trachomatis infection of the cervix. This LCR provides an amplification of target sequences within...
"Campylobacter cinaedi" bacteremia: case report and laboratory findings.
Cimolai, N, Gill, M J, Jones, A, Flores, B, Stamm, W E, Laurie, W, ...
"Campylobacter cinaedi" was isolated from the blood of a 29-year-old homosexual man with previously diagnosed acquired immune deficiency syndrome. Subculturing of the organism was achieved with the...
Bauwens, J E, Clark, A M, Loeffelholz, M J, Herman, S A, Stamm, W E
To determine the accuracy of a recently developed polymerase chain reaction (PCR) urine assay to detect Chlamydia trachomatis urethral infection in men, we obtained urethral swabs and first-catch...
Bauwens, J E, Clark, A M, Stamm, W E
We evaluated a prototype polymerase chain reaction (PCR)-based assay for Chlamydia trachomatis developed by Roche Molecular Systems to detect endocervical infection in women. Of 587 endocervical...
Cles, L D, Bruch, K, Stamm, W E
Using purified elementary bodies of 14 Chlamydia trachomatis serovars in an in vitro assay, we compared the staining characteristics of six commercially available monoclonal antibody reagents used...
Campylobacter cinaedi is normal intestinal flora in hamsters.
Gebhart, C J, Fennell, C L, Murtaugh, M P, Stamm, W E
During the course of studies to reproduce proliferative enteritis in hamsters, Campylobacter cinaedi was recovered from the feces of the majority of healthy hamsters obtained from two commercial...
Use of HL cells for improved isolation and passage of Chlamydia pneumoniae.
We compared growth of the recently discovered respiratory pathogen Chlamydia pneumoniae in McCoy, HeLa 229, BHK-21, and HL cells. When cells were not pretreated with DEAE-dextran, HL cells had...
Flores, B M, Garcia, C A, Stamm, W E, Torian, B E
Monoclonal antibodies to Naegleria fowleri and Acanthamoeba polyphaga were analyzed by enzyme-linked immunosorbent assay, indirect immunofluorescence microscopy, and fluorescence flow cytometry to...
Schwebke, J R, Stamm, W E, Handsfield, H H
Endocervical infections due to Chlamydia trachomatis remain difficult to diagnose due to the lack of an inexpensive, rapid, and accurate test. We evaluated an alternative strategy for diagnosis in...
Barnes, R C, Wang, S P, Kuo, C C, Stamm, W E
The technical complexity of determining the serovar of Chlamydia trachomatis strains has limited the use of serotyping in clinical and epidemiologic studies. We developed a simple method for rapidly...
Isolation of "Campylobacter hyointestinalis" from a human.
Fennell, C L, Rompalo, A M, Totten, P A, Bruch, K L, Flores, B M, Stamm, W E
We isolated "Campylobacter hyointestinalis" from the rectal culture of a homosexual man with proctitis. Phenotypic characterization of the isolate was confirmed by DNA hybridization by using the...
Totten, P A, Patton, C M, Tenover, F C, Barrett, T J, Stamm, W E, Steigerwalt, A G, ...
A total of 593 strains of thermophilic Campylobacter species were isolated either from humans with diarrhea or from poultry in King County, Washington. Of these strains, 98 (52 hippurate-positive...
Ng, V L, Hadley, W K, Fennell, C L, Flores, B M, Stamm, W E
A bisexual human immunodeficiency virus-seropositive male had successive bacteremias with "Campylobacter cinaedi" and "Campylobacter fennelliae." Because final identification of both isolates was not...
Rectal swab cultures for Clostridium difficile surveillance studies.
McFarland, L V, Coyle, M B, Kremer, W H, Stamm, W E
We compared the recovery of Clostridium difficile from hospitalized patients by two collection methods: rectal swabs and stool cultures. Rectal swab cultures were as sensitive as stool cultures and...
Simplified microtiter cell culture method for rapid immunotyping of Chlamydia trachomatis.
Serotyping of Chlamydia trachomatis strains usually requires three to six serial passages in shell vials to attain sufficient antigen for typing procedures. To circumvent this problem, we developed a...
Use of a urine enzyme immunoassay as a diagnostic tool for Chlamydia trachomatis urethritis in men.
Schwebke, J R, Clark, A M, Pettinger, M B, Nsubga, P, Stamm, W E
We collected first-voided urine specimens from 659 males attending a sexually transmitted disease clinic and performed both enzyme immunoassay (EIA) for detection of chlamydial antigen and leukocyte...
Clark, A, Stamm, W E, Gaydos, C, Welsh, L, Quinn, T C, Schachter, J, ...
To evaluate the AntigEnz Chlamydia enzyme immunoassay (EIA) (Baxter/Bartels, Issaquah, Wash.), we studied 320 men and 1,209 women attending clinics for sexually transmitted diseases in Baltimore, San...
Antigen detection for the diagnosis of gonorrhea.
Stamm, W E, Cole, B, Fennell, C, Bonin, P, Armstrong, A S, Herrmann, J E, ...
We compared the diagnostic value of an enzyme immunoassay method for detection of gonococcal antigen in genital secretions with culture results and direct Gram stain for Neisseria gonorrhoeae in...
Stamm, W E, Tam, M, Koester, M, Cles, L
We compared two methods for identification of Chlamydia trachomatis inclusions in McCoy cell monolayers: conventional iodine staining and immunofluorescence staining with monoclonal antibodies...
Enzyme immunoassay for detection of antibody-coated bacteria.
Stamm, W E, Cutter, B E, Grootes-Reuvecamp, G A
To quantitatively evaluate factors potentially affecting antibody coating of bacteria in urine, we developed an assay with enzyme-linked rather than fluorescein-conjugated immunoglobulin. Using the...
Microtest procedure for isolation of Chlamydia trachomatis.
Yoder, B L, Stamm, W E, Koester, C M, Alexander, E R
Management of patients potentially infected with Chlamydia trachomatis has been hampered by the cost and time required to perform Chlamydia cultures. To isolate C. trachomatis, we developed a...
Lampe, M F, Suchland, R J, Stamm, W E
During studies in which we serotyped large numbers of Chlamydia trachomatis clinical isolates by using monoclonal antibodies, three novel serological variants (D-, D*, and I-) were identified. To...
Haemophilus ducreyi attaches to and invades human epithelial cells in vitro.
Totten, P A, Lara, J C, Norn, D V, Stamm, W E
Haemophilus ducreyi is a sexually transmitted pathogen that causes genital ulcers and inguinal adenopathy. Because chancroidal ulcers are most commonly located on the foreskins of uncircumcised...
Flores, B M, Fennell, C L, Kuller, L, Bronsdon, M A, Morton, W R, Stamm, W E
Campylobacter cinaedi and C. fennelliae have been associated with proctocolitis, bacteremia, and asymptomatic rectal infection, primarily in homosexual men. To more directly assess the pathogenic...
Stapleton, A, Nudelman, E, Clausen, H, Hakomori, S, Stamm, W E
Women with a history of recurrent Escherichia coli urinary tract infections (UTIs) are two to three times more likely to be nonsecretors of histo-blood group antigens than are women without such a...
Lampe, M. F., Rohan, L. C., Skinner, M. C., Stamm, W. E.
Commonly used “inactive” pharmaceutical excipients were tested in a previously developed minimum cidal concentration assay to assess their ability to kill Chlamydia trachomatis topically. Sixteen...
Sánchez, J, Gotuzzo, E, Escamilla, J, Carrillo, C, Phillips, I A, Barrios, C, ...
OBJECTIVES. This study examined the prevalences of antibodies to Treponema pallidum, Chlamydia trachomatis, and herpes simplex virus type 2 in a sample of Peruvian adults. METHODS. Among adults...
Torian, B E, Flores, B M, Stroeher, V L, Hagen, F S, Stamm, W E
A gamma gt11 cDNA library was constructed from poly(U)-Sepharose-selected Entamoeba histolytica trophozoite RNA in order to clone and identify surface antigens. The library was screened with rabbit...
Susceptibility of Chlamydia trachomatis to Chlorhexidine Gluconate Gel
Lampe, M. F., Ballweber, L. M., Stamm, W. E.
To identify topical antimicrobial preparations which may be effective in preventing the transmission of sexually transmitted diseases, we examined the activity of chlorhexidine gluconate (CHG)...
Lampe, M. F., Ballweber, L. M., Isaacs, C. E., Patton, D. L., Stamm, W. E.
The development of new methods for prevention of sexually transmitted Chlamydia trachomatis infection is a top public health priority. Topical self-administered vaginal microbicides represent one...
Stapleton, A. E., Stroud, M. R., Hakomori, S. I., Stamm, W. E.
Women with a history of recurrent Escherichia coli urinary tract infections (UTIs) are significantly more likely to be nonsecretors of blood group antigens than are women without such a history, and...
Ballweber, L. M., Jaynes, J. E., Stamm, W. E., Lampe, M. F.
Topically applied microbicides that eradicate pathogens at the time of initial exposure represent a powerful strategy for the prevention of sexually transmitted infections. To aid in the further...
Ofloxacin versus trimethoprim-sulfamethoxazole for treatment of acute cystitis.
Hooton, T M, Latham, R H, Wong, E S, Johnson, C, Roberts, P L, Stamm, W E
We compared the safety and efficacies of ofloxacin and trimethoprim-sulfamethoxazole for the treatment of acute uncomplicated cystitis in women enrolled in a multicenter study. Data from three...
Characterization of the hemolytic activity of Haemophilus ducreyi.
Totten, P A, Norn, D V, Stamm, W E
H. ducreyi is the causative agent of chancroid, a genital ulcer disease most prevalent in developing countries. Chancroid enhances the heterosexual transmission of human immunodeficiency virus and is...
Lampe, M F, Wong, K G, Kuehl, L M, Stamm, W E
We have identified two families of novel Chlamydia trachomatis isolates with amino acid changes within the major outer membrane protein (MOMP) variable domains: one family of Da, D*, and D- and one...
Lichtenwalner, A B, Patton, D L, Cosgrove Sweeney, Y T, Gaur, L K, Stamm, W E
The macaque model of chlamydial pelvic inflammatory disease (PID) demonstrates individual variability in the time of onset of intrapelvic adhesions. Some animals develop adhesions rapidly, within 2...
Tartaglione, T A, Johnson, C R, Brust, P, Opheim, K, Hooton, T M, Stamm, W E
Vaginal colonization with Escherichia coli is an integral step in the development of acute cystitis, and persistent vaginal coliform colonization may also be a predisposing step to recurrent urinary...
Ineffectiveness and toxicity of BCG vaccine for the prevention of recurrent genital herpes.
Douglas, J M, Vontver, L A, Stamm, W E, Reeves, W C, Critchlow, C, Remington, M L, ...
One hundred fifty-five patients with genital herpes were enrolled in a double-blind, placebo-controlled trial comparing 0.1 ml of intradermal BCG vaccine with placebo for the prevention of recurrent...
The MICs of three new antimicrobial agents of different classes, U-70138F, roxithromycin (RU 965), and ofloxacin (ORF 18489), versus Chlamydia trachomatis in McCoy cell cultures were 0.25, 0.8, and...
Latham, R H, Zeleznik, D, Minshew, B H, Schoenknecht, F D, Stamm, W E
beta-Lactamase production and MIC determinations for penicillin, methicillin, and cephalothin were assessed for 67 strains of Staphylococcus saprophyticus and correlated with results of disk...
In vitro susceptibilities of Campylobacter-like organisms to twenty antimicrobial agents.
Flores, B M, Fennell, C L, Holmes, K K, Stamm, W E
We determined MICs of 20 antimicrobial agents for 50 representative strains of four subgroups of Campylobacter-like organisms (CLOs) by agar dilution. Ampicillin, gentamicin, doxycycline,...
Ofloxacin versus doxycycline for treatment of cervical infection with Chlamydia trachomatis.
Hooton, T M, Batteiger, B E, Judson, F N, Spruance, S L, Stamm, W E
Women with culture-proven Chlamydia trachomatis cervical infection were randomized to receive either ofloxacin (300 mg) or doxycycline (100 mg), orally twice daily for 7 days. All 56 had negative...
Direct antimicrobial susceptibility testing for acute urinary tract infections in women.
Johnson, J R, Tiu, F S, Stamm, W E
Despite its theoretical advantages, direct antimicrobial susceptibility testing (DST) of urine specimens remains controversial largely because of concerns regarding its accuracy, particularly with...
Suchland, K L, Counts, J M, Stamm, W E
The last decade has witnessed the development of a wide variety of diagnostic tests for Chlamydia trachomatis. In order to determine what laboratory methods are being used to detect C. trachomatis...
Hooton, T M, Johnson, C, Winter, C, Kuwamura, L, Rogers, M E, Roberts, P L, ...
We compared the safety and efficacy of a single 400-mg dose of ofloxacin, ofloxacin (200 mg) once daily for 3 days, and trimethoprim-sulfamethoxazole (160:800 mg) twice daily for 7 days for the...
Johnson, J R, Goullet, P, Picard, B, Moseley, S L, Roberts, P L, Stamm, W E
We determined the carboxylesterase B electrophoretic profiles of 74 blood isolates of Escherichia coli from patients with urosepsis. Most strains (64%) exhibited the B2 electrophoretic pattern. P...
McFarland, L V, Elmer, G W, Stamm, W E, Mulligan, M E
To determine whether strain-specific differences in immunoblot type, enterotoxin production, or cytotoxin production correlated with clinical presentation of Clostridium difficile infection, we...
Torian, B E, Reed, S L, Flores, B M, Creely, C M, Coward, J E, Vial, K, ...
A surface antigen (EH-96) of Entamoeba histolytica was demonstrated to be a plasma membrane antigen by immunoprecipitation of metabolically 35S-labeled antigen from live trophozoites, Triton X-114...
Johnson, J R, Moseley, S L, Roberts, P L, Stamm, W E
To assess the role of aerobactin as a virulence factor among uropathogenic Escherichia coli, we determined the prevalence, location, and phenotypic expression of aerobactin determinants among 58 E....
A Chlamydia trachomatis D* serovariant strain was found to be mixed with an F serovar strain in a clinical specimen. By using a monoclonal antibody which neutralizes serovar F infectivity in hamster...
Clear broth and plate media for culture of Haemophilus ducreyi.
Using catalase as a source of heme, we have developed both clear plate and broth media for the growth of Haemophilus ducreyi, the causative agent of chancroid. In the broth medium, the growth phase...
Ligase chain reaction to detect Chlamydia trachomatis infection of the cervix.
Schachter, J, Stamm, W E, Quinn, T C, Andrews, W W, Burczak, J D, Lee, H H
We performed a multicenter evaluation of ligase chain reaction (LCR) in the diagnosis of Chlamydia trachomatis infection of the cervix. This LCR provides an amplification of target sequences within...
"Campylobacter cinaedi" bacteremia: case report and laboratory findings.
Cimolai, N, Gill, M J, Jones, A, Flores, B, Stamm, W E, Laurie, W, ...
"Campylobacter cinaedi" was isolated from the blood of a 29-year-old homosexual man with previously diagnosed acquired immune deficiency syndrome. Subculturing of the organism was achieved with the...
Bauwens, J E, Clark, A M, Loeffelholz, M J, Herman, S A, Stamm, W E
To determine the accuracy of a recently developed polymerase chain reaction (PCR) urine assay to detect Chlamydia trachomatis urethral infection in men, we obtained urethral swabs and first-catch...
Bauwens, J E, Clark, A M, Stamm, W E
We evaluated a prototype polymerase chain reaction (PCR)-based assay for Chlamydia trachomatis developed by Roche Molecular Systems to detect endocervical infection in women. Of 587 endocervical...
Cles, L D, Bruch, K, Stamm, W E
Using purified elementary bodies of 14 Chlamydia trachomatis serovars in an in vitro assay, we compared the staining characteristics of six commercially available monoclonal antibody reagents used...
Campylobacter cinaedi is normal intestinal flora in hamsters.
Gebhart, C J, Fennell, C L, Murtaugh, M P, Stamm, W E
During the course of studies to reproduce proliferative enteritis in hamsters, Campylobacter cinaedi was recovered from the feces of the majority of healthy hamsters obtained from two commercial...
Use of HL cells for improved isolation and passage of Chlamydia pneumoniae.
We compared growth of the recently discovered respiratory pathogen Chlamydia pneumoniae in McCoy, HeLa 229, BHK-21, and HL cells. When cells were not pretreated with DEAE-dextran, HL cells had...
Flores, B M, Garcia, C A, Stamm, W E, Torian, B E
Monoclonal antibodies to Naegleria fowleri and Acanthamoeba polyphaga were analyzed by enzyme-linked immunosorbent assay, indirect immunofluorescence microscopy, and fluorescence flow cytometry to...
Schwebke, J R, Stamm, W E, Handsfield, H H
Endocervical infections due to Chlamydia trachomatis remain difficult to diagnose due to the lack of an inexpensive, rapid, and accurate test. We evaluated an alternative strategy for diagnosis in...
Barnes, R C, Wang, S P, Kuo, C C, Stamm, W E
The technical complexity of determining the serovar of Chlamydia trachomatis strains has limited the use of serotyping in clinical and epidemiologic studies. We developed a simple method for rapidly...
Isolation of "Campylobacter hyointestinalis" from a human.
Fennell, C L, Rompalo, A M, Totten, P A, Bruch, K L, Flores, B M, Stamm, W E
We isolated "Campylobacter hyointestinalis" from the rectal culture of a homosexual man with proctitis. Phenotypic characterization of the isolate was confirmed by DNA hybridization by using the...
Totten, P A, Patton, C M, Tenover, F C, Barrett, T J, Stamm, W E, Steigerwalt, A G, ...
A total of 593 strains of thermophilic Campylobacter species were isolated either from humans with diarrhea or from poultry in King County, Washington. Of these strains, 98 (52 hippurate-positive...
Ng, V L, Hadley, W K, Fennell, C L, Flores, B M, Stamm, W E
A bisexual human immunodeficiency virus-seropositive male had successive bacteremias with "Campylobacter cinaedi" and "Campylobacter fennelliae." Because final identification of both isolates was not...
Rectal swab cultures for Clostridium difficile surveillance studies.
McFarland, L V, Coyle, M B, Kremer, W H, Stamm, W E
We compared the recovery of Clostridium difficile from hospitalized patients by two collection methods: rectal swabs and stool cultures. Rectal swab cultures were as sensitive as stool cultures and...
Simplified microtiter cell culture method for rapid immunotyping of Chlamydia trachomatis.
Serotyping of Chlamydia trachomatis strains usually requires three to six serial passages in shell vials to attain sufficient antigen for typing procedures. To circumvent this problem, we developed a...
Use of a urine enzyme immunoassay as a diagnostic tool for Chlamydia trachomatis urethritis in men.
Schwebke, J R, Clark, A M, Pettinger, M B, Nsubga, P, Stamm, W E
We collected first-voided urine specimens from 659 males attending a sexually transmitted disease clinic and performed both enzyme immunoassay (EIA) for detection of chlamydial antigen and leukocyte...
Clark, A, Stamm, W E, Gaydos, C, Welsh, L, Quinn, T C, Schachter, J, ...
To evaluate the AntigEnz Chlamydia enzyme immunoassay (EIA) (Baxter/Bartels, Issaquah, Wash.), we studied 320 men and 1,209 women attending clinics for sexually transmitted diseases in Baltimore, San...
Antigen detection for the diagnosis of gonorrhea.
Stamm, W E, Cole, B, Fennell, C, Bonin, P, Armstrong, A S, Herrmann, J E, ...
We compared the diagnostic value of an enzyme immunoassay method for detection of gonococcal antigen in genital secretions with culture results and direct Gram stain for Neisseria gonorrhoeae in...
Stamm, W E, Tam, M, Koester, M, Cles, L
We compared two methods for identification of Chlamydia trachomatis inclusions in McCoy cell monolayers: conventional iodine staining and immunofluorescence staining with monoclonal antibodies...
Enzyme immunoassay for detection of antibody-coated bacteria.
Stamm, W E, Cutter, B E, Grootes-Reuvecamp, G A
To quantitatively evaluate factors potentially affecting antibody coating of bacteria in urine, we developed an assay with enzyme-linked rather than fluorescein-conjugated immunoglobulin. Using the...
Microtest procedure for isolation of Chlamydia trachomatis.
Yoder, B L, Stamm, W E, Koester, C M, Alexander, E R
Management of patients potentially infected with Chlamydia trachomatis has been hampered by the cost and time required to perform Chlamydia cultures. To isolate C. trachomatis, we developed a...
Lampe, M F, Suchland, R J, Stamm, W E
During studies in which we serotyped large numbers of Chlamydia trachomatis clinical isolates by using monoclonal antibodies, three novel serological variants (D-, D*, and I-) were identified. To...
Haemophilus ducreyi attaches to and invades human epithelial cells in vitro.
Totten, P A, Lara, J C, Norn, D V, Stamm, W E
Haemophilus ducreyi is a sexually transmitted pathogen that causes genital ulcers and inguinal adenopathy. Because chancroidal ulcers are most commonly located on the foreskins of uncircumcised...
Flores, B M, Fennell, C L, Kuller, L, Bronsdon, M A, Morton, W R, Stamm, W E
Campylobacter cinaedi and C. fennelliae have been associated with proctocolitis, bacteremia, and asymptomatic rectal infection, primarily in homosexual men. To more directly assess the pathogenic...
Stapleton, A, Nudelman, E, Clausen, H, Hakomori, S, Stamm, W E
Women with a history of recurrent Escherichia coli urinary tract infections (UTIs) are two to three times more likely to be nonsecretors of histo-blood group antigens than are women without such a...
Lampe, M. F., Rohan, L. C., Skinner, M. C., Stamm, W. E.
Commonly used “inactive” pharmaceutical excipients were tested in a previously developed minimum cidal concentration assay to assess their ability to kill Chlamydia trachomatis topically. Sixteen...
Sánchez, J, Gotuzzo, E, Escamilla, J, Carrillo, C, Phillips, I A, Barrios, C, ...
OBJECTIVES. This study examined the prevalences of antibodies to Treponema pallidum, Chlamydia trachomatis, and herpes simplex virus type 2 in a sample of Peruvian adults. METHODS. Among adults...
Serovar distribution of urogenital Chlamydia trachomatis strains in The Netherlands.
Wagenvoort, J H, Suchland, R J, Stamm, W E
The distribution of serovars in 208 Chlamydia trachomatis strains of urogenital origin isolated from 185 patients attending a sexually transmitted disease clinic in Rotterdam, The Netherlands, was...
Thorpe, E M, Stamm, W E, Hook, E W, Gall, S A, Jones, R B, Henry, K, ...
STUDY GOAL: To compare the efficacy and safety of single 1 g oral azithromycin with doxycycline, 100 mg twice daily for seven days for treatment of uncomplicated urogenital chlamydial infection....
Skinner, M. C., Stamm, W. E., Lampe, M. L.
A topical microbicide that women can use to prevent sexually transmitted diseases (STDs) is essential, and many microbicide candidates are being tested for activity against human immunodeficiency...