Garritsen, A, Van Galen, P J, Simonds, W F
We have identified the N terminus of the beta subunit as an essential domain for G-protein beta gamma assembly. A C-terminal fragment, beta 1-(130-340), fails to bind gamma unless coexpressed with...
Jones, T L, Simonds, W F, Merendino, J J, Brann, M R, Spiegel, A M
We transfected COS cells with cDNAs for the alpha subunits of stimulatory and inhibitory GTP-binding proteins, alpha s and alpha i1, respectively, and immunoprecipitated the metabolically labeled...
Simonds, W F, Goldsmith, P K, Codina, J, Unson, C G, Spiegel, A M
A panel of antibodies to synthetic decapeptides corresponding to the C termini of guanine nucleotide-binding regulatory protein (G protein) alpha subunits has been generated in rabbits. The...
Solubilization of active opiate receptors.
Simonds, W F, Koski, G, Streaty, R A, Hjelmeland, L M, Klee, W A
Receptors that reversibly bind opiates and opioid peptides have been solubilized from brain and neuroblastoma-glioma hybrid cell NG108-15 membranes. Active receptors are specifically solubilized with...
Purification of the opiate receptor of NG108-15 neuroblastoma-glioma hybrid cells.
Simonds, W F, Burke, T R, Rice, K C, Jacobson, A E, Klee, W A
Opiate receptors from NG108-15 neuroblastoma-glioma hybrid cell membranes were purified to apparent homogeneity in a form covalently labeled with the opiate affinity ligand...
Garritsen, A, Van Galen, P J, Simonds, W F
We have identified the N terminus of the beta subunit as an essential domain for G-protein beta gamma assembly. A C-terminal fragment, beta 1-(130-340), fails to bind gamma unless coexpressed with...
Jones, T L, Simonds, W F, Merendino, J J, Brann, M R, Spiegel, A M
We transfected COS cells with cDNAs for the alpha subunits of stimulatory and inhibitory GTP-binding proteins, alpha s and alpha i1, respectively, and immunoprecipitated the metabolically labeled...
Simonds, W F, Goldsmith, P K, Codina, J, Unson, C G, Spiegel, A M
A panel of antibodies to synthetic decapeptides corresponding to the C termini of guanine nucleotide-binding regulatory protein (G protein) alpha subunits has been generated in rabbits. The...
Solubilization of active opiate receptors.
Simonds, W F, Koski, G, Streaty, R A, Hjelmeland, L M, Klee, W A
Receptors that reversibly bind opiates and opioid peptides have been solubilized from brain and neuroblastoma-glioma hybrid cell NG108-15 membranes. Active receptors are specifically solubilized with...
Purification of the opiate receptor of NG108-15 neuroblastoma-glioma hybrid cells.
Simonds, W F, Burke, T R, Rice, K C, Jacobson, A E, Klee, W A
Opiate receptors from NG108-15 neuroblastoma-glioma hybrid cell membranes were purified to apparent homogeneity in a form covalently labeled with the opiate affinity ligand...