Khrapko, K., Hanekamp, J.S., Thilly, W.G., Belenkii, A., Foret, F., Karger, B.L.
Using a zone of constant temperature and denaturant concentration in capillary electrophoresis, we have devised a simple, rapid, and reproducible system for separating mutant from wild type DNA...
Cha, R S, Thilly, W G, Zarbl, H
GGA to GAA mutations in the 12th codon of the Hras gene are frequently observed in rat mammary tumors induced by N-nitroso-N-methylurea (NMU). We developed an assay to measure point mutations present...
An alkylation-tolerant, mutator human cell line is deficient in strand-specific mismatch repair.
Kat, A, Thilly, W G, Fang, W H, Longley, M J, Li, G M, Modrich, P
The human lymphoblastoid MT1 B-cell line was previously isolated as one of a series of mutant cells able to survive the cytotoxic effects of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). MT1 cells...
Mutational spectrometry: a general approach for hot-spot point mutations in selectable genes.
To observe point mutational spectra with a high degree of precision, independent large cultures of human lymphoblastoid cells were treated with a mutagen, benzo[a]pyrene diol epoxide, and mutants at...
Mutational spectrometry without phenotypic selection: human mitochondrial DNA.
Khrapko, K, Coller, H, André, P, Li, X C, Foret, F, Belenky, A, ...
By first separating mutant from nonmutant DNA sequences on the basis of their melting temperatures and then increasing the number of copies by high-fidelity DNA amplification, we have developed a...
Identification of point mutations in mixtures by capillary electrophoresis hybridization.
Khrapko, K, Coller, H A, Hanekamp, J S, Thilly, W G
We have developed a rapid method for unambiguous identification and mutant fraction determination of individual mutants in mixtures of DNA sequence variants each differing by one or a few...
Khrapko, K, Bodyak, N, Thilly, W G, Van Orsouw, N J, Zhang, X, Coller, H A, ...
Quantitative information on the cell-to-cell distribution of all possible mitochondrial DNA (mtDNA) mutations in young and aged tissues is needed to assess the relevance of these mutations to the...
Virus production with a newly developed microcarrier system.
Giard, D J, Thilly, W G, Wang, D I, Levine, D W
Primary cell cultures as well as established lines have been grown on a recently developed microcarrier configuration that overcomes the problem of toxicity attendant on earlier developments in this...
Fidelity of DNA polymerases in DNA amplification.
Denaturing gradient gel electrophoresis (DGGE) was used to separate and isolate the products of DNA amplification by polymerase chain reaction (PCR). The strategy permitted direct enumeration and...
Relative sensitivities of forward and reverse mutation assays in Salmonella typhimurium.
Skopek, T R, Liber, H L, Kaden, D A, Thilly, W G
Forward mutation to 8-azaguanine resistance and reverse mutation to histidine prototrophy were measured in Salmonella typhimurium after treatment with 16 mutagens of both base-substitution and...
Skopek, T R, Liber, H L, Krolewski, J J, Thilly, W G
We have developed a quantitative forward mutation assay using Salmonella typhimurium, in which resistance to the purine analog 8-azaguanine is used as a genetic marker. We present the assay protocol,...
Khrapko, K, Hanekamp, J S, Thilly, W G, Belenkii, A, Foret, F, Karger, B L
Using a zone of constant temperature and denaturant concentration in capillary electrophoresis, we have devised a simple, rapid, and reproducible system for separating mutant from wild type DNA...
Hannigan, M P, Cass, G R, Lafleur, A L, Busby, W F, Thilly, W G
The bacterial mutagenicity of a set of 1993 urban particulate air pollution samples is examined using the Salmonella typhimurium TM677 forward mutation assay. Amibent fine particulate samples were...
Rogers, C E, Tomita, A V, Trowbridge, P R, Gone, J K, Chen, J, Zeeb, P, ...
We hypothesized that residents of Woburn, Massachusetts, had been exposed to as much as 70 microg/l of arsenic (As) and 240 microg/l of chromium (Cr) in drinking water from municipal supply wells G...
Braun, A G, Busby, W F, Liber, H L, Thilly, W G
Extracts of effluents from a modern residential oil burner have been evaluated in several toxicological assay systems. Bacterial mutagens were detected in extracts from both the particulate and vapor...
Durant, J L, Chen, J, Hemond, H F, Thilly, W G
Between 1966 and 1986, the childhood leukemia rate in Woburn, Massachusetts, was 4-fold higher than the national average. A multidisciplinary research team from MIT, which is being supported by the...
A high-yield sampler for toxicological characterization of complex mixtures in combustion effluents.
Kruzel, E L, Lafleur, A L, Braun, A G, Longwell, J P, Thilly, W G, Peters, W A
Combustion sampling for toxicological assessment often requires that large (greater than 100 mg) lots of complex organic mixtures of wide volatility range be rapidly recovered from high temperature...
Determination of point mutational spectra of benzo[a]pyrene-diol epoxide in human cells.
The primary goal of our research consists of developing means sufficiently sensitive to allow assessment of human exposure to environmental carcinogens. We describe here a new approach for analyzing...
Lafleur, A L, Longwell, J P, Marr, J A, Monchamp, P A, Plummer, E F, Thilly, W G, ...
A number of isomeric C18H10 polycyclic aromatic hydrocarbons (PAHs), thought to be primarily cyclopenta-fused PAHs, are produced during the combustion and pyrolysis of fossil fuels. To determine the...
Bacterial mutagenicity of pyrolysis tars produced from chloro-organic fuels.
Mulholland, J A, Sarofim, A F, Longwell, J P, Lafleur, A L, Thilly, W G
Droplets of toluene and three chlorinated organics, ortho-dichlorobenzene, 1,2-dichloroethane, and trichloroethylene, were pyrolyzed in pure nitrogen. The composition and bacterial mutagenicity of...
A large number of hprt-mutants were obtained by treating human lymphoblast cells (TK6) with 5 microM K2Cr2O7 for 5 hr and selecting by growth in 6-thioguanine. A combination of high fidelity...
Assay of mutation induced in human lymphoblastoid cells by combustion-generated soot particles.
Bolsaitis, P P, Feitelberg, A S, Dekermendjian, V, Elliott, J F, Sarofim, A F, Thilly, W G
A human lymphoblastoid cell line has been used to test for mutations caused by combustion-generated soot particles and their constituent components, which are substrate carbon-black and adsorbed...
General approach to the biological analysis of complex mixtures.
Thilly, W G, Longwell, J, Andon, B M
The study of potential health effects of combustion effluents involves identifying the substances present and estimating the probable health hazards of each. Unfortunately, this second step cannot be...
Cha, R S, Thilly, W G, Zarbl, H
GGA to GAA mutations in the 12th codon of the Hras gene are frequently observed in rat mammary tumors induced by N-nitroso-N-methylurea (NMU). We developed an assay to measure point mutations present...
An alkylation-tolerant, mutator human cell line is deficient in strand-specific mismatch repair.
Kat, A, Thilly, W G, Fang, W H, Longley, M J, Li, G M, Modrich, P
The human lymphoblastoid MT1 B-cell line was previously isolated as one of a series of mutant cells able to survive the cytotoxic effects of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). MT1 cells...
Mutational spectrometry: a general approach for hot-spot point mutations in selectable genes.
To observe point mutational spectra with a high degree of precision, independent large cultures of human lymphoblastoid cells were treated with a mutagen, benzo[a]pyrene diol epoxide, and mutants at...
Mutational spectrometry without phenotypic selection: human mitochondrial DNA.
Khrapko, K, Coller, H, André, P, Li, X C, Foret, F, Belenky, A, ...
By first separating mutant from nonmutant DNA sequences on the basis of their melting temperatures and then increasing the number of copies by high-fidelity DNA amplification, we have developed a...
Identification of point mutations in mixtures by capillary electrophoresis hybridization.
Khrapko, K, Coller, H A, Hanekamp, J S, Thilly, W G
We have developed a rapid method for unambiguous identification and mutant fraction determination of individual mutants in mixtures of DNA sequence variants each differing by one or a few...
Khrapko, K, Bodyak, N, Thilly, W G, Van Orsouw, N J, Zhang, X, Coller, H A, ...
Quantitative information on the cell-to-cell distribution of all possible mitochondrial DNA (mtDNA) mutations in young and aged tissues is needed to assess the relevance of these mutations to the...
Virus production with a newly developed microcarrier system.
Giard, D J, Thilly, W G, Wang, D I, Levine, D W
Primary cell cultures as well as established lines have been grown on a recently developed microcarrier configuration that overcomes the problem of toxicity attendant on earlier developments in this...
Fidelity of DNA polymerases in DNA amplification.
Denaturing gradient gel electrophoresis (DGGE) was used to separate and isolate the products of DNA amplification by polymerase chain reaction (PCR). The strategy permitted direct enumeration and...
Relative sensitivities of forward and reverse mutation assays in Salmonella typhimurium.
Skopek, T R, Liber, H L, Kaden, D A, Thilly, W G
Forward mutation to 8-azaguanine resistance and reverse mutation to histidine prototrophy were measured in Salmonella typhimurium after treatment with 16 mutagens of both base-substitution and...
Skopek, T R, Liber, H L, Krolewski, J J, Thilly, W G
We have developed a quantitative forward mutation assay using Salmonella typhimurium, in which resistance to the purine analog 8-azaguanine is used as a genetic marker. We present the assay protocol,...
Khrapko, K, Hanekamp, J S, Thilly, W G, Belenkii, A, Foret, F, Karger, B L
Using a zone of constant temperature and denaturant concentration in capillary electrophoresis, we have devised a simple, rapid, and reproducible system for separating mutant from wild type DNA...
Hannigan, M P, Cass, G R, Lafleur, A L, Busby, W F, Thilly, W G
The bacterial mutagenicity of a set of 1993 urban particulate air pollution samples is examined using the Salmonella typhimurium TM677 forward mutation assay. Amibent fine particulate samples were...
Rogers, C E, Tomita, A V, Trowbridge, P R, Gone, J K, Chen, J, Zeeb, P, ...
We hypothesized that residents of Woburn, Massachusetts, had been exposed to as much as 70 microg/l of arsenic (As) and 240 microg/l of chromium (Cr) in drinking water from municipal supply wells G...
Braun, A G, Busby, W F, Liber, H L, Thilly, W G
Extracts of effluents from a modern residential oil burner have been evaluated in several toxicological assay systems. Bacterial mutagens were detected in extracts from both the particulate and vapor...
Durant, J L, Chen, J, Hemond, H F, Thilly, W G
Between 1966 and 1986, the childhood leukemia rate in Woburn, Massachusetts, was 4-fold higher than the national average. A multidisciplinary research team from MIT, which is being supported by the...
A high-yield sampler for toxicological characterization of complex mixtures in combustion effluents.
Kruzel, E L, Lafleur, A L, Braun, A G, Longwell, J P, Thilly, W G, Peters, W A
Combustion sampling for toxicological assessment often requires that large (greater than 100 mg) lots of complex organic mixtures of wide volatility range be rapidly recovered from high temperature...
Determination of point mutational spectra of benzo[a]pyrene-diol epoxide in human cells.
The primary goal of our research consists of developing means sufficiently sensitive to allow assessment of human exposure to environmental carcinogens. We describe here a new approach for analyzing...
Lafleur, A L, Longwell, J P, Marr, J A, Monchamp, P A, Plummer, E F, Thilly, W G, ...
A number of isomeric C18H10 polycyclic aromatic hydrocarbons (PAHs), thought to be primarily cyclopenta-fused PAHs, are produced during the combustion and pyrolysis of fossil fuels. To determine the...
Bacterial mutagenicity of pyrolysis tars produced from chloro-organic fuels.
Mulholland, J A, Sarofim, A F, Longwell, J P, Lafleur, A L, Thilly, W G
Droplets of toluene and three chlorinated organics, ortho-dichlorobenzene, 1,2-dichloroethane, and trichloroethylene, were pyrolyzed in pure nitrogen. The composition and bacterial mutagenicity of...
A large number of hprt-mutants were obtained by treating human lymphoblast cells (TK6) with 5 microM K2Cr2O7 for 5 hr and selecting by growth in 6-thioguanine. A combination of high fidelity...
Assay of mutation induced in human lymphoblastoid cells by combustion-generated soot particles.
Bolsaitis, P P, Feitelberg, A S, Dekermendjian, V, Elliott, J F, Sarofim, A F, Thilly, W G
A human lymphoblastoid cell line has been used to test for mutations caused by combustion-generated soot particles and their constituent components, which are substrate carbon-black and adsorbed...
General approach to the biological analysis of complex mixtures.
Thilly, W G, Longwell, J, Andon, B M
The study of potential health effects of combustion effluents involves identifying the substances present and estimating the probable health hazards of each. Unfortunately, this second step cannot be...
Cariello, N F, Scott, J K, Kat, A G, Thilly, W G, Keohavong, P
The combination of denaturing gradient gel electrophoresis (DGGE) and in vitro DNA amplification has allowed us to (1) localize a DNA mutation to a given 100-bp region of the human genome and (2)...