W. Hillen

Publication List Details

Period

1979 - 2000

Number

153

Co-Authors

Evaluation of Two- and Three-Tier Database Connections for a Java Based Medical Image Viewer (2000)

F. Unglauben, W. Hillen, M. Murdfield, Medizinische Informatik

. Different methods to connect a Java-Applet for Medical Image Visualisation to databases are presented. Using Java Database Connectivity (JDBC) two-tier and three-tier applications have been...

e + e − annihilation at high energies and search for the t -quark continuum contribution (1980)

Lynch, H. L., Hilger, E., Rimkus, J., Foster, B., Revel, D., Kadansky, V., ...

Measurements of R , sphericity and thrust are presented for c.m. energies between 12 and 31.6 GeV. A possible contribution of a t continuum can be ruled out for c.m. energies between 16 and 31 GeV.

Tetracycline-inducible expression systems with reduced basal activity in mammalian cells.

Forster, K, Helbl, V, Lederer, T, Urlinger, S, Wittenburg, N, Hillen, W

We describe a modification of the tetracycline-inducible eukaryotic gene expression system with decreased basal levels of expression in HeLa cells. It employs the tetracycline-inducible...

Nomenclature for tetracycline resistance determinants.

Levy, S B, McMurry, L M, Burdett, V, Courvalin, P, Hillen, W, Roberts, M C, ...

Tetracycline resistance determinants are widespread and distinguishable genetically and biochemically. The nomenclature for this increasing number of determinants has been varied and inconsistent....

Glucose and glucose-6-phosphate interaction with Xyl repressor proteins from Bacillus spp. may contribute to regulation of xylose utilization.

Dahl, M K, Schmiedel, D, Hillen, W

The xyl operons of several gram-positive bacteria are regulated at the level of transcription by xylose-responsive repressor proteins (XylR). In addition, they are catabolite repressed. Here, we...

Catabolite repression resistance of gnt operon expression in Bacillus subtilis conferred by mutation of His-15, the site of phosphoenolpyruvate-dependent phosphorylation of the phosphocarrier protein HPr.

Reizer, J, Bergstedt, U, Galinier, A, Küster, E, Saier, M H, Hillen, W, ...

Carbon catabolite repression of the gnt operon of Bacillus subtilis is mediated by the catabolite control protein CcpA and by HPr, a phosphocarrier protein of the phosphotransferase system....

Expression, inducer spectrum, domain structure, and function of MopR, the regulator of phenol degradation in Acinetobacter calcoaceticus NCIB8250.

Schirmer, F, Ehrt, S, Hillen, W

Degradation of phenol by Acinetobacter calcoaceticus NCIB8250 involves (sigma54-dependent expression of a multicomponent phenol hydroxylase and catechol 1,2-dioxygenase encoded by the mop operon....

Identification and characterization of xcpR encoding a subunit of the general secretory pathway necessary for dodecane degradation in Acinetobacter calcoaceticus ADP1.

Parche, S, Geissdörfer, W, Hillen, W

A mutant of Acinetobacter calcoaceticus ADP1 unable to grow on alkanes was complemented for growth on hexadecane with a DNA fragment encoding a protein with homology to XcpR, a subunit of the general...

Contribution of glucose kinase to glucose repression of xylose utilization in Bacillus megaterium.

Späth, C, Kraus, A, Hillen, W

The glk gene from Bacillus megaterium, which encodes glucose kinase, was isolated and analyzed. Disruption by a transcriptional glk-luxAB fusion indicated that glk is the only glucose kinase gene in...

Tetracycline repressor-regulated gene repression in recombinant human cytomegalovirus.

Kim, H J, Gatz, C, Hillen, W, Jones, T R

The tetracycline repressor (TetR)-regulated gene expression system from Escherichia coli was used to control gene expression in recombinant human cytomegalovirus (HCMV). To adapt the TetR system in...

Noninducible Tet repressor mutations map from the operator binding motif to the C terminus.

Hecht, B, Müller, G, Hillen, W

We have developed a new genetic selection system for Tet repressor mutations with a noninducible phenotype for tetracycline (TetRs). Extensive chemical mutagenesis of tetR yielded 93 single-site Tet...

Catabolite repression of the Bacillus subtilis xyl operon involves a cis element functional in the context of an unrelated sequence, and glucose exerts additional xylR-dependent repression.

Kraus, A, Hueck, C, Gärtner, D, Hillen, W

Catabolite repression (CR) of xylose utilization by Bacillus subtilis involves a 14-bp cis-acting element (CRE) located in the translated region of the gene encoding xylose isomerase (xylA)....

RpoN (sigma 54) is required for conversion of phenol to catechol in Acinetobacter calcoaceticus.

Ehrt, S, Ornston, L N, Hillen, W

Members of the sigma 54 protein family, encoded by rpoN, are required for the transcription of genes associated with specialized metabolic functions. The ability to grow with phenol appears to be a...

The Tn10-encoded tetracycline resistance mRNA contains a translational silencer in the 5' nontranslated region.

Flache, P, Baumeister, R, Hillen, W

We performed a mutational analysis of the left half of Tn10-encoded tet operator O2, located in the 5' nontranslated region of the mRNA for the resistance protein TetA, and determined the importance...

Regulation of Staphylococcus xylosus xylose utilization genes at the molecular level.

Sizemore, C, Wieland, B, Götz, F, Hillen, W

We have investigated the regulation of the operon encoding xylose utilization in Staphylococcus xylosus C2a and Staphylococcus carnosus TM300. For in vivo studies, transcriptional fusions of the...

Catabolite repression of the xyl operon in Bacillus megaterium.

Rygus, T, Hillen, W

We characterized catabolite repression of the genes encoding xylose utilization in Bacillus megaterium. A transcriptional fusion of xylA encoding xylose isomerase to the spoVG-lacZ indicator gene on...

Identification and sequence analysis of the Bacillus subtilis W23 xylR gene and xyl operator.

Kreuzer, P, Gärtner, D, Allmansberger, R, Hillen, W

The xyl operator of Bacillus subtilis W23 was identified by deletion analysis of the xyl regulatory region as a 25-base-pair (bp) sequence located 10 bp downstream from the xyl promoter. The outer 10...

Expression of the Bacillus subtilis xyl operon is repressed at the level of transcription and is induced by xylose.

Gärtner, D, Geissendörfer, M, Hillen, W

Expression of xylose isomerase was repressed in Bacillus subtilis strains W23, 168, and BR151 and could be induced in the presence of xylose. The expression was also glucose repressed in strains 168...

Constitutive expression of tetracycline resistance mediated by a Tn10-like element in Haemophilus parainfluenzae results from a mutation in the repressor gene.

Heuer, C, Hickman, R K, Curiale, M S, Hillen, W, Levy, S B

The Tn10-like constitutively expressed tetracycline resistance determinant from a Haemophilus parainfluenzae strain was cloned in Escherichia coli. Toxicity resulting from expression on multicopy...

Cloning and expression of the Acinetobacter calcoaceticus mutarotase gene in Escherichia coli.

Gatz, C, Altschmied, J, Hillen, W

This article describes the cloning of the mutarotase gene from Acinetobacter calcoaceticus and its expression in Escherichia coli. Purification of mutarotase (EC 5.1.3.3) led to a single polypeptide...

Transposon Tn10 contains two structural genes with opposite polarity between tetA and IS10R.

Schollmeier, K, Hillen, W

The nucleotide sequence of the central part of Tn10 has been determined from the rightmost HindIII site to IS10R. This sequence contains two open reading frames with opposite polarity. The in vivo...

Heterologous repressor-operator recognition among four classes of tetracycline resistance determinants.

Klock, G, Unger, B, Gatz, C, Hillen, W, Altenbuchner, J, Schmid, K, ...

Homologous and heterologous repressor-operator interactions among four different classes of tetracycline resistance determinants have been compared. These are represented by RP1/Tn1721 (class A),...

Structural requirements of tetracycline-Tet repressor interaction: determination of equilibrium binding constants for tetracycline analogs with the Tet repressor.

Degenkolb, J, Takahashi, M, Ellestad, G A, Hillen, W

We used the Tn10-encoded Tet repressor, which has a highly specific binding capacity for tetracycline, to probe contacts between the drug and protein by chemical interference studies of the...

Oligo[d(C).(G)] runs exhibit a helical repeat of 11.1 bp in solution and cause slight DNA curvature when properly phased.

Biburger, M, Niederweis, M, Hillen, W

We have inserted d(C)10 in a set of DNA fragments with bent segments on both ends, which are rotated with respect to each other by base pair wise increasing insertions. The electrophoretic mobilities...

Tet repressor binding induced curvature of tet operator DNA.

Tovar, K, Hillen, W

Tet repressor dimer binds to two tet operator sites spaced by 30 bp in the Tn10 encoded tet regulatory DNA. The effect of repressor binding on the gel mobility of circular permutated DNA fragments...

TET repressor.tet operator complex formation induces conformational changes in the tet operator DNA.

Altschmied, L, Hillen, W

The structural changes of the tet operator DNA upon binding of the TET repressor protein are examined by circular di