Amero, S A, Raychaudhuri, G, Cass, C L, Van Venrooij, W J, Habets, W J, Krainer, A R, ...
The major nuclear ribonucleoproteins (RNPs) involved in pre-mRNA processing are classified in broad terms either as small nuclear RNPs (snRNPs), which are major participants in the splicing reaction,...
Janssen, D B, Habets, W J, Marugg, J T, Van Der Drift, C
Mutants were isolated from Pseudomonas aeruginosa that were impaired in the utilization of a number of nitrogen sources. In contrast to the wild-type strain, these mutants appeared to be unable to...
Habets, W J, Sillekens, P T, Hoet, M H, McAllister, G, Lerner, M R, Van Venrooij, W J
Autoantibodies from a patient with systemic lupus erythematosus, which recognize U1 and U2 small nuclear ribonucleoprotein particles (snRNPs), were used to map B-cell autoepitopes on the U1...
Habets, W J, Sillekens, P T, Hoet, M H, Schalken, J A, Roebroek, A J, Leunissen, J A, ...
A U2 small nuclear RNA-associated protein, designated B'', was recently identified as the target antigen for autoimmune sera from certain patients with systemic lupus erythematosus and other...
Molecular cloning of the cDNA for the human U2 snRNA-specific A' protein.
Sillekens, P T, Beijer, R P, Habets, W J, Van Verooij, W J
The A' polypeptide is one of the protein constituents of the U2 snRNP particle. A potentially full-length cDNA clone containing the complete coding sequence for this U2 snRNP-specific protein was...
Zinc finger-like structure in U1-specific protein C is essential for specific binding to U1 snRNP.
Nelissen, R L, Heinrichs, V, Habets, W J, Simons, F, Lührmann, R, Van Venrooij, W J
The U1 small nuclear ribonucleoprotein (snRNP) contains three specific proteins denoted 70K, A and C, in addition to the common proteins. Specific functions of these proteins are not known although...
Sillekens, P T, Beijer, R P, Habets, W J, Van Venrooij, W J
A complementary DNA clone for the human U1 snRNP-specific C protein has been isolated. The nucleotide sequence of the 733 bp cDNA insert includes a 15 bp 5'-untranslated region, an open reading frame...
Scl-86, a marker antigen for diffuse scleroderma.
Van Venrooij, W J, Stapel, S O, Houben, H, Habets, W J, Kallenberg, C G, Penner, E, ...
More than 300 sera from patients with a connective tissue disease were analyzed with the immunoblotting technique. The presence of autoantibodies against an 86,000-mol wt marker antigen for diffuse...
Sillekens, P T, Habets, W J, Beijer, R P, Van Venrooij, W J
Sera from patients with connective tissue diseases often contain antibodies against snRNA-associated proteins. Using one of these sera in an immunological screening of a human lambda gt11 expression...
Characterization of the SS-B (La) antigen in adenovirus-infected and uninfected HeLa cells.
Habets, W J, Den Brok, J H, Boerbooms, A M, Van De Putte, L B, Van Venrooij, W J
The molecular composition and subcellular localization of the antigens recognized by anti-SS-B (La or Ha) antibodies was investigated. Ten anti-SS-B sera were selected by indirect immunofluorescence...
Mattaj, I W, Habets, W J, Van Venrooij, W J
Monospecific antibodies directed against several U small nuclear ribonucleoprotein (U snRNP) particle proteins were affinity purified from a patient's anti-(U1,U2)RNP serum. These were used to...
Amero, S A, Raychaudhuri, G, Cass, C L, Van Venrooij, W J, Habets, W J, Krainer, A R, ...
The major nuclear ribonucleoproteins (RNPs) involved in pre-mRNA processing are classified in broad terms either as small nuclear RNPs (snRNPs), which are major participants in the splicing reaction,...
Janssen, D B, Habets, W J, Marugg, J T, Van Der Drift, C
Mutants were isolated from Pseudomonas aeruginosa that were impaired in the utilization of a number of nitrogen sources. In contrast to the wild-type strain, these mutants appeared to be unable to...
Habets, W J, Sillekens, P T, Hoet, M H, McAllister, G, Lerner, M R, Van Venrooij, W J
Autoantibodies from a patient with systemic lupus erythematosus, which recognize U1 and U2 small nuclear ribonucleoprotein particles (snRNPs), were used to map B-cell autoepitopes on the U1...
Habets, W J, Sillekens, P T, Hoet, M H, Schalken, J A, Roebroek, A J, Leunissen, J A, ...
A U2 small nuclear RNA-associated protein, designated B'', was recently identified as the target antigen for autoimmune sera from certain patients with systemic lupus erythematosus and other...
Molecular cloning of the cDNA for the human U2 snRNA-specific A' protein.
Sillekens, P T, Beijer, R P, Habets, W J, Van Verooij, W J
The A' polypeptide is one of the protein constituents of the U2 snRNP particle. A potentially full-length cDNA clone containing the complete coding sequence for this U2 snRNP-specific protein was...
Zinc finger-like structure in U1-specific protein C is essential for specific binding to U1 snRNP.
Nelissen, R L, Heinrichs, V, Habets, W J, Simons, F, Lührmann, R, Van Venrooij, W J
The U1 small nuclear ribonucleoprotein (snRNP) contains three specific proteins denoted 70K, A and C, in addition to the common proteins. Specific functions of these proteins are not known although...
Sillekens, P T, Beijer, R P, Habets, W J, Van Venrooij, W J
A complementary DNA clone for the human U1 snRNP-specific C protein has been isolated. The nucleotide sequence of the 733 bp cDNA insert includes a 15 bp 5'-untranslated region, an open reading frame...
Scl-86, a marker antigen for diffuse scleroderma.
Van Venrooij, W J, Stapel, S O, Houben, H, Habets, W J, Kallenberg, C G, Penner, E, ...
More than 300 sera from patients with a connective tissue disease were analyzed with the immunoblotting technique. The presence of autoantibodies against an 86,000-mol wt marker antigen for diffuse...
Sillekens, P T, Habets, W J, Beijer, R P, Van Venrooij, W J
Sera from patients with connective tissue diseases often contain antibodies against snRNA-associated proteins. Using one of these sera in an immunological screening of a human lambda gt11 expression...
Characterization of the SS-B (La) antigen in adenovirus-infected and uninfected HeLa cells.
Habets, W J, Den Brok, J H, Boerbooms, A M, Van De Putte, L B, Van Venrooij, W J
The molecular composition and subcellular localization of the antigens recognized by anti-SS-B (La or Ha) antibodies was investigated. Ten anti-SS-B sera were selected by indirect immunofluorescence...
Mattaj, I W, Habets, W J, Van Venrooij, W J
Monospecific antibodies directed against several U small nuclear ribonucleoprotein (U snRNP) particle proteins were affinity purified from a patient's anti-(U1,U2)RNP serum. These were used to...
De Rooij, D J, Habets, W J, Van De Putte, L B, Hoet, M H, Verbeek, A L, Van Venrooij, W J
In a three year prospective study disease activity variables and levels of antibody against the RNP-peptides 70K and A were measured in 18 patients with mixed connective tissue disease. Antibody...
Verheijen, R, Van Den Hoogen, F, Beijer, R, Richter, A, Penner, E, Habets, W J, ...
We report the expression of a cDNA clone encoding 695 carboxyl-terminal amino acids of human DNA topoisomerase I (topoI) in Escherichia coli. More than 96% of the anti-HeLa topoI-positive sera from...
Habets, W J, De Rooij, D J, Salden, M H, Verhagen, A P, Van Eekelen, C A, Van De Putte, L B, ...
Specific nuclear proteins, separated according to their molecular weight (mol. wt) by polyacrylamide gel electrophoresis (PAGE) and subsequently transferred to nitrocellulose sheets, are able to bind...
Habets, W J, Hoet, M H, Sillekens, P T, De Rooij, D J, Van De Putte, L B, Van Venrooij, W J
A human cDNA expression library was screened with anti-ribonucleoprotein (RNP) antibodies from patients with connective tissue diseases. Three cDNA clones were isolated encoding 70 kD, A and B"...
Quantitation of anti-RNP and anti-Sm antibodies in MCTD and SLE patients by immunoblotting.
Habets, W J, De Rooij, D J, Hoet, M H, Van De Putte, L B, Van Venrooij, W J
A quantitative immunoblotting assay (QIBA) for the determination of specific antibody titres in human autoimmune sera is described. In this assay, a total HeLa nuclear protein fraction, immobilized...