Scientific Commons: W. J. Stiekema

Cross-species BAC-FISH painting of the tomato and potato chromosome 6 reveals undescribed chromosomal rearrangements (2008)

Tang, X., Szinay, D., Ramanna, M.S., Datema, E., Klein Lankhorst, R.M., ...

Ongoing genomics projects of tomato (Solanum lycopersicum ) and potato (Solanum tuberosum) are providing unique tools for comparative mapping studies in Solanaceae. At the chromosomal level, BACs can...

Local coexpression domains in the genome of rice show no microsynteny with Arabidopsis domains (2007)

Ren, X.Y., Stiekema, W.J., Nap, J.P.

Chromosomal coexpression domains are found in a number of different genomes under various developmental conditions. The size of these domains and the number of genes they contain vary. Here, we...

The CLAVATA3/ESR motif of CLAVATA3 is functionally independent from the nonconserved flanking sequences (2006)

Fiers, M.A., Golemiec, E., Li, K.W., Stiekema, W.J., ...

It is believed that CLAVATA3 (CLV3) encodes a peptide ligand that interacts with the CLV1/CLV2 receptor complex to limit the number of stem cells in the shoot apical meristem of Arabidopsis thaliana;...

In plants, highly expressed genes are least compact (2006)

Ren, X.Y., Vorst, O.F.J., Stiekema, W.J., Nap, J.P.H.

In both the monocot rice and the dicot Arabidopsis, highly expressed genes have more and longer introns and a larger primary transcript than genes expressed at a low level: higher expressed genes...

Local coexpression domains of two to four genes in the genome of Arabidopsis (2005)

Ren, X.Y., Stiekema, W.J., Nap, J.P.H.

Expression of genes in eukaryotic genomes is known to cluster, but cluster size is generally loosely defined and highly variable. We have here taken a very strict definition of cluster as sets of...

Chromosomal co-expression domains in the Arabidopsis thaliana genome (2004)

Ren, X.Y., Nap, J.P.H., Stiekema, W.J.

Genomics for Biosafety in Plant Biotechnology (2004)

Nap, J.P.H., Atanassov, A., Stiekema, W.J.

Bioinformatics for biosafety: predicting the potential allergenicity of GM food (2004)

Stiekema, W.J., Nap, J.P.H.

Chromosomal co-expression domains in the Arabidopsis thaliana genome (2004)

Ren, X.Y., Nap, J.P.H., Stiekema, W.J.

Complete genome sequence of Lactobacillus plantarum WCFS1 (2003)

Kleerebezem, M., Boekhorst, J., Molenaar, D., Kuipers, O.P., Leer, R., ...

The 3,308,274-bp sequence of the chromosome of Lactobacillus plantarum strain WCFS1, a single colony isolate of strain NCIMB8826 that was originally isolated from human saliva, has been determined,...

An ancient R gene from Solanum bulbocastanum confers broad-spectrum resistance to late Phytophthora infestans in cultivated potato and tomato (2003)

Sikkema, A., Gross, J., Stevens, P., Muskens, M., ...

Late blight, caused by the oomycete pathogen Phytophthora infestans, is the most devastating disease for potato cultivation. Here, we describe the positional cloning of the Rpi-blb1 gene from the...

Optimization of the Expression of Equistatin in Pichia pastoris (2002)

Outchkourov, N.S., Stiekema, W.J., Jongsma, M.A.

To improve the expression of equistatin, a proteinase inhibitor from the sea anemone Actinia equina, in the yeast Pichia pastoris, we prepared gene variants with yeast-preferred codon usage and lower...

Genomics and Sustainability : Exploring a Societal Norm (2002)

Nap, J.P., Jacobs, J., Gremmen, B., Stiekema, W.J.

Het genoom van de zandraket (2002)

Stiekema, W.J.

Genomics and sustainability : exploring a societal norm (2002)

Nap, J.P., Jacobs, J., Gremmen, B., Stiekema, W.J.

Induction of recessive mutations in potato using tissue culture techniques (2001)

Hoogkamp, T.J.H., Bergervoet, J.E.M., Visser, R.G.F., Jacobsen, E., Stiekema, W.J., ...

Development of Ac and Ds transposon tagging lines for gene isolation in diploid potato (2001)

Lasschuit, J., Stiekema, W.J., Jacobsen, E., Pereira, A.

For the development of an efficient transposon tagging strategy it is important to generate populations of plants containing unique independent transposon insertions that will mutate genes of...

Biology of Plant-Microbe Interactions - Vol. 2 (2000)

Bisseling, T., Stiekema, W.J.

Homologues of a single resistance-gene cluster in potato confer resistance to distinct pathogens: a virus and a nematode (2000)

Kanyuka, K., Bendahmane, A., Sandbrink, H., Baulcombe, D.C., ...

The isolation of the nematode-resistance gene Gpa2 in potato is described, and it is demonstrated that highly homologous resistance genes of a single resistance-gene cluster can confer resistance to...

Selection of independent Ds transposon insertions in somatic tissue of potato by protoplast regeneration (2000)

Bergervoet, J.E.M., Stiekema, W.J., Pereira, A., Jacobsen, E.

Potato is an autotetraploid crop plant that is not very amenable to the deployment of transposon tagging for gene cloning and gene identification. After diploidisation it is possible to get potato...

Potato Biotechnology: A global scenario (2000)

Jacobsen, E., Bachem, C.W.B., Pereira, A., Stiekema, W.J., Hoogkamp, T., ...

Target selected insertional mutagenesis on chromosome IV of Arabidopsis using the En-I transposon system (2000)

Speulman, E., Stiekema, W.J., Pereira, A.

Sandbrink, J.M., Colon, L.T., Hoogendoorn, C.J., Stiekema, W.J.

Two Paralogous Genes of the GPA2 resistance locus confer both virus and nematode resistance to potato (1999)

Stiekema, W.J., Sandbrink, H., Peters, S., Kanyuka, K., ...

Tight physical linkage of the nematode resistance gene Gpa2 and the virus resistance gene Rx on a single segment introgressed from the wild species Solanum tuberosum subsp. andigena CPC 1673 into cultivated potato (1999)

Kanyuka, K.A., Bendahmane, A., Stiekema, W.J., Klein Lankhorst, R., ...

Transgene-centred evaluation of genetically modified plants (1999)

Metz, P.L.J., Stiekema, W.J.

Bacillus thuringiensis toxin mediated insect resistance in plants (1999)

Bosch, D., Stiekema, W.J.

Gentechniek heeft ook voordelen (1999)

Stiekema, W.J., Jongsma, M., Bosch, D., Nap, J.P.

Matching Computers With Genetics. (1998)

Jansen, R.C., Sandbrink, H., Brandenburg, W., Stiekema, W.J.

Characterization and purification of a 40 kDa CryIC binding protein from cultured insect cells. (1998)

Kwa, M.S.G., Schipper, B., Stiekema, W.J., Vlak, J.M., Bosch, D.

Meiotic stability of transgene expression is unaffected by flanking matrix-associated regions (1998)

Conner, A.J., Mlynarova, L., Stiekema, W.J., Nap, J.P.

Evaluation of diallel analysis using ß-glucuronidase activity from transgenes in Nicotiana tabacum (1998)

Conner, A.J., Keizer, L.C.P., Mlynarova, L., Stiekema, W.J., Nap, J.P.

The cysteine protease activity of Colorado potato beetle (Leptinotarsa decemlineata Say) guts, which is insensitive to potato protease inhibitors, is inhibited by thyroglobulin type-I domain inhibitors (1998)

Gruden, K., Strukelj, B., Popovic, T., Lenarcic, B., Bevec, T., Brzin, J., ...

Biosafety of E. coli ß-glucuronidase (GUS) in plants (1998)

Gilissen, L.J.W., Metz, P.L.J., Stiekema, W.J., Nap, J.P.

Identification of R-gene homologous DNA fragments genetically linked to disease resistance loci in Arabidopsis thaliana (1998)

Aarts, M.G.M., Holub, E.B., Beynon, J.L., Stiekema, W.J., Pereira, A.

A transgene-centered approach to the biosafety of transgenic phosphinothricin-tolerant plants (1998)

Metz, P.L.J., Stiekema, W.J., Nap, J.P.

Unravelling the genome by genome sequencing & gene function analysis (1998)

Stiekema, W.J., Pereira, A.

The impact on biosafety of the phosphinothricin-tolerance transgene in inter-specific B. rapa X B. napus hybrids and their successive backcrosses. (1997)

Metz, P.L.J., Jacobsen, E., Nap, J.P., Pereira, A., Stiekema, W.J.

Improving scFv antibody expression levels in the plant cytosol. (1997)

Schouten, A., Roosien, J., Wilmink, A., Rosso, M.N., Bosch, D., ...

Expression of single-chain antibody fragments (scFvs) in the plant cytosol is often cumbersome. It was unexpectedly shown that addition at the C-terminus of the ER retention signal KDEL resulted in...

Towards plantibody-mediated resistance against nematodes. (1997)

Stiekema, W.J., Bosch, D., Wilmink, A., Schouten, A., Roosien, J., ...

Characterization of membrane proteins form cultured insect cells binding to the Bacillus thuringiensis cry1c toxin. (1997)

Kwa, M.S.G., Stiekema, W.J., Vlak, J.M., Bosch, D.

Improving scFv antibody levels in the plant cytocol (1997)

Schouten, A., Roosien, J., Wilmink, A., Rosso, M.N., Bosch, D., ...

Positional cloning of a gene for nematode resistance in sugar beet (1997)

Cai, D., Kleine, M., Kifle, S., Harloff, H.J., Sandal, N.N., Marcker, K.A., ...

Dissection of a synthesized quantitative trait to characterize transgene interactions (1997)

Nap, J.P.H., Conner, A.J., Mlynarova, L., Stiekema, W.J., Jansen, R.C.

Breeding for nematode resistance in sugar beet: A molecular approach (1997)

Kleine, M., Cai, D., Klein-Lankhorst, R., Sandal, N., Salentijn, E.M.J., Harloff, H., ...

The Arabidopsis MALE Sterility 2 protein shares similarity with reductases in elongation/condensation complexes (1997)

Aarts, M.G.M., Hodge, R., Kalantidis, K., Florack, D., Wilson, Z.A., Mulligan, B.J., ...

Backcrossing of nematode-resistant sugar beet: a second nematode resistance gene at the locus containing Hs1pro-1? (1997)

Sandal, N.N., Salentijn, E.M.J., Kleine, M., Cai, D., ...

Aspects of the biosafety of transgenic oilseed rape (Brassica napus Q (1997)

Metz, P.L.J., Jacobsen, E., Stiekema, W.J.

Occasional loss of expression of phosphinothricin tolerance in sexual offspring of transgenic oilseed rape (Brassica napus L.) (1997)

Metz, P.L.J., Jacobsen, E., Stiekema, W.J.

In defence of vertical resistance (1997)

Stiekema, W.J.

Mapping of resistance to the potato cyst nematode Globodera rostochiensis from the wild potato species Solanum vernei. (1996)

Jacobs, J.M.E., Horsman, K., Arens, P.F.P., Verkerk-Bakker, B., Jacobsen, E., ...

Race specific resistance againt Phytophthora infestans in potato is controlled by more genetic factors than only R-genes. (1996)

El-Kharbotly, A., Pereira, A., Stiekema, W.J., Jacobsen, E.

For RFLP mapping of R-genes, determining resistance to specific races of Phytophthora infestans in tetraploid potato, it is necessary to develop well segregating populations at the 2x level. During...

Localization of Ds-transposon containing T-DNA inserts in the diploid transgenic potato: linkage to the R1 resistance gene against Phytophthora infestans (Mont. de Bary). (1996)

El-Kharbotly, A., Jacobs, J.M.E., Jacobsen, E., Ramanna, M.S., Stiekema, W.J., ...

The C-terminal KDEL sequence increases the expression level of a single-chain antibody designed to be targeted to both the cytosol and the secretory pathway in transgenic tobacco. (1996)

Schouten, A., Roosien, J., Dejong, G.A.M., Zilverentant, J.F., ...

Plantibodies: calling in mouse genes to fight nematodew. (1996)

Roosien, J., Schouten, A., Overmars, H.A., Pomp, R., ...

(Pl)antibodies; a possible alternative in the absence of natural host-plant resistance genes. (1996)

Schouten, A., Roosien, J., Wilmink, A., ...

Domain III substitution in Bacillus thuringiensis delta-endotoxin CryIA(b) results in superior toxicity for Spodoptera exigua and altered membrane protein recognition. (1996)

Kwa, M.S.G., Yamamoto, T., Schipper, B., Vlak, J.M., ...

Toxicity of peptides to bacteria present in the vase water of cut roses (1996)

Florack, D.E.A., Stiekema, W.J., Bosch, D.

Erwinia soft rot resistance of potato cultivars expressing antimicrobial peptide tachyplesin 1 (1996)

Allefs, J.H.M., Florack, D.E.A., Hoogendoorn, J., Stiekema, W.J.

From transgene expression to public acceptance of transgenic plants: a matter of predictability (1996)

Nap, J.P., Mlynárová, L., Stiekema, W.J.

A good strategy for acceptable legislation of transgenic plants can be thought to be composed of several stacked levels of decision-making. These levels range from global to individual to cellular to...

Approaching the lower limits of transgene variability (1996)

Mlynárová, L., Keizer, L.P.C., Stiekema, W.J., Nap, J.P.

Characterisation and partial purification of gut cysteine and serine proteinases of Spodoptera exigua Hubner (Lepidoptera: noctuidae) (1996)

Jongsma, M.A., Peters, J., Bosch, D., Stiekema, W.J.

Combatting inhibitor-insensitive proteases of insect pests (1996)

Jongsma, M.A., Stiekema, W.J., Bosch, O.

Different domains of Bacillus thuringiensis delta-endotoxins can bind to insect midgut membrane proteins on ligand blots (1996)

Bakker, P.L., Stiekema, W.J., Bosch, D.

Combination of genetic maps of potato, using a new version of Joinmap. (1995)

Jacobs, J.M.E., Kreike, N.C.M., Jacobsen, E., Stiekema, W.J., Stam, P.

A genetic map of potato (Solanum tuberosum) integrating molecular markers, including transposons and classical markers. (1995)

Jacobs, J.M.E., Arens, P.F.P., Verkerk-Bakker, B., Bastiaanssen, H.J.M., ...