Scientific Commons: W. J. van Venrooij

The hU3-55K protein requires 15.5K binding to the box B/C motif as well as flanking RNA elements for its association with the U3 small nucleolar RNA in vitro (2002)

Granneman, S., Pruijn, G. J. M., Horstman, W., Van Venrooij, W. J., Luehrmann, R., Watkins, N. J.

The prognostic value of the antiperinuclear factor, anti-citrullinated peptide antibodies and rheumatoid factor in early rheumatoid arthritis (1999)

Ter Borg, E.J., Jacobs, J.W.G., Schellekens, G.A., ...

ObjectiveTo study the prognostic value of the antiperinuclear factor (APF), deter-mined by an indirect immunofluorescence test (IIF) and a recently developed anti-citrullinated cyclic pepide (CCP)...

The prognostic value of the antiperinuclear factor, anti-citrullinated peptide antibodies and rheumatoid factor in early rheumatoid arthritis (1999)

Ter Borg, E.J., Jacobs, J.W.G., Schellekens, G.A., ...

ObjectiveTo study the prognostic value of the antiperinuclear factor (APF), deter-mined by an indirect immunofluorescence test (IIF) and a recently developed anti-citrullinated cyclic pepide (CCP)...

Independent deposition of heterogeneous nuclear ribonucleoproteins and small nuclear ribonucleoprotein particles at sites of transcription.

Amero, S A, Raychaudhuri, G, Cass, C L, Van Venrooij, W J, Habets, W J, Krainer, A R, ...

The major nuclear ribonucleoproteins (RNPs) involved in pre-mRNA processing are classified in broad terms either as small nuclear RNPs (snRNPs), which are major participants in the splicing reaction,...

In vitro reconstitution of mammalian U1 snRNPs active in splicing: the U1-C protein enhances the formation of early (E) spliceosomal complexes.

Will, C L, Rümpler, S, Klein Gunnewiek, J, Van Venrooij, W J, Lührmann, R

We have established an in vitro reconstitution/splicing complementation system which has allowed the investigation of the role of mammalian U1 snRNP components both in splicing and at the early...

Characterization of the autoantigen La (SS-B) as a dsRNA unwinding enzyme.

Hühn, P, Pruijn, G J, Van Venrooij, W J, Bachmann, M

During the analysis of the La (SS-B) autoantigen for catalytic activities an ATP-dependent double-stranded RNA unwinding activity was detected. Both native and recombinant La proteins from different...

At least three linear regions but not the zinc-finger domain of U1C protein are exposed at the surface of the protein in solution and on the human spliceosomal U1 snRNP particle.

Dumortier, H, Klein Gunnewiek, J, Roussel, J P, Van Aarssen, Y, Briand, J P, Van Venrooij, W J, ...

No structural information on U1C protein either in its free state or bound to the spliceosomal U1 small nuclear ribonucleoprotein (snRNP) particle is currently available. Using rabbit antibodies...

Conserved features of Y RNAs revealed by automated phylogenetic secondary structure analysis.

Farris, A D, Koelsch, G, Pruijn, G J, Van Venrooij, W J, Harley, J B

Y RNAs are small 'cytoplasmic' RNAs which are components of the Ro ribonucleoprotein (RNP) complex. The core of this complex, which is found in the cell nuclei of higher eukaryotes as well as the...

hPop4: a new protein subunit of the human RNase MRP and RNase P ribonucleoprotein complexes.

Van Eenennaam, H, Pruijn, G J, Van Venrooij, W J

RNase MRP is a ribonucleoprotein particle involved in the processing of pre-rRNA. The RNase MRP particle is structurally highly related to the RNase P particle, which is involved in pre-tRNA...

Small nuclear RNA-associated proteins are immunologically related as revealed by mapping of autoimmune reactive B-cell epitopes.

Habets, W J, Sillekens, P T, Hoet, M H, McAllister, G, Lerner, M R, Van Venrooij, W J

Autoantibodies from a patient with systemic lupus erythematosus, which recognize U1 and U2 small nuclear ribonucleoprotein particles (snRNPs), were used to map B-cell autoepitopes on the U1...

Analysis of a cDNA clone expressing a human autoimmune antigen: full-length sequence of the U2 small nuclear RNA-associated B" antigen.

Habets, W J, Sillekens, P T, Hoet, M H, Schalken, J A, Roebroek, A J, Leunissen, J A, ...

A U2 small nuclear RNA-associated protein, designated B'', was recently identified as the target antigen for autoimmune sera from certain patients with systemic lupus erythematosus and other...

Homodimerization of the human U1 snRNP-specific protein C.

Gunnewiek, J M, Van Aarssen, Y, Wassenaar, R, Legrain, P, Van Venrooij, W J, Nelissen, R L

The U1 snRNP-specific protein C contains an N-terminal zinc finger-like CH motif which is required for the binding of the U1C protein to the U1 snRNP particle. Recently a similar motif was reported...

Common structural features of the Ro RNP associated hY1 and hY5 RNAs.

Van Gelder, C W, Thijssen, J P, Klaassen, E C, Sturchler, C, Krol, A, Van Venrooij, W J, ...

The secondary structures of human hY1 and hY5 RNAs were determined using both chemical modification techniques and enzymatic structure probing. The results indicate that both for hY1 and for hY5 RNA...

The La antigen inhibits the activation of the interferon-inducible protein kinase PKR by sequestering and unwinding double-stranded RNA.

Xiao, Q, Sharp, T V, Jeffrey, I W, James, M C, Pruijn, G J, Van Venrooij, W J, ...

The La (SS-B) autoimmune antigen is an RNA-binding protein that is present in both nucleus and cytoplasm of eukaryotic cells. The spectrum of RNAs that interact with the La antigen includes species...

Purification and characterization of human autoantibodies directed to specific regions on U1RNA; recognition of native U1RNP complexes.

Hoet, R M, Kastner, B, Lührmann, R, Van Venrooij, W J

Antibodies against naked U1RNA can be found in sera from patients with overlap syndromes of systemic lupus erythematosus (SLE) in addition to antibodies directed to the proteins of U1...

Topoisomerase I identified by scleroderma 70 antisera: enrichment of topoisomerase I at the centromere in mouse mitotic cells before anaphase.

Maul, G G, French, B T, Van Venrooij, W J, Jimenez, S A

Antibodies derived from scleroderma patients positive for the extractable 70-kDa antigen were shown to react with topoisomerase I. Topoisomerase I was identified by molecular size and by antibody...

Analysis of in vitro binding of U1-A protein mutants to U1 snRNA.

Boelens, W, Scherly, D, Jansen, E J, Kolen, K, Mattaj, I W, Van Venrooij, W J

Despite the great sequence similarity between U1A and U2B", both proteins do have a difference in RNA binding specificity and in the way they bind to their cognate RNAs. The U1A protein is able to...

Analysis of protein--RNA interactions within Ro ribonucleoprotein complexes.

Pruijn, G J, Slobbe, R L, Van Venrooij, W J

The interactions between Ro and La proteins and hY RNAs have been analysed. The binding site for the 60 kDa Ro protein on hY RNAs is shown to be the terminal part of the base paired stem structure,...

Anti-(U1) small nuclear RNA antibodies in anti-small nuclear ribonucleoprotein sera from patients with connective tissue diseases.

Van Venrooij, W J, Hoet, R, Castrop, J, Hageman, B, Mattaj, I W, Van De Putte, L B

Small nuclear ribonucleoprotein (snRNP) particles are a class of RNA-containing particles in the nucleus of eukaryotic cells. Sera from patients with connective tissue diseases often contain...

Zinc finger-like structure in U1-specific protein C is essential for specific binding to U1 snRNP.

Nelissen, R L, Heinrichs, V, Habets, W J, Simons, F, Lührmann, R, Van Venrooij, W J

The U1 small nuclear ribonucleoprotein (snRNP) contains three specific proteins denoted 70K, A and C, in addition to the common proteins. Specific functions of these proteins are not known although...

A weak interaction between the U2A' protein and U2 snRNA helps to stabilize their complex with the U2B" protein.

Boelens, W, Scherly, D, Beijer, R P, Jansen, E J, Dathan, N A, Mattaj, I W, ...

The U2 snRNP complex contains two specific proteins, U2B" and U2A'. We have analysed the interaction of U2A' with U2B" and with U2 RNA. U2A' can form an weak but detectable RNA-protein complex with...

Human U1 snRNP-specific C protein: complete cDNA and protein sequence and identification of a multigene family in mammals.

Sillekens, P T, Beijer, R P, Habets, W J, Van Venrooij, W J

A complementary DNA clone for the human U1 snRNP-specific C protein has been isolated. The nucleotide sequence of the 733 bp cDNA insert includes a 15 bp 5'-untranslated region, an open reading frame...

U1 small nuclear ribonucleoprotein particle-specific proteins interact with the first and second stem-loops of U1 RNA, with the A protein binding directly to the RNA independently of the 70K and Sm proteins.

Patton, J R, Habets, W, Van Venrooij, W J, Pederson, T

The U1 small nuclear ribonucleoprotein particle (U1 snRNP), a cofactor in pre-mRNA splicing, contains three proteins, termed 70K, A, and C, that are not present in the other spliceosome-associated...

The association of the U1-specific 70K and C proteins with U1 snRNPs is mediated in part by common U snRNP proteins.

Nelissen, R L, Will, C L, Van Venrooij, W J, Lührmann, R

The U1 small nuclear ribonucleoprotein particle (snRNP)-specific 70K and A proteins are known to bind directly to stem-loops of the U1 snRNA, whereas the U1-C protein does not bind to naked U1 snRNA,...

Identification of the RNA binding segment of human U1 A protein and definition of its binding site on U1 snRNA.

Scherly, D, Boelens, W, Van Venrooij, W J, Dathan, N A, Hamm, J, Mattaj, I W

The interaction between the U1 snRNP-specific U1 A protein and U1 snRNA has been analysed. The binding site for the protein on the RNA is shown to be in hairpin II, which extends from positions 48 to...

A complex secondary structure in U1A pre-mRNA that binds two molecules of U1A protein is required for regulation of polyadenylation.

Van Gelder, C W, Gunderson, S I, Jansen, E J, Boelens, W C, Polycarpou-Schwarz, M, Mattaj, I W, ...

The human U1A protein-U1A pre-mRNA complex and the relationship between its structure and function in inhibition of polyadenylation in vitro were investigated. Two molecules of U1A protein were shown...

Scl-86, a marker antigen for diffuse scleroderma.

Van Venrooij, W J, Stapel, S O, Houben, H, Habets, W J, Kallenberg, C G, Penner, E, ...

More than 300 sera from patients with a connective tissue disease were analyzed with the immunoblotting technique. The presence of autoantibodies against an 86,000-mol wt marker antigen for diffuse...

Epitope regions on U1 small nuclear RNA recognized by anti-U1RNA-specific autoantibodies.

Hoet, R M, De Weerd, P, Gunnewiek, J K, Koornneef, I, Van Venrooij, W J

Autoantibodies specifically directed to U1RNA were found in patients suffering from systemic lupus erythematosus (SLE) overlap syndromes. To obtain more insight in the mechanism responsible for this...

hPop1: an autoantigenic protein subunit shared by the human RNase P and RNase MRP ribonucleoproteins.

Lygerou, Z, Pluk, H, Van Venrooij, W J, Séraphin, B

The eukaryotic endonucleases RNase P and RNase MRP require both RNA and protein subunits for function. Even though the human RNase P and MRP RNAs were previously characterized, the protein...

Citrulline is an essential constituent of antigenic determinants recognized by rheumatoid arthritis-specific autoantibodies.

Schellekens, G A, De Jong, B A, Van Den Hoogen, F H, Van De Putte, L B, Van Venrooij, W J

Only a few autoantibodies that are more or less specific for RA have been described so far. The rheumatoid factor most often tested for is not very specific for RA, while the more specific...

The U2B'' RNP motif as a site of protein-protein interaction.

Scherly, D, Dathan, N A, Boelens, W, Van Venrooij, W J, Mattaj, I W

The U2 snRNP contains two specific proteins, U2B'' and U2A'. Neither of these proteins, on its own, is capable of specific interactions with U2 RNA. Here, a complex between U2B'' and U2A' that forms...

cDNA cloning of the human U1 snRNA-associated A protein: extensive homology between U1 and U2 snRNP-specific proteins.

Sillekens, P T, Habets, W J, Beijer, R P, Van Venrooij, W J

Sera from patients with connective tissue diseases often contain antibodies against snRNA-associated proteins. Using one of these sera in an immunological screening of a human lambda gt11 expression...

Characterization of the SS-B (La) antigen in adenovirus-infected and uninfected HeLa cells.

Habets, W J, Den Brok, J H, Boerbooms, A M, Van De Putte, L B, Van Venrooij, W J

The molecular composition and subcellular localization of the antigens recognized by anti-SS-B (La or Ha) antibodies was investigated. Ten anti-SS-B sera were selected by indirect immunofluorescence...

Monospecific antibodies reveal details of U2 snRNP structure and interaction between U1 and U2 snRNPs.

Mattaj, I W, Habets, W J, Van Venrooij, W J

Monospecific antibodies directed against several U small nuclear ribonucleoprotein (U snRNP) particle proteins were affinity purified from a patient's anti-(U1,U2)RNP serum. These were used to...

The interactions with Ro60 and La differentially affect nuclear export of hY1 RNA.

Simons, F H, Rutjes, S A, Van Venrooij, W J, Pruijn, G J

Ro RNPs are evolutionarily conserved ribonucleoprotein particles that consist of a small RNA, known as Y RNA, associated with several proteins, such as La, Ro60, and Ro52. The Y RNAs (Y1-Y5), which...

Characterization of an anti-RNA recombinant autoantibody fragment (scFv) isolated from a phage display library and detailed analysis of its binding site on U1 snRNA.

Teunissen, S W, Stassen, M H, Pruijn, G J, Van Venrooij, W J, Hoet, R M

This is the first study in which the complex of a monoclonal autoantibody fragment and its target, stem loop II of U1 snRNA, was investigated with enzymatic and chemical probing. A phage display...

RNA-protein interactions in the human RNase MRP ribonucleoprotein complex.

Pluk, H, Van Eenennaam, H, Rutjes, S A, Pruijn, G J, Van Venrooij, W J

The eukaryotic nucleolus contains a large number of small RNA molecules that, in the form of small nucleolar ribonucleoprotein complexes (snoRNPs), are involved in the processing and modification of...

Yeast Rrp9p is an evolutionarily conserved U3 snoRNP protein essential for early pre-rRNA processing cleavages and requires box C for its association.

Venema, J, Vos, H R, Faber, A W, Van Venrooij, W J, Raué, H A

Pre-rRNA processing in eukaryotic cells requires participation of several snoRNPs. These include the highly conserved and abundant U3 snoRNP, which is essential for synthesis of 18S rRNA. Here we...

Identification of a novel cis-acting RNA element involved in nuclear export of hY RNAs.

Rutjes, S A, Lund, E, Grimm, C, Van Venrooij, W J, Pruijn, G J

Ro RNPs are small cytoplasmic RNA-protein complexes of unknown function that have been found in all metazoan cells studied so far. In human cells, Ro RNPs consist of one of four small RNA molecules,...