Scientific Commons: W. Ostertag

Dimethylsulfoxide-induced differentiation and hemoglobin synthesis in tissue cultures of rat erythroleukemia cells transformed by 7,12-dimethylbenz(a)anthracene (1976)

Kluge, N., Ostertag, W., Sugiyama, T., Arndt-Jovin, D., Steinheider, G., Furusawa, M., ...

Permanent cell lines from transplantable tumors from 7,12-dimethylbenz(a)anthracene-induced erythroleukemia of the rat were established. These cell lines maintain their erythroid nature. Erythroid...

Induction of endogenous and of spleen focus-forming viruses during dimethylsulfoxide-induced differentiation of mouse erythroleukemia cells transformed by spleen focus-forming virus (1975)

Dube, S. K., Pragnell, I. B., Kluge, N., Gaedicke, G., Steinheider, G., Ostertag, W.

Embryonic stem cell virus, a recombinant murine retrovirus with expression in embryonic stem cells.

Grez, M, Akgün, E, Hilberg, F, Ostertag, W

The expression of Moloney murine leukemia virus and vectors derived from it is restricted in undifferentiated mouse embryonal carcinoma and embryonal stem (ES) cells. We have developed a retroviral...

Rapid and efficient cloning of proviral flanking fragments by kanamycin resistance gene complementation.

Fehse, B, Kühlcke, K, Langer, A, Ostertag, W, Lother, H

We have developed a technique for the rapid cloning of unknown flanking regions of transgenic DNA. We complemented a truncated kanamycin resistance gene of a bacterial plasmid with a neomycin...

Inheritance of susceptibility to the myeloproliferative sarcoma virus: effect of the Fv-2 locus and evidence for a myeloproliferative sarcoma virus resistance locus.

Ostertag, W, Odaka, T, Smadja-Joffe, F, Jasmin, C

Myeloproliferative sarcoma virus (MPSV) causes a generalized stem cell leukemia with erythroid and myeloid hyperplasia in adult mice. MPSV also transforms fibroblasts. Mice congenic for the Fv-2...

Analysis of the myeloproliferative sarcoma virus genome: limited changes in the prototype lead to altered target cell specificity.

Pragnell, I B, Fusco, A, Arbuthnott, C, Smadja-Joffe, F, Klein, B, Jasmin, C, ...

The myeloproliferative sarcoma virus (MPSV) derived from Moloney sarcoma virus (MSV-Mol) is a unique sarcoma virus which causes expansion of the hematopoietic stem cell compartment as well as the...

Novel retroviral vectors for efficient expression of the multidrug resistance (mdr-1) gene in early hematopoietic cells.

Baum, C, Hegewisch-Becker, S, Eckert, H G, Stocking, C, Ostertag, W

We present data that retroviral gene expression in early hematopoietic cells is subjected to transcriptional controls similar to those previously described for embryonic stem cells. Transient...

The potent enhancer activity of the polycythemic strain of spleen focus-forming virus in hematopoietic cells is governed by a binding site for Sp1 in the upstream control region and by a unique enhancer core motif, creating an exclusive target for PEBP/CBF.

Baum, C, Itoh, K, Meyer, J, Laker, C, Ito, Y, Ostertag, W

The polycythemic strain of the spleen focus-forming virus (SFFVp) contains the most potent murine retroviral enhancer configuration known so far for gene expression in myeloerythroid hematopoietic...

Sequential mutations in the interleukin-3 (IL3)/granulocyte-macrophage colony-stimulating factor/IL5 receptor beta-subunit genes are necessary for the complete conversion to growth autonomy mediated by a truncated beta C subunit.

Hannemann, J, Hara, T, Kawai, M, Miyajima, A, Ostertag, W, Stocking, C

An amino-terminally truncated beta C receptor (beta C-R) subunit of the interleukin-3 (IL3)/granulocyte-macrophage colony-stimulating factor/IL5 receptor complex mediates factor-independent and...

Selectable retrovirus vectors encoding Friend virus gp55 or erythropoietin induce polycythemia with different phenotypic expression and disease progression.

Ahlers, N, Hunt, N, Just, U, Laker, C, Ostertag, W, Nowock, J

The Friend spleen focus-forming virus induces a massive expansion of erythroid progenitor cells resulting in polycythemia and splenomegaly. The pathogenic agent is the membrane glycoprotein gp55,...

v-mos proteins encoded by myeloproliferative sarcoma virus and its ts159 mutant.

Singh, B, Stocking, C, Walker, R, Yang, Y D, Ostertag, W, Arlinghaus, R B

The myeloproliferative sarcoma virus (MPSV) v-mos protein was predicted to be identical in size to p39c-mos because of an observed one-base deletion in the seventh codon of the env-mos open reading...

The malignant histiocytosis sarcoma virus, a recombinant of Harvey murine sarcoma virus and Friend mink cell focus-forming virus, has acquired myeloid transformation specificity by alterations in the long terminal repeat.

Friel, J, Hughes, D, Pragnell, I, Stocking, C, Laker, C, Nowock, J, ...

The malignant histiocytosis sarcoma virus (MHSV), in contrast to other viruses with the ras oncogene, induces acute histiocytosis in newborn and adult mice. Molecular structure and function studies...

A temperature-sensitive mutant of the myeloproliferative sarcoma virus, altered by a point mutation in the mos oncogene, has been modified as a selectable retroviral vector.

Friel, J, Stocking, C, Stacey, A, Ostertag, W

The myeloproliferative sarcoma virus (MPSV) is a mos-oncogenic retrovirus which induces an acute myeloproliferative disease in adult mice. The isolation and molecular cloning of two mutants of MPSV...

Action of temperature-sensitive mutants of myeloproliferative sarcoma virus suggests that fibroblast-transforming and hematopoietic transforming viral properties are related.

Ostertag, W, Freshney, M, Vehmeyer, K, Jasmin, C, Rutter, G

The myeloproliferative sarcoma virus is molecularly related to the Moloney sarcoma virus (Pragnell et al., J. Virol. 38:952-957, 1981), but causes both fibroblast transformation in vitro and leukemic...

Molecular cloning and characterization of a leukemia-inducing myeloproliferative sarcoma virus and two of its temperature-sensitive mutants.

Kollek, R, Stocking, C, Smadja-Joffe, F, Ostertag, W

The myeloproliferative sarcoma virus (MPSV) induces extensive hematopoietic changes, including spleen foci in adult mice, and transforms fibroblasts in vitro. NRK nonproducer cell lines of MPSV and...

Comparison of myeloproliferative sarcoma virus with Moloney murine sarcoma virus variants by nucleotide sequencing and heteroduplex analysis.

Stacey, A, Arbuthnott, C, Kollek, R, Coggins, L, Ostertag, W

The myeloproliferative sarcoma virus (MPSV) was derived by passage of Moloney sarcoma virus (Mo-MuSV) in adult mice. Mo-MuSV variants transform fibroblasts. However, MPSV also affects erythroid,...

Two distinct sequence elements mediate retroviral gene expression in embryonal carcinoma cells.

Weiher, H, Barklis, E, Ostertag, W, Jaenisch, R

Moloney murine leukemia virus (M-MuLV) and M-MuLV-derived retroviral vectors are not expressed in early mouse embryos or in embryonal carcinoma cells. M-MuLV-derived mutants or M-MuLV-related...

Myeloproliferative virus, a cloned murine sarcoma virus with spleen focus-forming properties in adult mice.

Ostertag, W, Vehmeyer, K, Fagg, B, Pragnell, I B, Paetz, W, Le Bousse, M C, ...

Myeloproliferative virus, derived from Moloney sarcoma virus, causes erythroleukemia and myeloid leukemia in adult mice. This virus is also capable of fibroblast transformation in vitro. The virus...

Functional analysis of a retroviral host-range mutant: altered long terminal repeat sequences allow expression in embryonal carcinoma cells.

Hilberg, F, Stocking, C, Ostertag, W, Grez, M

A retroviral host-range neomycin-resistant myeloproliferative sarcoma virus mutant, which is expressed in the embryonal carcinoma cell lines F9 and PCC4aza1R, was molecularly cloned and analyzed....

Autocrine stimulation after transfer of the granulocyte/macrophage colony-stimulating factor gene and autonomous growth are distinct but interdependent steps in the oncogenic pathway.

Laker, C, Stocking, C, Bergholz, U, Hess, N, De Lamarter, J F, Ostertag, W

Autocrine stimulation of cells by aberrant synthesis of growth factor may lead to malignant transformation, either as a direct consequence of endogenous factor production or as a first step of a...

Excision of specific DNA-sequences from integrated retroviral vectors via site-specific recombination.

Bergemann, J, Kühlcke, K, Fehse, B, Ratz, I, Ostertag, W, Lother, H

Vectors for gene transfer and gene therapy were developed which combine the advantages of the integrase and recombinase systems. This was achieved by inserting two loxP sites for specific DNA...

Erythroid differentiation and commitment in rat erythroleukemia cells with hypertonic culture conditions.

Yamaguchi, Y, Kluge, N, Ostertag, W, Furusawa, M

Cell cultures of 7,12-dimethylbenz[a]anthracene-induced rat erythroleukemia can be stimulated to synthesize hemoglobin when cultured in hypertonic media. During hypertonic treatment the intracellular...

Inducibility of spleen focus-forming virus by BrdUrd is controlled by the differentiated state of the cell.

Greiser-Wilke, I, Ostertag, W, Goldfarb, P, Lang, A, Furusawa, M, Conscience, J F

All Friend cells--except thymidine kinase (ATP:thymidine 5'-phosphotransferase, EC 2.7.1.21)-deficient mutants--are highly inducible for the release of biologically active spleen focus-forming virus...

Retroviral mutants efficiently expressed in embryonal carcinoma cells.

Franz, T, Hilberg, F, Seliger, B, Stocking, C, Ostertag, W

The myeloproliferative sarcoma virus (MPSV) is a unique member of the Moloney murine sarcoma virus family. Due to mutations in the U3 region of its long terminal repeat, MPSV has an expanded host...

Continuous suppression of globin gene expression and differentiation of Friend erythroleukemia cells by phorbol 12-myristate 13-acetate (PMA) despite the loss of PMA binding sites by down regulation.

Yamasaki, H, Martel, N, Fusco, A, Ostertag, W

The tumor promoter phorbol 12-myristate 13-acetate (PMA) reversibly inhibits hexamethylene bisacetamide-induced terminal differentiation of Friend erythroleukemia cells (FELC). We were successful in...

Size, subunit composition, and secondary structure of the Friend virus genome.

Dube, S, Kung, H J, Bender, W, Davidson, N, Ostertag, W

Electron microscope and gel electrophoresis studies show that the high-molecular-weight (50 to 70S) RNA extract from Friend virus (FV) is a dimer with the same basic structure previously observed for...

Differentiation arrest and stromal cell-independent growth of murine erythroleukemia cells are associated with elevated expression of ets-related genes but not with mutation of p53.

Nibbs, R J, Itoh, K, Ostertag, W, Harrison, P R

The ELM erythroleukemia is novel in that long-term survival of leukemic cells in culture (ELM-D cells) is dependent on contact with a bone marrow-derived stromal feeder cell layer. However, a number...

The chicken lysozyme 5' matrix attachment region increases transcription from a heterologous promoter in heterologous cells and dampens position effects on the expression of transfected genes.

Phi-Van, L, Von Kries, J P, Ostertag, W, Strätling, W H

Matrix attachment regions (MARs) are DNA elements that dissect the genome into topologically separated domains by binding to a chromosomal skeleton. This study explored the putative influence of the...

Rates of mutation to growth factor autonomy and tumorigenicity differ in hematopoietic stem and precursor cells expressing the multilineage colony-stimulating factor gene.

Laker, C, Kluge, N, Stocking, C, Just, U, Franz, M J, Ostertag, W, ...

At least two separate but interdependent events are required to attain autonomous growth as a consequence of ectopic expression of the multilineage colony-stimulating factor gene in hematopoietic...

Viral transfer, transcription, and rescue of a selectable myeloproliferative sarcoma virus in embryonal cell lines: expression of the mos oncogene.

Seliger, B, Kollek, R, Stocking, C, Franz, T, Ostertag, W

A derivative of the myeloproliferative sarcoma virus (Neor-MPSV) carrying the mos oncogene and dominant selection marker for neomycin resistance (Neor) was introduced into embryonal carcinoma and...

Lack of retrovirus gene expression in somatic cell hybrids of friend cells and teratocarcinoma cells with a teratocarcinoma phenotype.

Asche, W, Colletta, G, Warnecke, G, Nobis, P, Pennie, S, King, R M, ...

Two types of hybrids between cells with erythroid phenotype (Friend cells) and teratocarcinoma cells can be distinguished: cell hybrids with an erythroid phenotype, which release or can be induced to...

Long terminal repeat sequences impart hematopoietic transformation properties to the myeloproliferative sarcoma virus.

Stocking, C, Kollek, R, Bergholz, U, Ostertag, W

The myeloproliferative sarcoma virus not only transforms fibroblasts but also causes extensive expansion of the hematopoietic stem cell compartment on infection of adult mice. Similar to the Moloney...

Changes in genome composition of the Friend virus complex in erythroleukemia cells during the course of differentiation induced by dimethyl sulfoxide

Ostertag, W., Pragnell, I. B.

The Friend spleen focus-forming virus (SFFV) complex released by Friend virus-transformed erythroid cells has been analyzed with respect to changes in the genome composition that may occur during...

Ribonucleic Acid Components of Murine Sarcoma and Leukemia Viruses

Maisel, J., Klement, V., Lai, M. M-C., Ostertag, W., Duesberg, P.

Defective Kirsten murine sarcoma virus was present as leukemia virus pseudotype [Ki-MSV(MLV)] in a 10- to 100-fold excess over its helper, Kirsten murine leukemia virus (Ki-MLV), when the two viruses...

Dimethylsulfoxide-induced differentiation and hemoglobin synthesis in tissue cultures of rat erythroleukemia cells transformed by 7,12-dimethylbenz(a)anthracene.

Kluge, N, Ostertag, W, Sugiyama, T, Arndt-Jovin, D, Steinheider, G, Furusawa, M, ...

Permanent cell lines from transplantable tumors from 7, 12-dimethylbenz(a)anthracene-induced erythroleukemia of the rat were established. These cell lines maintain their erythroid nature. Erythroid...

Induction of endogenous and of spleen focus-forming viruses during dimethylsulfoxide-induced differentiation of mouse erythroleukemia cells transformed by spleen focus-forming virus.

Dube, S K, Pragnell, I B, Kluge, N, Gaedicke, G, Steinheider, G, Ostertag, W

Spleen focus-forming virus-transformed erythroleukemic cell clones, which have been established by infection of N type mice with NB trophic Friend virus, continue to release biologically active...

Induction of Endogenous Virus and of Thymidine Kinase by Bromodeoxyuridine in Cell Cultures Transformed by Friend Virus

Ostertag, W., Roesler, G., Krieg, C. J., Kind, J., Cole, T., Crozier, T., ...

Thymidine kinase positive (TK+) N type cell lines that had been transformed by spleen focus-forming virus were established by transformation with NB tropic Friend virus complex. Thymidine kinase...

Proteoglycan synthesis in human and murine haematopoietic progenitor cell lines: isolation and characterization of a heparan sulphate proteoglycan as a major proteoglycan from the human haematopoietic cell line TF-1.

Stöcker, G, Drzeniek, Z, Just, U, Ostertag, W, Siebertz, B, Greiling, H, ...

Proteoglycans of bone-marrow stromal cells and their extracellular matrix are important components of the microenvironment of haematopoietic tissues. Proteoglycans might also be involved in the...

Proteoglycan synthesis in haematopoietic cells: isolation and characterization of heparan sulphate proteoglycans expressed by the bone-marrow stromal cell line MS-5.

Drzeniek, Z, Siebertz, B, Stöcker, G, Just, U, Ostertag, W, Greiling, H, ...

Proteoglycans of bone-marrow stromal cells and their extracellular matrix are important components of the haematopoietic microenvironment. Recently, several studies have indicated that they are...