Lankenau, D H, Corces, V G, Engels, W R
P element-induced gene conversion has been previously used to modify the white gene of Drosophila melanogaster in a directed fashion. The applicability of this approach of gene targeting in...
Estimating genetic divergence and genetic variability with restriction endonucleases.
Restriction endonucleases cut DNA at specific sites determined by the local nucleotide sequence. By comparing related DNA segments with respect to where such cuts are made, one can estimate the...
Efficient copying of nonhomologous sequences from ectopic sites via P-element-induced gap repair.
Nassif, N, Penney, J, Pal, S, Engels, W R, Gloor, G B
P-element-induced gap repair was used to copy nonhomologous DNA into the Drosophila white locus. We found that nearly 8,000 bp of nonhomologous sequence could be copied from an ectopic template at...
Johnson-Schlitz, D M, Engels, W R
We studied the process by which whd, a P-element insertion allele of the Drosophila melanogaster white locus, is replaced by its homolog in the presence of transposase. These events are interpreted...
Extrachromosomal control of mutability in Drosophila melanogaster.
Hybrid dysgenesis is a syndrome of germ-line aberrations including, e.g., sterility and mutation, found in certain interstrain hybrids of Drosophila melanogaster. Previous studies of sterility have...
Base ratio, DNA content, and quinacrine-brightness of human chromosomes.
Human chromosomes were labeled with base-specific radioactive DNA precursors and examined autoradiographically to measure their DNA content and base ratio (percentage A-T base pairs). The requirement...
Evolution of altruistic behavior by kin selection: an alternative approach.
The evolution of altruistic and selfish behavior by kin selection has been analyzed previously by asking which types of behavior are favored by natural selection. A type of behavior is defined as a...
A Stable Genomic Source of P Element Transposase in Drosophila Melanogaster
Robertson, H. M., Preston, C. R., Phillis, R. W., Johnson-Schlitz, D. M., Benz, W. K., Engels, W. R.
A single P element insert in Drosophila melanogaster, called P[ry(+)Δ2-3](99B), is described that causes mobilization of other elements at unusually high frequencies, yet is itself remarkably...
Modified P Elements That Mimic the P Cytotype in Drosophila Melanogaster
Robertson, H. M., Engels, W. R.
Activity of the P family of transposable elements in Drosophila melanogaster is regulated primarily by a cellular condition known as P cytotype. It has been hypothesized that P cytotype depends on a...
Sved, J. A., Eggleston, W. B., Engels, W. R.
The P element insertion Δ2-3(99B) has previously been shown to activate incomplete P elements elsewhere in the genome. We show that this element, in conjunction with a second incomplete P element,...
Type I Repressors of P Element Mobility
Gloor, G. B., Preston, C. R., Johnson-Schlitz, D. M., Nassif, N. A., Phillis, R. W., Benz, W. K., ...
We describe here a family of P elements that we refer to as type I repressors. These elements are identified by their repressor functions and their lack of any deletion within the first two-thirds of...
P-Element-Induced Male Recombination and Gene Conversion in Drosophila
A P-element insertion flanked by 13 restriction fragment length polymorphism (RFLP) marker sites was used to examine male recombination and gene conversion at an autosomal site. The great majority of...
Flanking Duplications and Deletions Associated with P-Induced Male Recombination in Drosophila
Preston, C. R., Sved, J. A., Engels, W. R.
We studied P element-induced recombination in germline mitotic cells by examining the structure of the recombinant chromosomes. We found that most recombinants retain a mobile P element at the site...
Kusano, K, Berres, M E, Engels, W R
Several eukaryotic homologs of the Escherichia coli RecQ DNA helicase have been found. These include the human BLM gene, whose mutation results in Bloom syndrome, and the human WRN gene, whose...
Lankenau, D H, Corces, V G, Engels, W R
P element-induced gene conversion has been previously used to modify the white gene of Drosophila melanogaster in a directed fashion. The applicability of this approach of gene targeting in...
Estimating genetic divergence and genetic variability with restriction endonucleases.
Restriction endonucleases cut DNA at specific sites determined by the local nucleotide sequence. By comparing related DNA segments with respect to where such cuts are made, one can estimate the...
Efficient copying of nonhomologous sequences from ectopic sites via P-element-induced gap repair.
Nassif, N, Penney, J, Pal, S, Engels, W R, Gloor, G B
P-element-induced gap repair was used to copy nonhomologous DNA into the Drosophila white locus. We found that nearly 8,000 bp of nonhomologous sequence could be copied from an ectopic template at...
Johnson-Schlitz, D M, Engels, W R
We studied the process by which whd, a P-element insertion allele of the Drosophila melanogaster white locus, is replaced by its homolog in the presence of transposase. These events are interpreted...
Extrachromosomal control of mutability in Drosophila melanogaster.
Hybrid dysgenesis is a syndrome of germ-line aberrations including, e.g., sterility and mutation, found in certain interstrain hybrids of Drosophila melanogaster. Previous studies of sterility have...
Base ratio, DNA content, and quinacrine-brightness of human chromosomes.
Human chromosomes were labeled with base-specific radioactive DNA precursors and examined autoradiographically to measure their DNA content and base ratio (percentage A-T base pairs). The requirement...
Evolution of altruistic behavior by kin selection: an alternative approach.
The evolution of altruistic and selfish behavior by kin selection has been analyzed previously by asking which types of behavior are favored by natural selection. A type of behavior is defined as a...
A Stable Genomic Source of P Element Transposase in Drosophila Melanogaster
Robertson, H. M., Preston, C. R., Phillis, R. W., Johnson-Schlitz, D. M., Benz, W. K., Engels, W. R.
A single P element insert in Drosophila melanogaster, called P[ry(+)Δ2-3](99B), is described that causes mobilization of other elements at unusually high frequencies, yet is itself remarkably...
Modified P Elements That Mimic the P Cytotype in Drosophila Melanogaster
Robertson, H. M., Engels, W. R.
Activity of the P family of transposable elements in Drosophila melanogaster is regulated primarily by a cellular condition known as P cytotype. It has been hypothesized that P cytotype depends on a...
Sved, J. A., Eggleston, W. B., Engels, W. R.
The P element insertion Δ2-3(99B) has previously been shown to activate incomplete P elements elsewhere in the genome. We show that this element, in conjunction with a second incomplete P element,...
Type I Repressors of P Element Mobility
Gloor, G. B., Preston, C. R., Johnson-Schlitz, D. M., Nassif, N. A., Phillis, R. W., Benz, W. K., ...
We describe here a family of P elements that we refer to as type I repressors. These elements are identified by their repressor functions and their lack of any deletion within the first two-thirds of...
P-Element-Induced Male Recombination and Gene Conversion in Drosophila
A P-element insertion flanked by 13 restriction fragment length polymorphism (RFLP) marker sites was used to examine male recombination and gene conversion at an autosomal site. The great majority of...
Flanking Duplications and Deletions Associated with P-Induced Male Recombination in Drosophila
Preston, C. R., Sved, J. A., Engels, W. R.
We studied P element-induced recombination in germline mitotic cells by examining the structure of the recombinant chromosomes. We found that most recombinants retain a mobile P element at the site...
Kusano, K, Berres, M E, Engels, W R
Several eukaryotic homologs of the Escherichia coli RecQ DNA helicase have been found. These include the human BLM gene, whose mutation results in Bloom syndrome, and the human WRN gene, whose...