A plasmid transformation system for Rhodococcus sp. strain H13-A was developed by using an Escherichia coli-Rhodococcus shuttle plasmid constructed in this study. Rhodococcus sp. strain H13-A...
Transformation and mobilization of cloning vectors in Acinetobacter spp.
Singer, J T, Van Tuijl, J J, Finnerty, W R
R300B-, RSF1010-, and RK2-derived plasmids were introduced into Acinetobacter sp. strain HO1-N and Acinetobacter calcoaceticus BD413 by transformation and conjugal mobilization. The transformation...
Insertional specificity of transposon Tn5 in Acinetobacter sp.
Suicide plasmid pJB4JI, containing transposon Tn5 and phage Mu, was introduced from Escherichia coli 1830 into Acinetobacter sp. strain HO1-N and Acinetobacter calcoaceticus BD413....
Ensley, B D, Irwin, R M, Carreira, L A, Hoffman, P S, Morgan, T V, Finnerty, W R
The relationship between respiratory chain composition and efficiency of coupling phosphorylation to electron transport was examined in Acinetobacter sp. strain HO1-N. Cells containing only...
Partition of alkane by an extracellular vesicle derived from hexadecane-grown Acinetobacter.
The enhanced solubility of hexadecane in the growth medium of hexadecane-grown Acinetobacter species has been related to the accumulation of an extracellular vesicular component. The partition of...
Torregrossa, R E, Makula, R A, Finnerty, W R
An outer membrane phospholipase A active against phosphatidylglycerol and phosphatidylethanolamine was characterized from Acinetobacter sp. HO1-N.
Singer, M E, Tyler, S M, Finnerty, W R
The growth of Acinetobacter sp. strain HO1-N on hexadecanol results in the formation of intracytoplasmic membranes and intracellular rectangular inclusions containing one of the end products of...
Fatty aldehyde dehydrogenases in Acinetobacter sp. strain HO1-N: role in hexadecanol metabolism.
The role of fatty aldehyde dehydrogenases (FALDHs) in hexadecane and hexadecanol metabolism was studied in Acinetobacter sp. strain HO1-N. Two distinct FALDHs were demonstrated in Acinetobacter sp....
Multiple alcohol dehydrogenases (ADH) were demonstrated in Acinetobacter sp. strain HO1-N. ADH-A and ADH-B were distinguished on the basis of electrophoretic mobility, pyridine nucleotide cofactor...
Phospholipid composition and phospholipase A activity of Neisseria gonorrhoeae.
Senff, L M, Wegener, W S, Brooks, G F, Finnerty, W R, Makula, R A
Exponential-phase cells of Neisseria gonorrhaeae 2686 were examined for phospholipid composition and for membrane-associated phospholipase A activity. When cells were harvested by centrifugation,...
Isolation and characterization of membranes from a hydrocarbon-oxidizing Acinetobacter sp.
Scott, C C, Makula, S R, Finnerty, W R
Membranes were isolated and purified from nutrient broth-yeast extract- and hexadecane-grown cells of Acinetobacter sp. strain HO1-N. Two membrane fractions were isolated from nutrient broth-yeast...
The ultrastructure of Acinetobacter sp. strain HO1-N grown on hydrocarbon and nonhydrocarbon substrates was compared using thin sections and freeze-etching. Hydrocarbon-grown cells were characterized...
Metabolism of the alkane analogue n-dioctyl ether by Acinetobacter species.
Modrzakowski, M C, Makula, R A, Finnerty, W R
Metabolism of n-dioctyl ether by Acinetobacter species HO1-N resulted in formation of 8-n-octoxy-1-octanoic acid and 2-n-octoxy-1-acetic acid. The 16-carbon ether acid was incorporated into the...
Characterization of lysocardiolipin from Acinetobacter sp. HO1-N.
Torregrossa, R E, Makula, R A, Finnerty, W R
Triacyl-lysocardiolipin (triacyl-LCL) and diacyl-LCL were isolated from Acinetobacter sp. HO1-N, and their structures were determined by chemical, physical, and enzymatic procedures. Deacylation of...
Outer membrane phospholipase A from Acinetobacter sp. HO1-N.
Torregrossa, R E, Makula, R A, Finnerty, W R
A phospholipase A1 activity that hydrolyzed cardiolipin to triacyl- and diacyl-lysocardiolipin was localized in outer membrane preparations derived from Acinetobacter sp. HO1-N. The specific activity...
Isolation and characterization of an ornithine-containing lipid from Desulfovibrio gigas.
The isolation and characterization of an ornithine-containing lipid obtained from Desulfovibrio gigas are reported. The general structure for this aminolipid is represented by...
Plasmid-mediated degradation of dibenzothiophene by Pseudomonas species.
Monticello, D J, Bakker, D, Finnerty, W R
The microbial transformation of dibenzothiophene (DBT) is of interest in the potential desulfurization of oil. We isolated three soil Pseudomonas species which oxidized DBT to characteristic...
Plasmid-borne Tn5 insertion mutation resulting in accumulation of gentisate from salicylate.
Monticello, D J, Bakker, D, Schell, M, Finnerty, W R
Plasmid-borne Tn5 insertion mutants of a Pseudomonas species which accumulated 2,5-dihydroxybenzoate (gentisate) following growth on 2-hydroxybenzoate (salicylate) were obtained from a pool of...
Phospholipid Composition of Desulfovibrio Species
Makula, R. A., Finnerty, W. R.
The phospholipids of Desulfovibrio desulfuricans, Norway strain, D. vulgaris, and D. gigas were examined in relationship to their qualitative and quantitative composition. D. desulfuricans and D....
Microbial Assimilation of Hydrocarbons: Phospholipid Metabolism
Makula, R. A., Finnerty, W. R.
An analysis of the turnover of the major phospholipids of Micrococcus cerificans growing or nongrowing cultures. The turnover rates of 14C-PE and 14C-PE were 61.5% of the total phospholipid,...
Microbial Assimilation of Hydrocarbons: Identification of Phospholipids
Makula, R. A., Finnerty, W. R.
The distribution of phospholipids derived from Micrococcus cerificans was determined under a variety of nutritive conditions. Cells were grown with hexadecane, heptadecane, or acetate serving as the...
Microbial Assimilation of Hydrocarbons: Cellular Distribution of Fatty Acids
Makula, R. A., Finnerty, W. R.
The distribution of cellular fatty acids in defined lipid classes was analyzed in Micrococcus cerificans after growth on specified hydrocarbons. Neutral lipid, phospholipid, and cell residue fatty...
Finnerty, W. R., Kallio, R. E.
Finnerty, W. R. (University of Iowa, Iowa City), and R. E. Kallio. Origin of palmitic acid carbon in palmitates formed from hexadecane-1-C14 and tetradecane-1-C14 by Micrococcus cerificans. J....
Comparative analysis of the lipids of Acinetobacter species grown on hexadecane.
Makula, R A, Lockwood, P J, Finnerty, W R
A comparative analysis of the cellular and extracellular lipids of Acinetobacter species HO1-N indicated basic physiological differences in hexadecane-grown cells. The cellular lipids obtained from...
The electron transport system of Acinetobacter sp. HO1-N was studied to determine the specific cytochromes and to measure changes in the composition of the respiratory system due to growth in various...
Microbial Assimilation of Hydrocarbons I. Fatty Acids Derived from Normal Alkanes
Fatty acids derived from Micrococcus cerificans growing at the expense of odd- and even-carbon normal alkanes were studied. Results demonstrated that cultures grown with a variety of nonhydrocarbon...
Microbial Assimilation of Hydrocarbons II. Fatty Acids Derived from 1-Alkenes
The utilization of 1-alkenes by Micrococcus cerificans was investigated with respect to characteristic fatty acid profiles resulting from growth at the expense of these substrates. Saturated fatty...
A plasmid transformation system for Rhodococcus sp. strain H13-A was developed by using an Escherichia coli-Rhodococcus shuttle plasmid constructed in this study. Rhodococcus sp. strain H13-A...
Transformation and mobilization of cloning vectors in Acinetobacter spp.
Singer, J T, Van Tuijl, J J, Finnerty, W R
R300B-, RSF1010-, and RK2-derived plasmids were introduced into Acinetobacter sp. strain HO1-N and Acinetobacter calcoaceticus BD413 by transformation and conjugal mobilization. The transformation...
Insertional specificity of transposon Tn5 in Acinetobacter sp.
Suicide plasmid pJB4JI, containing transposon Tn5 and phage Mu, was introduced from Escherichia coli 1830 into Acinetobacter sp. strain HO1-N and Acinetobacter calcoaceticus BD413....
Ensley, B D, Irwin, R M, Carreira, L A, Hoffman, P S, Morgan, T V, Finnerty, W R
The relationship between respiratory chain composition and efficiency of coupling phosphorylation to electron transport was examined in Acinetobacter sp. strain HO1-N. Cells containing only...
Partition of alkane by an extracellular vesicle derived from hexadecane-grown Acinetobacter.
The enhanced solubility of hexadecane in the growth medium of hexadecane-grown Acinetobacter species has been related to the accumulation of an extracellular vesicular component. The partition of...
Torregrossa, R E, Makula, R A, Finnerty, W R
An outer membrane phospholipase A active against phosphatidylglycerol and phosphatidylethanolamine was characterized from Acinetobacter sp. HO1-N.
Singer, M E, Tyler, S M, Finnerty, W R
The growth of Acinetobacter sp. strain HO1-N on hexadecanol results in the formation of intracytoplasmic membranes and intracellular rectangular inclusions containing one of the end products of...
Fatty aldehyde dehydrogenases in Acinetobacter sp. strain HO1-N: role in hexadecanol metabolism.
The role of fatty aldehyde dehydrogenases (FALDHs) in hexadecane and hexadecanol metabolism was studied in Acinetobacter sp. strain HO1-N. Two distinct FALDHs were demonstrated in Acinetobacter sp....
Multiple alcohol dehydrogenases (ADH) were demonstrated in Acinetobacter sp. strain HO1-N. ADH-A and ADH-B were distinguished on the basis of electrophoretic mobility, pyridine nucleotide cofactor...
Phospholipid composition and phospholipase A activity of Neisseria gonorrhoeae.
Senff, L M, Wegener, W S, Brooks, G F, Finnerty, W R, Makula, R A
Exponential-phase cells of Neisseria gonorrhaeae 2686 were examined for phospholipid composition and for membrane-associated phospholipase A activity. When cells were harvested by centrifugation,...
Isolation and characterization of membranes from a hydrocarbon-oxidizing Acinetobacter sp.
Scott, C C, Makula, S R, Finnerty, W R
Membranes were isolated and purified from nutrient broth-yeast extract- and hexadecane-grown cells of Acinetobacter sp. strain HO1-N. Two membrane fractions were isolated from nutrient broth-yeast...
The ultrastructure of Acinetobacter sp. strain HO1-N grown on hydrocarbon and nonhydrocarbon substrates was compared using thin sections and freeze-etching. Hydrocarbon-grown cells were characterized...
Metabolism of the alkane analogue n-dioctyl ether by Acinetobacter species.
Modrzakowski, M C, Makula, R A, Finnerty, W R
Metabolism of n-dioctyl ether by Acinetobacter species HO1-N resulted in formation of 8-n-octoxy-1-octanoic acid and 2-n-octoxy-1-acetic acid. The 16-carbon ether acid was incorporated into the...
Characterization of lysocardiolipin from Acinetobacter sp. HO1-N.
Torregrossa, R E, Makula, R A, Finnerty, W R
Triacyl-lysocardiolipin (triacyl-LCL) and diacyl-LCL were isolated from Acinetobacter sp. HO1-N, and their structures were determined by chemical, physical, and enzymatic procedures. Deacylation of...
Outer membrane phospholipase A from Acinetobacter sp. HO1-N.
Torregrossa, R E, Makula, R A, Finnerty, W R
A phospholipase A1 activity that hydrolyzed cardiolipin to triacyl- and diacyl-lysocardiolipin was localized in outer membrane preparations derived from Acinetobacter sp. HO1-N. The specific activity...
Isolation and characterization of an ornithine-containing lipid from Desulfovibrio gigas.
The isolation and characterization of an ornithine-containing lipid obtained from Desulfovibrio gigas are reported. The general structure for this aminolipid is represented by...
Plasmid-mediated degradation of dibenzothiophene by Pseudomonas species.
Monticello, D J, Bakker, D, Finnerty, W R
The microbial transformation of dibenzothiophene (DBT) is of interest in the potential desulfurization of oil. We isolated three soil Pseudomonas species which oxidized DBT to characteristic...
Plasmid-borne Tn5 insertion mutation resulting in accumulation of gentisate from salicylate.
Monticello, D J, Bakker, D, Schell, M, Finnerty, W R
Plasmid-borne Tn5 insertion mutants of a Pseudomonas species which accumulated 2,5-dihydroxybenzoate (gentisate) following growth on 2-hydroxybenzoate (salicylate) were obtained from a pool of...
Phospholipid Composition of Desulfovibrio Species
Makula, R. A., Finnerty, W. R.
The phospholipids of Desulfovibrio desulfuricans, Norway strain, D. vulgaris, and D. gigas were examined in relationship to their qualitative and quantitative composition. D. desulfuricans and D....
Microbial Assimilation of Hydrocarbons: Phospholipid Metabolism
Makula, R. A., Finnerty, W. R.
An analysis of the turnover of the major phospholipids of Micrococcus cerificans growing or nongrowing cultures. The turnover rates of 14C-PE and 14C-PE were 61.5% of the total phospholipid,...
Microbial Assimilation of Hydrocarbons: Identification of Phospholipids
Makula, R. A., Finnerty, W. R.
The distribution of phospholipids derived from Micrococcus cerificans was determined under a variety of nutritive conditions. Cells were grown with hexadecane, heptadecane, or acetate serving as the...
Microbial Assimilation of Hydrocarbons: Cellular Distribution of Fatty Acids
Makula, R. A., Finnerty, W. R.
The distribution of cellular fatty acids in defined lipid classes was analyzed in Micrococcus cerificans after growth on specified hydrocarbons. Neutral lipid, phospholipid, and cell residue fatty...
Finnerty, W. R., Kallio, R. E.
Finnerty, W. R. (University of Iowa, Iowa City), and R. E. Kallio. Origin of palmitic acid carbon in palmitates formed from hexadecane-1-C14 and tetradecane-1-C14 by Micrococcus cerificans. J....
Comparative analysis of the lipids of Acinetobacter species grown on hexadecane.
Makula, R A, Lockwood, P J, Finnerty, W R
A comparative analysis of the cellular and extracellular lipids of Acinetobacter species HO1-N indicated basic physiological differences in hexadecane-grown cells. The cellular lipids obtained from...
The electron transport system of Acinetobacter sp. HO1-N was studied to determine the specific cytochromes and to measure changes in the composition of the respiratory system due to growth in various...
Microbial Assimilation of Hydrocarbons I. Fatty Acids Derived from Normal Alkanes
Fatty acids derived from Micrococcus cerificans growing at the expense of odd- and even-carbon normal alkanes were studied. Results demonstrated that cultures grown with a variety of nonhydrocarbon...
Microbial Assimilation of Hydrocarbons II. Fatty Acids Derived from 1-Alkenes
The utilization of 1-alkenes by Micrococcus cerificans was investigated with respect to characteristic fatty acid profiles resulting from growth at the expense of these substrates. Saturated fatty...