Propeller-Induced Velocity Field by Means of Unsteady Lifting Surface Theory. (2005)
An analysis based on lifting surface theory has been developed for evaluation of the vibratory velocity field induced by an operating marine propeller in both uniform and nonuniform inflow fields....
Propeller-Rudder Interaction Due to Loading and Thickness Effects. (2005)
Tsakonas,S., Jacobs,W. R., Ali,M. R.
The previous analysis of the propeller-rudder interaction problem by means of the lifting surface theory has been modified to include the effects of thickness of both surfaces. The effect of...
Propeller-Induced Velocity Field due to Thickness and Loading Effects. (2005)
Blade thickness palys a dual role, contributing to the lifting characteristics of the blade because of its nonplanar form as well as to its non-lifting characteristics due to the generation of a...
UNSTEADY PROPELLER LIFTING-SURFACE THEORY WITH FINITE NUMBER OF CHORDWISE MODES, (2005)
Tsakonas,S., Jacobs,W. R., Rank,P. H. , Jr
A continuing investigation is concerned with improvement of the mathematical model developed for the evaluation of the steady and time-dependent loading distributions on the blades of marine...
An Exact Linear Lifting-Surface Theory for a Marine Propeller in a Nonuniform Flow Field. (2005)
Tsakonas,S., Jacobs,W. R., Ali,M. R.
The mathematical model used in previous Davidson Laboratory adaptations of linearized unsteady lifting surface theory to marine propellers has been revised by removing the so-called 'staircase'...
Tsakonas,S., Chen,C.Y., Jacobs,W.R.
In one instance, an infinitely long elastic beam with circular cross section and in another an in finite elastic plate are immersed in an acoustic fluid parallel to an operating marine propeller. The...
Propeller-Duct Interaction Due to Loading and Thickness Effects. (2002)
Tsakonas,S., Jacobs,W. R., Ali,M. R.
This study is a continuation of an earlier investigation dealing with the interaction of a propeller and its enshrouding nozzle when both are operating in a nonuniform inflow field. The present...
Propeller Blade Pressure Distribution due to Loading and Thickness Effects. (2002)
Tsakonas,S., Jacobs,W. R., Ali,M. R.
A theoretical approach is developed and a computational procedure adaptable to a high speed digital computer is established for the evaluation of the blade pressure distribution of a marine propeller...
Blade Pressure Distribution for a Moderately Loaded Propeller. (2002)
Tsakonas,S., Breslin,John P., Jacobs,W. R.
A new theoretical procedure has been developed modifying the existing analysis for a marine propeller operating in a nonuniform inflow field by considering the radially varying mean wake and mean...
The Linearized Unsteady Lifting Surface Theory Applied to the Pump-Jet Propulsive System. (2002)
Jacobs,W. R., Tsakonas,S., Liao,Ping
A theoretical analysis is developed treating the 'Pump-Jet' propulsive unit comprised of stator, rotor and enshrouding nozzle by using the unsteady lifting surface theory. The analysis takes into...
A THEORY FOR THE PROPELLER-RUDDER INTERACTION. (1998)
Tsakonas,S., Jacobs,W. R., Ali,M. R.
The propeller-rudder interaction problem is studied by means of the unsteady lifting-surface theory. Both surfaces of arbitrary geometry are immersed in a nonuniform flowfield (i.e., hull wake) of an...
Theory and Measurements of the Propeller-Induced Vibratory Field. (1998)
Jacobs,W. R., Mercier,J., Tsakonas,S.
A theory has been developed, based on lifting surface theory, for evaluation of the pressure field generated by an operating propeller in a non-uniform inflow field. In addition, an experimental...
Lifting Surface Theory and Hydroelastic Instability. (1998)
Tsakonas,S., Jacobs,W. R., Ali,M. R.
A brief review is presented of the unsteady lifting surface theory and of the 'generalized lift operator' technique of inverting the 'downwash' integral equation. The integral equation approach is...
Counterrotating Propulsive System. (1998)
Taskonas,S., Jacobs,W. R., Liao,P.
Linearized unsteady lifting surface theory has been applied in the study of counterrotating propeller systems with equal or unequal number of blades operating in uniform or nonuniform inflow fields...
Philipp, W J, Poulet, S, Eiglmeier, K, Pascopella, L, Balasubramanian, V, Heym, B, ...
An integrated map of the genome of the tubercle bacillus, Mycobacterium tuberculosis, was constructed by using a twin-pronged approach. Pulsed-field gel electrophoretic analysis enabled cleavage...
Takiff, H E, Cimino, M, Musso, M C, Weisbrod, T, Martinez, R, Delgado, M B, ...
Due to the resurgence of tuberculosis and the emergence of multidrug-resistant strains, fluoroquinolones (FQ) are being used in selected tuberculosis patients, but FQ-resistant strains of...
Collins, D M, Kawakami, R P, De Lisle, G W, Pascopella, L, Bloom, B R, Jacobs, W R
Tuberculosis continues to be responsible for the deaths of millions of people, yet the virulence factors of the causative pathogens remain unknown. Genetic complementation experiments with strains of...
Lee, M H, Pascopella, L, Jacobs, W R, Hatfull, G F
Mycobacteriophage L5, a temperate phage of mycobacteria, integrates site-specifically into the Mycobacterium smegmatis chromosome. We have identified the int gene and attP site of L5, characterized...
Insertional mutagenesis and illegitimate recombination in mycobacteria.
Kalpana, G V, Bloom, B R, Jacobs, W R
Mycobacteria, particularly Mycobacterium tuberculosis, Mycobacterium leprae, and Mycobacterium avium, are major pathogens of man. Although insertional mutagenesis has been an invaluable genetic tool...
Banaiee, N., Bobadilla-del-Valle, M., Bardarov, S., Riska, P. F., Small, P. M., Ponce-de-Leon, A., ...
The utility of luciferase reporter mycobacteriophages (LRPs) for detection, identification, and antibiotic susceptibility testing of Mycobacterium tuberculosis was prospectively evaluated in a...
Ethambutol resistance in Mycobacterium tuberculosis: critical role of embB mutations.
Sreevatsan, S, Stockbauer, K E, Pan, X, Kreiswirth, B N, Moghazeh, S L, Jacobs, W R, ...
Ethambutol [(S,S')-2,2'-(ethylenediimino)di-1-butanol; EMB], is a first-line drug used to treat tuberculosis. To gain insight into the molecular basis of EMB resistance, we characterized the 10-kb...
McAdam, R A, Weisbrod, T R, Martin, J, Scuderi, J D, Brown, A M, Cirillo, J D, ...
Insertional mutagenesis in Mycobacterium bovis BCG, a member of the slow-growing M. tuberculosis complex, was accomplished with transposons engineered from the Mycobacterium smegmatis insertion...
Leucine auxotrophy restricts growth of Mycobacterium bovis BCG in macrophages.
Bange, F C, Brown, A M, Jacobs, W R
The ability of slow-growing mycobacteria to replicate within host mononuclear phagocytes is thought to be central to the pathogenesis of mycobacterial infection. However, because of the lack of a...
Allelic exchange in Mycobacterium tuberculosis with long linear recombination substrates.
Balasubramanian, V, Pavelka, M S, Bardarov, S S, Martin, J, Weisbrod, T R, McAdam, R A, ...
Genetic studies of Mycobacterium tuberculosis have been greatly hampered by the inability to introduce specific chromosomal mutations. Whereas the ability to perform allelic exchanges has provided a...
Diaminopimelate (DAP) is a unique metabolite used for both the biosynthesis of lysine in bacteria and the construction of the peptidoglycan of many species of bacteria, including mycobacteria. DAP is...
Tuckman, D, Donnelly, R J, Zhao, F X, Jacobs, W R, Connell, N D
Two glycerol utilization mutants of Mycobacterium smegmatis that were unable to utilize most carbon sources except glucose were isolated. Supplementation of these media with small amounts of glucose...
Cloning of the dapB gene, encoding dihydrodipicolinate reductase, from Mycobacterium tuberculosis.
Pavelka, M S, Weisbrod, T R, Jacobs, W R
Diaminopimelate (DAP) is used by bacteria for the synthesis of lysine. In many species of bacteria, including mycobacteria, DAP is also used for peptidoglycan biosynthesis. In this report we describe...
Genetic determination of the meso-diaminopimelate biosynthetic pathway of mycobacteria.
Cirillo, J D, Weisbrod, T R, Banerjee, A, Bloom, B R, Jacobs, W R
The Mycobacterium xenopi GyrA protein splicing element: characterization of a minimal intein.
Telenti, A, Southworth, M, Alcaide, F, Daugelat, S, Jacobs, W R, Perler, F B
The 198-amino-acid in-frame insertion in the gyrA gene of Mycobacterium xenopi is the smallest known naturally occurring active protein splicing element (intein). Comparison with other mycobacterial...
Pascopella, L, Collins, F M, Martin, J M, Lee, M H, Hatfull, G F, Stover, C K, ...
Novel molecular tools and genetic methods were developed to isolate genomic fragments of Mycobacterium tuberculosis that may be associated with virulence. We sought to restore virulence, a...
Cooksey, R C, Crawford, J T, Jacobs, W R, Shinnick, T M
We developed a rapid method to screen the efficacy of antimicrobial agents against Mycobacterium tuberculosis. A restriction fragment carrying a promoterless firefly luciferase gene was cloned into a...
Loci of Mycobacterium avium ser2 gene cluster and their functions.
Mills, J A, McNeil, M R, Belisle, J T, Jacobs, W R, Brennan, P J
The highly antigenic glycopeptidolipids present on the surface of members of the Mycobacterium avium complex serve to distinguish these bacteria from all others and to define the various serovars...
Genetic determination of the meso-diaminopimelate biosynthetic pathway of mycobacteria.
Cirillo, J D, Weisbrod, T R, Banerjee, A, Bloom, B R, Jacobs, W R
The increasing incidence of multiple-drug-resistant mycobacterial infections indicates that the development of new methods for treatment of mycobacterial diseases should be a high priority....
Belisle, J T, Pascopella, L, Inamine, J M, Brennan, P J, Jacobs, W R
Bacteria within the Mycobacterium avium complex are prominent in the environment and are a source of serious disseminated infections in patients with AIDS. Serovars of the M. avium complex are...
A novel transposon trap for mycobacteria: isolation and characterization of IS1096.
Cirillo, J D, Barletta, R G, Bloom, B R, Jacobs, W R
In the course of developing strategies to obtain a mutation in the aspartate semialdehyde dehydrogenase (asd) gene of Mycobacterium smegmatis, an efficient transposon trap was constructed which may...
Molecular analysis of DNA and construction of genomic libraries of Mycobacterium leprae.
Clark-Curtiss, J E, Jacobs, W R, Docherty, M A, Ritchie, L R, Curtiss, R
Molecular analysis of DNA from Mycobacterium leprae, "Mycobacterium lufu," and Mycobacterium vaccae has demonstrated that the G + C (guanine plus cytosine) contents of the DNAs are 56, 61, and 65%,...
Riska, P F, Jacobs, W R, Bloom, B R, McKitrick, J, Chan, J
We have previously described a luciferase reporter mycobacteriophage (LRP) assay that can detect Mycobacterium tuberculosis and characterize mycobacterial drug susceptibility patterns within 24 to 48...
Carrière, C, Riska, P F, Zimhony, O, Kriakov, J, Bardarov, S, Burns, J, ...
TM4 is a lytic mycobacteriophage which infects mycobacteria of clinical importance. A luciferase reporter phage, phAE40, has been constructed from TM4 and was previously shown to be useful for the...
Jacobs, W R, Barrett, J F, Clark-Curtiss, J E, Curtiss, R
Strains of Escherichia coli K-12 were constructed that permitted the amplification of in vitro-packaged recombinant cosmid-transducing particles by in vivo repackaging of recombinant cosmid...
Lysogeny and transformation in mycobacteria: stable expression of foreign genes.
Snapper, S B, Lugosi, L, Jekkel, A, Melton, R E, Kieser, T, Bloom, B R, ...
Requisite to a detailed understanding of the molecular basis of bacterial pathogenesis is a genetic system that allows for the transfer, mutation, and expression of specific genes. Because of the...
Takiff, H E, Salazar, L, Guerrero, C, Philipp, W, Huang, W M, Kreiswirth, B, ...
The emergence of multidrug-resistant strains of Mycobacterium tuberculosis has resulted in increased interest in the fluoroquinolones (FQs) as antituberculosis agents. To investigate the frequency...
Intracellular glutathione in the protection from anoxic injury in renal proximal tubules.
Mandel, L J, Schnellmann, R G, Jacobs, W R
Previous results (Weinberg, J. M., J. A. David, M. Abarzua, and T. Rajan. 1987. J. Clin. Invest. 80:1446-1454) have shown that GSH and glycine (GLY) are cytoprotective during anoxia when added...
Grosskinsky, C M, Jacobs, W R, Clark-Curtiss, J E, Bloom, B R
Comparative DNA hybridization studies of genomic DNA indicated that, while different isolates of armadillo-derived Mycobacterium leprae have a high degree of homology, binding of M. leprae genomic...
Jacobs, W R, Docherty, M A, Curtiss, R, Clark-Curtiss, J E
Genomic libraries of Mycobacterium leprae DNA partially digested with Pst I were constructed in the expression vector pYA626, which contains the promoter region from the Streptococcus mutans gene...
Mycobacterium tuberculosis Lipomannan Induces Apoptosis and Interleukin-12 Production in Macrophages
Dao, D. N., Kremer, L., Guérardel, Y., Molano, A., Jacobs, W. R., Porcelli, S. A., ...
The mycobacterial cell wall component lipoarabinomannan (LAM) has been described as a virulence factor of Mycobacterium tuberculosis, and modification of the terminal arabinan residues of this...
Philipp, W J, Poulet, S, Eiglmeier, K, Pascopella, L, Balasubramanian, V, Heym, B, ...
An integrated map of the genome of the tubercle bacillus, Mycobacterium tuberculosis, was constructed by using a twin-pronged approach. Pulsed-field gel electrophoretic analysis enabled cleavage...
Takiff, H E, Cimino, M, Musso, M C, Weisbrod, T, Martinez, R, Delgado, M B, ...
Due to the resurgence of tuberculosis and the emergence of multidrug-resistant strains, fluoroquinolones (FQ) are being used in selected tuberculosis patients, but FQ-resistant strains of...
Collins, D M, Kawakami, R P, De Lisle, G W, Pascopella, L, Bloom, B R, Jacobs, W R
Tuberculosis continues to be responsible for the deaths of millions of people, yet the virulence factors of the causative pathogens remain unknown. Genetic complementation experiments with strains of...
Lee, M H, Pascopella, L, Jacobs, W R, Hatfull, G F
Mycobacteriophage L5, a temperate phage of mycobacteria, integrates site-specifically into the Mycobacterium smegmatis chromosome. We have identified the int gene and attP site of L5, characterized...
Insertional mutagenesis and illegitimate recombination in mycobacteria.
Kalpana, G V, Bloom, B R, Jacobs, W R
Mycobacteria, particularly Mycobacterium tuberculosis, Mycobacterium leprae, and Mycobacterium avium, are major pathogens of man. Although insertional mutagenesis has been an invaluable genetic tool...
Banaiee, N., Bobadilla-del-Valle, M., Bardarov, S., Riska, P. F., Small, P. M., Ponce-de-Leon, A., ...
The utility of luciferase reporter mycobacteriophages (LRPs) for detection, identification, and antibiotic susceptibility testing of Mycobacterium tuberculosis was prospectively evaluated in a...
Ethambutol resistance in Mycobacterium tuberculosis: critical role of embB mutations.
Sreevatsan, S, Stockbauer, K E, Pan, X, Kreiswirth, B N, Moghazeh, S L, Jacobs, W R, ...
Ethambutol [(S,S')-2,2'-(ethylenediimino)di-1-butanol; EMB], is a first-line drug used to treat tuberculosis. To gain insight into the molecular basis of EMB resistance, we characterized the 10-kb...
McAdam, R A, Weisbrod, T R, Martin, J, Scuderi, J D, Brown, A M, Cirillo, J D, ...
Insertional mutagenesis in Mycobacterium bovis BCG, a member of the slow-growing M. tuberculosis complex, was accomplished with transposons engineered from the Mycobacterium smegmatis insertion...
Leucine auxotrophy restricts growth of Mycobacterium bovis BCG in macrophages.
Bange, F C, Brown, A M, Jacobs, W R
The ability of slow-growing mycobacteria to replicate within host mononuclear phagocytes is thought to be central to the pathogenesis of mycobacterial infection. However, because of the lack of a...
Allelic exchange in Mycobacterium tuberculosis with long linear recombination substrates.
Balasubramanian, V, Pavelka, M S, Bardarov, S S, Martin, J, Weisbrod, T R, McAdam, R A, ...
Genetic studies of Mycobacterium tuberculosis have been greatly hampered by the inability to introduce specific chromosomal mutations. Whereas the ability to perform allelic exchanges has provided a...
Diaminopimelate (DAP) is a unique metabolite used for both the biosynthesis of lysine in bacteria and the construction of the peptidoglycan of many species of bacteria, including mycobacteria. DAP is...
Tuckman, D, Donnelly, R J, Zhao, F X, Jacobs, W R, Connell, N D
Two glycerol utilization mutants of Mycobacterium smegmatis that were unable to utilize most carbon sources except glucose were isolated. Supplementation of these media with small amounts of glucose...
Cloning of the dapB gene, encoding dihydrodipicolinate reductase, from Mycobacterium tuberculosis.
Pavelka, M S, Weisbrod, T R, Jacobs, W R
Diaminopimelate (DAP) is used by bacteria for the synthesis of lysine. In many species of bacteria, including mycobacteria, DAP is also used for peptidoglycan biosynthesis. In this report we describe...
Genetic determination of the meso-diaminopimelate biosynthetic pathway of mycobacteria.
Cirillo, J D, Weisbrod, T R, Banerjee, A, Bloom, B R, Jacobs, W R
The Mycobacterium xenopi GyrA protein splicing element: characterization of a minimal intein.
Telenti, A, Southworth, M, Alcaide, F, Daugelat, S, Jacobs, W R, Perler, F B
The 198-amino-acid in-frame insertion in the gyrA gene of Mycobacterium xenopi is the smallest known naturally occurring active protein splicing element (intein). Comparison with other mycobacterial...
Pascopella, L, Collins, F M, Martin, J M, Lee, M H, Hatfull, G F, Stover, C K, ...
Novel molecular tools and genetic methods were developed to isolate genomic fragments of Mycobacterium tuberculosis that may be associated with virulence. We sought to restore virulence, a...
Cooksey, R C, Crawford, J T, Jacobs, W R, Shinnick, T M
We developed a rapid method to screen the efficacy of antimicrobial agents against Mycobacterium tuberculosis. A restriction fragment carrying a promoterless firefly luciferase gene was cloned into a...
Loci of Mycobacterium avium ser2 gene cluster and their functions.
Mills, J A, McNeil, M R, Belisle, J T, Jacobs, W R, Brennan, P J
The highly antigenic glycopeptidolipids present on the surface of members of the Mycobacterium avium complex serve to distinguish these bacteria from all others and to define the various serovars...
Genetic determination of the meso-diaminopimelate biosynthetic pathway of mycobacteria.
Cirillo, J D, Weisbrod, T R, Banerjee, A, Bloom, B R, Jacobs, W R
The increasing incidence of multiple-drug-resistant mycobacterial infections indicates that the development of new methods for treatment of mycobacterial diseases should be a high priority....
Belisle, J T, Pascopella, L, Inamine, J M, Brennan, P J, Jacobs, W R
Bacteria within the Mycobacterium avium complex are prominent in the environment and are a source of serious disseminated infections in patients with AIDS. Serovars of the M. avium complex are...
A novel transposon trap for mycobacteria: isolation and characterization of IS1096.
Cirillo, J D, Barletta, R G, Bloom, B R, Jacobs, W R
In the course of developing strategies to obtain a mutation in the aspartate semialdehyde dehydrogenase (asd) gene of Mycobacterium smegmatis, an efficient transposon trap was constructed which may...
Molecular analysis of DNA and construction of genomic libraries of Mycobacterium leprae.
Clark-Curtiss, J E, Jacobs, W R, Docherty, M A, Ritchie, L R, Curtiss, R
Molecular analysis of DNA from Mycobacterium leprae, "Mycobacterium lufu," and Mycobacterium vaccae has demonstrated that the G + C (guanine plus cytosine) contents of the DNAs are 56, 61, and 65%,...