W. R. Laws

Spectral enhancement of proteins: biological incorporation and fluorescence characterization of 5-hydroxytryptophan in bacteriophage lambda cI repressor.

Ross, J B, Senear, D F, Waxman, E, Kombo, B B, Rusinova, E, Huang, Y T, ...

We have used a tryptophan-requiring Escherichia coli auxotroph to replace the three tryptophan residues of lambda cI repressor with 5-hydroxy-L-tryptophan (5-OHTrp). By using a nonleaky promoter, we...

Fluorescence properties of a new guanosine analog incorporated into small oligonucleotides.

Driscoll, S L, Hawkins, M E, Balis, F M, Pfleiderer, W, Laws, W R

The fluorescence properties of 3-methyl-isoxanthopterin (3-MI) incorporated into different oligonucleotides have been determined. This highly fluorescent guanosine analog has its absorption and...

Dynamics of biomolecules: assignment of local motions by fluorescence anisotropy decay.

Bialik, C N, Wolf, B, Rachofsky, E L, Ross, J B, Laws, W R

Many biological systems have multiple fluorophores that experience multiple depolarizing motions, requiring multiple lifetimes and correlation times to define the fluorescence intensity and...

Spectral enhancement of proteins: biological incorporation and fluorescence characterization of 5-hydroxytryptophan in bacteriophage lambda cI repressor.

Ross, J B, Senear, D F, Waxman, E, Kombo, B B, Rusinova, E, Huang, Y T, ...

We have used a tryptophan-requiring Escherichia coli auxotroph to replace the three tryptophan residues of lambda cI repressor with 5-hydroxy-L-tryptophan (5-OHTrp). By using a nonleaky promoter, we...

Fluorescence properties of a new guanosine analog incorporated into small oligonucleotides.

Driscoll, S L, Hawkins, M E, Balis, F M, Pfleiderer, W, Laws, W R

The fluorescence properties of 3-methyl-isoxanthopterin (3-MI) incorporated into different oligonucleotides have been determined. This highly fluorescent guanosine analog has its absorption and...

Dynamics of biomolecules: assignment of local motions by fluorescence anisotropy decay.

Bialik, C N, Wolf, B, Rachofsky, E L, Ross, J B, Laws, W R

Many biological systems have multiple fluorophores that experience multiple depolarizing motions, requiring multiple lifetimes and correlation times to define the fluorescence intensity and...

Oxidation of apolipoprotein(a) inhibits kringle-associated lysine binding: the loss of intrinsic protein fluorescence suggests a role for tryptophan residues in the lysine binding site.

Hermann, A., Laws, W. R., Harpel, P. C.

Lipoprotein(a) [Lp(a)] is a low-density lipoprotein complex consisting of apolipoprotein(a) [apo(a)] disulfide-linked to apolipoprotein B-100. Lp(a) has been implicated in atherogenesis and...