Hassa, P O, Haenni, S S, Buerki, C, Meier, N I, Lane, W S, Owen, H, ...
Poly(ADP-ribose) polymerase-1 (PARP-1) and nuclear factor kappaB (NF-kappaB) have both been demonstrated to play a pathophysiological role in a number of inflammatory disorders. We recently presented...
Covalent HLA-B27/peptide complex induced by specific recognition of an aziridine mimic of arginine.
Weiss, G A, Valentekovich, R J, Collins, E J, Garboczi, D N, Lane, W S, Schreiber, S L, ...
The class I major histocompatibility complex (MHC) glycoprotein HLA-B27 binds short peptides containing arginine at peptide position 2 (P2). The HLA-B27/peptide complex is recognized by T cells both...
Crews, C M, Lane, W S, Schreiber, S L
The marine natural product didemnin B, currently in clinical trials as an antitumor agent, has several potent biological activities apparently mediated by distinct mechanisms. Our initial...
Li, Y, Bjorklund, S, Jiang, Y W, Kim, Y J, Lane, W S, Stillman, D J, ...
Sin4 and Rgr1 proteins, previously shown by genetic studies to play both positive and negative roles in the transcriptional regulation of many genes, are identified here as components of mediator and...
Positive regulation of general transcription factor SIII by a tailed ubiquitin homolog.
Garrett, K P, Aso, T, Bradsher, J N, Foundling, S I, Lane, W S, Conaway, R C, ...
General transcription factor SIII, a heterotrimer composed of 110-kDa (p110), 18-kDa (p18), and 15-kDa (p15) subunits, increases the catalytic rate of transcribing RNA polymerase II by suppressing...
Davenport, M P, Quinn, C L, Chicz, R M, Green, B N, Willis, A C, Lane, W S, ...
HLA-DR13 has been associated with resistance to two major infectious diseases of humans. To investigate the peptide binding specificity of two HLA-DR13 molecules and the effects of the Gly/Val...
A subset of HLA-B27 molecules contains peptides much longer than nonamers.
Urban, R G, Chicz, R M, Lane, W S, Strominger, J L, Rehm, A, Kenter, M J, ...
An unusual monoclonal antibody (MARB4) directed against HLA-B27 that reacts with only approximately 5-20% of the cell surface HLA-B27 was used for large-scale purification of these molecules....
Molecular cloning of an essential subunit of RNA polymerase II elongation factor SIII.
Garrett, K P, Tan, S, Bradsher, J N, Lane, W S, Conaway, J W, Conaway, R C
A transcription factor designated SIII was recently purified from mammalian cells and shown to regulate the activity of the RNA polymerase II elongation complex. SIII is a heterotrimer composed of...
Cabrero, J G, Freeman, G J, Lane, W S, Reiser, H
We have recently described a murine lymphocyte protein, provisionally termed sgp-60, which is expressed on virtually all lymphocytes of both T- and B-cell origin. A hamster monoclonal antibody to...
Transmission-blocking antibodies recognize microfilarial chitinase in brugian lymphatic filariasis.
Fuhrman, J A, Lane, W S, Smith, R F, Piessens, W F, Perler, F B
Brugia malayi is a parasitic nematode that causes lymphatic filariasis in humans. The monoclonal antibody MF1, which mediates clearance of peripheral microfilaremia in a gerbil infection model,...
Molecular cloning of a membrane-associated human FK506- and rapamycin-binding protein, FKBP-13.
Jin, Y J, Albers, M W, Lane, W S, Bierer, B E, Schreiber, S L, Burakoff, S J
The 12-kDa FK506-binding protein (FKBP-12) is a cytosolic receptor for the immunosuppressants FK506 and rapamycin. Here we report the molecular cloning and subcellular localization of a 13-kDa FKBP...
Reynolds, D S, Stevens, R L, Lane, W S, Carr, M H, Austen, K F, Serafin, W E
Mouse serosal mast cells (SMCs) and Kirsten sarcoma virus-immortalized mast cells store large amounts of mast cell carboxypeptidase A and serine proteases in their secretory granules. Secretory...
Complete amino acid sequence of bovine glia maturation factor beta.
The protein glia maturation factor beta, isolated from bovine brain, has been sequenced by automated Edman degradation and tandem mass spectrometry of overlapped peptide fragments generated by...
Cloney, L P, Bekkaoui, D R, Feist, G L, Lane, W S, Hemmingsen, S M
Plastid chaperonin-60 protein was purified to apparent homogeneity from Brassica napus using a novel protocol. The purified protein, which migrated as a single species by nondenaturing polyacrylamide...
pCyP B: a chloroplast-localized, heat shock-responsive cyclophilin from fava bean.
Luan, S, Lane, W S, Schreiber, S L
When the immunosuppressants cyclosporin A (CsA) and FK506 bind to their intracellular receptors (immunophillins), they form complexes that bind to calcineurin and block calcineurin-dependent...
Isolation and characterization of the constitutive acyl carrier protein from Rhizobium meliloti.
Platt, M W, Miller, K J, Lane, W S, Kennedy, E P
Rhizobium species produce an inducible acyl carrier protein (ACP), encoded by the nodF gene, that somehow functions in an exchange of cell signals between bacteria and specific plant hosts, leading...
Luo, C, Burgeon, E, Carew, J A, McCaffrey, P G, Badalian, T M, Lane, W S, ...
Transcription factors of the NFAT family play a key role in the transcription of cytokine genes and other genes during the immune response. We have identified two new isoforms of the transcription...
Suñé, C, Hayashi, T, Liu, Y, Lane, W S, Young, R A, Garcia-Blanco, M A
Maximal human immunodeficiency virus type 1 (HIV-1) gene expression requires specific cellular factors in addition to the virus-encoded trans-activator protein Tat and the RNA element TAR. We...
Pallas, D C, Weller, W, Jaspers, S, Miller, T B, Lane, W S, Roberts, T M
The small and middle T (tumor) antigens of polyomavirus have been shown previously to associate with the 36-kDa catalytic subunit and the 63-kDa regulatory subunit of protein phosphatase type 2A,...
Sander, M, Lowenhaupt, K, Lane, W S, Rich, A
We previously reported the purification of a protein from Drosophila embryo extracts that carries out the strand transfer step in homologous recombination (Lowenhaupt, K., Sander, M., Hauser, C. and...
The cellular factor TRP-185 regulates RNA polymerase II binding to HIV-1 TAR RNA.
Wu-Baer, F, Lane, W S, Gaynor, R B
Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element located downstream of the transcription initiation site known as TAR. To characterize cellular...
Glasgow, E, Druger, R K, Fuchs, C, Lane, W S, Schechter, N
The goldfish visual pathway displays a remarkable capacity for continued development and plasticity. The intermediate filament proteins of this pathway do not match the intermediate filament protein...
Lauber, J, Fabrizio, P, Teigelkamp, S, Lane, W S, Hartmann, E, Luhrmann, R
The primary structure of the 200 kDa protein of purified HeLa U5 snRNPs (U5-200kD) was characterized by cloning and sequencing of its cDNA. In order to confirm that U5-200kD is distinct from U5-220kD...
Evidence for a non-alpha-helical DNA-binding motif in the Rel homology region.
Liu, J, Sodeoka, M, Lane, W S, Verdine, G L
The Rel family of transcription factors serve as terminal messengers in a variety of developmental and receptor-mediated signaling pathways. These proteins are related by a domain of approximately...
Glial hyaluronate-binding protein: a product of metalloproteinase digestion of versican?
Perides, G, Asher, R A, Lark, M W, Lane, W S, Robinson, R A, Bignami, A
Glial hyaluronate-binding protein (GHAP) is a 60 kDa glycoprotein with an amino acid sequence identical to that of the hyaluronate-binding region of versican, a large fibroblast aggregating...
Purification of a Tat-associated kinase reveals a TFIIH complex that modulates HIV-1 transcription.
García-Martínez, L F, Mavankal, G, Neveu, J M, Lane, W S, Ivanov, D, Gaynor, R B
The Tat protein is a transcriptional activator which is required for efficient human immunodeficiency virus 1 (HIV-1) gene expression Tat stimulates HIV-1 transcriptional elongation by increasing the...
Fabrizio, P, Laggerbauer, B, Lauber, J, Lane, W S, Lührmann, R
The driving forces behind the many RNA conformational changes occurring in the spliceosome are not well understood. Here we characterize an evolutionarily conserved human U5 small nuclear...
Lauber, J, Plessel, G, Prehn, S, Will, C L, Fabrizio, P, Gröning, K, ...
Immunoaffinity-purified human 25S [U4/U6.U5] tri-snRNPs harbor a set of polypeptides, termed the tri-snRNP proteins, that are not present in Mono Q-purified 20S U5 snRNPs or 10S U4/U6 snRNPs and that...
Teigelkamp, S, Achsel, T, Mundt, C, Göthel, S F, Cronshagen, U, Lane, W S, ...
Cyclophilins (Cyps) catalyze the cis/trans isomerization of peptidyl-prolyl bonds, a rate-limiting step in protein folding. In some cases, cyclophilins have also been shown to form stable complexes...
Covalent HLA-B27/peptide complex induced by specific recognition of an aziridine mimic of arginine.
Weiss, G A, Valentekovich, R J, Collins, E J, Garboczi, D N, Lane, W S, Schreiber, S L, ...
The class I major histocompatibility complex (MHC) glycoprotein HLA-B27 binds short peptides containing arginine at peptide position 2 (P2). The HLA-B27/peptide complex is recognized by T cells both...
Crews, C M, Lane, W S, Schreiber, S L
The marine natural product didemnin B, currently in clinical trials as an antitumor agent, has several potent biological activities apparently mediated by distinct mechanisms. Our initial...
Li, Y, Bjorklund, S, Jiang, Y W, Kim, Y J, Lane, W S, Stillman, D J, ...
Sin4 and Rgr1 proteins, previously shown by genetic studies to play both positive and negative roles in the transcriptional regulation of many genes, are identified here as components of mediator and...
Positive regulation of general transcription factor SIII by a tailed ubiquitin homolog.
Garrett, K P, Aso, T, Bradsher, J N, Foundling, S I, Lane, W S, Conaway, R C, ...
General transcription factor SIII, a heterotrimer composed of 110-kDa (p110), 18-kDa (p18), and 15-kDa (p15) subunits, increases the catalytic rate of transcribing RNA polymerase II by suppressing...
Davenport, M P, Quinn, C L, Chicz, R M, Green, B N, Willis, A C, Lane, W S, ...
HLA-DR13 has been associated with resistance to two major infectious diseases of humans. To investigate the peptide binding specificity of two HLA-DR13 molecules and the effects of the Gly/Val...
A subset of HLA-B27 molecules contains peptides much longer than nonamers.
Urban, R G, Chicz, R M, Lane, W S, Strominger, J L, Rehm, A, Kenter, M J, ...
An unusual monoclonal antibody (MARB4) directed against HLA-B27 that reacts with only approximately 5-20% of the cell surface HLA-B27 was used for large-scale purification of these molecules....
Molecular cloning of an essential subunit of RNA polymerase II elongation factor SIII.
Garrett, K P, Tan, S, Bradsher, J N, Lane, W S, Conaway, J W, Conaway, R C
A transcription factor designated SIII was recently purified from mammalian cells and shown to regulate the activity of the RNA polymerase II elongation complex. SIII is a heterotrimer composed of...
Cabrero, J G, Freeman, G J, Lane, W S, Reiser, H
We have recently described a murine lymphocyte protein, provisionally termed sgp-60, which is expressed on virtually all lymphocytes of both T- and B-cell origin. A hamster monoclonal antibody to...
Transmission-blocking antibodies recognize microfilarial chitinase in brugian lymphatic filariasis.
Fuhrman, J A, Lane, W S, Smith, R F, Piessens, W F, Perler, F B
Brugia malayi is a parasitic nematode that causes lymphatic filariasis in humans. The monoclonal antibody MF1, which mediates clearance of peripheral microfilaremia in a gerbil infection model,...
Molecular cloning of a membrane-associated human FK506- and rapamycin-binding protein, FKBP-13.
Jin, Y J, Albers, M W, Lane, W S, Bierer, B E, Schreiber, S L, Burakoff, S J
The 12-kDa FK506-binding protein (FKBP-12) is a cytosolic receptor for the immunosuppressants FK506 and rapamycin. Here we report the molecular cloning and subcellular localization of a 13-kDa FKBP...
Reynolds, D S, Stevens, R L, Lane, W S, Carr, M H, Austen, K F, Serafin, W E
Mouse serosal mast cells (SMCs) and Kirsten sarcoma virus-immortalized mast cells store large amounts of mast cell carboxypeptidase A and serine proteases in their secretory granules. Secretory...
Complete amino acid sequence of bovine glia maturation factor beta.
The protein glia maturation factor beta, isolated from bovine brain, has been sequenced by automated Edman degradation and tandem mass spectrometry of overlapped peptide fragments generated by...
Cloney, L P, Bekkaoui, D R, Feist, G L, Lane, W S, Hemmingsen, S M
Plastid chaperonin-60 protein was purified to apparent homogeneity from Brassica napus using a novel protocol. The purified protein, which migrated as a single species by nondenaturing polyacrylamide...
pCyP B: a chloroplast-localized, heat shock-responsive cyclophilin from fava bean.
Luan, S, Lane, W S, Schreiber, S L
When the immunosuppressants cyclosporin A (CsA) and FK506 bind to their intracellular receptors (immunophillins), they form complexes that bind to calcineurin and block calcineurin-dependent...
Isolation and characterization of the constitutive acyl carrier protein from Rhizobium meliloti.
Platt, M W, Miller, K J, Lane, W S, Kennedy, E P
Rhizobium species produce an inducible acyl carrier protein (ACP), encoded by the nodF gene, that somehow functions in an exchange of cell signals between bacteria and specific plant hosts, leading...
Luo, C, Burgeon, E, Carew, J A, McCaffrey, P G, Badalian, T M, Lane, W S, ...
Transcription factors of the NFAT family play a key role in the transcription of cytokine genes and other genes during the immune response. We have identified two new isoforms of the transcription...
Suñé, C, Hayashi, T, Liu, Y, Lane, W S, Young, R A, Garcia-Blanco, M A
Maximal human immunodeficiency virus type 1 (HIV-1) gene expression requires specific cellular factors in addition to the virus-encoded trans-activator protein Tat and the RNA element TAR. We...
Pallas, D C, Weller, W, Jaspers, S, Miller, T B, Lane, W S, Roberts, T M
The small and middle T (tumor) antigens of polyomavirus have been shown previously to associate with the 36-kDa catalytic subunit and the 63-kDa regulatory subunit of protein phosphatase type 2A,...
Sander, M, Lowenhaupt, K, Lane, W S, Rich, A
We previously reported the purification of a protein from Drosophila embryo extracts that carries out the strand transfer step in homologous recombination (Lowenhaupt, K., Sander, M., Hauser, C. and...
The cellular factor TRP-185 regulates RNA polymerase II binding to HIV-1 TAR RNA.
Wu-Baer, F, Lane, W S, Gaynor, R B
Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element located downstream of the transcription initiation site known as TAR. To characterize cellular...
Glasgow, E, Druger, R K, Fuchs, C, Lane, W S, Schechter, N
The goldfish visual pathway displays a remarkable capacity for continued development and plasticity. The intermediate filament proteins of this pathway do not match the intermediate filament protein...
Lauber, J, Fabrizio, P, Teigelkamp, S, Lane, W S, Hartmann, E, Luhrmann, R
The primary structure of the 200 kDa protein of purified HeLa U5 snRNPs (U5-200kD) was characterized by cloning and sequencing of its cDNA. In order to confirm that U5-200kD is distinct from U5-220kD...
Evidence for a non-alpha-helical DNA-binding motif in the Rel homology region.
Liu, J, Sodeoka, M, Lane, W S, Verdine, G L
The Rel family of transcription factors serve as terminal messengers in a variety of developmental and receptor-mediated signaling pathways. These proteins are related by a domain of approximately...
Glial hyaluronate-binding protein: a product of metalloproteinase digestion of versican?
Perides, G, Asher, R A, Lark, M W, Lane, W S, Robinson, R A, Bignami, A
Glial hyaluronate-binding protein (GHAP) is a 60 kDa glycoprotein with an amino acid sequence identical to that of the hyaluronate-binding region of versican, a large fibroblast aggregating...
Purification of a Tat-associated kinase reveals a TFIIH complex that modulates HIV-1 transcription.
García-Martínez, L F, Mavankal, G, Neveu, J M, Lane, W S, Ivanov, D, Gaynor, R B
The Tat protein is a transcriptional activator which is required for efficient human immunodeficiency virus 1 (HIV-1) gene expression Tat stimulates HIV-1 transcriptional elongation by increasing the...
Fabrizio, P, Laggerbauer, B, Lauber, J, Lane, W S, Lührmann, R
The driving forces behind the many RNA conformational changes occurring in the spliceosome are not well understood. Here we characterize an evolutionarily conserved human U5 small nuclear...
Lauber, J, Plessel, G, Prehn, S, Will, C L, Fabrizio, P, Gröning, K, ...
Immunoaffinity-purified human 25S [U4/U6.U5] tri-snRNPs harbor a set of polypeptides, termed the tri-snRNP proteins, that are not present in Mono Q-purified 20S U5 snRNPs or 10S U4/U6 snRNPs and that...
Teigelkamp, S, Achsel, T, Mundt, C, Göthel, S F, Cronshagen, U, Lane, W S, ...
Cyclophilins (Cyps) catalyze the cis/trans isomerization of peptidyl-prolyl bonds, a rate-limiting step in protein folding. In some cases, cyclophilins have also been shown to form stable complexes...
ABRF ESRG 2005 Study: Identification of Seven Modified Amino Acids by Edman Sequencing
Brune, D., Denslow, N.D., Kobayashi, R., Lane, W.S., Leone, J.W., Madden, B.J., ...
Identification of modified amino acids can be a challenging part for Edman degradation sequence analysis, largely because they are not included among the commonly used phenylthiohydantion amino acid...
P58-S Optimization of Nanobore Capillary Trapping Column Geometries for Analysis of Peptide Mixtures
Neveu, J. M., Toher, C. J., Lane, W. S., Valaskovic, G. A.
Nanobore reversed-phase HPLC has become a mainstay for protein identification by modern tandem mass spectrometry. Enhanced robustness and versatility is obtained with trapping columns for on-line...