Hoffman, P, Rajakumar, P, Hoffman, B, Heuertz, R, Wold, W S, Carlin, C R
We have reported previously that human group C adenoviruses down-regulate the epidermal growth factor (EGF) receptor (EGF-R) (C. R. Carlin, A. E. Tollefson, H. A. Brady, B. L. Hoffman, and W. S. M....
Stewart, A R, Tollefson, A E, Krajcsi, P, Yei, S P, Wold, W S
The adenovirus type 2 and 5 E3 10,400- and 14,500-molecular-weight (10.4K and 14.5K) proteins are both required to protect some cell lines from lysis by tumor necrosis factor and to down-regulate the...
Hawkins, L K, Wilson-Rawls, J, Wold, W S
There is an open reading frame in the E3 transcription unit of adenovirus type 3 (Ad3) and Ad7 that could encode a protein of 16 kDa (16K protein). Ad3 and Ad7 are members of subgroup B of human...
Shisler, J, Duerksen-Hughes, P, Hermiston, T M, Wold, W S, Gooding, L R
The introduction of the adenovirus early region 1A (E1A) gene products into normal cells sensitizes these cells to the cytotoxic effects of tumor necrosis factor (TNF). Previous studies have shown...
Tollefson, A E, Scaria, A, Hermiston, T W, Ryerse, J S, Wold, L J, Wold, W S
Adenovirus (Ad) infection is concluded by assembly of virions in the cell nucleus followed by lysis of cells by an unknown mechanism. We have described an Ad nuclear membrane glycoprotein of 11,600...
Sparer, T E, Tripp, R A, Dillehay, D L, Hermiston, T W, Wold, W S, Gooding, L R
Products of human adenovirus (Ad) early region 3 (E3) inhibit both specific (cytotoxic T lymphocytes [CTLs]) and innate (tumor necrosis factor alpha [TNF-alpha]) immune responses in vitro. The E3...
Krajcsi, P, Dimitrov, T, Hermiston, T W, Tollefson, A E, Ranheim, T S, Vande Pol, S B, ...
Tumor necrosis factor (TNF) is an inflammatory cytokine that inhibits the replication of many viruses in cultured cells. We have reported that adenovirus (Ad) infection of TNF-resistant mouse cells...
Dimitrov, T, Krajcsi, P, Hermiston, T W, Tollefson, A E, Hannink, M, Wold, W S
We have reported that three adenovirus (Ad) proteins, named E3-10.4K/14.5K, E3-14.7K, and E1B-19K, independently inhibit tumor necrosis factor (TNF)-induced apoptosis in Ad-infected cells....
Hermiston, T W, Tripp, R A, Sparer, T, Gooding, L R, Wold, W S
Adenovirus E3-gp19K is a transmembrane glycoprotein, localized in the endoplasmic reticulum (ER), which forms a complex with major histocompatibility complex (MHC) class I antigens and retains them...
Hoffman, P, Rajakumar, P, Hoffman, B, Heuertz, R, Wold, W S, Carlin, C R
We have reported previously that human group C adenoviruses down-regulate the epidermal growth factor (EGF) receptor (EGF-R) (C. R. Carlin, A. E. Tollefson, H. A. Brady, B. L. Hoffman, and W. S. M....
Duerksen-Hughes, P J, Hermiston, T W, Wold, W S, Gooding, L R
Previous work by our laboratory and others has shown that mouse cells normally resistant to tumor necrosis factor can be made sensitive to the cytokine by the expression of adenovirus E1A. The E1A...
Ranheim, T S, Shisler, J, Horton, T M, Wold, L J, Gooding, L R, Wold, W S
The 14,700-Da protein (14.7K protein) encoded by the E3 region of adenovirus has previously been shown to protect mouse cells from cytolysis by tumor necrosis factor (TNF). Delineating the sequences...
Horton, T M, Ranheim, T S, Aquino, L, Kusher, D I, Saha, S K, Ware, C F, ...
A 14,700-kDa protein (14.7K) encoded by the E3 region of adenovirus has been shown to protect adenovirus-infected mouse C3HA cells from lysis by tumor necrosis factor (TNF) (L. R. Gooding, L. W....
Krajcsi, P, Tollefson, A E, Anderson, C W, Wold, W S
We previously reported that the adenovirus type 5 E3 14.5-kilodalton protein (14.5K) forms a complex with E3 10.4K and that both proteins are required to down-regulate the epidermal growth factor...
Gooding, L R, Aquino, L, Duerksen-Hughes, P J, Day, D, Horton, T M, Yei, S P, ...
Tumor necrosis factor (TNF) is a multifunctional immunoregulatory protein that is secreted by activated macrophages and is believed to have antiviral activities. We reported earlier that when mouse...
Tollefson, A E, Stewart, A R, Yei, S P, Saha, S K, Wold, W S
In adenovirus-infected cells, the epidermal growth factor receptor (EGF-R) is internalized from the cell surface via endosomes and is degraded, and the E3 10,400-dalton protein (10.4K protein) is...
Tollefson, A E, Scaria, A, Saha, S K, Wold, W S
We have reported that an 11,600-MW (11.6K) protein is coded by region E3 of adenovirus. We have now prepared two new antipeptide antisera that have allowed us to characterize this protein further....
Brady, H A, Scaria, A, Wold, W S
The E3 complex transcription unit of adenovirus encodes four major mRNAs (a, c, f, and h) and two minor (d and e) mRNAs with overlapping exons, alternative splice sites, and two polyadenylation...
Gooding, L R, Ranheim, T S, Tollefson, A E, Aquino, L, Duerksen-Hughes, P, Horton, T M, ...
We have reported that the E3 14,700-dalton protein (E3 14.7K protein) protects adenovirus-infected mouse C3HA fibroblasts against lysis by tumor necrosis factor (TNF) (L. R. Gooding, L. W. Elmore, A....
A 10,400-molecular-weight membrane protein is coded by region E3 of adenovirus.
Tollefson, A E, Krajcsi, P, Yei, S P, Carlin, C R, Wold, W S
Previous studies with adenovirus mutants have indicated that a 10,400-molecular-weight (10.4K) protein predicted to be coded by an open reading frame in region E3 of adenovirus functions to down...
Yei, S P, Chowdhury, S I, Bhat, B M, Conley, A J, Wold, W S, Batterson, W
We describe the characterization of the herpes simplex virus type 2 (HSV-2) gene encoding infected cell protein 32 (ICP32) and virion protein 19c (VP19c). We also demonstrate that the HSV-1...
Horton, T M, Tollefson, A E, Wold, W S, Gooding, L R
A 14.7-kilodalton protein (14.7K protein) encoded by the E3 region of group C adenoviruses has been shown to protect virus-infected fibroblasts from lysis by tumor necrosis factor (TNF) (L.R....
Early region E3 of adenovirus type 5 should encode at least nine proteins as judged by the DNA sequence and the spliced structures of the known mRNAs. Only two E3 proteins have been proved to exist,...
Region E3 of adenovirus encodes about 10 overlapping mRNAs with different spliced structures. The mRNAs are 5' coterminal and form two major 3'-coterminal families termed E3A and E3B. As a group, the...
Region E3 of adenovirus encodes about nine overlapping mRNAs (a to i) with different spliced structures and with two major RNA 3' end sites termed E3A and E3B. Synthesis of E3 mRNAs was examined by...
Wold, W S, Cladaras, C, Magie, S C, Yacoub, N
The DNA sequence of the early E3 transcription unit of adenovirus 2 (Ad2) (J. Hérissé et al., Nucleic Acids Res. 8:2173-2192, 1980), indicates that an open reading frame exists between nucleotides...
Takemori, N, Cladaras, C, Bhat, B, Conley, A J, Wold, W S
A total of 59 cytocidal (cyt) mutants were isolated from adenovirus 2 (Ad2) and Ad5. In contrast to the small plaques and adenovirus type of cytopathic effects produced by wild-type cyt+ viruses, the...
The E3 complex transcription unit of adenovirus encodes overlapping mRNAs (a to i) with different exon structures. The major mRNAs are a (approximately 40% of the total) and c (approximately 15%),...
Matsuo, T, Hashimoto, S, Wold, W S, Symington, J, Rankin, A, Green, M
The mRNA species encoded by early region 4 (E4) (map position [mp] 91.5 to 99.3) of adenovirus 2 were isolated from the polysomes of infected KB cells and were purified by hybridization to the cloned...
Role of early region 3 (E3) in pathogenesis of adenovirus disease.
Ginsberg, H S, Lundholm-Beauchamp, U, Horswood, R L, Pernis, B, Wold, W S, Chanock, R M, ...
The cotton rat Sigmodon hispidus has provided an animal model of adenovirus pneumonia that permits investigation of the viral gene products required to produce the disease and the molecular...
Region E3 encodes four major overlapping mRNAs with different splicing patterns. There are two poly(A) sites, an upstream site called E3A and a downstream site called E3B. We have analyzed virus...
Deutscher, S L, Bhat, B M, Pursley, M H, Cladaras, C, Wold, W S
Region E3 of adenovirus is a "complex" transcription unit: i.e. different mRNAs and proteins arise by differential RNA 3' end selection and differential splicing of the primary transcript. We are...
Lee, D C, Roeder, R G, Wold, W S
We have identified those sequences affecting the level of specific initiation of transcription in vitro from the EIII promoter of adenovirus 2. Mutants containing deletions in and around the...
We have identified adenovirus type 2 (Ad2)-induced early polypeptides (EPs) and have attempted to determine which EPs are coded by each of the four early gene blocks. [35S]methionine-labeled EPs were...
Green, M, Wold, W S, Brackmann, K H, Cartas, M A
A polypeptide of 55,000 daltons (55K) is linked, probably covalently, to the K' termini of adenovirus type 2 DNA. The 55K polypeptide is synthesized during early stages of infection (T. Yamashita, M....
Harter, M L, Shanmugam, G, Wold, W S, Green, M
(35S) methionine-labeled polypeptides synthesized by adenovirus type 2-infected cells have been analyzed by polyacrylamide gradient gel electrophoresis and autoradiography. Cycloheximide (CH) was...
Genome expression and mRNA maturation at late stages of productive adenovirus type 2 infection.
Wold, W S, Green, M, Brackmann, K H, Cartas, M A, Devine, C
RNA from adenovirus 2-infected KB cells was annealed in liquid with RNA in vast excess to viral heavy (l) and light (r) 32P-labeled DNA strands. Hybridization kinetics were analyzed by computer to...
Hoffman, B L, Ullrich, A, Wold, W S, Carlin, C R
We have used retrovirus-mediated gene transfer to introduce sequences encoding a 10,400-molecular-weight (10.4K) adenovirus protein previously shown to down regulate the receptor for epidermal growth...
Complex transcription units encode multiple mRNAs which arise by alternative processing of a common pre-mRNA precursor. It is not known how the pre-mRNA processing pathways are determined or...
White, E, Sabbatini, P, Debbas, M, Wold, W S, Kusher, D I, Gooding, L R
The adenovirus E1A and E1B proteins are required for transformation of primary rodent cells. When expressed in the absence of the 19,000-dalton (19K) E1B protein, however, the E1A proteins are...
Virus deletion mutants that affect a 3' splice site in the E3 transcription unit of adenovirus 2.
Bhat, B M, Brady, H A, Wold, W S
Five viable virus mutants were constructed with deletions near a 3' splice site located at nucleotide 2157 in the E3 transcription unit of adenovirus 2. The mutants were examined for splicing...
We mapped the location of the E3A RNA 3' end site in the E3 transcription unit of adenovirus 2. The procedure used was nuclease-gel analysis with 32P-labeled RNA probes. The poly(A) addition sites...
Jeng, Y H, Wold, W S, Sugawara, K, Gilead, Z, Green, M
The adenovirus type 2-coded single-stranded DNA binding protein (DBP) was shown to be a phosphoprotein and to exist in at least two forms that differ in mobility by sodium dodecyl...
Stewart, A R, Tollefson, A E, Krajcsi, P, Yei, S P, Wold, W S
The adenovirus type 2 and 5 E3 10,400- and 14,500-molecular-weight (10.4K and 14.5K) proteins are both required to protect some cell lines from lysis by tumor necrosis factor and to down-regulate the...
Hawkins, L K, Wilson-Rawls, J, Wold, W S
There is an open reading frame in the E3 transcription unit of adenovirus type 3 (Ad3) and Ad7 that could encode a protein of 16 kDa (16K protein). Ad3 and Ad7 are members of subgroup B of human...
Shisler, J, Duerksen-Hughes, P, Hermiston, T M, Wold, W S, Gooding, L R
The introduction of the adenovirus early region 1A (E1A) gene products into normal cells sensitizes these cells to the cytotoxic effects of tumor necrosis factor (TNF). Previous studies have shown...
Tollefson, A E, Scaria, A, Hermiston, T W, Ryerse, J S, Wold, L J, Wold, W S
Adenovirus (Ad) infection is concluded by assembly of virions in the cell nucleus followed by lysis of cells by an unknown mechanism. We have described an Ad nuclear membrane glycoprotein of 11,600...
Sparer, T E, Tripp, R A, Dillehay, D L, Hermiston, T W, Wold, W S, Gooding, L R
Products of human adenovirus (Ad) early region 3 (E3) inhibit both specific (cytotoxic T lymphocytes [CTLs]) and innate (tumor necrosis factor alpha [TNF-alpha]) immune responses in vitro. The E3...
Krajcsi, P, Dimitrov, T, Hermiston, T W, Tollefson, A E, Ranheim, T S, Vande Pol, S B, ...
Tumor necrosis factor (TNF) is an inflammatory cytokine that inhibits the replication of many viruses in cultured cells. We have reported that adenovirus (Ad) infection of TNF-resistant mouse cells...
Dimitrov, T, Krajcsi, P, Hermiston, T W, Tollefson, A E, Hannink, M, Wold, W S
We have reported that three adenovirus (Ad) proteins, named E3-10.4K/14.5K, E3-14.7K, and E1B-19K, independently inhibit tumor necrosis factor (TNF)-induced apoptosis in Ad-infected cells....
Hermiston, T W, Tripp, R A, Sparer, T, Gooding, L R, Wold, W S
Adenovirus E3-gp19K is a transmembrane glycoprotein, localized in the endoplasmic reticulum (ER), which forms a complex with major histocompatibility complex (MHC) class I antigens and retains them...
Hoffman, P, Rajakumar, P, Hoffman, B, Heuertz, R, Wold, W S, Carlin, C R
We have reported previously that human group C adenoviruses down-regulate the epidermal growth factor (EGF) receptor (EGF-R) (C. R. Carlin, A. E. Tollefson, H. A. Brady, B. L. Hoffman, and W. S. M....
Duerksen-Hughes, P J, Hermiston, T W, Wold, W S, Gooding, L R
Previous work by our laboratory and others has shown that mouse cells normally resistant to tumor necrosis factor can be made sensitive to the cytokine by the expression of adenovirus E1A. The E1A...
Ranheim, T S, Shisler, J, Horton, T M, Wold, L J, Gooding, L R, Wold, W S
The 14,700-Da protein (14.7K protein) encoded by the E3 region of adenovirus has previously been shown to protect mouse cells from cytolysis by tumor necrosis factor (TNF). Delineating the sequences...
Horton, T M, Ranheim, T S, Aquino, L, Kusher, D I, Saha, S K, Ware, C F, ...
A 14,700-kDa protein (14.7K) encoded by the E3 region of adenovirus has been shown to protect adenovirus-infected mouse C3HA cells from lysis by tumor necrosis factor (TNF) (L. R. Gooding, L. W....
Krajcsi, P, Tollefson, A E, Anderson, C W, Wold, W S
We previously reported that the adenovirus type 5 E3 14.5-kilodalton protein (14.5K) forms a complex with E3 10.4K and that both proteins are required to down-regulate the epidermal growth factor...
Gooding, L R, Aquino, L, Duerksen-Hughes, P J, Day, D, Horton, T M, Yei, S P, ...
Tumor necrosis factor (TNF) is a multifunctional immunoregulatory protein that is secreted by activated macrophages and is believed to have antiviral activities. We reported earlier that when mouse...
Tollefson, A E, Stewart, A R, Yei, S P, Saha, S K, Wold, W S
In adenovirus-infected cells, the epidermal growth factor receptor (EGF-R) is internalized from the cell surface via endosomes and is degraded, and the E3 10,400-dalton protein (10.4K protein) is...
Tollefson, A E, Scaria, A, Saha, S K, Wold, W S
We have reported that an 11,600-MW (11.6K) protein is coded by region E3 of adenovirus. We have now prepared two new antipeptide antisera that have allowed us to characterize this protein further....
Brady, H A, Scaria, A, Wold, W S
The E3 complex transcription unit of adenovirus encodes four major mRNAs (a, c, f, and h) and two minor (d and e) mRNAs with overlapping exons, alternative splice sites, and two polyadenylation...
Gooding, L R, Ranheim, T S, Tollefson, A E, Aquino, L, Duerksen-Hughes, P, Horton, T M, ...
We have reported that the E3 14,700-dalton protein (E3 14.7K protein) protects adenovirus-infected mouse C3HA fibroblasts against lysis by tumor necrosis factor (TNF) (L. R. Gooding, L. W. Elmore, A....
A 10,400-molecular-weight membrane protein is coded by region E3 of adenovirus.
Tollefson, A E, Krajcsi, P, Yei, S P, Carlin, C R, Wold, W S
Previous studies with adenovirus mutants have indicated that a 10,400-molecular-weight (10.4K) protein predicted to be coded by an open reading frame in region E3 of adenovirus functions to down...
Yei, S P, Chowdhury, S I, Bhat, B M, Conley, A J, Wold, W S, Batterson, W
We describe the characterization of the herpes simplex virus type 2 (HSV-2) gene encoding infected cell protein 32 (ICP32) and virion protein 19c (VP19c). We also demonstrate that the HSV-1...
Horton, T M, Tollefson, A E, Wold, W S, Gooding, L R
A 14.7-kilodalton protein (14.7K protein) encoded by the E3 region of group C adenoviruses has been shown to protect virus-infected fibroblasts from lysis by tumor necrosis factor (TNF) (L.R....
Early region E3 of adenovirus type 5 should encode at least nine proteins as judged by the DNA sequence and the spliced structures of the known mRNAs. Only two E3 proteins have been proved to exist,...
Region E3 of adenovirus encodes about 10 overlapping mRNAs with different spliced structures. The mRNAs are 5' coterminal and form two major 3'-coterminal families termed E3A and E3B. As a group, the...